2006 Annual Report 4d.Progress report. This report serves to document research conducted under a reimbursable agreement between ARS and Florida Department of Environmental Protection. Additional details of research can be found in the report in the parent project 6629-22000-009-00D “Development & evaluation of biological control agents for invasive species threatening the everglades & other natural and managed systems”. Invasive weeds have invaded the Everglades ecosystem decreasing the biodiversity of the natural areas, impacting agriculture and water management activities. Biological controls of these weeds will be accomplished by the introduction and spread of safe insects that reduce their vigor. This project sponsors release and redistribution of the Melaleuca quinquenervia biological control agents Oxyops vitiosa and Boreioglycaspis melaleucae. The mass production and redistribution of the two Melaleuca biological control agents totaled 208,485 agents to 104 sites. On Lygodium, 16,000 caterpillars of Austromusotima camptonozale were released on 12 lygodium-infested sites, including Everglades Tree Islands, Cypress Swamps and Pine Flatwoods, and additional Pine Flatwoods site was treated with 400 adult moths. Monitoring of Cypress and Pine Flatwoods sites found breeding populations at 4 sites indicating tentative establishment of this lygodium biological control agent. These biological controls will reduce the health and vigor of these invasive weeds and reduce their damage to natural areas and agriculture.