2006 Annual Report 4d.Progress report. This report serves to document research conducted under a reimbursable agreement between ARS and Dade County Department of Environmental and Resources Management. Additional details of research can be found in the report in the parent project 6629-22000-009-00D Development & evaluation of biological control agents for invasive species threatening the everglades & other natural and managed systems. A sap sucking insect called melaleuca psyllid was first released in south Florida in 2002 for biological control of melaleuca. These insects were mass reared in our screened facility for field releases throughout Dade County. For this project, we maintained several hundred potted-melaleuca plants in the field and screenhouse nurseries. Every month, we transferred 50-100 potted-plants to a second screenhouse and intermingled them with left-over plants from previous infestations which contained adult psyllids. After about 4-weeks, most melaleuca tips and old-leaves were covered with eggs, nymphs, and white flocculants. At this stage, we enclosed each potted plant in a fine screen to contain newly emerging adults. The total number of adults and ready-to-fly nymphs on plants inside individual screen were estimated, transported to the field, and released by removing screen. We left plants in the field by enclosing root-system in a plastic bag containing about 8-liters of water to keep the plant alive for a 2-week period to facilitate nymph development and adult transfer to the surrounding melaleuca trees. During 2005-2006, we released 261,456 ready-to-fly nymphs and adults of psyllids at 30 locations in Dade County. They have successfully established at all of these locations including in areas where they had suffered heavy loss during 2005 hurricane season. This melaleuca psyllid production, release, and monitoring activities will be continued for remainder of the project period to ensure insect distribution and establishment in all melaleuca-infested sites in Dade County.