2005 Annual Report 4d.Progress report. This report serves to document research conducted under a reimbursable agreement between ARS and Dade County Department of Environmental and Resources Management. Additional details of research can be found in the report for the parent project 6629-22000-008-00D Biological Control for Sustainable Management of Aquatic Weeds and Other Invasive Plants. A sap sucking insect called melaleuca psyllid was first released in south Florida in 2002 for biological control of melaleuca. In this project, we mass produced these insects in our Laboratory for field releases throughout Dade County. At any given time, we maintained several hundreds of melaleuca plants in a screenhouse for use in rearing psyllids. Every 2 months, we transferred 100-150 plants to a second screenhouse and inoculated them with adult psyllids. After few weeks, each new melaleuca tip and most leaves were covered with eggs, nymphs, and white flocculants. At this stage, we enclosed each potted plant in a fine screen to contain newly emerging adults. The total numbers of insects inside each screen were then estimated and plants were transported to the field where the insects were released by removing screen from the plants. During 2004-2005, we released 265,704 psyllid adults at 37 locations in Dade County and had 100% successful establishment at all of these locations. These psyllid production, release, and monitoring activities will be continued for remainder of the project period to ensure insect distribution and establishment in all melaleuca-infested sites in Dade County.