2004 Annual Report 4.What were the most significant accomplishments this past year? D. This report serves to document research conducted under a reimbursable agreement between ARS and Dade County Department of Environmental and Resources Management. Additional details of research can be found in the report for the parent project 6629-22000-008-00D Biological Control for Sustainable Management of Aquatic Weeds and Other Invasive Plants. A sap sucking insect called the melaleuca psyllid was first released in south Florida in 2002 for biological control of melaleuca. In this project, we mass produced insects in our Laboratory for field releases in Dade County. At any given time, we maintained several hundreds of melaleuca plants in a screenhouse for use in psyllid rearing. Every 2 months, we transferred 100-150 trees to a second screenhouse and inoculated them with adult psyllids. After few weeks, each new melaleuca tip and most leaves were covered with eggs, nymphs, and white flocculence. At this stage, individual plants were enclosed in a fine screen to contain newly emerging adults. The total numbers of insects inside each screen were estimated and plants were transported to the field where insects were released by removing the screen from the plants. During 2003-2004, we released 222,115 psyllid adults at 61 locations in Dade County of Florida. The psyllid production and release activities will be continued for remainder of the project period to ensure insect distribution to all infestation sites in Dade County.