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Research Project:
DEVELOPMENT OF COLD STORAGE TECHNOLOGY FOR MASS-REARED AND LABORATORY-COLONIZED INSECTS
Location: Insect Genetics and Biochemistry Research
Project Number: 5442-22000-040-00
Project Type:
Appropriated
Start Date: May 25, 2005
End Date: May 24, 2010
Objective:
Develop methods for short and long-term cold storage of beneficial and pest insects such as tephritid fruit flies, glassy-wing sharpshooter, Heliothine moths, and Mymarid wasps used in area wide sterile release and biocontrol programs; and for insects used in fundamental high impact research projects which generate or acquire numerous valuable genetic strains.
Approach:
Design short-term storage techniques by determining developmental and stage specific cold tolerance and susceptibility to manipulation of insect dormancy; Devise cryogenic procedures for long-term storage in liquid nitrogen by determining chilling tolerance, membrane permeability, cryoprotectant and post-storage recovery requirements.
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Last Modified: 11/07/2008
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