Project Number: 5358-21000-037-11
Project Type: Specific Cooperative Agreement

Start Date: Aug 30, 2005
End Date: Dec 31, 2008

Objective:
Relative cold tolerance of A. kolomikta accessions will be evaluated by testing dormant scionwood with two runs over two winter seasons primarily using material from Minnesota and supplemented by material obtained from the NCGR in December or January and acclimated in Minnesota prior to the freezing tests. Fruit and plants of the accessions will be evaluated for the following traits in orchards at Victoria or Roseville, Minnesota as fruit are available over the evaluation period.

Approach:
Scionwood Collection and Storage; Actinidia kolomikta scionwood will be collected from most accessions in early December from the University of Minnesota Horticultural Research Center (HRC) in Victoria, Minnesota and supplemented as needed from an auxiliary collection in Roseville, Minnesota. Additional material will be obtained from the USDA- NCGR in Corvallis, Oregon. The scionwood will then be stored outdoors in a plastic container in a shaded location to emulate natural temperature conditions. Scionwood Preparation; In preparation for the respective freeze tests, one bundle of the A. kolomikta scionwood will be unpacked and the sticks cut into 5 cm segments of approximately the same diameter. A total of five replicate pieces will be cut for each temperature increment. For the freeze tests, one scionwood segment will be randomly selected from each sealable bag and a thermocouple inserted into the pith. The bag will then be resealed and the remaining scionwood pieces will be distributed horizontally in a plane within the bag. The bags will be ¿sandwiched¿ between the freezer bottom and an overlying metal plate for increased thermal heat transfer. Documents SCA with U. of Minnesota. Formerly 5358-21000-036-17S (7/08).