2007 Annual Report 1a.Objectives (from AD-416) Breeding soybean for higher yield and nutrition. 1b.Approach (from AD-416) Soybean accessions will be planted during the winter months in Isabela, Puerto Rico for seed increase. 3.Progress Report This report documents research conducted under a reimbursable agreement between ARS and North Carolina State University. Additional details of research can be found in the report for the in-house associated project 6635-21000-044-00D, Genetic Resource Management of Tropical/Subtropical Genetic Resources and Associated Information. This project relates to objective 1 of the in-house associated project. First generation (F1) soybean seed from cross pollinations were grown for seed increase. Cross pollinations were made with the following breeding objectives: yield increase, increase protein and oil in seeds, improve fatty acid composition of seed oil, increase drought tolerance, increase genetic diversity, and decrease the phytic acid content of soybeans. In addition to these materials, breeding populations for the aforementioned traits were advanced an additional generation. Materials with cultivar potential, were grown to increase seed so that they could be yield tested in multiple environments during the summer season. For the oil quality research, single soybean plants were harvested and screened for increased oleic acid content. Activities in this project are monitored through frequent visits to experimental plots, taking digital images of experimental plots and providing those to collaborator, and frequent communications with collaborator by phone and email.