2006 Annual Report 4d.Progress report. This report serves to document research conducted under a specific cooperative agreement between ARS and University of Wisconsin - Madison. Additional details of research can be found in the report for the parent CRIS 3655-21000-039-00D, Conservation and Utilization of Potato Genetic Resources. Hybrids were made and multiplied for incorporating high tuber calcium trait from wild species into cultivated background. An advanced generation combining very high yield and frost resistance was selected, multiplied and is being tested in several US states and in Peru. We conducted more detailed characterization of the anti-cancer proteins Potato Carboxypeptidase Inhibitor (PCI) in tuber flesh, and generated a wide array of materials for future testing. These investigations join expertise in physiology and genetics to address three critical problems of the potato industry:. 1)improved calcium for better tuber quality,. 2)cold tolerance for maintaining productivity in the face of environmental stress, and. 3)increasing the nutritional value of potato to increase appeal to consumers.