1a.Objectives (from AD-416)
The objective of this research is to set up a Pilot Program consisting of two dairy herds to focus on the protocol development, laboratory set-up, program logistics, and database and bio-bank development needed to evaluate the impact of intervention strategies on Johne's disease dynamics, milk and beef quality (particularly with respect to zoonotic bacterial pathogens), economics and sustainability through intensive longitudinal follow up of selected research/demonstration dairy farms. Long term goals are to validate intervention strategies to support BMP's and to optimize intervention and monitoring strategies given the constraints on time, labor and financial resources in modern dairy herds. In addition, a national resource bank (data and biological specimens on well-characterized animals) will be created for current and future monitoring and research on dairy cattle diseases. Emphasis will be on longitudinal data collection on endemic infectious diseases of public health and animal health concern in dairy herds.

1b.Approach (from AD-416)
Pathogens are of increasing concern on dairy farms and in dairy products. The production of safe and wholesome food from US farms requires control of the production process on the farm. Specific focus areas in this process are biosecurity, food safety and animal health. To be able to scientifically support regional process control programs there is a need for longitudinal research on commercial dairy farms throughout the United States. In this collaboration, Cornell University, The Pennsylvania State University, University of Vermont, and the University of Pennsylvania, all participants in the Regional Dairy Quality Management Alliance (RDQMA), and the USDA's Environmental Microbial Safety Laboratory (EMSL) will investigate the sites on operating dairy farms that act as reservoirs for pathogenic microorganisms that affect animal health and that decrease product quality because of their zoonotic nature. Several farms will be identified as candidates for this investigation and 3 will be chosen for extensive sampling based on the previous occurrence of Johne's disease and/or Salmonella infection. Serum, feces, bulk tank milk, and environmental samples (water, bird and rodent feces, feed, etc.) will be taken on each farm. In addition, tissue samples will be obtained from carcasses of culled animals. Samples will be distributed among the university and ARS researchers for analysis to determine the presence of Mycobacterium avium paratuberculosis (the causative agent of Johne's disease in cattle) and for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes (human food-borne pathogens of concern in dairy products). This research will be the first to attempt a comprehensive analysis of both Johne's disease and food-borne pathogens on working dairy farms. It will allow us to determine a baseline for these organisms on two farms and set the stage for investigation of the effect of interventions, in the form of best management practices (BMPs), on animal health and product quality.

3.Progress Report
This report documents research conducted under a Specific Cooperative Agreement between ARS and the University of Pennsylvania. Additional details of the research can be found in the report for the parent project 1265-32000-078-00D "Dairy Management Practices and the Transmission of Zoonotic Pathogens in Milk." Analysis of samples from participant farms for the presence of Mycobacterium avium paratuberculosis proceeded under a new schedule required by additional testing needed to define the supershedder phenomenon detected. The sampling rate on the participant farm with low or no Johne’s disease incidence was halved in order to accomadate increased sampling on other farms. Data are reported to the project coordinator as required.

The ADODR has monitored activities associated with this project through infrequent phone calls and emails. An annual meeting of all major participants in this project was held once this period. ADODR reviews SF 269A forms.