The goal of my research is to identify weak points in the nematode life cycle that may be exploited in the design of environmentally safe, highly specific control strategies with emphasis on nematode pathogens of alfalfa and soybean, and their rotation crops.
Objectives include:
Development of novel methodologies for identifying and classifying plant-parasitic nematodes, including PCR, diagnostic assays and phylogenies based upon ribosomal, mitochondrial, or new protein coding genes (such as Hsp90).
Discovery and characterization of novel molecular targets from nematodes or host plants that may be manipulated by transgenic methods for the purpose of bioengineering nematode-resistant cultivars.
Expected outcomes:
Novel molecular markers and assays that provide rapid, accurate, and reliable identifications of nematode species and pathotypes will aid regulatory agencies and other nematologists in making scientifically-based management and quarantine decisions. These identification tools will facilitate the continued export of billions of dollars of agricultural products while preventing the introduction or spread of dangerous pathogens.
Identification and characterization of genes involved in essential nematode life processes will uncover novel targets for nematode control. Likewise, the identification of host plant genes expressed in response to nematode infection will reveal additional targets or markers that may be used to increase host resistance to nematodes.
Second-stage juvenile of the Northern Root-Knot Nematode, a pest of alfalfa