Jo Ann Wise
MCB Division of Molecular and Cellular Biosciences
BIO Directorate for Biological Sciences
Start Date:
September 1, 2003
Expires:
August 31, 2008 (Estimated)
Awarded Amount to Date:
$1720000
Investigator(s):
Arthur Hunt aghunt00@uky.edu (Principal Investigator)
Qingshun Li (Co-Principal Investigator)
Sponsor:
University of Kentucky Research Foundation
201 Kinkead Hall
Lexington, KY 40506 859/257-9420
NSF Program(s):
GENES AND GENOME SYSTEMS
Field Application(s):
Program Reference Code(s):
BIOT, 9150, 9109, 1684, 1154
Program Element Code(s):
1112
ABSTRACT
The polyadenylation of messenger RNAs (mRNAs) is an important step in the process of gene expression in plants. Polyadenylation adds an essential element to the mRNA and helps to coordinate the synthesis of a messenger RNA with its ultimate functioning in protein synthesis. Consequently, the protein subunits that make up the polyadenylation machinery are expected to play important roles in plant growth. The goals of this project are to determine the collective function of the set of Arabidopsis genes that encode subunits of the polyadenylation machinery (this set, including Arabidopsis genome identifiers, can be found at http://www.uky.edu/~aghunt00/polyA2010.html and http://www.users.muohio.edu/liq/arabidopsis2010.html). This will include a documentation of the phenotypes of plants that have had one or more subunit removed (by mutation or gene silencing) and a determination of the complete set of protein-protein interactions involving the various subunits. The results will be disseminated via peer-reviewed publication as well as through web pages managed by the principal investigators (http://www.uky.edu/~aghunt00/polyA2010.html and http://www.users.muohio.edu/liq/arabidopsis2010.html). In addition, mutant Arabidopsis stocks will be deposited with the appropriate stock center(s). The research will contribute to a goal of the 2010 Project ("to determine the function of all genes of a reference species within their cellular, organismal and evolutionary contexts by the year 2010") by cataloguing mutant phenotypes, by defining the network of interactions amongst Arabidopsis polyadenylation factor subunits, and by identifying proteins of unknown function that interact with the polyadenylation machinery.
The research will greatly increase the current understanding of mRNA 3' end formation in higher plants. As such, it will enhance the ability to tailor foreign genes for expression in transgenic plants, and thereby develop improved crops (as well as novel alternative economic opportunities) for farmers. It will also lend significant insight into the evolution of mRNA polyadenylation. The research products (cloned cDNA, antibodies, plant lines etc.) will be made available to the research community at large. The research will contribute to the training of two postdoctoral scholars, four graduate students, and as many as twelve undergraduates. The latter will be drawn from highly-regarded and diverse programs at the University of Kentucky and Miami University.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
(Showing: 1 - 19 of 19).
Addepalli B, Hunt AG.
"A novel endonuclease activity associated with the Arabidopsis ortholog of the 30-kDa subunit of cleavage and polyadenylation specificity factor.,"
Nucleic Acids Research,
v.35,
2007,
p. 4453.
Addepalli, B.;Hunt, A. G.;.
"Ribonuclease activity is a common property of Arabidopsis CCCH-containing zinc-finger proteins,"
FEBS Lett,
v.582,
2008,
p. 2577-82.
Addepalli, B.;Hunt, A. G.;.
"Redox and heavy metal effects on the biochemical activities of an Arabidopsis polyadenylation factor subunit,"
Arch Biochem Biophys,
v.473,
2008,
p. 88-95.
Addepalli, B; Meeks, LR; Forbes, KP; Hunt, AG.
"Novel alternative splicing of mRNAs encoding poly(A) polymerases in Arabidopsis,"
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION,
v.1679,
2004,
p. 117
- 128.
Balasubrahmanyam Addepalli and Arthur G. Hunt.
"The interaction between two Arabidopsis polyadenylation factor subunits involves an evolutionarily-conserved motif and has implications for the assembly and function of the polyadenylation complex.,"
Protein and Peptide Letters,
v.15,
2008,
p. 76.
Chun Liang, Yuansheng Liu, Lin Liu, Adam C. Davis, Yingjia Shen and Qingshun Quinn Li.
"Expressed Sequence Tags With cDNA Termini: Previously Overlooked Resources for Gene Annotation and Transcriptome Exploration in Chlamydomonas reinhardtii,"
Genetics,
v.179,
2008,
p. 83.
Delaney, KJ; Xu, RQ; Zhang, JX; Li, QQ; Yun, KY; Falcone, DL; Hunt, AG.
"Calmodulin interacts with and regulates the RNA-binding activity of an Arabidopsis polyadenylation factor subunit,"
PLANT PHYSIOLOGY,
v.140,
2006,
p. 1507
- 1521.
Forbes, KP; Addepalli, B; Hunt, AG.
"An Arabidopsis Fip1 homolog interacts with RNA and provides conceptual links with a number of other polyadenylation factor subunits,"
JOURNAL OF BIOLOGICAL CHEMISTRY,
v.281,
2006,
p. 176
- 186.
Guoli Ji, Xiaohui Wu, Jianti Zheng, Yingjia Shen and Q. Quinn Li..
"Modeling Plant mRNA Poly(A) sites: Software Design and Implementation.,"
Journal of Computational and Theoretical Nanoscience, 4:1365-1368.,
v.4,
2007,
p. 1365.
Hunt, A. G.;Xu, R.;Addepalli, B.;Rao, S.;Forbes, K. P.;Meeks, L. R.;Xing, D.;Mo, M.;Zhao, H.;Bandyopadhyay, A.;Dampanaboina, L.;Marion, A.;Von Lanken, C.;Li, Q. Q.;.
"Arabidopsis mRNA polyadenylation machinery: comprehensive analysis of protein-protein interactions and gene expression profiling,"
BMC Genomics,
v.9,
2008,
p. 220.
Jingxian Zhang, Balasubramanyam Addepalli, Kil-Young Yun1, Arthur G. Hunt, Ruqiang Xu, Suryadevara Rao, Qingshun Q. Li, Deane L. Falcone.
"A Polyadenylation Factor Subunit Implicated in Regulating Oxidative Signaling in Arabidopsis thaliana.,"
PLoS ONE,
v.3,
2008,
p. e2410.
Loke, JC; Stahlberg, EA; Strenski, DG; Haas, BJ; Wood, PC; Li, QQ.
"Compilation of mRNA polyadenylation signals in Arabidopsis revealed a new signal element and potential secondary structures,"
PLANT PHYSIOLOGY,
v.138,
2005,
p. 1457
- 1468.
Ruqiang Xu and Q. Quinn Li.
"Streamline cloning of genes into binary vectors in Agrobacterium via the Gateway® TOPO vector system.,"
Plant Methods,
v.4,
2008,
p. 4.
Shen, Y.;Ji, G.;Haas, B. J.;Wu, X.;Zheng, J.;Reese, G. J.;Li, Q. Q.;.
"Genome level analysis of rice mRNA 3'-end processing signals and alternative polyadenylation.,"
Nucleic Acids Res,
v.36,
2008,
p. 3150-61.
Shen, Y.;Liu, Y.;Liu, L.;Liang, C.;Li, Q. Q.;.
"Unique features of nuclear mRNA poly(A) signals and alternative polyadenylation in Chlamydomonas reinhardtii,"
Genetics,
v.179,
2008,
p. 167-76.
Xing, D.;Zhao, H.;Xu, R.;Li, Q. Q.;.
"Arabidopsis PCFS4, a homologue of yeast polyadenylation factor Pcf11p, regulates FCA alternative processing and promotes flowering time,"
Plant J,
v.54,
2008,
p. 899-910.
Xu, RQ; Ye, XF; Li, QSQ.
"AtCPSF73-II gene encoding an Arabidopsis homolog of CPSF 73 kDa subunit is critical for early embryo development,"
GENE,
v.324,
2004,
p. 35
- 45.
Xu, RQ; Zhao, HW; Dinkins, RD; Cheng, XW; Carberry, G; Li, QQ.
"The 73 kD Subunit of the cleavage and polyadenylation specificity factor (CPSF) complex affects reproductive development in Arabidopsis,"
PLANT MOLECULAR BIOLOGY,
v.61,
2006,
p. 799
- 815.
(Showing: 1 - 19 of 19).
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