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Volume 12, Number 7, July 2006 Triple Reassortant H3N2 Influenza A Viruses, Canada, 2005Christopher W. Olsen,* Alexander I. Karasin,* Suzanne
Carman,† Yan Li,‡ Nathalie Bastien,‡ Davor Ojkic,† David Alves,§ George
Charbonneau,¶ Beth M. Henning,# Donald E. Low,** Laura Burton,** and George
Broukhanski** |
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Figure 1. Nucleotide phylogram for the hemagglutinin (HA) genes of A/Swine/Manitoba/12707/05, A/Swine/Alberta/14722/05, A/Swine/British Columbia/28103/05, A/Swine/Ontario/33853/05, A/Turkey/Ontario/31232/05, A/Ontario/RV1273/05, and related reference viruses. The evolutionary relationships among these viruses were estimated by the method of maximum parsimony (PAUP software, version 4.0b10) with a bootstrap resampling method (200 replications) and a fast heuristic search algorithm. Numbers at the nodes of the phylograms indicate the bootstrap confidence levels. Horizontal-line distances are proportional to the minimum numbers of nucleotide changes needed to join nodes and gene sequences. Vertical lines are present to space the branches and labels. The designations I, II, and III identify clusters of viruses previously defined among triple reassortant swine viruses in the United States (4). The viruses described in this report and related viruses are proposed to represent a new cluster IV group of viruses. GenBank accession numbers for the sequences of all reference viruses are provided in parentheses after the virus names. |
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