Projects

RESEARCH TO DEVELOP STRATEGIES AND TECHNOLOGIES FOR PRESERVING PLANT GENETIC DIVERSITY IN EX SITU GENEBANKS Appropriated (D)   Accession Number: 414258

NONINVASIVE METHODS TO PREDICT SEED STORAGE BEHAVIOR AND MONITOR VIABILITY OF SPECIES COLLECTED ON NPS LANDS Reimbursable (R)   Accession Number: 412751

KINETICS OF SEED DETERIORATION IN DIVERSE LINES OF RYE, WHEAT AND TRITICALE Specific Cooperative Agreement (S)   Accession Number: 414235

MECHANISMS OF GENETIC CHANGE DURING EX SITU CONSERVATION OF RYE GERMPLASM Specific Cooperative Agreement (S)   Accession Number: 408381

Publications

Biophysical Approaches to Measure and Predict Seed Longevity - (Abstract)

Walters, C.T. 2008. Biophysical approaches to measure and predict seed longevity. 9th International Society for Seed Science Conference on Seed Biology. July 6-11, 2008. Olsztyn, Poland. pp. 88. Meeting Abstract.

TEMPERATURE AND MOISTURE CONTROL OF SEED AGING IN RYE - (Abstract)

Walters, C.T., Niedziekski, M., Hill, L.M., Wheeler, L.J., Puchalski, J. 2008. Temperature and moisture control of seed aging in rye. 9th International Society for Seed Science Conference on Seed Biology. July 6-11, 2008. Olsztyn, Poland. pp. 281. Meeting Abstract.

WATER CONTENT-TEMPERATURE INTERACTIONS REGULATE SEED AGING - (Abstract)

Walters, C.T., Hill, L.M., Wheeler, L.J., Rao, N.K., Hu, X., Dulloo, M.E., Engels, J.M.M. 2008. Water content-temperature interactions regulate seed aging. 9th International Conference on Seed Biology. July 6-11, 2008. Olsztyn, Poland. pp. 280. Meeting Abstract

VARIATION OF SEED AGING RATES AMONG LINES OF WHEAT, RYE AND TRITICALE IN RESPONSE TO STORAGE HUMIDITY - (Abstract)

Niedziekski, M.,Waters, C.T., Luczak, C.,L.M. Hill, Puchalski, J. 2008. Variation of seed aging rates among lines of wheat, rye and triticale in response to storage humidity. 9th International Society for Seed Science Conference on Seed Biology. July 6-11, 2008. Olsztyn, Poland. pp. 94-95. Meeting Abstract.

SEED DETERIORATION INCREASES IN THE PRESENCE OF VOLATILES - (Abstract)

Mira, S., Walters, C.T., Hill, L.M., Estrelles, E., Gonzalez-Benito, M.E. 2008. Seed deterioration increases in the presence of volatiles. 9th International Society for Seed Science Conference on Seed Biology. July 6-11, 2008. Olsztyn, Poland. pp. 89. Meeting Abstract.

STORAGE BEHAVIOR OF SEEDS FROM NATIVE HAWAIIAN TAXA - (Abstract)

Weisenberger, L. Hill, L.M., Yoshinaga, A., Wood, K., Walters, C.T. 2008. Storage behavior of seeds from native hawaiian taxa. 9th International Conference on Seed Biology. July 6-11, 2008. Olsztyn, Poland. pp. 281-282. Meeting Abstract.

PHYSIOLOGICAL ASPECTS OF SEED DEVELOPMENT IN A HAWAIIAN PALM - (Abstract)

Perez, H., Hill, L.M., Criley, R., Baskin, C., Drake, D., Kobayashi, K., Maunder, M., Walters, C.T. 2008. Physiological aspects of seed development in a hawaiian palm. 9th International Conference on Seed Biology. July 6-11, 2008. Olsztyn, Poland. pp. 135. Meeting Abstract.

Genebanks in the post-genomic age: emerging roles and anticipated uses - (Peer Reviewed Journal)

Walters, C.T., Volk, G.M., Richards, C.M. 2008. Genebanks in the post-genomic age: emerging roles and anticipated uses. Biodiversity Issues 9:68-71.

Materials used for Seed Storage Containers: Response - (Peer Reviewed Journal)

Walters, C. 2007. Materials used for seed storage containers: Response. Seed Science Research 17:233-242.

Biophysical Approaches to Measure and Predict Seed Longevity - (Abstract)

Walters, C. 2007. Biophysical Approaches to Measure and Predict Seed Longevity. Meeting Abstract for the First symposium of Translational Biology in Seeds. September 17-20, 2007, Davis, California. pp. 12.

Longevity of Native Wildflower Seeds - (Abstract)

Walters, C.T. 2007. Longevity of Native Wildflower Seeds. Meeting Abstract for the Wildflower Symposium. July 19-20, 2007, Orlando, Flordia. pp. 16.

Longevity of Native Wildflower Seeds - (Proceedings/Symposium)

Walters, C.T. 2007. Longevity of Native Wildflower Seeds. Symposium Proceedings and oral presentation for the Wildflower Symposium. July 19-20, 2007, Orlando, Flordia.

Structural Dynamic and Desiccation Damage in Plant reproductive Organs - (Book/Chapter)

Walters, C. and K.L. Koster. 2007. Structural Dynamic and Desiccation Damage in Plant reproductive Organs. p. 251-280. In Jenks, M.A. and Wood A. (eds)Plant Desiccation Tolerance. Blackwell publishing, Oxford, U.K.

Glass formation, glass fragility, molecular mobility and longevity of germplasm stored at cryogenic temperatures. - (Abstract)

Walters, C.T. 2007. Glass formation, glass fragility, molecular mobility and longevity of germplasm stored at cryogenic temperatures. Meeting abstract for the 44th Annual Meeting of the Society for Cryobiology. July 28 - August 1, 2007, Lake Louise, Canada. pp. 44.

Shoot tip structural and biophysical responses to plant vitrification solution 2 (PVS2) exposure - (Abstract)

Volk, G.M., Caspersen, A.M., Walters, C.T. 2007. Shoot tip structural and biophysical responses to plant vitrification solution 2 (PVS2) exposure. Meeting abstract for the 44th Annual Meeting of the Society for Cryobiology. July 28 - August 01, 2007, Lake Louise, Canada. pp. 45.

CALORIMETRIC PROPERTIES OF WATER AND TRIACYLGLYCEROLS IN FERN SPORES RELATING TO STORAGE AT CRYOGENIC TEMPERATURES - (Peer Reviewed Journal)

Ballesteros, D., Walters, C.T. 2007. Calorimetric properties of water and triacylglycerols in fern spores relating to storage at cryogenic temperatures. Cryobiology 55:1-9.

Hydration of Cuphea seeds containing crystallized triacylglycerols - (Peer Reviewed Journal)

Volk, G.M., Crane, J., Caspersen, A.M., Kovach, D.A., Gardner, C.A., Walters, C.T. 2007. Hydration of Cuphea seeds containing crystallized triacylglycerols. Functional Plant Biology 34:360-367.

About the Limited Benefit of Water Content and Temperature on Orthodox Seed Longevity - (Proceedings/Symposium)

Walters, C. 2007. About the Limited Benefit of Water Content and Temperature on Orthodox Seed Longevity. Symposium Proceedings for the 5th Workshop on Desiccation of Plants. January 14-24, 2007, Durban, South Africa. pp. 34.

Desiccation sensitivity of white and black (red) oak embryonic axes - (Proceedings/Symposium)

Chmielarz, P., C. Walters. 2007. Desiccation sensitivity of white and black (red) oak embryonic axes. Symposium Proceedings for the 5th Workshop on Desiccation of Plants. January 14-24, 2007, Durban, South Africa. pp. 38.

The Encyclopedia of Seeds: Science Technology and Uses - (Other)

Walters, C.T. 2006. Accelerated Aging. 5. Arrhenius Plot. 23. Antioxidants.17. Deterioration and Longevity. 137-143. Glass. 295-296. Harrington¿s Thumb Rules. 322. Hydration Force Explanation. 341. Hysteresis. 343. Maillard Reaction. 389-390. Phase Transition. 498-499. Reactive Oxygen Species. 570. Superoxide Dismutase. 695. Water Binding. 749. Water Replacement Hypothesis. 751. In M. Black, J.D. Bewley and P, Halmer (eds.) Encyclopedia of Seeds: Science, Technology and Uses. CABI, Wallingford, Oxfordshire, UK.

WATER PROPERTIES IN FERN SPORES: SORPTION CHARACTERISTICS RELATING TO WATER AFFINITY, GLASSY STATES AND STORAGE STABILITY - (Peer Reviewed Journal)

Ballesteros, D., Walters, C.T. 2007. Water properties in fern spores: sorption characteristics relating to water affinity, glassy states and storage stability. Journal of Experimental Botany 58:1185-1196.

Cryopreservation of Recalcitrant (i.e. Desiccation-Sensitive) Seeds - (Book/Chapter)

Walters C, J. Wesley-Smith, J. Crane, L.M. Hill, P. Chmielarz, N.W. Pammenter and P. Berjak. 2008. Cryopreservation of recalcitrant (i.e. desiccation-sensitive) seeds. pp. 465-484. In B. Reed (ed.) Plant Cryopreservation: A practical guide. Springer Publishing, New York, NY.

TRIACYLGLYCEROL PHASE AND SEED STORAGE BEHAVIOR - (Abstract)

Walters, C.T., Crane, J., Volk, G.M. 2006. Triacylglycerol phase and seed storage behavior. 17th International Symposium on Plant Lipids. July 16-21, 2006, East Lansing, Michigan. p. 67. Meeting Abstract.

MASSIVE CELLULAR DISRUPTION OCCURS DURING EARLY IMBIBITION OF CUPHEA SEEDS CONTAINING CRYSTALLIZED TRIACYLGLYCEROLS - (Peer Reviewed Journal)

Volk, G.M., J. Crane, A.M. Caspersen, L.M. Hill, C.A. Gardner and C. Walters. 2006. Massive cellular disruption occurs during early imbibition of Cuphea seeds containing crystallized triacylglycerols. Planta 224:1415-1426.

COFFEE SEED PHYSIOLOGY - (Peer Reviewed Journal)

Eira, M.T.S., E.A. da Silva, R.D. de Castro, S. Dussert, C. Walters, J.D. Bewley and H.W.M. Hilhorst. 2006. Coffee seed physiology. Brazilian Journal of Plant Physiology 18:149-163.

Capturing genetic diversity of wild populations for ex situ conservation: Texas wild rice (Zizania texana) as a model - (Peer Reviewed Journal)

Richards, C.M., Antolin, M.F., Reilley, A., Poole, J., Walters, C.T. Capturing genetic diversity of wild populations for ex situ conservation: Texas wild rice (Zizania texana) as a model. Genetic Resources and Crop Evolution 54:837-848.

THE UTILITY OF AGED SEEDS IN DNA BANKS - (Peer Reviewed Journal)

Walters, C., A.A. Reilley, P.A. Reeves, J. Baszczak, and C.M. Richards. 2006. The utility of aged seeds in DNA banks. Seed Science Research 16:169-178.

PLANT VITRIFICATION SOLUTION 2 (PVS2) LOWERS WATER CONTENT AND ALTERS FREEZING BEHAVIOR IN SHOOT TIPS DURING CRYOPROTECTION - (Peer Reviewed Journal)

Volk, G.M. and C. Walters. 2006. Plant vitrification solution 2 lowers water content and alters freezing behavior in shoot tips during cryoprotection. Cryobiology 52:48-61.

PLATFORMS FOR DNA BANKING - (Review Article)

Walters, C., and Hanner, R. 2006. Platforms for DNA Banking. p. 25-35. In M. Carmen de Vicente and Meike S. Andersson (eds.) Issues in Genetic Resources DNA Banks - Providing Novel Options for Genebanks? International Plant Genetic Resources Institute, Rome, Italy.

TRIACYLGLYCEROL PHASE AND 'INTERMEDIATE' SEED STORAGE PHYSIOLOGY: A STUDY OF CUPHEA CARTHAGENENSIS - (Peer Reviewed Journal)

Crane, J., Kovach, D.A., Gardner, C.A., Walters, C.T. 2005. Triacylglycerol phase and 'intermediate' seed storage physiology: a study of cuphea carthagenensis. Planta 223:1081-1089.

DYING WHILE DRY: KINETICS AND MECHANISMS OF DETERIORATION IN DESICCATED ORGANISMS - (Peer Reviewed Journal)

Walters, C., L.M. Hill and L.J. Wheeler. 2005. Dying while dry: kinetics and mechanisms of deterioration in desiccated organisms. Integrative and Comparative Biology 45(5):751-758.

SURVIVAL OF FOUR ACCESSIONS OF ANIGOZANTHOS MANGLESII (HAEMODORACEAE) SEEDS FOLLOWING EXPOSURE TO LIQUID NITROGEN - (Peer Reviewed Journal)

Merritt, D.J., Touchell, D.H., Senaratna, T., Dixon, K.W., Walters, C. 2005. Survival of four accessions of Anigozanthos manglesii (Haemodoraceae) seeds following exposure to liquid nitrogen. CryoLetters 26:121-130.

ORGANIZATION OF LIPID RESERVES IN COTYLEDONS OF PRIMED AND AGED SUNFLOWER SEEDS - (Peer Reviewed Journal)

Walters, C., P. Landre, L.M. Hill, F. Corbineau and C. Bailly. 2005. Organization of lipid reserves in cotyledons of primed and aged sunflower seeds. Planta 222:397-407.

WATER PROPERTIES AND CELL LONGEVITY - (Book/Chapter)

Walters, C. 2005. Water properties and cell longevity. In P. Buera, J. Welti-Chanes, P. Lillford and H. Corti (eds). Water Properties of Food, Pharmaceutical and Biological Materials. CRC Press, Boca Raton, FL. p. 191-204.

LONGEVITY OF SEEDS STORED IN A GENEBANK: SPECIES CHARACTERISTICS - (Peer Reviewed Journal)

Walters, C.T., Wheeler, L.J., Grotenhuis, J.A., 2005. Longevity of seeds stored in a genebank: Species characteristics. Seed Science Research 15:1-20.

DYING WHILE DRY: KINETICS AND MECHANISMS OF DETERIORATION IN DESICCATED ORGANISMS - (Abstract)

Walters, C. 2005. Dying while dry: kenetics and mechanisms of deterioration in desiccated organisms. Annual Meetings of the Society of Integrative and Comparative Biology. January 4-8, 2004, San Diego, California. pp. 246.

LONGEVITY OF SEEDS STORED IN A GENEBANK: SPECIES CHARACTERISTICS - (Abstract)

Walters, C.T., Wheeler, L.J., Grotenhuis, J.A. 2004. Longevity of seeds stored in a genebank: species characteristics. Proceedings of CSSA Annual Meetings, Seattle, Washington October 31 - September 11, 2004.

CRYOPRESERVATION OF MALUS GERMPLASM USING A WINTER VEGETATIVE BUD METHOD: RESULTS FROM 1915 ACCESSIONS - (Peer Reviewed Journal)

Towill L.E., P.L. Forsline, C. Walters, J.W. Waddell and J. Laufmann. 2004. Cryopreservation of Malus germplasm using a winter vegetative bud method: results from 1915 accessions. CryoLetters 25:323-334.

MICROSATELLITE PRIMERS FOR TEXAS WILD RICE AND A PRELIMINARY TEST OF THE IMPACT OF CRYOGENIC STORAGE ON ALLELE FREQUENCY AT THESE LOCI. - (Peer Reviewed Journal)

Richards C.M., A. Reilley, D.H. Touchell, M.F. Antolin and C. Walters. Microsatellite primers for Texas wild rice (Zizania texana), and a preliminary test of the impact of cryogenic storage on allele frequency at these loci. Conservation Genetics 5:853-859.

THE STATUS OF WATER IN PRESERVED CELLS AND WHAT IT MEANS FOR LONG-TERM VIABILITY - (Abstract)

Walters, C. 2004. The status of water in preserved cells and what it means for long-term viability. 9th International Symposium on the Properties of Water, September 25-30, 2004, Mar del Plata, Argentina. pp. 121.

GUIDELINES FOR SEED STORAGE - (Book/Chapter)

Walters, C. 2004. Guidelines for Seed Storage. pp.442-453. In E. Guerrant, K. Havens and M. Maunder (eds) Ex Situ Plant Conservation: Supporting Species Survival in the Wild. Island Press, Covela, CA.

PRINCIPLES FOR PRESERVING GERMPLASM IN GENE BANKS - (Book/Chapter)

Walters, C. 2004. Principles for Preserving Germplasm in Genebanks. pp.113-138. In E. Guerrant, K. Havens and M. Maunder (eds) Ex Situ Plant Conservation: Supporting Species Survival in the Wild. Island Press, Covela, CA.

LONGEVITY OF CRYOGENICALLY-STORED SEEDS - (Peer Reviewed Journal)

Walters C., L.J. Wheeler and P.C. Stanwood. 2004. Longevity of cryogenically-stored seeds. Cryobiology 48:229-244.

TEMPERATURE-DEPENDENCY OF MOLECULAR MOBILITY IN PRESERVED SEEDS - (Peer Reviewed Journal)

Walters, C. 2004. Temperature-dependency of molecular mobility in preserved seeds. Biophysical Journal 86:1253-1258.

THE INFLUENCE OF WATER CONTENT, COOLING AND WARMING RATE UPON SURVIVAL OF EMBRYONIC AXES OF PONCIRUS TRIFOLATA (L.) - (Peer Reviewed Journal)

Wesley-Smith, J., C. Walters, P. Berjak and N.W. Pammenter. 2004. The influence of water content, cooling and warming rate upon survival of embryonic axes of Poncirus trifolata (L.). CryoLetters 25:129-138.

NON-EQUILIBRIUM COOLING OF PONCIRUS TRIFOLIATA EMBRYONIC AXES AT VARIOUS WATER CONTENTS - (Peer Reviewed Journal)

Wesley-Smith, J., C. Walters, P. Berjak and N.W. Pammenter. 2004. Non-equilibrium cooling of Poncirus trifoliate (L.) embryonic axes at various water contents. CryoLetters 25:121-128.

LONGEVITY AND GENETIC STABILITY OF CRYOPRESERVED MATERIALS - (Abstract)

Walters, C. 2003. Longevity and Genetic stability of cryopreserved materials. International Society for Biological and Environmental Repositories Newsletter, May 4-7, 2003, Philadelphia, PA. pp. 14.

SHORT AND LONG TERM SURVIVAL OF PHYLOGENETICALLY DIVERSE ORGANISMS IN GENEBANKS - (Abstract)

Walters, C., 2003. Short and long term survival of phylogenetically diverse organismsin genebanks. American Association for the Advancement of Science, Febuary 13-18, 2003, Denver, Colorado. p. S21-22.

CONSERVATION OF TROPICAL ISLAND SEEDS: AN EXAMPLE FROM HAWAI'I - (Book/Chapter)

Walters, C. 2003. Conservation of Tropical Island Seeds: an example from Hawai'i. 54:957-963. In R.D. Smith, J.B. Dickic, S.H. Linington, H.W. Pritchard and R.J. Probert (eds) Seed Conservation: turning science into practice. The Royal Botanic Gardens, Kew, London.

TEMPERATURE-INDUCED HELIX/RANDOM COIL TRANSITIONS IN A GROUP 1 LATE EMBRYOGENESIS ABUNDANT PROTEIN FROM SOYBEAN - (Peer Reviewed Journal)

SOULAGES, J.L., KIM, K., WALTERS, C.T., CUSHMAN, J.C. 2002 TEMPERATURE-INDUCED HELIX/RANDOM COIL TRANSITIONS IN A GROUP 1 LATE EMBRYOGENESIS ABUNDANT PROTEIN FROM SOYBEAN. PLANT PHYSIOLOGY. 128: 1-11.

"LIFE AT THE LIMITS: ORGANISMS IN EXTREME ENVIRONMENTS" - (Other)

WALTERS, C.T. "LIFE AT THE LIMITS: ORGANISMS IN EXTREME ENVIRONMENTS". BIOSCIENCE. 2003. v.53.p.524-525

OPTIMIZING SEED BANKING PROCEDURES - (Book/Chapter)

Walters, C. 2003 Optimizing seed banking procedures. 36:723-743. In R.D. Smith, J.B. Dickic, S.H. Linington, H.W. Pritchard and R.J. Probert (eds) Seed Conservation: turning science into practice. The Royal Botanic Gardens, Kew, London.

PRESERVATION OF GENETIC RESOURCES IN THE NATIONAL PLANT GERMPLASM CLONAL COLLECTIONS - (Review Article)

Volk, G.M. and C. Walters. 2003. Preservation of genetic resources in the national plant germplasm clonal collections. Plant Breeding Reviews 23:291-344.

TRIACYLGLYCERIDES DETERMINE THE UNUSUAL STORAGE/PHYSIOLOGY OF CUPHEA SEED - (Peer Reviewed Journal)

Crane, J., A.L. Miller, J.W. van Roekel and C. Walters. 2003. Triacylglycerols determine the unusual storage physiology of Cuphea seed. Planta 217:699-708.

A CRYOPRESERVATION PROTOCOL FOR EMBRYOS OF THE ENDANGERED SPECIES ZIZANIA TEXANA. - (Peer Reviewed Journal)

WALTERS, C.T., TOUCHELL, D.H., POWER, .P., WESLEY, S.J., ANTOLIN, M.F. A CRYOPRESERVATION PROTOCOL FOR EMBRYOS OF THE ENDANGERED SPECIES ZIZANIA TEXANA.. CRYOLETTERS. 2002. 23:291-298

EX SITU CONSERVATION OF PLANT GENETIC RESOURCES - (Proceedings/Symposium)

WALTERS, C. EX SITU CONSERVATION OF PLANT GENETIC RESOURCES. 2002. International Symposium on ART for the Conservation and Genetic Managment of Wildlife. Doorly Zoo, Omaha, NE. Pp 193-195.

VIABILITY LOSSES IN CRYOPRESERVED GERMPLASM:A PILOT STUDY USING LETTUCE SEEDS - (Abstract)

Walters, C., l. Wheeler, and P. Stanwood. 2002. Viability losses in cryopreserved germplasm: A Pilot Study Using Lettuce Seeds. 39th Meeting of the Society for Cryobiology, July 28-31, 2002, Breckenridge, Colorado. p. 78.

MAINTENANCE OF GENETIC VARIABILITY IN A CRYOPRESERVED POPULATION OF ZIZANIA TEXANA EMBRYOS - (Abstract)

Richards, C.M., M. Antolin, and C. Walters. 2002. Maintenance of genetic variability in a cryopreserved population of Zizana texana embryos. 39th Meeting of the Society for Cryobiology, July 28-31, 2002, Breckenridge, Colorado. p. 145.

VEGETATIVELY PROPAGATED COLLECTIONS IN THE U.S. NATIONAL PLANT GERMPLASM SYSTEM. - (Abstract)

Volk, G.M., Walters, C. 2002. Vegetatively propagated collections in the U.S. national plant germplasm system. Cryobiology. 39th Meeting of the Society for Cryobiology, July 28-31, 2002, Breckenridge, Colorado. p.151.

SENSITIVITY OF ARAUCARIA ANGUSTIFOLIA EMBRYOS TO LOW WATER CONTENTS AND TEMPERATURE - (Abstract)

Medeiros, A. and C. Walters. 2002. Sensitivity of Araucaria angustifolia embryos to low water contents and temperature. 7th International Workshop on Seed Biology, May 11-19, 2002, Salamanca, Spain.

CONSERVATION OF GENETIC RESOURCES OF COFFEE USING CRYOPRESERVATION - (Abstract)

Mirian E.TS., C. Walters R.B. Reis, and L.M. Hill. 2002. Conservation of genetic resources of coffee using cryopresvation. 7th International Workshop on Seed Biology, May 11-19, 2002, Salamanca, Spain.

STORABILITY OF PRIMED SUNFLOWER SEEDS AS RELATED TO WATER STATUS - (Abstract)

Bailly, C., B. Poli, F. Corbineau, and C. Walters. 2002. Storability of primed sunflower seeds as related to water status. 7th International Workshop on Seed Biology, May 11-19, 2002, Salamanca, Spain.

CRYOPRESERVATION OF MALUS GERMPLASM: RESULTS USING A WINTER VEGETATIVE BUD METHOD - (Abstract)

TOWILL, L.E., FORSLINE, P., WALTERS, C.T., WADDELL, J.W., LAUFMANN, J. CRYOPRESERVATION OF MALUS GERMPLASM: RESULTS USING A WINTER VEGETATIVE BUD METHOD. XXVIth International Horticultural Congress and Exhibition Program, HORTSCIENCE PROCEEDINGS. Pp. 532. 2002.