Projects

HOST, PATHOGEN AND ENVIRONMENTAL INTERACTIONS IN COOL AND COLD WATER AQUACULTURE Appropriated (D)   Accession Number: 409186

GENOME SEQUENCING AND IDENTIFICATION OF VIRULENCE FACTORS IN FLAVOBACTERIUM PSYCHROPHILUM Nonfunded Cooperative Agreement (N)   Accession Number: 408300

Publications

The genome sequence of Renibacterium salmoninarum suggests reductive evolution away from an environmental Arthrobacter ancestor - (Peer Reviewed Journal)

Wiens, G.D., Rockey, D.D., Wu, Z., Crane, S., Chen, D.S., Capri, G.R., Burnett, J.R., Ponnerassary, S., Schimpma, M.J., Burd, H., Bhattacharyya, A., Rhodes, L.D., Strom, M.S. 2008. The genome sequence of Renibacterium salmoninarum suggests reductive evolution away from an environmental Arthrobacter ancestor. Journal of Bacteriology.

Integrating selective breeding with microbial genomics to improve rainbow trout disease resistance - (Abstract)

Wiens, G.D. 2008. Integrating selective breeding with microbial genomics to improve rainbow trout disease resistance. Meeting Abstract. p. 104.

Challenges associated with heterologous expression of Flavobacterium psychrophilum proteins in Escherichia coli - (Peer Reviewed Journal)

Shah, D.H., Cain, K.D., Wiens, G.D., Call, D.R. 2008. Challenges associated with heterologous expression of Flavobacterium psychrophilum proteins in Escherichia coli. Marine Biotechnology.

Suggestive association of major histocompatibility IB genetic markers with resistance to bacterial cold water disease in rainbow trout (Oncorhynchus mykiss) - (Peer Reviewed Journal)

Johnson, N., Vallejo, R.L., Silverstein, J., Welch, T.J., Wiens, G.D., Hallerman, E.M., Palti, Y. 2008. Suggestive association of major histocompatibility IB genetic markers with resistance to bacterial cold water disease in rainbow trout (Oncorhynchus mykiss). Marine Biotechnology. 10:429-437.

Spleen Size Predicts Resistance of Rainbow Trout to Flavobacterium psychrophilum Challenge - (Peer Reviewed Journal)

Hadidi, S., Glenney, G., Welch, T.J., Silverstein, J., Wiens, G.D. 2008. Spleen Size Predicts Resistance of Rainbow Trout to Flavobacterium psychrophilum Challenge. Journal of Immunology. 180:4156-4165.

PROGRESS TOWARD AN IMMUNOGENETIC MAP FOR RAINBOW TROUT - (Abstract)

Palti, Y., Vallejo, R.L., Wiens, G.D., Rexroad III, C.E. 2008. Progress toward an immunogenetic map for rainbow trout. Plant and Animal Genome Conference.

Recent Advances in Bacterial Coldwater Disease Research - (Trade Journal)

Wiens, G.D., Welch, T.J. 2007. Recent Advances in Bacterial Coldwater Disease Research. Trout Talk Pg 5-6.

PROGRESS TOWARD AN IMMUNOGENETIC MAP FOR RAINBOW TROUT - (Abstract)

Palti, Y., Rexroad III, C.E., Vallejo, R.L., Wiens, G.D. 2007. Progress toward an immunogenetic map for rainbow trout. Meeting Abstract.

Genome Sequence of Flavobacterium psychrophilum Strain CSF 259-93 and Characterization of a Large Cluster of Genes Containing Short Repeats - (Abstract)

Wiens, G.D., Welch, T.J., Rexroad III, C.E., Lapatra, S., Call, D.R., Hunnicutt, D., Bhattacharyya, A., Campbell, J., Walunas, T. 2007. Genome Sequence of Flavobacterium psychrophilum Strain CSF 259-93 and Characterization of a Large Cluster of Genes Containing Short Repeats. Meeting Abstract Pg 12.

Rainbow Trout Innate Immunity against Flavobacterium psychrophilum - (Abstract)

Hadidi, S., Glenney, G.W., Welch, T.J., Silverstein, J., Blazer, V., Wiens, G.D. 2007. Rainbow Trout Innate Immunity against Flavobacterium psychrophilum. Meeting Abstract Pg 35.

PROGRESS TOWARD AN IMMUNOGENETIC MAP FOR RAINBOW TROUT - (Abstract)

Fincham, M.R., Wiens, G.D., Rexroad III, C.E., Vallejo, R.L., Palti, Y. 2007. Progress toward an immunogenetic map for rainbow trout. Meeting Abstract.

Associations of Markers Linked to Major Histocompatibility (MH) Regions With Disease Resistance in Rainbow Trout - (Abstract)

Palti, Y., Johnson, N., Rexroad III, C.E., Welch, T.J., Wiens, G.D., Silverstein, J., Vallejo, R.L. Associations of Markers Linked to Major Histocompatibility (MH) Regions With Disease Resistance in Rainbow Trout. Meeting Abstract.

Early Diversification of the Tumor Necrosis Superfamily in Teleosts: Genomic Characterization and Expression Analysis - (Peer Reviewed Journal)

Glenney, G.W., Wiens, G.D. 2007. Early Diversification of the Tumor Necrosis Superfamily in Teleosts: Genomic Characterization and Expression Analysis. Journal of Immunology, 178:7955-7973.

Spleen Size Predicts Innate Resistance of Rainbow Trout to Flavobacterium psychrophilum Challenge - (Abstract)

Hadidi, S., Glenney, G., Welch, T.J., Silverstein, J., Wiens, G.D. 2007. Spleen Size Predicts Innate Resistance of Rainbow Trout to Flavobacterium psychrophilum Challenge. American Society for Microbiology.

Distribution of Yersinia pestis pIP1202-like Multidrug Resistance Plasmids Among Foodborne Pathogens - (Abstract)

Welch, T.J., Fricke, W.F., Eppinger, M., Rosovitz, M.J., Mcdermott, P., White, D., Wiens, G.D., Ravel, J. 2007. Distribution of Yersinia pestis pIP1202-like Multidrug Resistance Plasmids Among Foodborne Pathogens. American Society of Microbiologists Abstracts.

Identification of potential vaccine target antigens by immunoproteomic analysis of a virulent and a non-virulent strain of the fish pathogen Flavobacterium psychrophilum - (Peer Reviewed Journal)

Sudheesh, P.S., Lafrentz, B.R., Call, D.R., Siems, W.F., Lapatra, S.E., Wiens, G.D., Cain, K.D. 2007. Identification of potential vaccine target antigens by immunoproteomic analysis of a virulent and a non-virulent strain of the fish pathogen Flavobacterium psychrophilum. Diseases of Aquatic Organisms. 74(1):37-47.

Associations of Markers Linked to Major Histocompatibility (MH) Regions and to Other Immune Response Genes with Disease Resistance in Rainbow Trout - (Abstract)

Palti, Y., Johnson, N., Rexroad III, C.E., Welch, T.J., Wiens, G.D., Silverstein, J., Vallejo, R.L. 2006. Associations of Markers Linked to Major Histocompatibility (MH) Regions and to Other Immune Response Genes with Disease Resistance in Rainbow Trout. Plant and Animal Genome Abstracts Pg. 11.

Associations of Markers Linked to Major Histocompatibility (MH) Regions and to Other Immune Response Genes with Disease Resistance in Rainbow Trout - (Abstract)

Palti, Y., Johnson, N., Rexroad III, C.E., Welch, T.J., Wiens, G.D., Silverstein, J., Vallejo, R.L. 2006. Associations of Markers Linked to Major Histocompatibility (MH) Regions and to Other Immune Response Genes with Disease Resistance in Rainbow Trout. World Aquaculture Society Meeting.

CRACKING THE CODE: SELECTING FOR RESISTANCE AGAINST BACTERIAL COLD-WATER DISEASE - (Trade Journal)

Silverstein, J., Palti, Y., Vallejo, R.L., Welch, T.J., Wiens, G.D. 2006. Cracking the code: selecting for resistance against bacterial cold-water disease. Trout Talk.

Application of Microbial Genomics to Improve Aquatic Animal Health - (Abstract)

Wiens, G.D. 2006. Application of Microbial Genomics to Improve Aquatic Animal Health. Meeting Abstract.

SELECTIVE BREEDING AND GENETIC MAPPING OF DISEASE RESISTANCE IN RAINBOW TROUT - (Abstract)

Palti, Y., Rexroad Iii, C.E., Welch, T.J., Wiens, G.D., Silverstein, J., Vallejo, R.L. 2006. Selective breeding and genetic mapping of disease resistance in rainbow trout. Meeting Abstract. Presented at The 9th International Symposium of The International Association for Genetics in Aquaculture, June 26-30, 2006 at Montpellier, France. Meeting Book of Abstracts p. 81.

GENE EXPRESSION AND CELLULAR IMMUNE RESPONSE OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) EXPOSED TO YERSINIA RUCKERI: A MODEL SYSTEM FOR UNDERSTANDING MECHANISMS OF TELEOST PROTECTIVE IMMUNITY - (Abstract)

Wiens, G.D., Lapatra, S., Palti, Y., Rexroad III, C.E., Yao, J., Welch, T.J. 2006. Gene expression and cellular immune response of rainbow trout (Oncorhynchus mykiss) exposed to Yersinia ruckeri: A model system for understanding mechanisms of teleost protective immunity. 10th International Congress, International Society of Developmental and Comparative Immunology, July 1 - 6, 2006. Meeting Abstract. Page 249.

EARLY DIVERSIFICATION OF THE TUMOR NECROSIS SUPERFAMILY IN TELEOSTS: GENOMIC CHARACTERIZATION AND EXPRESSION ANALYSIS - (Abstract)

Glenney, G., Wiens, G.D. 2006. Early diversification of the tumor necrosis superfamily in teleosts: genomic characterization and expression analysis. 10th International Congress, International Society of Developmental and Comparative Immunology. Meeting Abstract Page 113.

BACTERIAL KIDNEY DISEASE - (Book/Chapter)

Wiens, G.D. 2006. Bacterial kidney disease. Aquaculture Compendium. Online at www.cabicompendium.org/ac. Wallingford, UK: CAB International

IDENTIFICATION OF NOVEL RAINBOW TROUT (ONCHORYNCHUS MYKISS) CHEMOKINES, CXCD1 AND CXCD2: MRNA EXPRESSION FOLLOWING YERSINIA RUCKERI VACCINATION AND CHALLENGE - (Peer Reviewed Journal)

Wiens, G.D., Glenney, G.W., Lapatra, S.E., Welch, T.J. 2006. Identification of novel rainbow trout (Onchorynchus mykiss) chemokines, cxcd1 and cxcd2: Mrna expression following Yersinia ruckeri vaccination and challenge. Immunogenetics. 2006 May;58(4):308-23. Epub 2006 Mar 29.

CHARACTERIZATION OF MAJOR HISTOCOMPATIBILITY (MH) HAPLOTYPES AND THEIR ASSOCIATION WITH DISEASE RESISTANCE IN RAINBOW TROUT - (Abstract)

Palti, Y., Rexroad III, C.E., Welch, T.J., Wiens, G.D., Silverstein, J. 2005. Characterization of major histocompatibility (MH) haplotypes and their association with desease resistance in rainbow trout. Plant and Animal Genome Conference. Abstract ID P643, page 262.

ANTIGENIC VARIATION IN RENIBACTERIUM SALMONINARUM P57: FUNCTIONAL AND DIAGNOSTIC IMPLICATIONS - (Abstract)

Wiens, G.D., Ole Bendik, D. 2005. Antigenic variation in Renibacterium salmoninarum p57: functional and diagnostic implications. Bacterial Kidney Disease: Challenges for the 21st Century. Meeting Abstract. November 15-17, 05 Seattle, WA p. 21.

NOVEL VACCINE TARGETS AND ASSESSMENT OF CELLULAR IMMUNITY - (Abstract)

Wiens, G.D. 2005. Novel vaccine targets and assessment of cellular immunity.Workshop on genomics of Renibacterium saloninarum. Meeting Abstract Bacterial Kidney Disease: Challenges for the 21st Century. p. 12.

CONSTRUCTION OF A VIRULENT, GREEN FLUORESCENT PROTEIN-TAGGED YERSINIA RUCKERI AND DETECTION IN TROUT TISSUES AFTER INTRAPERITONEAL AND IMMERSION CHALLENGE - (Peer Reviewed Journal)

Welch, T.J., Wiens, G.D. 2005. Construction of a virulent, green fluorescent protein-tagged yersinia ruckeri and detection in trout tissues after intraperitoneal and immersion challenge. Diseases of Aquatic Organisms. 67:267-272.

IDENTIFICATION AND EXPRESSION OF AN IMMUNO-REACTIVE HEAT SHOCK PROTEIN FROM FLAVOBACTERIUM PSYCHROPHILUM - (Abstract)

Cain, K., Sudheesh, P.S., Lapatra, S.E., Wiens, G.D., Lafrentz, B.R., Call, D.R. 2005. Identification and expression of an immuno-reactive heat shock protein from flavobacterium psychrophilum. Annual Meeting of the Fish Health Section/American Fisheries Society. (abstract).

CHARACTERIZATION OF TOLL-LIKE RECEPTOR 3 GENE IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) - (Peer Reviewed Journal)

Rodriguez, M.F., Wiens, G.D., Purcell, M., Palti, Y. 2005. Characterization of toll-like receptor 3 gene in rainbow trout (oncorhynchus mykiss). Immunogenetics 57(7):510-519. Online pub date 2005 Aug 6, :1-10.

GENOMIC CHARACTERIZATION OF RAINBOW TROUT (ONCORHYNCHUS MYKISS) TOLL-LIKE RECEPTOR GENES - (Abstract)

Rodriguez, M.F., Wiens, G.D., Gahr, S.A., Rexroad III, C.E., Palti, Y. 2005. Genomic characterization of rainbow trout (Oncorhynchus mykiss) toll-like receptor genes. Plant and Animal Genome XIII Abstract W030 page 12.

MAPPING NEUTRALIZING EPITOPES ON RENIBACTERIUM SALMONINARUM P57 USING TRANSPOSON MUTAGENESIS AND SYNTHETIC PEPTIDES - (Peer Reviewed Journal)

Wiens, G.D., Owens, J. 2005. Mapping neutralizing epitopes on renibacterium salmoninarum p57 using transposon mutagenesis and synthetic peptides. Applied and Environmental Microbiology 71 (6): 2894-2901.

GENOME SEQUENCING OF THE VERTICALLY-TRANSMITTED FISH PATHOGEN RENIBACTERIUM SALMONINARUM - (Abstract)

Strom, M., Wiens, G.D., Rockey, D. 2004. Genome sequencing of the vertically-transmitted fish pathogen renibacterium salmoninarum. Meeting Abstract P 90-93.

ESTIMATION OF THE GENOME SIZE OF RENIBACTERIUM SALMONINARUM ATCC 33209 BY PULSED-FIELD GEL ELECTROPHORESIS - (Abstract)

Wiens, G.D., Strom, M.S. 2005. Estimation of the genome size of renibacterium salmoniarum atcc 33209 by pulsed-tield gel electrophoresis. (Abstract). 30th Eastern Fish Health Workshop. p. 8.

GENOMIC CHARACTERIZATION AND EXPRESSION ANALYSIS OF A TUMOR NECROSIS FACTOR SUPERFAMILY 13B (TNFSF 13B) HOMOLOGUE FROM RAINBOW TROUT (ONCORHYNCHUS MYKISS) - (Abstract)

Wiens, G.D., Gahr, S.A., Morrison, C., Palti, Y., Rexroad III, C.E., Rodriguez, M.F., Welch, T.J. 2004. Genomic characterization and expression analysis of a tumor necrosis factor superfamily 13b (tnfsf 13b) homologue from rainbow trout (oncorhynchus mykiss). International Immunology Congress p. 82C (abstract W15.15).

IDENTIFICATION OF TUMOR NECROSIS FACTOR (TNF) AND TNF-RECEPTOR SUPERFAMILY MEMBERS EXPRESSED IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) - (Abstract)

Wiens, G.D. 2004. Identification of tumor necrosis factor (tnf) and tnf-receptor superfamily members expressed in rainbow trout (oncorhynchus mykiss). Annual Eastern Fish Health Workshop p 52.

A PROPOSAL ADVOCATING DRAFT SEQUENCING THE GENOME OF RAINBOW TROUT, ONCORHYNCHUS MYKISS - (Review Article)

Rexroad III, C.E., Palti, Y., Thorgaard, G., Hansen, J., Silverstein, J., Weber, G.M., Wiens, G.D., Gahr, S.A., Rodriguez, M.F., Coulibaly, I., Overturf, K. 2004. A proposal advocating draft sequencing the genome of rainbow trout, oncorhynchus mykiss. Government Publication/Report.. Dept of Energy Joint Genome Institute Community Sequencing Program.

MOLECULAR CHARACTERIZATION OF A PUTATIVE TUMOR NECROSIS FACTOR SUPERFAMILY 13B (TNFSF 13B) HOMOLOGUE FROM RAINBOW TROUT (ONCORHYNCHUS MYKISS) - (Abstract)

Wiens, G.D., Palti, Y., Rexroad III, C.E., Welch, T.J. 2003. Molecular characterization of a putative tumor necrosis factor superfamily 13b (tnfsf 13b) homologue from rainbow trout (oncorhynchus mykiss). Immunology Research Workshop. p.35.

CHARACTERIZATION AND MAPPING OF IMMUNE RESPONSE GENES IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) - (Abstract)

Rodriguez, M.F., Hansen, J., Wiens, G.D., Welch, T.J., Palti, Y. 2003. Characterization and mapping of immune response genes in rainbow trout (oncorhynchus mykiss). Meeting Abstract. Nov 9-15, 2003. p.4.

MAPPING ANTIBODY EPITOPES ON RENIBACTERIUM SALMONINARUM P57 USING TRANSPOSON MUTAGENESIS AND SYNTHETHIC PEPTIDES - (Abstract)

Wiens, G.D, Owen, J., Pascho, R., Winton, J.R. 2003. Mapping antibody epitopes on renibacterium salmoninarum p57 using transposon mutagenesis and synthethic peptides. 28th Annual Eastern Fish Health Workshop. p.70.

REPERTOIRE SHIFT IN THE HUMORAL RESPONSE TO PHOSPHOCHOLINE-KEYHOLE LIMPET HEMOCYANIN: VH SOMATIC MUTATION IN GERMINAL CENTER B CELLS IMPAIRS T15 IG FUNCTION - (Peer Reviewed Journal)

Wiens, G.D., Brown, M., Rittenberg, M.B. 2003. Repertoire shift in the humoral response to phosphocholine-keyhole limpet hemocyanin: vh somatic mutation in germinal center b cells impairs t15 ig function.. Journal of Immunology. 170:5095-5102.