US EPA Manual of Methods for Virology

The electronic text form of the US EPA MANUAL OF METHODS FOR VIROLOGY - EPA publication EPA/600/4-84/013 - is composed of 16 chapters. These chapters are are presented here in Acrobat PDF format. You will need Adobe Acrobat Reader to view PDF files. See EPA's PDF page for more information about getting and using the free Acrobat Reader.

Chapter 1. Introduction (PDF, 10 pp., 45 KB)
Chapter 2. Cleansing Laboratory Ware and Equipment (PDF, 24pp., 219 KB)
Chapter 3. Sterilization and Disinfection (PDF, 5 pp., 90 KB)
Chapter 4. Quality Assurance (PDF, 19 pp., 108 KB)
Chapter 5. Virus Adsorption-Elution (Viradel) Disc Filter Procedures for Recovering Viruses from Sewages, Effluents, and Waters (PDF, 30 pp., 1 MB)
Chapter 6. Virus Adsorption-Elution (Viradel) Cartridge Filter Procedures for Recovering Viruses from Sewage, Effluents, and Waters (PDF, 46 pp., 1.3 MB)
Chapter 7. Method for Recovering Viruses from Sludges (and Other Solids) (PDF, 16 pp., 263 KB)
Chapter 8. Method for Reduction of Cytotoxicity of Sample Concentrates (PDF, 9 pp., 48 KB)
Chapter 9. Cell Culture Preparation and Maintenance (PDF, 21 pp., 11 KB)
Chapter 10. Cell Culture Procedures for Assaying Plaque-Forming Viruses (PDF, 28 pp., 263 KB)
Chapter 11. Virus Plaque Confirmation Procedures (PDF, 6 pp., 34 KB)
Chapter 12. Identification of Enteroviruses (PDF, 10 pp., 137 KB)
Chapter 13. Data Analysis and Experimental Design (not yet available in PDF format)
Chapter 14. Concentration and Processing of Waterborne Viruses by Positive Charge 1MDS Cartridge Filters and Organic Flocculation (PDF, 12 pp., 1.1 MB)
Chapter 15. Total Culturable Virus Quantal Assay (PDF, 11 pp., 1 MB)
Chapter 16. Procedures for Detecting Coliphages (PDF, 10 pp., 593 KB)

This Manual is also available in hardcopy form. The hardcopy form of the Manual can be obtained by contacting:

Branch Chief, Biohazard Assessment Research Branch
Microbiological and Chemical Exposure Assessment Research Division
National Exposure Research Laboratory
U.S. Environmental Protection Agency
26 West M.L. King Dr.
Cincinnati OH 45268 USA

e-mail: ci_nerl_mceard@epa.gov

Original Date of Publication: February 1984
Subsequent Chapter Revision/Addition Dates: 1986, 1987, 1988, 1989, 1991, 1993 , 2001

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US EPA Manual of Methods for Virology

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