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Appendix Figure. Simultaneous array detection of biological warfare
agents in mixed autoclaved bacterial culture samples amplified with primer
pool I (A), primer pool II (B), and wastewater samples spiked with primer
pool I (C). Mixed samples were prepared by mixing Bacillus anthracis
with B. thuringiensis kurstaki at 1:1, 1:5 and 1:9 ratios and Yersinia
pestis with Francisella tularensis at 1:1 and 1:9 ratios. Wastewater
samples were prepared by spiking individual autoclaved bacterial cultures
of B. anthracis, B. thuringiensis kurstaki, Y. pestis,
F. tularensis, Clostridium botulinum, and vaccinia virus
at 1:10 dilution. The standard deviation (SD) of background images is
15 (n = 3), and the detection limit is 45, defined as 3 × SD.
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