Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Snapper  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Ocyurus chrysurus
FDA Seafood List Common Name: Yellowtail Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19970207gel06
Unique Web Code: ysh04Srr
Tissue Aliquot Code: ocyuchry H94-003 #08m Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55829.90.049560250.4022125.24.14.5582.34.70.0482524
25.217721.30.075988850.6537768.46.65.2177.650.0736684
35.8528934.70.13581851.2271615.812.55.85289.350.138164
46.5542751.30.12172581.5418119.815.76.55426.67.60.114177
56.8546055.30.037930960.3796994.93.96.85460.26.10.0351348
67.3552663.20.061597960.6769938.76.97.35525.96.30.0603736
78.1565178.20.098900471.2382515.912.68.15650.97.30.0960845
88.4571786.20.087334271.057613.610.78.45715.96.80.0875483
98.6576591.90.066533150.5548487.15.68.65764.74.80.0649193
109.381497.80.007449330.0375050.50.49.3813.630.0070652
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.169273.20.11417991.742844.744.16927.88.70.113139
24.588810.60.017989780.2516420.70.64.5887.28.30.0171564
34.81612314.80.25183662.615767.16.14.816122.960.24621
44.95814417.30.0487903121.061262.92.54.95814011.70.0512543
55.13917120.60.00919140.06269910.20.15.1391724.40.008055
65.40421525.80.048871560.4622841.31.15.404214.66.10.042465
75.62625330.40.020727850.1805310.50.45.626253.74.90.0206284
85.84129034.90.043663240.306210.80.75.841290.740.0429799
95.98131738.10.53943754.410751210.25.981317.24.70.534127
106.07433540.30.32378762.9489186.86.074334.55.50.300393
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.15135042.10.19385451.683244.63.96.1513505.20.182122
126.19735943.10.19112441.272343.52.96.197359.14.20.170714
136.31638245.90.01564520.05822340.20.16.316382.420.0161278
146.491416500.11841351.192663.22.86.491415.55.50.122899
156.55542851.40.054449210.06628750.20.26.555427.510.0367931
166.62743652.40.44825521.946025.34.56.6274362.40.449684
176.70944553.50.15885441.12743.12.66.709444.540.159144
186.81845754.90.22190672.421246.65.66.818457.96.70.202919
196.93747256.70.038423210.02263120.10.16.9374721.20.0102698
207.09649359.30.46938776.2177316.914.47.0964927.40.471238
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.2150861.10.025532810007.2150310
228.56874589.50.12317690.6813811.81.68.568740.48.70.0441446
238.71677092.50.151141246.1471816.714.28.716767.4240.14451
PeakCounter: 23
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.558510.20.047564750.4207624.73.84.5585.35.10.0470003
25.2183220.060355660.7059187.86.35.2182.66.40.0623224
35.8529435.30.13695451.2796914.211.55.85294.15.20.138038
46.5542851.40.10521981.4534516.2136.55426.78.40.0979535
56.8546155.40.037634470.4449844.946.85462.67.20.0347823
67.3552763.30.055539260.6034996.75.47.35526.66.30.0541493
78.1565378.50.10346371.2825814.311.58.15652.37.20.101185
88.4571686.10.098411971.1995713.310.78.45715.770.0960787
98.6576591.90.06810950.5634036.358.65764.34.80.0663617
109.3815980.0643663101.0397111.69.39.3811.410.10.058123
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.155273.20.078338291.1819743.74.155278.50.0782678
24.558610.30.02919660.29225610.94.5586.75.80.0281987
34.82512715.30.17598972.166837.46.84.825127.17.20.17043
44.96514817.80.0669675121.4592154.54.96514612.20.0672663
55.2183220.034649980.4822021.61.55.2179.98.20.0333726
65.38721525.80.0152961130.347581.21.15.387211.213.30.0146933
75.62725630.80.008460260.09408080.30.35.627257.56.30.008453
85.8529435.30.077472360.7757782.62.45.85294.160.0727373
95.97531838.20.4584143.2413711.110.15.975318.440.451944
106.06933640.40.22802852.007396.86.36.069335.95.30.212378
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.15235242.30.15066351.228414.23.86.152352.24.70.146116
126.19936143.40.15001741.099383.83.46.199361.14.40.142391
136.32438546.30.008856720.03606770.10.16.324385.72.20.0092265
146.51242150.60.084402160.9039683.12.86.512420.25.80.087203
156.58343251.90.027040730.1689730.60.56.583434.62.70.0346858
166.67444253.10.34105231.814186.25.76.674441.330.342736
176.74745054.10.14784351.274064.346.747450.14.90.148166
186.85546255.50.19527251.714425.95.36.855461.85.10.188935
196.96947757.30.033615970.4776961.61.56.969471.66.70.0400734
207.12949859.90.45185575.771819.7187.129496.27.20.451763
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.23651261.50.021486310007.23650810
227.3552763.30.019184950.1825710.60.67.35525.84.90.0209235
238.1565178.20.04568690.6522492.228.15649.69.10.0403053
248.4572687.30.026182450.2406370.80.88.457255.50.0248709
258.53474589.50.0195244110.4346081.51.48.534751.110.80.0226685
268.6577192.70.0625329121.257684.33.98.65773.112.20.0579386
PeakCounter: 26
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.154283.40.12311291.896256.25.54.15428.88.80.121942
24.82412715.30.21243472.561818.47.44.824127.76.80.211898
34.96614817.80.0764074101.339554.43.94.966145.89.90.0765884
45.35620925.10.0292109100.4901531.61.45.356210.29.60.0287056
55.63825730.90.019463450.1923420.60.65.638256.15.50.0197414
65.82128834.60.044746660.4093571.31.25.821288.35.60.0415376
75.96431537.90.50036954.3306814.212.55.964315.14.90.496236
86.056333400.27046462.543238.37.36.0563335.70.252371
96.14935142.20.1766151.391844.646.149350.54.60.170617
106.19636043.30.18410141.237994.13.66.196359.73.90.177846
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.32538546.30.017382420.075330.20.26.325384.72.40.0179092
126.541950.40.10991251.03513.436.5418.95.10.114717
136.57143151.80.036140710.05961820.20.26.5714311.30.0257937
146.65344052.90.42976321.9036.25.56.653440.22.50.431466
156.735449540.16996541.057963.53.16.735448.63.70.163202
166.84446155.40.23925672.768989.186.844461.47.40.211359
176.9747757.30.028445510006.9743510
187.11949659.60.49028476.4815121.218.77.119494.87.50.489331
197.23651161.40.023762610007.23650710
208.55874689.70.0230847110.4136121.41.28.558742.310.50.0221683
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.71677292.80.024478380.3473831.118.716774.28.30.0237522
PeakCounter: 21
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.558710.50.047715550.3964314.63.64.5587.34.80.0462827
25.218221.90.081644550.7665858.86.95.2181.75.50.0789833
35.8529235.10.13652451.2681814.611.45.85291.75.30.135713
46.5542951.60.11463381.5038517.313.56.55428.67.80.10815
56.8546255.50.036429860.3772294.33.46.85461.86.10.0349515
67.35524630.067001560.7229228.36.57.35524.860.0674501
78.1565378.50.11793271.5272717.613.78.15653.17.50.114907
88.4571786.20.12892461.384831612.48.45716.76.40.122643
98.6576391.70.070566350.6511717.55.88.657635.30.0695417
109.381798.20.013621630.08046380.90.79.3816.83.10.014868
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 6.85 label for gel schematic
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pI 8.15 label for gel schematic
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pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 100
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 23 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 26 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 21 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln