RFE Fish Species Identification Isoelectric Focusing Tools - Yellowtail Snapper PAGPlate IEF Gel 01

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Ocyurus chrysurus
FDA Seafood List Common Name: Yellowtail Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19940901gel07
Unique Web Code: ysg01Srr
Tissue Aliquot Code: ocyuchry H94-002 #02a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Specimen	Lane e Co-Migrated	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 18
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 20
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	57	5	0.233129	10	3.88186	9.6	7.4	3.5	58.3	9.7	0.225363
2	4.55	215	19	0.354783	6	3.07624	7.6	5.8	4.55	214.8	5.7	0.301906
3	5.2	295	26.1	0.485852	5	4.34224	10.8	8.2	5.2	295.3	5.1	0.478763
4	5.85	437	38.7	0.720354	3	4.13571	10.2	7.9	5.85	436.7	3.2	0.731892
5	6.55	608	53.9	0.570577	4	3.77188	9.3	7.2	6.55	607.6	3.8	0.556155
6	6.85	645	57.1	0.215598	5	1.69608	4.2	3.2	6.85	645.1	5.1	0.187838
7	7.35	734	65	0.627652	4	4.94862	12.3	9.4	7.35	733.7	4.5	0.626892
8	8.15	886	78.5	0.115569	8	1.67034	4.1	3.2	8.15	885.9	8.4	0.112571
9	8.45	957	84.8	0.146387	6	1.47661	3.7	2.8	8.45	957.1	5.7	0.144936
10	8.65	999	88.5	0.141848	5	1.15959	2.9	2.2	8.65	999.1	4.7	0.139996
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1107	98.1	0.669979	9	10.202	25.3	19.4	9.3	1106.3	9.3	0.620474

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 17
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 19
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.682	88	7.6	0.0685835	9	1.09362	1.5	1.4	3.682	90.9	8.9	0.0696137
2	4.093	150	13	0.788136	5	7.13099	9.9	8.9	4.093	151	5	0.799729
3	4.431	201	17.4	0.0209107	5	0.254712	0.4	0.3	4.431	203.7	4.5	0.0318089
4	4.504	212	18.3	0.0763787	3	0.457632	0.6	0.6	4.504	211.8	3.4	0.0748868
5	4.662	233	20.2	0.0809187	8	1.02272	1.4	1.3	4.662	233.5	7.6	0.0757042
6	4.743	243	21	0.0570578	4	0.298386	0.4	0.4	4.743	244	3.6	0.0462004
7	4.903	263	22.8	0.0342341	4	0.229905	0.3	0.3	4.903	261.9	3.8	0.0342079
8	5.04	280	24.2	0.429819	10	7.38604	10.3	9.3	5.04	281.9	9.6	0.434953
9	5.176	297	25.7	0.5622	5	5.03177	7	6.3	5.176	296.8	5.5	0.516966
10	5.361	335	29	0.105253	7	1.11774	1.6	1.4	5.361	334.1	6.5	0.0968432
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.48	361	31.2	0.237464	3	1.15856	1.6	1.5	5.48	361.1	3.1	0.21031
12	5.549	376	32.5	0.0278059	11	0.748723	1	0.9	5.549	368.2	10.6	0.0399585
13	5.641	396	34.3	0.0508349	4	0.371541	0.5	0.5	5.641	395.7	4.2	0.0501125
14	5.77	424	36.7	0.0753024	5	0.6179	0.9	0.8	5.77	423.1	4.7	0.0735188
15	5.82	435	37.6	0.196861	3	1.10701	1.5	1.4	5.82	435	3.4	0.185282
16	5.888	451	39	0.0976417	7	1.26595	1.8	1.6	5.888	452.6	7.5	0.0956803
17	5.976	473	40.9	0.728006	5	5.96887	8.3	7.5	5.976	473.4	4.7	0.721611
18	6.052	492	42.6	0.761943	5	5.3968	7.5	6.8	6.052	491.5	4.5	0.675556
19	6.136	513	44.4	0.407516	6	3.89881	5.4	4.9	6.136	511.8	6.3	0.349201
20	6.2	529	45.8	0.0700302	7	1.01381	1.4	1.3	6.2	526.7	6.9	0.0823323
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.324	560	48.4	0.0230231	7	0.209542	0.3	0.3	6.324	558	7	0.0169977
22	6.364	570	49.3	0.0205035	2	0.0797158	0.1	0.1	6.364	570.3	2.5	0.0181965
23	6.452	592	51.2	0.016364	2	0.0687454	0.1	0.1	6.452	591.7	2.5	0.0157897
24	6.496	603	52.2	0.0629365	7	0.620308	0.9	0.8	6.496	605.4	7.1	0.04955
25	6.54	614	53.1	0.131396	1	0.281405	0.4	0.4	6.54	613.4	1.4	0.11466
26	6.595	622	53.8	0.666025	2	2.13777	3	2.7	6.595	622.2	1.8	0.684368
27	6.716	637	55.1	0.308004	4	2.14576	3	2.7	6.716	637.1	4.1	0.294383
28	6.822	650	56.2	0.234046	2	1.00584	1.4	1.3	6.822	650.3	2.5	0.230626
29	6.903	663	57.4	0.0989043	6	0.896705	1.2	1.1	6.903	663.7	5.9	0.0861941
30	6.964	674	58.3	0.074094	3	0.440006	0.6	0.6	6.964	675.1	3.4	0.073557
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.069	693	59.9	0.756832	4	5.28609	7.3	6.6	7.069	693.6	3.9	0.768695
32	7.136	705	61	0.503654	3	3.07766	4.3	3.9	7.136	704.9	3.4	0.504604
33	7.197	716	61.9	0.0667487	8	0.826821	1.1	1	7.197	715.8	7.7	0.0607447
34	7.547	781	67.6	0.0413115	5	0.338561	0.5	0.4	7.547	779.9	4.5	0.0423354
35	8.513	976	84.4	0.132728	18	4.80246	6.7	6	8.513	983.4	18	0.150531
36	8.628	1000	86.5	0.375032	8	4.17916	5.8	5.2	8.628	1001.6	7.9	0.297824

PeakCounter: 36

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	59	5.2	0.246041	24	8.45337	18.9	16.2	3.5	52.5	23.7	0.20121
2	4.55	218	19.1	0.290389	7	3.33157	7.5	6.4	4.55	217.2	7.1	0.265775
3	5.2	300	26.2	0.405144	7	4.72084	10.6	9	5.2	299.2	6.6	0.403074
4	5.85	441	38.6	0.650748	4	4.66339	10.4	8.9	5.85	440.9	4.1	0.649523
5	6.55	620	54.2	0.56495	4	3.96593	8.9	7.6	6.55	619.4	4	0.554929
6	6.85	657	57.5	0.216031	6	1.86616	4.2	3.6	6.85	656.7	5.7	0.18551
7	7.35	748	65.4	0.63527	5	5.1576	11.5	9.9	7.35	747.4	4.6	0.631024
8	8.15	901	78.8	0.108372	8	1.47866	3.3	2.8	8.15	900.1	7.7	0.10827
9	8.45	964	84.3	0.133517	6	1.36545	3.1	2.6	8.45	964	5.9	0.131018
10	8.65	1005	87.9	0.126929	5	1.04005	2.3	2	8.65	1004.2	4.7	0.124517
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1102	96.4	0.626087	8	8.66746	19.4	16.6	9.3	1102	8.2	0.5947

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.732	99	8.7	0.050253	10	0.903614	1.2	1	3.732	97.8	10.2	0.0501371
2	4.046	147	12.8	0.767064	5	6.77402	9.1	7.8	4.046	148.1	4.9	0.7722
3	4.432	206	18	0.0905945	8	1.01989	1.4	1.2	4.432	204.7	8.5	0.067766
4	4.574	227	19.8	0.0923064	7	1.10054	1.5	1.3	4.574	226.8	7.3	0.0850481
5	4.662	238	20.8	0.0716913	4	0.402778	0.5	0.5	4.662	237.7	3.7	0.061951
6	4.941	273	23.9	0.391114	12	8.02588	10.8	9.2	4.941	273.6	11.5	0.393695
7	5.076	290	25.3	0.59596	5	4.63102	6.2	5.3	5.076	290	4.9	0.528919
8	5.313	330	28.8	0.155559	7	1.5683	2.1	1.8	5.313	327.6	7.1	0.125348
9	5.428	355	31	0.273171	3	1.43202	1.9	1.6	5.428	354.9	3.1	0.261558
10	5.594	391	34.2	0.0564087	4	0.369403	0.5	0.4	5.594	391.2	3.7	0.0564369
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.686	411	35.9	0.0613617	7	0.765653	1	0.9	5.686	414.7	7.2	0.0599151
12	5.774	430	37.6	0.253489	4	1.62472	2.2	1.9	5.774	430.2	3.8	0.240533
13	5.868	451	39.4	0.100071	5	0.970524	1.3	1.1	5.868	450.3	5.1	0.107271
14	5.938	468	40.9	0.811457	5	6.17066	8.3	7.1	5.938	468.8	4.5	0.767971
15	6.028	490	42.8	0.758328	5	5.66844	7.6	6.5	6.028	489.9	4.7	0.676516
16	6.122	513	44.8	0.332922	9	4.31752	5.8	4.9	6.122	511.2	8.8	0.275937
17	6.368	573	50.1	0.0380098	2	0.151549	0.2	0.2	6.368	573.1	2.4	0.0356461
18	6.471	598	52.3	0.0961923	4	0.633164	0.9	0.7	6.471	597.8	3.9	0.0926724
19	6.532	613	53.6	0.175265	3	0.767659	1	0.9	6.532	613.2	2.7	0.158703
20	6.588	622	54.4	0.744893	2	2.24602	3	2.6	6.588	622	1.7	0.741912
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.74	641	56	0.27448	4	1.79627	2.4	2.1	6.74	640.8	3.8	0.266299
22	6.821	651	56.9	0.182678	3	0.843754	1.1	1	6.821	651.4	2.5	0.189393
23	6.896	663	58	0.0966571	3	0.554082	0.7	0.6	6.896	663.8	3.3	0.0943835
24	6.978	678	59.3	0.0735795	3	0.367839	0.5	0.4	6.978	678.2	2.8	0.0748294
25	7.056	692	60.5	0.736648	3	4.07513	5.5	4.7	7.056	692.5	3.1	0.746853
26	7.094	699	61.1	0.424334	4	2.85449	3.8	3.3	7.094	699.7	3.6	0.442033
27	7.138	707	61.8	0.602713	3	2.59486	3.5	3	7.138	707	2.5	0.585125
28	7.226	723	63.2	0.0472381	5	0.38434	0.5	0.4	7.226	721.9	4.8	0.0450874
29	7.564	787	68.8	0.0382324	5	0.31962	0.4	0.4	7.564	785.7	4.9	0.0366347
30	8.49	970	84.8	0.217361	18	6.61744	8.9	7.6	8.49	974.8	18	0.20742
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.624	999	87.3	0.408424	9	4.45879	6	5.1	8.624	999.9	9.2	0.272421

PeakCounter: 31

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	68	5.9	0.188913	6	1.8353	2.3	2.1	3.5	67.3	6.4	0.163066
2	3.584	81	7.1	0.141002	10	2.38002	2.9	2.8	3.584	80	10	0.134935
3	4.025	149	13	0.738999	4	5.95305	7.4	6.9	4.025	149.5	4.4	0.765778
4	4.427	211	18.4	0.0933536	8	1.13206	1.4	1.3	4.427	210.4	8.2	0.0776974
5	4.55	230	20.1	0.236803	7	2.69008	3.3	3.1	4.55	229.1	6.6	0.23132
6	4.654	243	21.2	0.062512	5	0.582462	0.7	0.7	4.654	241.5	5.1	0.0646704
7	4.807	262	22.9	0.0427867	7	0.474745	0.6	0.5	4.807	262.6	7.3	0.036922
8	4.951	280	24.5	0.0575329	4	0.339377	0.4	0.4	4.951	278.9	3.5	0.0541864
9	5.088	297	25.9	0.700771	5	5.58343	6.9	6.5	5.088	296.7	4.5	0.695046
10	5.2	311	27.2	0.25789	6	2.68075	3.3	3.1	5.2	310.9	5.9	0.257154
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.306	334	29.2	0.0674937	7	0.773519	1	0.9	5.306	332.2	6.9	0.0635766
12	5.403	355	31	0.139596	4	0.959354	1.2	1.1	5.403	355.1	4	0.134785
13	5.573	392	34.2	0.035468	3	0.234064	0.3	0.3	5.573	393.3	3.1	0.0421127
14	5.684	416	36.3	0.100349	5	0.783115	1	0.9	5.684	415.7	4.5	0.0977383
15	5.758	432	37.7	0.194594	4	1.42714	1.8	1.6	5.758	432	4.4	0.18294
16	5.85	452	39.5	0.45978	3	2.56508	3.2	3	5.85	452.3	3.1	0.464729
17	5.923	469	41	0.792396	4	5.81201	7.2	6.7	5.923	469.9	4.2	0.782863
18	6.026	493	43.1	0.670377	4	4.31367	5.3	5	6.026	492.7	3.8	0.640552
19	6.121	515	45	0.177236	6	1.73778	2.1	2	6.121	514	5.9	0.166682
20	6.384	576	50.3	0.0241411	5	0.17639	0.2	0.2	6.384	576.5	4.9	0.0201419
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.487	600	52.4	0.0898566	11	1.50769	1.9	1.7	6.487	604	10.7	0.0797431
22	6.543	613	53.5	0.29748	4	1.97019	2.4	2.3	6.543	613.7	3.9	0.283323
23	6.632	625	54.6	0.731489	2	1.92362	2.4	2.2	6.632	625	1.5	0.723463
24	6.72	636	55.5	0.0776125	2	0.265885	0.3	0.3	6.72	636.1	2.4	0.0633677
25	6.815	648	56.6	0.220383	8	3.07963	3.8	3.6	6.815	649.7	8.2	0.211814
26	6.936	668	58.3	0.0760067	2	0.247894	0.3	0.3	6.936	668.6	2.4	0.0580604
27	7.036	686	59.9	0.105228	4	0.625653	0.8	0.7	7.036	686.1	3.8	0.0922326
28	7.102	698	61	0.720928	4	4.87752	6	5.6	7.102	698.3	4.1	0.672295
29	7.174	711	62.1	0.587065	5	4.36339	5.4	5	7.174	709.4	4.8	0.508015
30	7.267	728	63.6	0.0563297	9	0.883753	1.1	1	7.267	733.1	8.5	0.0585809
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.35	743	64.9	0.514367	4	3.58787	4.4	4.1	7.35	742.3	4.1	0.499609
32	7.613	795	69.4	0.0265558	6	0.291926	0.4	0.3	7.613	792.9	6.3	0.0260836
33	8.15	901	78.7	0.233126	5	2.03156	2.5	2.3	8.15	902.8	5.3	0.214561
34	8.45	959	83.8	0.11974	15	2.61552	3.2	3	8.45	958.6	14.6	0.10094
35	8.53	977	85.3	0.0393113	4	0.150141	0.2	0.2	8.53	978	4.2	0.0203856
36	8.65	1004	87.7	0.214651	8	2.7727	3.4	3.2	8.65	1004	7.6	0.206928
37	9.3	1115	97.4	0.500371	9	7.21116	8.9	8.3	9.3	1113.7	8.6	0.470507

PeakCounter: 37

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	66	5.8	0.240201	14	5.91311	15.3	12	3.5	70.6	14.5	0.230344
2	4.55	209	18.3	0.301055	6	2.87446	7.4	5.9	4.55	209.7	6.1	0.265592
3	5.2	289	25.2	0.392543	6	3.84589	10	7.8	5.2	288.4	5.7	0.383364
4	5.85	441	38.5	0.64799	3	3.8851	10.1	7.9	5.85	440.6	3.3	0.669325
5	6.55	611	53.4	0.476506	4	3.56496	9.2	7.3	6.55	610.5	4.4	0.455642
6	6.85	649	56.7	0.202455	6	2.0572	5.3	4.2	6.85	649.2	6.5	0.179801
7	7.35	742	64.8	0.572613	5	4.98156	12.9	10.2	7.35	741.2	5	0.562053
8	8.15	901	78.7	0.112468	9	1.6086	4.2	3.3	8.15	900.6	8.5	0.106324
9	8.45	964	84.2	0.148517	6	1.62969	4.2	3.3	8.45	963.5	6.3	0.145035
10	8.65	1005	87.8	0.128059	5	1.14993	3	2.3	8.65	1004.5	5.2	0.125055
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1124	98.2	0.577677	8	7.10286	18.4	14.5	9.3	1122.1	7.7	0.517915

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK SP CO MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 137

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 9.600 4.550 7.600 5.200 10.800 5.850 10.200 6.550 9.300 6.850 4.200 7.350 12.300 8.150 4.100 8.450 3.700 8.650 2.900 9.300 25.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 18 LANE TYPE: pI Markers ACTUAL GEL LANE: 20 PeakCount: 11 Return to Lane Navigator
3.682 1.500 4.093 9.900 4.431 0.400 4.504 0.600 4.662 1.400 4.743 0.400 4.903 0.300 5.040 10.300 5.176 7.000 5.361 1.600 5.480 1.600 5.549 1.000 5.641 0.500 5.770 0.900 5.820 1.500 5.888 1.800 5.976 8.300 6.052 7.500 6.136 5.400 6.200 1.400 6.324 0.300 6.364 0.100 6.452 0.100 6.496 0.900 6.540 0.400 6.595 3.000 6.716 3.000 6.822 1.400 6.903 1.200 6.964 0.600 7.069 7.300 7.136 4.300 7.197 1.100 7.547 0.500 8.513 6.700 8.628 5.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 17 LANE TYPE: Specimen ACTUAL GEL LANE: 19 PeakCount: 36 Return to Lane Navigator
3.500 18.900 4.550 7.500 5.200 10.600 5.850 10.400 6.550 8.900 6.850 4.200 7.350 11.500 8.150 3.300 8.450 3.100 8.650 2.300 9.300 19.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator
3.732 1.200 4.046 9.100 4.432 1.400 4.574 1.500 4.662 0.500 4.941 10.800 5.076 6.200 5.313 2.100 5.428 1.900 5.594 0.500 5.686 1.000 5.774 2.200 5.868 1.300 5.938 8.300 6.028 7.600 6.122 5.800 6.368 0.200 6.471 0.900 6.532 1.000 6.588 3.000 6.740 2.400 6.821 1.100 6.896 0.700 6.978 0.500 7.056 5.500 7.094 3.800 7.138 3.500 7.226 0.500 7.564 0.400 8.490 8.900 8.624 6.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 31 Return to Lane Navigator
3.500 2.300 3.584 2.900 4.025 7.400 4.427 1.400 4.550 3.300 4.654 0.700 4.807 0.600 4.951 0.400 5.088 6.900 5.200 3.300 5.306 1.000 5.403 1.200 5.573 0.300 5.684 1.000 5.758 1.800 5.850 3.200 5.923 7.200 6.026 5.300 6.121 2.100 6.384 0.200 6.487 1.900 6.543 2.400 6.632 2.400 6.720 0.300 6.815 3.800 6.936 0.300 7.036 0.800 7.102 6.000 7.174 5.400 7.267 1.100 7.350 4.400 7.613 0.400 8.150 2.500 8.450 3.200 8.530 0.200 8.650 3.400 9.300 8.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 37 Return to Lane Navigator
3.500 15.300 4.550 7.400 5.200 10.000 5.850 10.100 6.550 9.200 6.850 5.300 7.350 12.900 8.150 4.200 8.450 4.200 8.650 3.000 9.300 18.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Yellowtail Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Snapper ---- Go to Display Gel Lane Navigator.