Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowfin Tuna  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Thunnus albacares
FDA Seafood List Common Name: Yellowfin Tuna
FDA Seafood List Market Name: Tuna
Unique IEF Gel Code: 19970430gel04
Unique Web Code: yng02Srr
Tissue Aliquot Code: thunalba J96-001 #02a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 11
LANE TYPE: pI Markers
ACTUAL GEL LANE: 13
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5723.20.3169193418.674521.7173.581.3340.31016
24.5536916.70.250936125.054365.94.64.5537012.20.233935
35.254524.60.420188107.537658.86.95.254510.40.410628
45.8587039.30.79410379.700111.38.85.85869.66.60.8271
56.55120354.30.483807107.7496997.16.551201.69.80.444192
66.85129158.30.448404118.3729.77.66.851289.911.30.417444
77.35147866.70.5955851111.609713.510.67.351475.811.40.576279
88.15177680.10.110693183.4269543.18.151776.2180.107437
98.45188985.20.19766134.304453.98.45188912.70.191959
108.65195888.40.17018102.968833.52.78.651957.6100.167678
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3217798.20.356276116.605797.769.32173.911.50.324224
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 12
LANE TYPE: Specimen
ACTUAL GEL LANE: 14
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.76142819.30.1393871.690621.91.34.761427.27.20.132438
25.24557025.70.16861164.393284.83.35.245568.915.60.159067
35.61752340.15083181.897792.11.45.61752.17.50.142256
45.66277835.10.090542591.405451.515.662778.28.80.0903072
56.221104847.30.1742841.263811.40.96.22110484.20.171319
66.398113251.10.11232581.657531.81.26.3981132.68.30.113354
76.555120654.40.76965168.532759.46.46.5551205.96.20.77585
86.694124656.30.473893149.98963117.56.6941248.813.70.412025
96.781127157.40.72739666.188636.84.66.78112715.60.628133
106.973133760.40.27366194.201374.63.16.9731339.19.40.252701
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
117.099138462.50.26009783.124993.42.37.09913867.60.232747
127.18141463.80.80552579.4167410.477.181415.56.60.802618
137.236143564.80.173341102.790553.12.17.236143210.10.155477
147.499153369.20.26979994.5458253.47.4991530.49.10.281026
157.6061573710.73682789.766710.87.37.6061571.97.50.733556
168.4351883850.214586103.839754.22.98.4351884.79.80.221839
178.998207593.70.3444372816.021317.7128.9982075.128.50.317238
PeakCounter: 17
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 13
LANE TYPE: pI Markers
ACTUAL GEL LANE: 15
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5733.30.3609813219.17692116.23.578.8320.338512
24.5537216.80.288468125.815346.44.94.55373.612.40.264084
35.255024.90.430771118.2114496.95.2550.910.90.424752
45.8587339.40.75018479.5859510.58.15.85872.47.10.765019
56.55120654.50.5184297.980088.76.76.551205.19.20.488864
66.85129158.30.468087118.270249.176.851290.910.70.437528
77.35147766.70.5704951110.622811.68.97.351474.6110.547093
88.15177580.20.125922194.090964.53.48.151773.218.90.121821
98.45188885.30.21409124.446534.93.78.451887.111.80.211783
108.65195688.40.203469103.561533.938.651955.6100.200199
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3217898.40.509578119.5645510.58.19.32175.611.40.475384
PeakCounter: 11
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 14
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 16
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5652.90.3165882312.1617.36.23.568.223.20.295435
24.5538117.30.218477155.458693.32.84.55383.914.70.209744
34.6641118.60.1580361.543170.90.84.66410.560.145722
45.13454124.50.150481102.097211.31.15.134539.79.80.120382
55.255925.30.362841117.308654.43.75.2559.111.50.35872
65.56973533.30.1335161.272830.80.65.569735.65.60.127118
75.62876334.60.078396181.080730.60.55.628761.78.30.0738814
85.8586939.40.67640667.530344.53.85.85869.36.20.688555
96.204104147.20.11595940.8991860.50.56.20410414.40.115208
106.389113151.30.129159101.964921.216.38911319.90.112032
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.521119554.20.257581115.0638932.66.521119711.50.248828
126.55120954.80.69080255.215053.12.66.551209.54.80.615863
136.739125356.80.431285106.839384.13.56.7391255.210.40.37128
146.851279580.59880287.309754.43.76.851279.17.60.541191
157.04134961.20.18667103.3315321.77.041349.6100.187173
167.165139563.20.13971671.6148610.87.1651396.76.60.138613
177.252142764.70.60824188.178984.94.27.2521426.27.50.611537
187.309144865.60.10928371.292250.80.77.309144870.103722
197.35146366.30.3627874.763292.82.47.351463.47.30.367279
207.543153569.60.24169693.89282.327.5431534.890.244973
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.655157771.50.67529289.321165.64.77.6551576.67.80.671352
228.15176279.90.176831206.174683.73.18.151761.920.30.17158
238.45189485.90.3766172112.01597.26.18.451894.420.50.330424
248.65195888.80.426183626.72131613.68.651956.6360.418782
258.978206793.70.3196992714.0398.47.18.9782066.826.80.295644
269.3217498.50.530171210.40316.25.39.32170.811.60.507892
PeakCounter: 26
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 15
LANE TYPE: Specimen
ACTUAL GEL LANE: 17
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.7420190.10837261.014671.20.84.7419.75.60.103021
25.20756225.50.159836143.504894.32.75.207560.214.10.14066
35.44667530.60.059430750.499780.60.45.446674.44.90.0574663
45.674833.90.17154451.500761.81.25.6747.95.20.161391
55.651772350.071841860.79127510.65.651770.96.20.0714688
66.1961036470.12527651.096181.30.86.1961036.74.90.126646
76.39113151.30.13740471.565221.91.26.3911307.10.123878
86.539120454.60.79752967.86769.666.5391203.35.50.803827
96.679124356.40.51833496.899158.45.36.6791245.190.433736
106.783127057.60.72731177.571149.35.86.78312696.60.652046
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.994134060.80.158279123.443944.22.66.994134412.50.155913
127.1321390630.2482983.117583.82.47.1321392.280.221084
137.209141864.30.83318569.5652211.77.37.2091419.56.40.845038
147.261143765.20.18033551.6257821.27.2611436.35.20.17552
157.499152569.20.281616104.796255.93.77.4991524.2100.269549
167.621157071.20.75849579.3696411.57.27.6211568.76.90.770878
178.438188485.40.22973493.796754.62.98.4381882.78.70.247526
188.984206893.80.3533972413.720416.810.58.9842067.323.90.324114
PeakCounter: 18
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 16
LANE TYPE: pI Markers
ACTUAL GEL LANE: 18
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
13.5542.50.326281189.848412.79.83.557.917.70.313253
24.5537617.10.186316175.70857.35.74.55373.717.10.188702
35.255825.30.2898158.0479910.485.2556.515.30.297423
45.8586439.20.67278389.6146312.49.65.85863.88.10.667373
56.55121054.90.53647387.219349.37.26.551210.380.510907
66.85129658.80.44741396.604918.56.66.851295.890.415423
77.351476670.5976471010.369813.410.37.351474.510.10.579566
88.15177180.40.114727173.437164.43.48.151770.617.10.113271
98.45188485.50.208621113.995995.148.451883.411.10.203825
108.65195488.70.185926103.1166343.18.651952.49.60.18224
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
119.3217498.60.523367119.7037212.59.79.32170.911.50.476964
PeakCounter: 11
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 3.50 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 6.55 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 3.50 label for gel schematic
white spacer for gel schematic
pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 94
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 11
LANE TYPE: pI Markers
ACTUAL GEL LANE: 13
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 12
LANE TYPE: Specimen
ACTUAL GEL LANE: 14
PeakCount: 17 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 13
LANE TYPE: pI Markers
ACTUAL GEL LANE: 15
PeakCount: 11 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 14
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 16
PeakCount: 26 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 15
LANE TYPE: Specimen
ACTUAL GEL LANE: 17
PeakCount: 18 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 16
LANE TYPE: pI Markers
ACTUAL GEL LANE: 18
PeakCount: 11 Return to Lane Navigator



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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln