RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Walleye Pollock ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Theragra chalcogramma
FDA Seafood List Common Name: Walleye Pollock
FDA Seafood List Market Name: Pollock (Alaska Pollock)
Unique IEF Gel Code: 19970430gel06
Unique Web Code: wyh04Srr
Tissue Aliquot Code: therchal F96-005 #07m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	28	3.6	0.0329212	5	0.29056	6.5	4.3	4.55	29.2	5.1	0.0320205
2	5.2	120	15.5	0.0345404	6	0.355228	7.9	5.3	5.2	119.9	5.8	0.0344942
3	5.85	232	29.9	0.0688727	5	0.651019	14.5	9.6	5.85	231.7	5.3	0.0698225
4	6.55	370	47.7	0.0260126	9	0.365043	8.1	5.4	6.55	369.1	9	0.0228608
5	6.85	408	52.6	0.0269472	12	0.449765	10	6.7	6.85	410.1	12.1	0.0209218
6	7.35	469	60.4	0.0453082	5	0.3932	8.8	5.8	7.35	468.7	4.9	0.0449512
7	8.15	596	76.8	0.0465628	10	0.749292	16.7	11.1	8.15	595.3	9.6	0.0438901
8	8.45	662	85.3	0.0477298	9	0.727647	16.2	10.8	8.45	660.6	9.1	0.045055
9	8.65	711	91.6	0.0357872	4	0.270214	6	4	8.65	710.2	4.4	0.0348007
10	9.3	756	97.4	0.0294268	5	0.240282	5.3	3.6	9.3	756.7	4.8	0.0283747

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.488	18	2.3	0.034561	3	0.109503	2.1	0.9	4.488	17.4	2.7	0.0228907
2	4.604	34	4.4	0.0521786	12	1.09995	21.4	9.5	4.604	38	12	0.0518748
3	4.779	58	7.5	0.0368593	11	0.636529	12.4	5.5	4.779	57.8	11.5	0.0312703
4	4.982	86	11.1	0.004794	2	0.0093331	0.2	0.1	4.982	85.9	2.1	0.0025241
5	5.156	110	14.2	0.0086657	4	0.0556536	1.1	0.5	5.156	109.6	4.2	0.0075173
6	5.247	124	16	0.0105297	4	0.068847	1.3	0.6	5.247	124.4	3.6	0.0107252
7	5.337	139	17.9	0.0817154	4	0.601018	11.7	5.2	5.337	139.8	4.1	0.0832229
8	5.408	151	19.4	0.0548096	5	0.486095	9.4	4.2	5.408	151	5.3	0.0516218
9	5.857	227	29.2	0.0660543	6	0.691195	13.4	6	5.857	228.4	6	0.0644727
10	5.952	246	31.7	0.0281989	9	0.454814	8.8	3.9	5.952	245.9	9.3	0.0276232
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.101	276	35.5	0.0293229	5	0.243898	4.7	2.1	6.101	277.3	5.3	0.0258154
12	6.185	293	37.7	0.0192191	3	0.0903896	1.8	0.8	6.185	293.8	2.7	0.018907
13	6.289	314	40.4	0.0202172	4	0.134509	2.6	1.2	6.289	313.9	4	0.0190859
14	6.384	333	42.9	0.0160846	3	0.0802552	1.6	0.7	6.384	332.6	3.3	0.0138205
15	6.438	344	44.3	0.0358484	7	0.38889	7.5	3.4	6.438	344.2	6.7	0.0328009

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	26	3.3	0.0366613	5	0.324518	7.6	5	4.55	26.4	5.1	0.0360782
2	5.2	113	14.5	0.0261613	7	0.334501	7.9	5.2	5.2	115.1	6.9	0.0271593
3	5.85	220	28.3	0.0728092	4	0.523994	12.4	8.1	5.85	219.8	4	0.0730689
4	6.55	364	46.8	0.032885	11	0.541244	12.8	8.4	6.55	361.4	10.9	0.0281306
5	6.85	399	51.3	0.0300267	6	0.289707	6.8	4.5	6.85	399.8	6.2	0.0265537
6	7.35	466	59.9	0.0424814	5	0.381793	9	5.9	7.35	464.9	5.1	0.0420081
7	8.15	597	76.7	0.0450858	8	0.590037	13.9	9.2	8.15	596.3	7.5	0.044281
8	8.45	659	84.7	0.0523797	8	0.65879	15.5	10.2	8.45	658.7	7.5	0.0495044
9	8.65	707	90.9	0.0361088	5	0.325237	7.7	5.1	8.65	705.7	5.1	0.0362593
10	9.3	757	97.3	0.0284358	6	0.272449	6.4	4.2	9.3	756.3	6.1	0.0251287

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.467	20	2.6	0.0321695	4	0.220388	3.8	2.4	4.467	20.3	3.8	0.032524
2	4.55	31	4	0.031151	3	0.17273	3	1.9	4.55	30.9	3.2	0.030291
3	4.701	51	6.5	0.0212246	7	0.308767	5.3	3.4	4.701	53.9	7.4	0.0236262
4	4.845	70	9	0.0176877	10	0.339876	5.8	3.7	4.845	65.7	10.1	0.0189087
5	4.935	82	10.5	0.0065329	1	0.001895	0	0	4.935	82.4	0.5	0.0018143
6	5.2	117	15	0.0206898	5	0.181692	3.1	2	5.2	118	5.4	0.0189706
7	5.342	140	17.9	0.0936267	4	0.559823	9.6	6.1	5.342	140	3.5	0.0896467
8	5.417	152	19.5	0.0676448	5	0.492112	8.5	5.3	5.417	151.9	4.5	0.0610962
9	5.85	222	28.4	0.0760551	4	0.473365	8.1	5.1	5.85	222.1	3.8	0.0703505
10	5.944	241	30.9	0.0303613	14	0.618539	10.6	6.7	5.944	241.2	13.7	0.0255366
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.122	277	35.5	0.0301472	2	0.118154	2	1.3	6.122	277.2	2.2	0.0308167
12	6.161	285	36.5	0.0135762	3	0.0786731	1.4	0.9	6.161	284.6	3.2	0.0139014
13	6.221	297	38	0.030526	2	0.128792	2.2	1.4	6.221	297.2	2.4	0.0305164
14	6.324	318	40.7	0.0218773	2	0.0801691	1.4	0.9	6.324	317.7	2.1	0.0219859
15	6.463	346	44.3	0.0433694	7	0.475193	8.2	5.2	6.463	345.8	6.8	0.0392608
16	6.85	396	50.7	0.0245178	5	0.2019	3.5	2.2	6.85	396.1	5.3	0.0215409
17	7.35	466	59.7	0.0245901	4	0.173188	3	1.9	7.35	465.2	4	0.0244771
18	7.446	482	61.7	0.012805	6	0.138034	2.4	1.5	7.446	483.7	6.3	0.0124339
19	8.15	600	76.8	0.0152331	8	0.218344	3.8	2.4	8.15	596.9	8.4	0.0146593
20	8.45	660	84.5	0.0331681	7	0.399654	6.9	4.3	8.45	659.7	7.4	0.0303086
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.65	710	90.9	0.0235253	4	0.14474	2.5	1.6	8.65	709.8	3.5	0.0233276
22	9.3	762	97.6	0.0325396	5	0.287017	4.9	3.1	9.3	762.1	5.4	0.0301564

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.466	22	2.8	0.0509031	5	0.417164	6.4	3.3	4.466	22.9	4.6	0.0507811
2	4.657	47	6	0.0779727	9	1.07979	16.6	8.5	4.657	47.4	8.5	0.071654
3	4.802	66	8.5	0.0517023	12	1.14404	17.6	9	4.802	64.5	12	0.0538628
4	4.948	85	10.9	0.0133333	6	0.108552	1.7	0.9	4.948	83.6	5.7	0.0108348
5	5.139	110	14.1	0.007219	5	0.054974	0.8	0.4	5.139	108.1	5.1	0.0060665
6	5.255	127	16.3	0.010189	5	0.0750314	1.2	0.6	5.255	127.5	4.7	0.0090824
7	5.366	145	18.6	0.088704	5	0.705088	10.9	5.5	5.366	145	4.5	0.0874519
8	5.445	158	20.2	0.0705609	5	0.596917	9.2	4.7	5.445	157.6	4.9	0.0683471
9	5.55	175	22.4	0.021153	10	0.347116	5.3	2.7	5.55	174.4	9.7	0.0202034
10	5.678	196	25.1	0.007263	3	0.0327416	0.5	0.3	5.678	197.1	3.1	0.0059649
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.86	226	28.9	0.0744002	5	0.612432	9.4	4.8	5.86	226.1	5.1	0.0682705
12	5.965	247	31.6	0.024041	11	0.420992	6.5	3.3	5.965	245.8	11.3	0.0209303
13	6.134	281	36	0.0358636	5	0.262745	4	2.1	6.134	281.1	4.7	0.0312253
14	6.174	289	37	0.0185932	3	0.0840876	1.3	0.7	6.174	288.7	2.7	0.0178065
15	6.224	299	38.3	0.0318855	2	0.128289	2	1	6.224	298.9	2.2	0.0333222
16	6.319	318	40.7	0.0254763	2	0.0946943	1.5	0.7	6.319	318.1	2.1	0.0251113
17	6.459	346	44.3	0.038148	5	0.333351	5.1	2.6	6.459	346.3	5.1	0.0369933

PeakCounter: 17

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	33	4.2	0.0527423	4	0.383808	7.5	5.1	4.55	33.3	4.2	0.0510472
2	5.2	117	15	0.0444419	5	0.36964	7.2	4.9	5.2	117.4	5	0.0417121
3	5.85	224	28.7	0.078625	4	0.598364	11.6	8	5.85	223.4	4.3	0.0788883
4	6.55	363	46.5	0.0357775	16	0.912085	17.8	12.2	6.55	359.8	16.3	0.031611
5	6.85	400	51.2	0.0293176	4	0.203267	4	2.7	6.85	399.9	4.3	0.0269267
6	7.35	470	60.2	0.0398769	5	0.346377	6.7	4.6	7.35	469.4	5	0.0393952
7	8.15	594	76.1	0.0609378	7	0.761806	14.8	10.2	8.15	593.4	7.4	0.0580788
8	8.45	655	83.9	0.0523748	9	0.792624	15.4	10.6	8.45	654.7	8.9	0.0502464
9	8.65	712	91.2	0.0448414	4	0.319859	6.2	4.3	8.65	711.1	4.2	0.0434758
10	9.3	768	98.3	0.0519706	5	0.45062	8.8	6	9.3	767.8	5.2	0.0488978

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 84

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 6.500 5.200 7.900 5.850 14.500 6.550 8.100 6.850 10.000 7.350 8.800 8.150 16.700 8.450 16.200 8.650 6.000 9.300 5.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.488 2.100 4.604 21.400 4.779 12.400 4.982 0.200 5.156 1.100 5.247 1.300 5.337 11.700 5.408 9.400 5.857 13.400 5.952 8.800 6.101 4.700 6.185 1.800 6.289 2.600 6.384 1.600 6.438 7.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 15 Return to Lane Navigator
4.550 7.600 5.200 7.900 5.850 12.400 6.550 12.800 6.850 6.800 7.350 9.000 8.150 13.900 8.450 15.500 8.650 7.700 9.300 6.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.467 3.800 4.550 3.000 4.701 5.300 4.845 5.800 4.935 0 5.200 3.100 5.342 9.600 5.417 8.500 5.850 8.100 5.944 10.600 6.122 2.000 6.161 1.400 6.221 2.200 6.324 1.400 6.463 8.200 6.850 3.500 7.350 3.000 7.446 2.400 8.150 3.800 8.450 6.900 8.650 2.500 9.300 4.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 22 Return to Lane Navigator
4.466 6.400 4.657 16.600 4.802 17.600 4.948 1.700 5.139 0.800 5.255 1.200 5.366 10.900 5.445 9.200 5.550 5.300 5.678 0.500 5.860 9.400 5.965 6.500 6.134 4.000 6.174 1.300 6.224 2.000 6.319 1.500 6.459 5.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 17 Return to Lane Navigator
4.550 7.500 5.200 7.200 5.850 11.600 6.550 17.800 6.850 4.000 7.350 6.700 8.150 14.800 8.450 15.400 8.650 6.200 9.300 8.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Walleye Pollock ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Walleye Pollock ---- Go to Display Gel Lane Navigator.