Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Walleye Pollock  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Theragra chalcogramma
FDA Seafood List Common Name: Walleye Pollock
FDA Seafood List Market Name: Pollock (Alaska Pollock)
Unique IEF Gel Code: 19930921gel03
Unique Web Code: wyh02Srr
Tissue Aliquot Code: therchal 002 #1 Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Co-Migrated
Lane c
Specimen
Lane d
pI Markers
Lane e
Co-Migrated
Lane f
Specimen
Lane g
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 1
LANE TYPE: pI Markers
ACTUAL GEL LANE: 1
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55384.70.038640940.2591555.74.24.5537.93.80.0381396
25.213316.60.066477540.41021196.65.21333.60.0634961
35.8523829.70.10995730.61718413.59.95.852383.30.106328
46.5537146.30.061448560.6407221410.36.55370.26.10.0589747
56.8540550.60.012197970.1111192.41.86.85405.57.30.0085841
67.3547359.10.039922240.2571395.64.17.35472.73.70.0396055
78.1559674.40.049114870.64184214.110.38.15595.17.50.0483569
88.4566382.80.036567780.51818711.48.38.45663.18.20.0358393
98.6571589.30.036392450.2997326.64.88.65715.650.0337046
109.377396.50.09852850.80701817.712.99.3773.14.90.092981
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 2
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55384.70.335956127.4684525.919.54.5538.812.30.342597
24.734658.10.184828134.0125313.910.54.73461.713.40.169345
34.9259311.60.0228408201.170214.13.14.92579.720.10.032847
45.12312215.20.010242840.04452420.20.15.1231223.60.0070389
55.25414217.70.029459540.2212740.80.65.2541424.10.0304049
65.35315819.70.58574143.4324411.995.353158.13.60.540036
75.43417121.30.13246292.351148.26.15.434167.18.60.154892
85.515184230.050726690.7362192.61.95.515184.28.90.0467345
95.713216270.0247769100.4562571.61.25.71321910.50.0246117
105.89224630.70.41021353.7837613.19.95.892245.35.10.418138
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.98126332.80.23197772.531498.86.65.981262.26.60.215165
126.13929336.60.090312530.518611.81.46.139292.93.50.083839
136.16529837.20.084033130.3970981.416.165298.72.80.0787312
146.22831038.70.066850720.2620180.90.76.228309.82.30.0649248
156.32332840.90.045254220.1752350.60.56.3233282.20.0459615
166.46435544.30.15345251.255274.43.36.464355.250.141582
PeakCounter: 16
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.652526.50.288693104.427917.113.54.65250.310.10.247932
24.801749.20.212806186.733525.920.54.80172180.211058
34.98510112.60.045202640.09320590.40.34.98599.440.0130555
45.0811514.40.0172603110.4282551.71.35.08107.710.90.0221067
55.17512916.1030.0103756005.17512930.0019513
65.31415118.90.027180450.2316930.90.75.314150.94.90.0266274
75.40116520.60.68571533.11301129.55.401164.82.70.644335
85.45117321.60.14629761.401145.44.35.451172.26.10.129423
95.58819524.30.026419161.140224.43.55.588180.116.50.0390543
105.74422027.50.0315046100.4411971.71.35.744220.99.70.0256455
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.89224530.60.57961333.3374512.910.25.892245.63.50.545481
125.98126232.70.26924252.003347.76.15.981262.14.70.241179
136.13329136.30.083230710.1867530.70.66.133290.11.30.0788499
146.16429737.10.079905540.5791412.21.86.164296.44.10.0787782
156.22230838.50.087625530.4023891.61.26.222307.82.80.0805981
166.31132540.60.053005630.25468510.86.3113252.80.0514639
176.45735344.10.14881151.164914.53.66.457353.24.80.137084
PeakCounter: 17
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55374.60.053126140.3570426.754.5537.53.90.0513269
25.213316.60.067368140.42205385.95.2132.63.60.0656004
35.8523729.60.11867940.75462514.210.55.85236.63.70.114096
46.5537146.30.069689670.83784415.811.76.55370.27.20.0656019
56.8540450.40.012534950.1010131.91.46.85403.74.80.011922
67.3547158.80.044081840.3361256.34.77.35471.34.40.0431435
78.1559674.40.058517770.70089413.29.88.15596.57.10.0556637
88.4566282.60.032662590.5044699.57.18.45661.38.50.0333896
98.6571288.90.032316160.3391726.44.78.65712.56.10.0316224
109.377196.30.10435650.94794817.913.29.3771.25.40.0983505
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.623496.10.242597177.0994734.326.74.6235016.80.238158
24.9239411.70.0248779161.315076.44.94.9237815.80.0470075
34.989104130.014770340.06496840.30.24.989104.23.80.0096712
45.17613216.40.018333780.2207771.10.85.176132.37.70.0162726
55.23414117.60.020371630.08504570.40.35.234141.330.0160026
65.33515719.60.5336633.0335614.711.45.335157.23.30.515447
75.42317121.30.078319391.399376.85.35.423166.78.70.0910934
85.562193240.0213129100.4411692.11.75.562185.8100.0249634
95.713217270.016872270.20755110.85.7132187.40.0158866
105.87324330.30.39031242.5134812.29.45.873242.83.80.377616
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.96226032.40.18404951.551827.55.85.962259.75.10.17032
126.10928835.90.065456440.3679741.81.46.109288.53.60.0577069
136.15129636.90.037665420.2112910.86.151294.82.40.0489878
146.20430638.10.059244830.2869281.41.16.204305.92.80.057922
156.29332340.20.035584730.19782410.76.293323.73.30.0337197
166.4435143.70.11050450.9050534.43.46.44351.14.70.10759
177.3547058.50.017388240.1170430.60.47.35470.140.0166717
188.4566382.60.026646680.2639311.318.45662.68.10.0184053
199.377396.30.027865690.396111.91.59.37729.10.0244482
PeakCounter: 19
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.609455.60.334394104.9355918.614.34.60945.1100.279618
24.792739.10.177289196.6684725.219.34.79265.118.80.199719
34.94396120.022300940.05751960.20.24.94397.23.60.0090875
45.13212515.60.0177526100.26466610.85.132130.810.50.0142784
55.26814618.20.03195650.26606610.85.268145.44.60.0324175
65.3716220.20.62308633.697291410.75.37162.13.50.602992
75.44617421.70.1479871.5934264.65.446172.16.80.131805
85.57419424.20.0366279141.011193.82.95.574187.5140.0407906
95.7422027.50.029130480.3669571.41.15.74221.38.40.0247363
105.89624630.70.52017843.2045112.19.35.896246.73.60.506751
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.98526332.80.23989451.970627.45.75.985262.850.223822
126.13329136.30.097813320.3676551.41.16.133290.82.20.0939843
136.16529737.10.085035630.4923821.91.46.165296.43.10.0889837
146.22330838.50.073840920.2840251.10.86.223307.92.20.0719984
156.31832640.70.045316520.1651650.60.56.318326.22.10.044968
166.46635444.20.145751.128434.33.36.466353.64.70.136651
PeakCounter: 16
DISPLAY GEL LANE LETTER: Lane g
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55354.40.048997140.356216.74.94.5535.54.30.0471785
25.2136170.08476540.517799.87.15.2136.23.50.0828635
35.8523729.60.12118640.80626515.211.15.85237.33.80.118285
46.5536946.10.060884280.81615715.411.26.55367.37.80.0590558
56.8539949.80.010638540.07374421.416.8540040.0104355
67.3546858.40.03989940.26616653.77.35467.640.0378378
78.1559273.90.052423670.62822711.88.68.15592.26.80.051913
88.4566282.60.043130870.5085749.678.45662.16.70.0428651
98.6571188.80.055048940.3785397.15.28.65710.540.0533214
109.376895.90.11136150.9566481813.29.3768.35.10.10599
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f g
MK CO SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 6.85 label for gel schematic
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pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 8.65 label for gel schematic
white spacer for gel schematic
pI 9.30 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 98
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 1
LANE TYPE: pI Markers
ACTUAL GEL LANE: 1
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 2
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 2
PeakCount: 16 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 17 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 19 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 16 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane g
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln