RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Walleye Pollock ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Theragra chalcogramma
FDA Seafood List Common Name: Walleye Pollock
FDA Seafood List Market Name: Pollock (Alaska Pollock)
Unique IEF Gel Code: 19930921gel01
Unique Web Code: wyg02Srr
Tissue Aliquot Code: therchal 002 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 10
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 12
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	61	5.3	0.302579	11	5.44037	24.6	18.1	3.5	62.5	11	0.27802
2	4.55	202	17.4	0.104338	12	2.05925	9.3	6.9	4.55	202.2	11.9	0.097775
3	5.2	278	24	0.0897672	11	1.62575	7.3	5.4	5.2	277.2	10.8	0.085286
4	5.85	424	36.6	0.275778	5	2.19189	9.9	7.3	5.85	423.6	4.7	0.263394
5	6.55	579	49.9	0.12865	7	1.38628	6.3	4.6	6.55	578.4	7.2	0.109123
6	7.35	711	61.3	0.0108232	8	0.107863	0.5	0.4	7.35	713	7.5	0.00806331
7	8.15	882	76	0.0259703	10	0.410293	1.9	1.4	8.15	883.1	9.8	0.0235887
8	8.45	947	81.6	0.0739491	8	0.996456	4.5	3.3	8.45	946.9	8.3	0.0675572
9	8.65	987	85.1	0.0362335	8	0.479575	2.2	1.6	8.65	986.1	8	0.0336816
10	9.3	1113	95.9	0.479689	9	7.45115	33.6	24.8	9.3	1110.4	9.3	0.450022

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	60	5.2	0.0982902	10	1.62777	3.7	3.3	3.5	60.4	9.9	0.0925146
2	3.729	89	7.7	0.0754376	7	1.53084	3.5	3.1	3.729	94.2	6.6	0.131771
3	3.847	104	9	0.571654	7	6.67364	15.2	13.3	3.847	104.2	6.6	0.571904
4	4.021	126	10.9	0.0671142	7	0.843565	1.9	1.7	4.021	124.6	7.3	0.0656449
5	4.147	142	12.2	0.0287828	8	0.438337	1	0.9	4.147	138	8.1	0.030669
6	4.274	158	13.6	0.186995	5	1.53681	3.5	3.1	4.274	158.3	4.7	0.185473
7	4.432	178	15.3	0.0267057	4	0.109804	0.2	0.2	4.432	177.7	3.8	0.0161116
8	4.55	193	16.6	0.0833694	11	1.50235	3.4	3	4.55	193.2	10.7	0.0789406
9	4.795	219	18.9	0	17	0.0345523	0.1	0.1	4.795	226.2	16.5	0.00117851
10	4.87	227	19.6	0.0362161	1	0.0739511	0.2	0.1	4.87	227	1.1	0.0383841
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.021	243	20.9	0.0915929	8	1.14824	2.6	2.3	5.021	243	7.7	0.0844447
12	5.2	262	22.6	0.112569	2	0.367325	0.8	0.7	5.2	261.9	1.9	0.106302
13	5.248	273	23.5	0.362953	9	5.83306	13.3	11.7	5.248	273.2	9.2	0.358773
14	5.321	290	25	0.10009	5	0.698108	1.6	1.4	5.321	289.6	4.7	0.0838763
15	5.603	355	30.6	0.038809	8	0.541323	1.2	1.1	5.603	354.1	8.4	0.0364265
16	5.72	382	32.9	0.407567	11	8.24049	18.7	16.5	5.72	381.2	11	0.42103
17	5.85	412	35.5	0.272	8	3.74175	8.5	7.5	5.85	411.7	8.5	0.249389
18	6	444	38.3	0.0176396	24	1.07633	2.4	2.2	6	428.2	24	0.0253028
19	6.085	462	39.8	0.00866161	1	0	0	0	6.085	458	1	0
20	6.188	484	41.7	0.0202338	2	0.0792686	0.2	0.2	6.188	484	2.2	0.0205642
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.367	522	45	0.00702121	2	0.0247169	0.1	0	6.367	522	2.2	0.00628557
22	6.55	561	48.4	0.0145175	13	0.284972	0.6	0.6	6.55	560.2	13.4	0.012002
23	7.35	710	61.2	0.017684	5	0.134763	0.3	0.3	7.35	709.8	5.4	0.0141411
24	8.15	882	76	0.180848	6	1.62041	3.7	3.2	8.15	881.9	6	0.152003
25	8.45	941	81.1	0.0192095	8	0.229053	0.5	0.5	8.45	941.1	7.6	0.01707
26	8.65	985	84.9	0.0174132	8	0.248371	0.6	0.5	8.65	985.1	8.4	0.0166439
27	9.3	1112	95.9	0.383782	9	5.35159	12.2	10.7	9.3	1109.8	8.8	0.34294

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.796	102	8.8	0.112946	5	0.636194	2.3	2	3.796	102.3	4.6	0.0778955
2	3.892	115	9.9	0.619529	8	8.51773	31	26.1	3.892	114.9	7.8	0.617183
3	4.077	140	12	0.109228	12	2.18445	8	6.7	4.077	142.9	12	0.102706
4	4.313	172	14.8	0.198622	4	1.49184	5.4	4.6	4.313	171.6	4.4	0.193362
5	4.483	195	16.8	0.013768	4	0.0851301	0.3	0.3	4.483	193.5	3.8	0.0125681
6	4.632	213	18.3	0.0325418	8	0.440921	1.6	1.4	4.632	212.9	8.3	0.0299542
7	4.859	238	20.5	0.00859425	2	0.0244995	0.1	0.1	4.859	237.7	1.7	0.00818976
8	4.959	249	21.4	0.0115807	4	0.0841211	0.3	0.3	4.959	248.8	4.4	0.0106849
9	5.114	266	22.9	0.0187138	6	0.163908	0.6	0.5	5.114	265.7	6.2	0.0148942
10	5.233	283	24.3	0.0539253	5	0.444818	1.6	1.4	5.233	283	4.7	0.0531619
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.282	294	25.3	0.646733	4	4.4748	16.3	13.7	5.282	293.6	3.9	0.644776
12	5.33	305	26.2	0.0697685	4	0.633818	2.3	1.9	5.33	303.4	3.9	0.0916645
13	5.59	364	31.3	0.0160337	3	0.0728525	0.3	0.2	5.59	363.6	2.5	0.0163854
14	5.7	389	33.4	0.624485	5	5.29049	19.3	16.2	5.7	390	4.9	0.606419
15	5.828	418	35.9	0.22787	6	2.07327	7.6	6.4	5.828	417.8	5.9	0.199013
16	5.922	439	37.7	0.0282213	11	0.604251	2.2	1.9	5.922	434.2	11.1	0.0306463
17	5.971	450	38.7	0.0119368	4	0.0587443	0.2	0.2	5.971	449.5	4.4	0.00752753
18	6.029	463	39.8	0.0140882	3	0.059115	0.2	0.2	6.029	463.6	2.7	0.0122738
19	6.127	485	41.7	0.0130679	4	0.0790502	0.3	0.2	6.127	484.8	3.8	0.0118923
20	6.32	528	45.4	0.00792683	1	0.0127355	0	0	6.32	527.9	0.9	0.00799754

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	56	4.8	0.217783	9	3.30368	20.3	14.2	3.5	59.4	9.1	0.205345
2	4.55	207	17.9	0.0904183	11	1.66627	10.2	7.2	4.55	206.2	11.5	0.0818083
3	5.2	281	24.3	0.0698513	10	1.06153	6.5	4.6	5.2	281.3	9.6	0.062422
4	5.85	426	36.8	0.273369	4	1.73555	10.7	7.5	5.85	426	3.8	0.260429
5	6.55	590	50.9	0.128876	6	1.16376	7.2	5	6.55	589.5	6.1	0.108068
6	7.35	722	62.3	0.01219	7	0.109475	0.7	0.5	7.35	721	6.8	0.0090613
7	8.15	883	76.3	0	4	0.0321281	0.2	0.1	8.15	883	4	0.00453163
8	8.45	950	82	0.0409844	6	0.437061	2.7	1.9	8.45	950.4	6.5	0.0381108
9	8.65	989	85.4	0.0255451	5	0.233583	1.4	1	8.65	989.4	5.5	0.0240609
10	9.3	1115	96.3	0.456203	9	6.52668	40.1	28.1	9.3	1112.4	8.7	0.423007

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	59	5.1	0.232288	9	3.63913	6	5.4	3.5	60.8	9.4	0.218712
2	3.777	97	8.4	0.109151	6	0.93016	1.5	1.4	3.777	98.6	5.8	0.0901216
3	3.872	110	9.5	0.535147	8	7.0164	11.5	10.5	3.872	110.2	7.6	0.522573
4	4.032	132	11.4	0.240601	11	4.81338	7.9	7.2	4.032	130.3	11.5	0.236837
5	4.164	150	13	0.140356	11	2.3691	3.9	3.5	4.164	148.7	10.7	0.12533
6	4.288	167	14.4	0.168992	6	1.70323	2.8	2.6	4.288	167.1	5.8	0.165798
7	4.448	189	16.3	0.0291634	4	0.105251	0.2	0.2	4.448	189.1	4.5	0.0133244
8	4.55	203	17.5	0.0878262	12	1.84321	3	2.8	4.55	204.1	11.9	0.0871721
9	4.964	252	21.8	0.0667269	23	3.46971	5.7	5.2	4.964	265.9	23.2	0.0845293
10	5.2	280	24.2	0.133224	15	3.48353	5.7	5.2	5.2	290.2	15.4	0.127704
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.284	298	25.7	0.435024	6	3.08894	5.1	4.6	5.284	296.7	6	0.291386
12	5.414	326	28.2	0.00804973	1	0	0	0	5.414	316	1	0
13	5.506	346	29.9	0.00782784	7	0.0858674	0.1	0.1	5.506	347.9	6.6	0.00735178
14	5.609	368	31.8	0.0481147	15	1.74781	2.9	2.6	5.609	371.2	15.2	0.0649515
15	5.641	375	32.4	0.0626182	7	0.500292	0.8	0.7	5.641	387.3	6.7	0.0418292
16	5.739	396	34.2	0.643585	6	6.05778	9.9	9.1	5.739	396.1	5.8	0.59355
17	5.85	420	36.3	0.385156	7	4.15942	6.8	6.2	5.85	419.9	6.8	0.342794
18	5.961	443	38.3	0.0333222	17	1.00877	1.7	1.5	5.961	438.5	17	0.0334168
19	6.091	470	40.6	0.0141887	1	0.0128158	0	0	6.091	469.7	0.8	0.00909975
20	6.198	492	42.5	0.0280602	2	0.096973	0.2	0.1	6.198	492	2	0.0274564
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.371	528	45.6	0.0183324	2	0.065806	0.1	0.1	6.371	527.5	2.2	0.0167505
22	6.55	565	48.8	0.0369446	16	1.03734	1.7	1.6	6.55	566.9	16.4	0.0356802
23	7.35	717	61.9	0.0717483	6	0.748522	1.2	1.1	7.35	716.2	6.5	0.0649833
24	8.15	879	75.9	0.478158	5	3.77306	6.2	5.7	8.15	878.6	4.9	0.436275
25	8.45	945	81.6	0.0394649	10	0.724339	1.2	1.1	8.45	945.5	10.4	0.0391414
26	8.65	987	85.2	0.0497883	7	0.596269	1	0.9	8.65	987.6	7.3	0.0457729
27	9.3	1113	96.1	0.453346	11	7.86684	12.9	11.8	9.3	1109.8	10.7	0.415741

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.794	98	8.5	0.0907277	3	0.325562	1.3	1.1	3.794	97.5	3.4	0.0534585
2	3.876	109	9.4	0.538931	6	6.16644	24.4	21.5	3.876	108.4	6.4	0.544707
3	4.012	127	11	0.124937	10	2.41665	9.6	8.4	4.012	125.4	10.3	0.131753
4	4.177	149	12.9	0.0233866	3	0.0796315	0.3	0.3	4.177	150.1	2.5	0.017668
5	4.305	166	14.3	0.2157	4	1.47349	5.8	5.1	4.305	166.1	3.8	0.217822
6	4.478	189	16.3	0.0151713	4	0.0933951	0.4	0.3	4.478	188.4	4	0.0132249
7	4.633	208	18	0.0352078	9	0.521736	2.1	1.8	4.633	207.7	8.9	0.033224
8	4.851	233	20.1	0	1	0	0	0	4.851	232	1	0
9	4.973	247	21.3	0.0105719	4	0.0795461	0.3	0.3	4.973	246	4.3	0.0104106
10	5.043	255	22	0.0116794	4	0.0815741	0.3	0.3	5.043	256.1	4.4	0.010446
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.156	268	23.2	0.0177625	11	0.172822	0.7	0.6	5.156	270.3	11.4	0.00859036
12	5.291	293	25.3	0.476226	7	5.74628	22.8	20	5.291	291.1	6.9	0.467829
13	5.405	318	27.5	0.00919946	4	0.0564462	0.2	0.2	5.405	319.2	4.2	0.00753486
14	5.586	358	30.9	0.00436601	3	0.0281346	0.1	0.1	5.586	356.8	2.8	0.00561429
15	5.614	364	31.5	0.0105011	3	0.0446369	0.2	0.2	5.614	364.7	2.6	0.00985597
16	5.673	377	32.6	0.0143196	5	0.0876371	0.3	0.3	5.673	377.3	4.7	0.0104899
17	5.745	393	34	0.593166	5	4.90395	19.4	17.1	5.745	393.4	4.7	0.584655
18	5.868	420	36.3	0.293331	5	2.55118	10.1	8.9	5.868	420	5.1	0.27988
19	5.963	441	38.1	0.00636369	2	0.0287832	0.1	0.1	5.963	441.8	2.5	0.00660202
20	6.085	468	40.4	0.00429025	2	0.195792	0.8	0.7	6.085	464.6	2.3	0.0484654
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.166	486	42	0.0351307	2	0.133249	0.5	0.5	6.166	486.3	2.2	0.0347801
22	6.32	520	44.9	0.0119853	3	0.0558174	0.2	0.2	6.32	520.5	2.9	0.0109962

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	61	5.3	0.286563	13	6.23387	29.1	21.5	3.5	63.6	13.2	0.265965
2	4.55	196	16.9	0.0938802	11	1.60307	7.5	5.5	4.55	196	10.6	0.0852987
3	5.2	268	23.1	0.0667811	7	0.799497	3.7	2.8	5.2	267.9	7.2	0.0625827
4	5.85	414	35.8	0.317564	4	2.13428	10	7.4	5.85	414.3	3.9	0.307896
5	6.55	579	50	0.168825	7	1.71125	8	5.9	6.55	578.5	6.6	0.146937
6	7.35	716	61.8	0.0299447	6	0.309834	1.4	1.1	7.35	714.2	6.3	0.0278529
7	8.15	881	76.1	0.0269457	10	0.39322	1.8	1.4	8.15	882.1	10	0.0221606
8	8.45	941	81.3	0.0416276	7	0.490562	2.3	1.7	8.45	941.7	7	0.0397893
9	8.65	984	85	0.0331694	7	0.370241	1.7	1.3	8.65	983	6.8	0.0307479
10	9.3	1110	95.9	0.446292	10	7.37277	34.4	25.4	9.3	1107.4	10.4	0.401015

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
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PeakCount: 126

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 24.600 4.550 9.300 5.200 7.300 5.850 9.900 6.550 6.300 7.350 0.500 8.150 1.900 8.450 4.500 8.650 2.200 9.300 33.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 10 LANE TYPE: pI Markers ACTUAL GEL LANE: 12 PeakCount: 10 Return to Lane Navigator
3.500 3.700 3.729 3.500 3.847 15.200 4.021 1.900 4.147 1.000 4.274 3.500 4.432 0.200 4.550 3.400 4.795 0.100 4.870 0.200 5.021 2.600 5.200 0.800 5.248 13.300 5.321 1.600 5.603 1.200 5.720 18.700 5.850 8.500 6.000 2.400 6.085 0 6.188 0.200 6.367 0.100 6.550 0.600 7.350 0.300 8.150 3.700 8.450 0.500 8.650 0.600 9.300 12.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 11 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 13 PeakCount: 27 Return to Lane Navigator
3.796 2.300 3.892 31.000 4.077 8.000 4.313 5.400 4.483 0.300 4.632 1.600 4.859 0.100 4.959 0.300 5.114 0.600 5.233 1.600 5.282 16.300 5.330 2.300 5.590 0.300 5.700 19.300 5.828 7.600 5.922 2.200 5.971 0.200 6.029 0.200 6.127 0.300 6.320 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 20 Return to Lane Navigator
3.500 20.300 4.550 10.200 5.200 6.500 5.850 10.700 6.550 7.200 7.350 0.700 8.150 0.200 8.450 2.700 8.650 1.400 9.300 40.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 10 Return to Lane Navigator
3.500 6.000 3.777 1.500 3.872 11.500 4.032 7.900 4.164 3.900 4.288 2.800 4.448 0.200 4.550 3.000 4.964 5.700 5.200 5.700 5.284 5.100 5.414 0 5.506 0.100 5.609 2.900 5.641 0.800 5.739 9.900 5.850 6.800 5.961 1.700 6.091 0 6.198 0.200 6.371 0.100 6.550 1.700 7.350 1.200 8.150 6.200 8.450 1.200 8.650 1.000 9.300 12.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 27 Return to Lane Navigator
3.794 1.300 3.876 24.400 4.012 9.600 4.177 0.300 4.305 5.800 4.478 0.400 4.633 2.100 4.851 0 4.973 0.300 5.043 0.300 5.156 0.700 5.291 22.800 5.405 0.200 5.586 0.100 5.614 0.200 5.673 0.300 5.745 19.400 5.868 10.100 5.963 0.100 6.085 0.800 6.166 0.500 6.320 0.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 22 Return to Lane Navigator
3.500 29.100 4.550 7.500 5.200 3.700 5.850 10.000 6.550 8.000 7.350 1.400 8.150 1.800 8.450 2.300 8.650 1.700 9.300 34.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Walleye Pollock ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Walleye Pollock ---- Go to Display Gel Lane Navigator.