RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Silk Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lutjanus vivanus
FDA Seafood List Common Name: Silk Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19940901gel01
Unique Web Code: ssg01Srr
Tissue Aliquot Code: lutjviva H94-004 #05a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Specimen	Lane e Co-Migrated	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 18
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 20
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	35	3.1	0.14484	12	2.78145	14.4	10.7	3.5	35.4	11.6	0.134849
2	4.55	183	16.3	0.101979	9	1.5525	8	6	4.55	181.3	9	0.096822
3	5.2	262	23.4	0.138437	8	1.8284	9.4	7	5.2	259.8	7.6	0.136631
4	5.85	404	36.1	0.250671	4	1.76687	9.1	6.8	5.85	404.2	3.9	0.254916
5	6.55	569	50.8	0.218536	4	1.48598	7.7	5.7	6.55	568.1	4.1	0.205657
6	6.85	607	54.2	0.0686263	6	0.655582	3.4	2.5	6.85	607.5	6.2	0.059797
7	7.35	698	62.3	0.174155	4	1.32057	6.8	5.1	7.35	698.1	4.4	0.171127
8	8.15	855	76.3	0.0771845	8	1.10087	5.7	4.2	8.15	855.9	8.4	0.0740059
9	8.45	928	82.9	0.0969401	6	0.970279	5	3.7	8.45	927.6	5.8	0.0940598
10	8.65	973	86.9	0.0960003	5	0.806579	4.2	3.1	8.65	972.7	4.9	0.0925446
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1096	97.9	0.339033	9	5.09222	26.3	19.6	9.3	1093.4	9.1	0.315024

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 17
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 19
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.819	82	7.3	0.0603847	7	0.701669	3.5	2.8	3.819	80.7	6.7	0.0590407
2	4.295	149	13.3	0.286582	4	2.16295	10.6	8.6	4.295	148.6	4.2	0.291022
3	4.458	172	15.4	0.0185591	5	0.14674	0.7	0.6	4.458	171.9	4.8	0.0172176
4	4.599	191	17.1	0.0153266	8	0.218744	1.1	0.9	4.599	195.7	7.6	0.0161552
5	4.723	206	18.4	0.0153503	3	0.120249	0.6	0.5	4.723	204	3	0.0228669
6	4.863	223	19.9	0.018966	6	0.172592	0.8	0.7	4.863	224.3	6.5	0.015045
7	4.994	239	21.3	0.0501927	3	0.22377	1.1	0.9	4.994	239.1	2.6	0.0491029
8	5.109	253	22.6	0.0553024	3	0.276338	1.4	1.1	5.109	253.2	3	0.052626
9	5.209	266	23.8	0.0182667	9	0.225737	1.1	0.9	5.209	265.6	8.9	0.0143781
10	5.255	276	24.6	0.0250306	4	0.142479	0.7	0.6	5.255	276.2	3.6	0.0224804
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.327	292	26.1	0.28309	2	0.910469	4.5	3.6	5.327	291.9	1.9	0.275723
12	5.636	360	32.1	0.304038	3	1.76531	8.7	7	5.636	360.2	3.3	0.306027
13	5.782	392	35	0.0845157	3	0.4578	2.3	1.8	5.782	391.4	3.3	0.0791758
14	5.854	408	36.4	0.0536501	6	0.396802	2	1.6	5.854	409.1	5.8	0.0385162
15	5.979	438	39.1	0.431065	3	2.21392	10.9	8.8	5.979	438.5	2.8	0.440373
16	6.058	457	40.8	0.363642	3	1.70845	8.4	6.8	6.058	457.1	2.8	0.342583
17	6.124	473	42.2	0.0749553	7	0.866277	4.3	3.4	6.124	472.8	7	0.0696492
18	6.153	480	42.9	0.152051	2	0.528302	2.6	2.1	6.153	480.1	2.4	0.126454
19	6.411	542	48.4	0.0251373	2	0.0972274	0.5	0.4	6.411	542.6	2.4	0.0225082
20	6.448	551	49.2	0.0305016	6	0.27873	1.4	1.1	6.448	551.2	5.9	0.0268016
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.506	565	50.4	0.0914735	3	0.51646	2.5	2	6.506	564.7	3.3	0.0888232
22	6.64	587	52.4	0.203931	8	2.76037	13.6	10.9	6.64	587.3	8	0.195704
23	6.71	596	53.2	0.114209	2	0.232648	1.1	0.9	6.71	595	1.6	0.081504
24	6.877	619	55.3	0.0738724	4	0.565609	2.8	2.2	6.877	618.3	4.5	0.0713805
25	7.668	768	68.6	0.016617	9	0.310957	1.5	1.2	7.668	771.8	8.9	0.0198178
26	7.786	791	70.6	0.092546	4	0.718791	3.5	2.9	7.786	792.1	4.4	0.0916949
27	8.808	1004	89.6	0.0722161	11	1.38149	6.8	5.5	8.808	1002.9	11.3	0.069017
28	8.994	1040	92.9	0.0166004	9	0.216683	1.1	0.9	8.994	1038.5	8.7	0.0140543
29	9.067	1054	94.1	0.00807054	2	0.0138704	0.1	0.1	9.067	1054.6	1.9	0.00420226

PeakCounter: 29

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	36	3.2	0.154584	12	2.9372	17.6	12.5	3.5	36.2	11.6	0.142334
2	4.55	184	16.5	0.153619	5	1.19711	7.2	5.1	4.55	184	5	0.136223
3	5.2	263	23.5	0.230171	5	1.7677	10.6	7.5	5.2	262.5	4.6	0.217704
4	5.85	407	36.4	0.31529	3	1.62102	9.7	6.9	5.85	407.1	2.9	0.313979
5	6.55	579	51.8	0.198348	4	1.35688	8.1	5.8	6.55	578.5	4.2	0.183911
6	6.85	618	55.3	0.0682983	6	0.655023	3.9	2.8	6.85	617.9	6.3	0.0591011
7	7.35	710	63.6	0.17017	4	1.2896	7.7	5.5	7.35	709.6	4.4	0.165075
8	8.15	867	77.6	0.0634224	9	0.97775	5.8	4.2	8.15	867.1	9.1	0.0606659
9	8.45	931	83.3	0.0854758	7	1.00076	6	4.3	8.45	930.4	6.6	0.0851455
10	8.65	971	86.9	0.0815577	5	0.720896	4.3	3.1	8.65	971.3	5.4	0.0752099
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1099	98.4	0.321433	6	3.20766	19.2	13.6	9.3	1097.5	6.2	0.293825

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.807	85	7.6	0.0570555	7	0.716941	3.9	3.1	3.807	84.6	7.2	0.0563537
2	4.314	157	14	0.284823	4	2.10337	11.4	9.2	4.314	156.6	4.1	0.286904
3	4.476	180	16.1	0.0226307	4	0.162457	0.9	0.7	4.476	180.6	4.5	0.0204814
4	4.637	201	17.9	0.0200375	7	0.219803	1.2	1	4.637	202.8	6.6	0.018761
5	4.736	213	19	0.0307522	4	0.179232	1	0.8	4.736	212.7	3.7	0.0274748
6	4.894	232	20.7	0.0174526	8	0.199772	1.1	0.9	4.894	232.8	8	0.0140393
7	5.034	249	22.2	0.0500507	2	0.214799	1.2	0.9	5.034	249.1	2.5	0.04913
8	5.159	264	23.6	0.0458991	3	0.213925	1.2	0.9	5.159	264.4	2.5	0.0481793
9	5.245	279	24.9	0.0084618	4	0.0491663	0.3	0.2	5.245	278.2	3.5	0.00790324
10	5.281	287	25.6	0.0148515	18	0.326267	1.8	1.4	5.281	298.6	18	0.0102267
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.362	305	27.2	0.271964	2	0.831226	4.5	3.6	5.362	304.7	1.8	0.259944
12	5.668	373	33.3	0.271677	3	1.70535	9.3	7.5	5.668	373.4	3.5	0.277102
13	5.812	405	36.1	0.0687952	4	0.44647	2.4	2	5.812	404.7	4	0.0628407
14	5.881	421	37.6	0.0517729	6	0.38121	2.1	1.7	5.881	422.6	5.6	0.0385302
15	6.004	451	40.2	0.433834	3	2.2353	12.2	9.8	6.004	450.7	2.9	0.437373
16	6.077	469	41.8	0.320887	3	1.6488	9	7.2	6.077	469.1	3.1	0.303438
17	6.139	484	43.2	0.0678446	5	0.514351	2.8	2.3	6.139	482.9	4.6	0.0625224
18	6.172	492	43.9	0.102007	4	0.590304	3.2	2.6	6.172	491.1	3.8	0.0879039
19	6.418	552	49.2	0.0142934	2	0.0519068	0.3	0.2	6.418	552.4	2.1	0.0142758
20	6.454	561	50	0.0176617	3	0.0852571	0.5	0.4	6.454	561.1	2.8	0.0174542
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.512	575	51.3	0.0831383	3	0.4212	2.3	1.8	6.512	575.1	2.8	0.0861605
22	6.633	595	53.1	0.22726	5	1.97819	10.8	8.7	6.633	595.3	5.1	0.220409
23	6.704	604	53.9	0.154848	2	0.584462	3.2	2.6	6.704	604.4	2.4	0.138134
24	6.885	629	56.1	0.0500242	5	0.471569	2.6	2.1	6.885	629.1	5.3	0.0502282
25	7.719	784	69.9	0.0148781	7	0.19527	1.1	0.9	7.719	785	6.5	0.0168719
26	7.834	806	71.9	0.0801716	4	0.573334	3.1	2.5	7.834	805.9	4.1	0.0782054
27	8.823	1010	90.1	0.0686058	11	1.28443	7	5.6	8.823	1011.4	10.9	0.0663703

PeakCounter: 27

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	43	3.8	0.107153	10	1.86914	5.9	4.9	3.5	44.4	10.2	0.103541
2	3.766	81	7.2	0.0551863	6	0.591657	1.9	1.6	3.766	81.8	6.1	0.0549802
3	4.27	153	13.7	0.276893	4	2.02217	6.4	5.3	4.27	152.8	4.1	0.278506
4	4.431	176	15.7	0.027426	7	0.310324	1	0.8	4.431	176.6	6.6	0.0264565
5	4.55	193	17.2	0.114654	5	0.861357	2.7	2.3	4.55	192.9	4.7	0.104496
6	4.658	206	18.4	0.0380023	6	0.324076	1	0.9	4.658	204.9	5.7	0.0320546
7	4.808	224	20	0.0150627	5	0.106408	0.3	0.3	4.808	225.6	4.9	0.0121944
8	4.95	241	21.5	0.0465374	3	0.267479	0.8	0.7	4.95	241.3	3.3	0.0460469
9	5.075	256	22.9	0.0479449	5	0.327767	1	0.9	5.075	256.1	4.5	0.0410926
10	5.2	271	24.2	0.139903	5	1.09135	3.4	2.9	5.2	271	4.7	0.130531
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.308	295	26.3	0.189283	3	0.82822	2.6	2.2	5.308	295.4	2.6	0.181957
12	5.63	367	32.8	0.261055	4	1.66926	5.3	4.4	5.63	367.3	3.6	0.264147
13	5.769	398	35.5	0.0636072	5	0.519525	1.6	1.4	5.769	398	5.3	0.0551954
14	5.85	416	37.1	0.220986	3	1.10585	3.5	2.9	5.85	415.7	2.8	0.222287
15	5.978	447	39.9	0.429493	3	2.43304	7.7	6.4	5.978	447	3.1	0.439158
16	6.065	468	41.8	0.293763	3	1.58344	5	4.2	6.065	468.2	3.2	0.281963
17	6.122	482	43	0.0472632	7	0.67507	2.1	1.8	6.122	484	7.3	0.0525256
18	6.159	491	43.8	0.0879075	2	0.302358	1	0.8	6.159	492.1	2.5	0.068508
19	6.436	558	49.8	0.0147243	3	0.0793639	0.3	0.2	6.436	556.9	2.9	0.0155299
20	6.461	564	50.4	0.0176099	4	0.1189	0.4	0.3	6.461	564.6	3.8	0.0174879
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.531	581	51.9	0.106113	4	0.857448	2.7	2.3	6.531	580.2	4.5	0.107708
22	6.664	600	53.6	0.208921	5	1.96328	6.2	5.2	6.664	601.4	5.2	0.212857
23	6.744	610	54.5	0.232668	1	0.568693	1.8	1.5	6.744	610.2	1.5	0.216476
24	6.808	618	55.2	0.0341469	5	0.290624	0.9	0.8	6.808	619.1	5	0.0330978
25	6.909	634	56.6	0.0449636	5	0.395163	1.2	1	6.909	635.1	4.9	0.0455485
26	7.35	713	63.7	0.157928	5	1.21251	3.8	3.2	7.35	712.1	4.6	0.149784
27	7.78	793	70.8	0.0378776	9	0.62126	2	1.6	7.78	791.1	9.3	0.0377001
28	7.887	813	72.6	0.0979934	5	0.87431	2.8	2.3	7.887	813.1	5.4	0.0912639
29	8.15	862	77	0.26079	6	1.97333	6.2	5.2	8.15	864.4	6	0.185526
30	8.45	934	83.4	0.082504	7	0.98581	3.1	2.6	8.45	934.1	6.9	0.0809513
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.65	977	87.2	0.0704803	5	0.605405	1.9	1.6	8.65	977.2	5	0.0679607
32	8.839	1014	90.5	0.040789	10	0.645401	2	1.7	8.839	1014.9	9.7	0.0376921
33	9.013	1048	93.6	0.022407	7	0.260242	0.8	0.7	9.013	1048.8	6.6	0.0221502
34	9.08	1061	94.7	0.0250497	7	0.229259	0.7	0.6	9.08	1061.8	7.1	0.0181026
35	9.3	1104	98.6	0.370846	5	3.1068	9.8	8.2	9.3	1102.5	5.2	0.33645

PeakCounter: 35

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	45	4	0.156609	12	3.1583	18.6	13.7	3.5	45.3	12.4	0.143996
2	4.55	200	17.9	0.133126	6	1.30346	7.7	5.7	4.55	198.8	6	0.121645
3	5.2	282	25.2	0.208645	5	1.87078	11	8.1	5.2	281.1	5.3	0.199292
4	5.85	424	37.9	0.306126	3	1.71643	10.1	7.5	5.85	423.8	3.2	0.307184
5	6.55	588	52.5	0.213261	4	1.41107	8.3	6.1	6.55	587.3	4	0.197074
6	6.85	625	55.8	0.0679676	6	0.614701	3.6	2.7	6.85	624.9	6	0.0581336
7	7.35	716	63.9	0.14956	4	1.14555	6.8	5	7.35	715.8	4.4	0.146433
8	8.15	874	78	0.0603461	9	0.939522	5.5	4.1	8.15	873.9	9.2	0.0573645
9	8.45	939	83.8	0.0833127	6	0.914661	5.4	4	8.45	939.6	6.3	0.082256
10	8.65	979	87.4	0.068663	6	0.671682	4	2.9	8.65	978.8	5.9	0.0646833
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1106	98.8	0.315433	6	3.21126	18.9	14	9.3	1104.9	6.3	0.289232

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK SP CO MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
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PeakCount: 124

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 14.400 4.550 8.000 5.200 9.400 5.850 9.100 6.550 7.700 6.850 3.400 7.350 6.800 8.150 5.700 8.450 5.000 8.650 4.200 9.300 26.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 18 LANE TYPE: pI Markers ACTUAL GEL LANE: 20 PeakCount: 11 Return to Lane Navigator
3.819 3.500 4.295 10.600 4.458 0.700 4.599 1.100 4.723 0.600 4.863 0.800 4.994 1.100 5.109 1.400 5.209 1.100 5.255 0.700 5.327 4.500 5.636 8.700 5.782 2.300 5.854 2.000 5.979 10.900 6.058 8.400 6.124 4.300 6.153 2.600 6.411 0.500 6.448 1.400 6.506 2.500 6.640 13.600 6.710 1.100 6.877 2.800 7.668 1.500 7.786 3.500 8.808 6.800 8.994 1.100 9.067 0.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 17 LANE TYPE: Specimen ACTUAL GEL LANE: 19 PeakCount: 29 Return to Lane Navigator
3.500 17.600 4.550 7.200 5.200 10.600 5.850 9.700 6.550 8.100 6.850 3.900 7.350 7.700 8.150 5.800 8.450 6.000 8.650 4.300 9.300 19.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator
3.807 3.900 4.314 11.400 4.476 0.900 4.637 1.200 4.736 1.000 4.894 1.100 5.034 1.200 5.159 1.200 5.245 0.300 5.281 1.800 5.362 4.500 5.668 9.300 5.812 2.400 5.881 2.100 6.004 12.200 6.077 9.000 6.139 2.800 6.172 3.200 6.418 0.300 6.454 0.500 6.512 2.300 6.633 10.800 6.704 3.200 6.885 2.600 7.719 1.100 7.834 3.100 8.823 7.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 27 Return to Lane Navigator
3.500 5.900 3.766 1.900 4.270 6.400 4.431 1.000 4.550 2.700 4.658 1.000 4.808 0.300 4.950 0.800 5.075 1.000 5.200 3.400 5.308 2.600 5.630 5.300 5.769 1.600 5.850 3.500 5.978 7.700 6.065 5.000 6.122 2.100 6.159 1.000 6.436 0.300 6.461 0.400 6.531 2.700 6.664 6.200 6.744 1.800 6.808 0.900 6.909 1.200 7.350 3.800 7.780 2.000 7.887 2.800 8.150 6.200 8.450 3.100 8.650 1.900 8.839 2.000 9.013 0.800 9.080 0.700 9.300 9.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 35 Return to Lane Navigator
3.500 18.600 4.550 7.700 5.200 11.000 5.850 10.100 6.550 8.300 6.850 3.600 7.350 6.800 8.150 5.500 8.450 5.400 8.650 4.000 9.300 18.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Silk Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Silk Snapper ---- Go to Display Gel Lane Navigator.