Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Sierra  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Scomberomorus sierra
FDA Seafood List Common Name: Pacific Sierra
FDA Seafood List Market Name: Mackerel, Spanish
Unique IEF Gel Code: 19971031gel01
Unique Web Code: srh01Srr
Tissue Aliquot Code: scomsier J97-003 #01a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.558910.40.073316950.6149898.24.84.5590.25.30.0655673
25.217520.40.045930560.44916963.55.2174.85.90.0429404
35.8528633.40.077507740.5378717.24.25.85285.63.90.0782147
46.5543851.20.089442271.0126913.57.96.55435.37.40.0774065
56.8546854.70.064399490.83080211.16.56.85469.490.0522463
67.3554163.20.21163331.2334516.49.77.355413.40.202839
78.1567378.60.081079870.94404812.67.48.15672.86.80.0787595
88.4573886.20.084825570.94720712.67.48.45737.66.60.0810197
98.6578791.90.070374550.6320938.458.65786.15.10.0698777
109.3839980.046057640.3053644.12.49.3838.33.80.0452427
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.024242.80.22627362.516028.47.74.02423.46.30.224275
24.84312614.70.26952862.723929.18.44.843125.26.20.246454
35.00314617.10.061500350.4985341.71.55.003144.74.90.0573356
45.08415618.20.034396150.2590680.90.85.084156.94.60.0317657
55.2517920.90.017839370.2112590.70.65.25176.87.30.0162509
65.34319522.80.025506750.1998990.70.65.343193.850.0227302
75.45921525.10.010637850.1010540.30.35.459215.85.40.0106426
85.72826130.50.028654440.1778390.60.55.728261.63.60.0280159
96.11734139.80.33979142.0852876.46.117341.23.50.334501
106.1935741.70.054838330.2547610.90.86.19356.72.60.0542476
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.22636542.60.096352740.7281282.42.26.226365.84.20.0966945
126.30338244.60.063328860.6150212.11.96.303382.65.80.0599694
136.46641848.80.0461748110.806262.72.56.466420.211.30.0401585
146.636445520.1492220.59999721.86.636445.62.20.154087
156.70845252.80.22072541.422714.84.46.708452.140.202803
166.809462540.19030320.7321322.52.26.8094622.30.183587
176.89147255.10.021941430.1307250.40.46.891471.73.30.0223101
187.00548957.10.43445753.6916212.411.37.005488.64.80.434717
197.06649858.20.12412520.5065621.71.67.066497.82.30.122581
207.14150959.50.042916830.1866690.60.67.141508.62.80.0377727
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.19551760.40.059729350.5071951.71.67.195516.350.0572922
227.3554063.10.40402242.570538.67.97.35539.83.60.402188
238.54175888.60.143904112.465928.37.68.541761.910.80.12903
248.60677490.40.34122931.52295.14.78.606773.93.20.271401
258.77979592.90.103637183.1244610.59.68.779795.2180.0979344
269.33283897.90.15507741.136933.83.59.332837.64.40.146587
PeakCounter: 26
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.559210.70.057492360.5376798.76.44.5593.55.70.0531504
25.216819.60.031604360.3301115.43.95.2169.86.10.0304093
35.8528032.70.055593450.5267728.66.35.85279.35.40.0547968
46.5543751.10.11271771.229662014.76.55435.86.50.106029
56.8546654.40.02164380.262914.33.16.85468.27.60.0193993
67.3554163.20.039146370.3941496.44.77.35540.76.70.0333452
78.1567078.30.079765670.99923716.211.98.15668.97.50.0755957
88.4573585.90.078172270.89498914.510.78.45735.36.60.0764235
98.6578591.70.076359640.5520996.68.65784.64.20.0739805
109.383497.40.04760150.4235686.95.19.3833.95.10.046439
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.005273.20.14123861.403416.35.84.00526.55.60.140699
24.559110.70.037095160.3325011.51.44.5592.65.70.033046
34.79712014.10.1759661.807928.17.54.797121.460.170552
44.99314316.70.027135450.22750910.94.993141.54.80.0269569
55.07815317.90.012824930.06083140.30.35.078153.83.10.0109808
65.19716719.60.02170860.21896110.95.197167.560.0206689
75.36119222.50.010288440.07194880.30.35.361192.74.30.0093713
85.76525429.70.018422230.09674040.40.45.765253.73.10.0173895
95.8526731.30.039055430.2260510.95.85267.43.10.0408985
106.16234039.80.28658931.536836.96.46.162340.23.10.277089
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.23835841.90.044191520.1788460.80.76.238358.62.30.0443569
126.28136843.10.076945440.5491412.52.36.281367.94.30.0720321
136.349384450.050369250.43903321.86.349384.15.30.0466208
146.4941748.80.025789540.1653450.70.76.49416.83.90.0236288
156.52942649.90.030799550.2519541.116.529426.74.70.0302684
166.71344852.50.1579761.529866.96.36.713447.15.50.156804
176.80945853.60.17251441.219135.556.809458.54.30.158771
186.96848056.20.43864532.7066712.211.26.968479.63.40.454449
197.02148857.10.081942930.5227042.42.27.021488.93.40.0873193
207.14150659.30.035954560.3925881.81.67.141504.26.30.0349601
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.30153062.10.37820632.2443110.19.37.3015303.40.374981
228.1566377.60.0445203100.8515833.83.58.15659.69.60.0501675
238.4573185.60.046848140.09157440.40.48.45728.74.30.0121556
248.574487.10.0689989171.935358.788.574417.40.0628127
258.57776489.50.2672341.613127.36.78.577764.13.60.250302
268.6578391.70.063595770.6229072.82.68.65784.46.60.0535977
279.383497.70.12928340.9351554.23.99.3833.94.10.128178
PeakCounter: 27
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.0362630.1890872.18338.37.64.03625.56.60.186712
24.85212614.70.21951672.6942610.39.44.852124.87.10.215446
35.02314717.20.048788150.4413231.71.55.023145.45.10.0488091
45.09715618.20.037540540.26650110.95.097157.44.40.0344478
55.274180210.015082980.1894750.70.75.2741787.70.0138256
65.39119823.10.02459950.1734180.70.65.391196.64.50.0215709
75.74925329.60.034916530.1775830.70.65.749253.32.90.0341347
86.139334390.30836131.889977.26.66.139333.83.50.306528
96.21935241.10.050725130.2306050.90.86.219351.62.80.0471169
106.25436042.10.10863640.8242813.12.96.254360.24.40.106322
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.31637443.70.078301250.6662922.52.36.316374.150.0754339
126.46640847.70.045835270.5261721.86.466407.27.40.0399573
136.5141848.80.024792730.09309290.40.36.51417.82.70.0196346
146.64843851.20.11336520.4383541.71.56.648436.71.90.129786
156.69344351.80.20322641.328015.14.66.693443.53.90.194527
166.791454530.20466430.882583.43.16.791454.22.70.185093
176.972479560.44757843.2828812.511.46.972478.640.466507
187.02648756.90.15251940.9205653.53.27.026487.63.50.147258
197.146505590.093118481.130964.33.97.146502.57.80.0821822
207.29252761.60.4351832.476139.58.67.292527.33.20.429917
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.51574887.40.112597102.012457.778.515750.810.40.109494
228.57776489.30.2919131.44515.558.577763.430.274165
238.64878291.40.0498678100.79720232.88.648785.19.90.0453353
249.33383597.50.15436341.104724.23.89.333834.34.10.15186
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.558910.40.061477560.5571469.26.74.5590.75.60.0557083
25.2172200.040274360.3850186.44.65.2172.35.80.0372385
35.8527231.70.067724450.5377278.96.45.85271.94.50.0671843
46.5542549.50.085473181.201619.814.46.55423.28.10.0836669
56.8546153.70.020439750.1601072.61.96.85460.64.80.0188466
67.3553662.50.054585850.4758987.95.77.35535.95.50.0491046
78.1566677.60.072084580.97494116.111.68.15665.27.90.069455
88.4573485.50.066925970.790149139.48.45732.46.60.0675583
98.6578491.40.068608940.4880458.15.88.65783.94.10.0665302
109.383397.10.055038850.4861685.89.38335.20.052317
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 107
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 26 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 27 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln