RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Skipjack Tuna ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Katsuwonus pelamis
FDA Seafood List Common Name: Skipjack Tuna
FDA Seafood List Market Name: Tuna
Unique IEF Gel Code: 19970129gel04
Unique Web Code: skh01Srr
Tissue Aliquot Code: katspela E96-002 #01a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	31	3.9	0.117016	5	0.936894	6.2	4.8	4.55	31.3	4.7	0.111586
2	5.2	119	15.1	0.173953	4	1.20183	8	6.1	5.2	118.5	3.9	0.171872
3	5.85	214	27.2	0.140579	4	1.03969	6.9	5.3	5.85	214.3	4.2	0.138585
4	6.55	350	44.5	0.131997	11	2.40742	16	12.2	6.55	348.6	10.9	0.124663
5	6.85	387	49.2	0.0515094	6	0.527421	3.5	2.7	6.85	387.1	6.4	0.046687
6	7.35	454	57.7	0.0747827	5	0.64067	4.3	3.3	7.35	453.9	5	0.0723568
7	8.15	584	74.2	0.160412	7	1.99157	13.2	10.1	8.15	583.7	7.2	0.156022
8	8.45	645	82	0.213503	8	2.9313	19.5	14.9	8.45	644.2	8	0.207828
9	8.65	702	89.2	0.148005	5	1.1407	7.6	5.8	8.65	701.3	4.6	0.14137
10	9.3	769	97.7	0.240738	6	2.24817	14.9	11.4	9.3	767.8	5.6	0.224763

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.43	14	1.8	0.0261107	5	0.232767	0.6	0.5	4.43	13.7	5.1	0.0255074
2	5.061	98	12.5	0.304978	4	2.34352	5.7	4.7	5.061	98.5	4.4	0.303838
3	5.443	152	19.3	0.0378038	8	0.471417	1.2	0.9	5.443	153.2	8.1	0.0327044
4	5.566	170	21.6	0.0349775	6	0.341089	0.8	0.7	5.566	169.7	5.8	0.032976
5	6.487	338	43	0.0982741	4	0.730716	1.8	1.5	6.487	337.4	4.3	0.0964263
6	6.527	346	44	0.0506349	3	0.296166	0.7	0.6	6.527	346.1	3.3	0.0504618
7	6.603	357	45.4	0.155913	2	0.532835	1.3	1.1	6.603	355.7	2.3	0.130563
8	6.635	361	45.9	0.158811	4	1.00156	2.4	2	6.635	360.6	3.7	0.154286
9	6.692	368	46.8	0.468199	2	1.89649	4.6	3.8	6.692	367.7	2.3	0.471855
10	6.781	379	48.2	0.131238	3	0.605296	1.5	1.2	6.781	378.6	2.6	0.131585
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.896	394	50.1	0.428491	3	2.15057	5.3	4.3	6.896	393.9	2.8	0.435159
12	7.025	412	52.4	0.099064	5	0.820146	2	1.6	7.025	412.1	4.9	0.0948087
13	7.111	424	53.9	0.164431	5	1.51628	3.7	3	7.111	425.7	5	0.172493
14	7.182	434	55.2	0.473135	5	3.8299	9.4	7.7	7.182	434	4.6	0.473428
15	7.268	446	56.7	0.0497639	3	0.25904	0.6	0.5	7.268	446.9	2.7	0.0532937
16	7.372	461	58.7	0.249693	5	2.1894	5.4	4.4	7.372	460.9	4.8	0.255635
17	7.492	480	61.1	0.260055	3	1.47306	3.6	3	7.492	479.8	3.2	0.259532
18	7.637	503	64	0.329387	3	1.96307	4.8	3.9	7.637	502.8	3.3	0.335052
19	7.983	558	71	0.473606	9	5.65653	13.8	11.3	7.983	555.3	9	0.353004
20	8.347	624	79.4	0.107499	10	1.84552	4.5	3.7	8.347	624	10.1	0.103227
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.398	634	80.7	0.107939	4	0.533939	1.3	1.1	8.398	635	4	0.0745932
22	8.518	664	84.5	0.0810446	4	0.247735	0.6	0.5	8.518	663.7	4.5	0.0312874
23	8.568	678	86.3	0.140987	13	2.56781	6.3	5.1	8.568	675.9	13	0.111049
24	8.714	708	90.1	0.195641	18	5.83952	14.3	11.7	8.714	705.9	18	0.183036
25	8.958	733	93.3	0.0675751	3	0.0700787	0.2	0.1	8.958	735.4	2.6	0.0149656
26	9.232	761	96.8	0.209421	4	1.48445	3.6	3	9.232	760	4	0.211971

PeakCounter: 26

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	28	3.6	0.0928547	5	0.868599	5.7	4.5	4.55	28.8	5.4	0.0905001
2	5.2	113	14.4	0.11393	5	1.09374	7.2	5.6	5.2	113.3	5.4	0.113988
3	5.85	208	26.6	0.135532	5	1.09291	7.2	5.6	5.85	208.1	4.7	0.131916
4	6.55	350	44.7	0.136485	11	2.5413	16.6	13.1	6.55	348.3	11.2	0.128215
5	6.85	387	49.4	0.034347	6	0.329005	2.2	1.7	6.85	388.2	6	0.031116
6	7.35	460	58.7	0.0559567	5	0.502634	3.3	2.6	7.35	459.1	5.3	0.053886
7	8.15	584	74.6	0.114645	12	2.166	14.2	11.1	8.15	585.5	12.2	0.100513
8	8.45	643	82.1	0.224949	8	3.10094	20.3	16	8.45	641.6	8.2	0.212665
9	8.65	700	89.4	0.146407	4	1.09273	7.2	5.6	8.65	699.1	4.3	0.144127
10	9.3	766	97.8	0.245015	6	2.49381	16.3	12.8	9.3	764.4	6.1	0.231001

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.318	9	1.1	0.0122627	5	0.107722	0.4	0.3	4.318	11.1	5.2	0.0116653
2	4.55	39	5	0.0720975	4	0.52109	1.7	1.6	4.55	39.5	4.2	0.0706406
3	4.999	97	12.4	0.169943	4	1.34448	4.4	4.1	4.999	96.8	4.4	0.172466
4	5.2	123	15.7	0.0848408	4	0.52518	1.7	1.6	5.2	122.4	3.6	0.0831052
5	5.375	148	18.9	0.0112065	9	0.191449	0.6	0.6	5.375	149.4	9	0.0120407
6	5.501	166	21.2	0.0219333	5	0.199904	0.7	0.6	5.501	166	5	0.0223667
7	5.85	216	27.6	0.0704794	4	0.534548	1.7	1.6	5.85	216.6	4.1	0.0727053
8	6.469	339	43.2	0.047617	8	0.822618	2.7	2.5	6.469	343.5	7.9	0.0586055
9	6.53	351	44.8	0.0665207	3	0.208204	0.7	0.6	6.53	350.9	2.9	0.040657
10	6.601	361	46	0.0917338	2	0.293407	1	0.9	6.601	360.6	2.1	0.0798216
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.643	366	46.7	0.112483	3	0.589666	1.9	1.8	6.643	365.8	3.1	0.106212
12	6.703	373	47.6	0.517186	2	1.91671	6.3	5.9	6.703	372.5	2.1	0.527339
13	6.779	382	48.7	0.0965854	2	0.367257	1.2	1.1	6.779	382.3	2.1	0.0982059
14	6.884	395	50.4	0.379565	3	1.92755	6.3	5.9	6.884	395.4	2.8	0.392378
15	7.015	413	52.7	0.0974027	4	0.58606	1.9	1.8	7.015	412.1	3.8	0.0870846
16	7.095	424	54.1	0.129442	9	2.43746	8	7.5	7.095	428.1	9.5	0.145052
17	7.19	437	55.7	0.431182	4	2.40204	7.8	7.4	7.19	437.2	3.6	0.375874
18	7.363	461	58.8	0.195687	5	1.73167	5.6	5.3	7.363	460.9	5.1	0.191979
19	7.49	481	61.4	0.219295	3	1.3125	4.3	4	7.49	480.9	3.4	0.214888
20	7.617	501	63.9	0.287485	3	1.67776	5.5	5.2	7.617	501.4	3.2	0.293889
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.896	545	69.5	0.0647088	2	0.260859	0.9	0.8	7.896	545.3	2.3	0.0647752
22	7.991	560	71.4	0.485716	3	2.84801	9.3	8.7	7.991	560.7	3.2	0.496382
23	8.15	585	74.6	0.0728261	9	1.04563	3.4	3.2	8.15	583.6	8.6	0.0686584
24	8.304	620	79.1	0.0186924	5	0.153338	0.5	0.5	8.304	619.4	5	0.0173083
25	8.353	631	80.5	0.0111734	3	0.0504614	0.2	0.2	8.353	630.9	3.1	0.0093253
26	8.45	653	83.3	0.0222035	6	0.227829	0.7	0.7	8.45	652.1	5.8	0.0222549
27	8.546	678	86.5	0.0479254	12	0.880533	2.9	2.7	8.546	679.9	12.2	0.0407632
28	8.65	705	89.9	0.135171	14	2.9959	9.8	9.2	8.65	706.3	13.6	0.123935
29	8.945	733	93.5	0.0532566	8	0.662812	2.2	2	8.945	731.8	8.1	0.0460508
30	9.219	759	96.8	0.186362	6	1.82721	6	5.6	9.219	759.9	5.8	0.179062

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.346	9	1.2	0.022623	6	0.215527	0.5	0.4	4.346	9.9	5.6	0.0218295
2	4.981	93	11.9	0.219921	6	2.28717	5.3	4.4	4.981	93.3	5.8	0.222253
3	5.366	146	18.7	0.0326656	9	0.495178	1.2	0.9	5.366	144.2	8.9	0.0313068
4	5.47	161	20.6	0.0277682	9	0.431352	1	0.8	5.47	162.9	9	0.027163
5	6.456	339	43.4	0.0893507	7	1.15737	2.7	2.2	6.456	341.3	7.4	0.0886353
6	6.55	358	45.8	0.124678	2	0.565644	1.3	1.1	6.55	359.2	2.4	0.135635
7	6.612	365	46.7	0.181828	4	1.17069	2.7	2.2	6.612	364.3	3.7	0.179661
8	6.666	371	47.4	0.528334	2	2.13827	5	4.1	6.666	371.3	2.3	0.535766
9	6.755	381	48.7	0.154467	3	0.686175	1.6	1.3	6.755	380.5	2.6	0.149405
10	6.86	393	50.3	0.439311	3	2.36789	5.5	4.5	6.86	393.3	3	0.440799
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.985	409	52.3	0.12225	10	2.18364	5.1	4.2	6.985	416.2	10.4	0.118853
12	7.132	428	54.7	0.450629	5	3.68562	8.6	7.1	7.132	428.4	5.1	0.408071
13	7.226	440	56.3	0.0195882	3	0.0994726	0.2	0.2	7.226	440.6	2.8	0.0202977
14	7.35	456	58.3	0.215067	5	1.97066	4.6	3.8	7.35	455.5	5.1	0.218043
15	7.481	477	61	0.206628	4	1.34262	3.1	2.6	7.481	476.8	3.6	0.208267
16	7.568	491	62.8	0.0279871	3	0.137611	0.3	0.3	7.568	490.9	2.9	0.0263485
17	7.636	502	64.2	0.246404	3	1.57314	3.7	3	7.636	502.2	3.5	0.253869
18	7.979	557	71.2	0.44028	8	4.94167	11.5	9.5	7.979	555.1	7.7	0.363182
19	8.34	625	79.9	0.114733	8	1.43781	3.4	2.8	8.34	621.6	8.3	0.097634
20	8.373	632	80.8	0.149161	7	1.53384	3.6	2.9	8.373	633.3	6.9	0.124622
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.514	666	85.2	0.106258	7	0.653323	1.5	1.3	8.514	661.2	6.7	0.0548457
22	8.558	678	86.7	0.161938	12	2.35138	5.5	4.5	8.558	674.9	11.8	0.112786
23	8.671	705	90.2	0.214732	18	6.54988	15.3	12.6	8.671	703.4	18	0.205302
24	8.963	733	93.7	0.0959032	10	1.1661	2.7	2.2	8.963	732.3	10.2	0.0646496
25	9.234	759	97.1	0.247852	4	1.71037	4	3.3	9.234	758.5	3.9	0.250076

PeakCounter: 25

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	30	3.9	0.125575	5	0.999031	6	4.8	4.55	30	4.7	0.12044
2	5.2	118	15.2	0.179442	4	1.36327	8.2	6.5	5.2	117.5	4.4	0.174892
3	5.85	213	27.4	0.158741	4	1.1726	7.1	5.6	5.85	213.1	4.3	0.155057
4	6.55	358	46	0.11601	12	2.38692	14.4	11.4	6.55	352.9	11.9	0.112761
5	6.85	390	50.1	0.0430357	6	0.477973	2.9	2.3	6.85	390.9	6.4	0.042064
6	7.35	450	57.8	0.0715349	5	0.591807	3.6	2.8	7.35	450.2	4.8	0.0696869
7	8.15	581	74.7	0.147516	10	2.39843	14.5	11.4	8.15	581.3	10.4	0.129766
8	8.45	641	82.4	0.191574	10	3.34932	20.2	16	8.45	639.8	10.2	0.185336
9	8.65	698	89.7	0.15952	4	1.1964	7.2	5.7	8.65	697.1	4.3	0.155196
10	9.3	761	97.8	0.259153	6	2.6179	15.8	12.5	9.3	759.9	6.1	0.243733

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 111

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 6.200 5.200 8.000 5.850 6.900 6.550 16.000 6.850 3.500 7.350 4.300 8.150 13.200 8.450 19.500 8.650 7.600 9.300 14.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.430 0.600 5.061 5.700 5.443 1.200 5.566 0.800 6.487 1.800 6.527 0.700 6.603 1.300 6.635 2.400 6.692 4.600 6.781 1.500 6.896 5.300 7.025 2.000 7.111 3.700 7.182 9.400 7.268 0.600 7.372 5.400 7.492 3.600 7.637 4.800 7.983 13.800 8.347 4.500 8.398 1.300 8.518 0.600 8.568 6.300 8.714 14.300 8.958 0.200 9.232 3.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 26 Return to Lane Navigator
4.550 5.700 5.200 7.200 5.850 7.200 6.550 16.600 6.850 2.200 7.350 3.300 8.150 14.200 8.450 20.300 8.650 7.200 9.300 16.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.318 0.400 4.550 1.700 4.999 4.400 5.200 1.700 5.375 0.600 5.501 0.700 5.850 1.700 6.469 2.700 6.530 0.700 6.601 1.000 6.643 1.900 6.703 6.300 6.779 1.200 6.884 6.300 7.015 1.900 7.095 8.000 7.190 7.800 7.363 5.600 7.490 4.300 7.617 5.500 7.896 0.900 7.991 9.300 8.150 3.400 8.304 0.500 8.353 0.200 8.450 0.700 8.546 2.900 8.650 9.800 8.945 2.200 9.219 6.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 30 Return to Lane Navigator
4.346 0.500 4.981 5.300 5.366 1.200 5.470 1.000 6.456 2.700 6.550 1.300 6.612 2.700 6.666 5.000 6.755 1.600 6.860 5.500 6.985 5.100 7.132 8.600 7.226 0.200 7.350 4.600 7.481 3.100 7.568 0.300 7.636 3.700 7.979 11.500 8.340 3.400 8.373 3.600 8.514 1.500 8.558 5.500 8.671 15.300 8.963 2.700 9.234 4.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 25 Return to Lane Navigator
4.550 6.000 5.200 8.200 5.850 7.100 6.550 14.400 6.850 2.900 7.350 3.600 8.150 14.500 8.450 20.200 8.650 7.200 9.300 15.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Skipjack Tuna ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Skipjack Tuna ---- Go to Display Gel Lane Navigator.