RFE Fish Species Identification Isoelectric Focusing Tools - Pacific Ocean Perch PAGPlate IEF Gel 01

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Ocean Perch ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sebastes alutus
FDA Seafood List Common Name: Pacific Ocean Perch
FDA Seafood List Market Name: Perch, Ocean
Unique IEF Gel Code: 19980831gel02
Unique Web Code: ppg01Srr
Tissue Aliquot Code: sebaalut I94-001 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	49	2.2	0.398213	16	10.8078	9.5	6.7	3.5	53.6	16.4	0.37081
2	4.55	337	15.4	0.536906	6	5.06585	4.5	3.1	4.55	337.3	5.9	0.487456
3	5.2	511	23.4	2.35169	3	11.739	10.4	7.2	5.2	511	3	2.2098
4	5.85	821	37.5	2.39266	4	18.4943	16.3	11.4	5.85	822.9	3.9	2.68098
5	6.55	1159	53	0.823659	6	7.24984	6.4	4.5	6.55	1158.2	5.6	0.736608
6	6.85	1245	56.9	0.558186	8	7.44404	6.6	4.6	6.85	1244.2	8.3	0.50384
7	7.35	1431	65.4	1.43767	6	12.413	11	7.7	7.35	1429.5	5.5	1.26347
8	8.15	1740	79.6	0.175383	15	4.38736	3.9	2.7	8.15	1740.2	14.5	0.170532
9	8.45	1861	85.1	0.279384	10	4.95785	4.4	3.1	8.45	1861.7	10.2	0.273597
10	8.65	1937	88.6	0.23663	9	3.76469	3.3	2.3	8.65	1936.9	9.3	0.228354
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2164	98.9	2.18612	6	27.0293	23.8	16.7	9.3	2166.7	6.2	2.45762

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 17
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.139	226	10.3	2.36818	7	34.5965	26.1	23.9	4.139	229.2	7.5	2.60475
2	4.406	300	13.7	0.0832746	11	1.63438	1.2	1.1	4.406	299.2	11.4	0.0806681
3	4.489	323	14.8	0.0910989	8	1.39006	1	1	4.489	323.7	8.3	0.0949406
4	4.75	393	18	1.78302	10	31.4867	23.7	21.7	4.75	392.3	10.1	1.75073
5	4.897	432	19.8	0.0318647	6	0.364028	0.3	0.3	4.897	431.4	6.3	0.0328515
6	5.055	474	21.7	0.107795	12	2.17667	1.6	1.5	5.055	472.3	12	0.102564
7	5.123	492	22.5	0.0563744	7	0.596143	0.4	0.4	5.123	492.5	7	0.0478705
8	5.203	514	23.5	0.0370333	8	0.560619	0.4	0.4	5.203	510.2	8.2	0.038564
9	5.235	529	24.2	0.0118168	7	0.208905	0.2	0.1	5.235	535	7.4	0.0158436
10	5.337	578	26.4	0.0281234	15	0.706913	0.5	0.5	5.337	582.8	14.8	0.0269171
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.695	749	34.2	0.161493	9	2.02527	1.5	1.4	5.695	746.4	8.9	0.129033
12	5.716	759	34.7	0.140325	11	2.40116	1.8	1.7	5.716	761.3	11.1	0.122514
13	5.781	790	36.1	0.0636134	5	0.51278	0.4	0.4	5.781	789.4	4.9	0.0590856
14	5.848	822	37.6	2.21771	6	26.7752	20.2	18.5	5.848	825	6.3	2.41319
15	5.949	871	39.8	0.259282	8	3.5361	2.7	2.4	5.949	871.8	7.8	0.254225
16	6.005	898	41.1	0.320691	5	2.40184	1.8	1.7	6.005	898.7	5.3	0.256636
17	6.061	925	42.3	0.138662	24	5.91291	4.5	4.1	6.061	921.6	24.1	0.138639
18	6.105	946	43.3	0.0627425	1	0	0	0	6.105	940	1	0
19	6.13	958	43.8	0.22809	2	0.688652	0.5	0.5	6.13	957.9	2.1	0.182473
20	6.167	976	44.6	2.28389	2	10.3959	7.8	7.2	6.167	976.2	2.4	2.45818
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.233	1008	46.1	0.113854	9	1.84688	1.4	1.3	6.233	1007.8	9.4	0.110334
22	6.366	1072	49	0.0404955	10	0.716373	0.5	0.5	6.366	1074.9	9.6	0.0423037
23	6.488	1131	51.7	0.0326601	13	0.705535	0.5	0.5	6.488	1131.7	13.1	0.0304914
24	7.643	1544	70.6	0.0408887	14	0.96924	0.7	0.7	7.643	1542.6	13.8	0.0395134

PeakCounter: 24

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 18
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 19
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	49	2.2	0.380543	13	8.50665	9.2	6.9	3.5	50	13.2	0.364538
2	4.55	343	15.7	0.530454	6	4.95426	5.4	4	4.55	343.8	5.7	0.489596
3	5.2	514	23.5	1.83729	3	9.54354	10.3	7.8	5.2	513.7	3.3	1.64605
4	5.85	825	37.7	2.39179	4	18.3273	19.8	14.9	5.85	823.5	3.9	2.64371
5	6.55	1163	53.2	0.917572	5	7.4369	8	6.1	6.55	1162.9	5.1	0.824196
6	6.85	1248	57.1	0.520593	8	6.74171	7.3	5.5	6.85	1247.7	8	0.474337
7	7.35	1431	65.5	0.954484	7	10.1245	11	8.2	7.35	1429.6	6.8	0.843145
8	8.15	1739	79.6	0.150745	15	3.96052	4.3	3.2	8.15	1737	15.2	0.146756
9	8.45	1857	84.9	0.270016	10	4.69203	5.1	3.8	8.45	1857.3	10	0.263776
10	8.65	1931	88.3	0.23876	9	3.82011	4.1	3.1	8.65	1930.5	9.2	0.233321
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2166	99.1	1.77122	5	14.3421	15.5	11.7	9.3	2165.4	5	1.61339

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 19
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 20
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	47	2.2	0.353948	15	9.09332	4.7	4.4	3.5	46.1	15.5	0.331302
2	4.089	223	10.2	2.3644	7	32.7104	17	15.8	4.089	227.1	7.1	2.60834
3	4.349	301	13.8	0.0930492	11	1.64102	0.9	0.8	4.349	300.8	10.9	0.08527
4	4.433	326	14.9	0.120453	7	1.56496	0.8	0.8	4.433	325.4	7.4	0.118773
5	4.55	361	16.5	0.247137	8	3.64874	1.9	1.8	4.55	361	8.5	0.243525
6	4.665	391	17.9	2.19452	8	29.3608	15.2	14.2	4.665	393.2	8.2	2.0305
7	4.821	432	19.8	0.0440208	6	0.458549	0.2	0.2	4.821	431.6	5.8	0.0449223
8	4.971	471	21.6	0.159166	8	2.14344	1.1	1	4.971	470.2	8.2	0.147705
9	5.024	485	22.2	0.0751061	9	1.11896	0.6	0.5	5.024	486.3	8.8	0.0721003
10	5.097	504	23.1	0.0431701	9	0.67783	0.4	0.3	5.097	503.9	9.4	0.0405271
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.2	531	24.3	0.648762	7	7.23724	3.8	3.5	5.2	533.5	7	0.579522
12	5.314	583	26.7	0.0341947	7	0.374476	0.2	0.2	5.314	580.5	7.1	0.029788
13	5.331	591	27	0.0353647	8	0.469539	0.2	0.2	5.331	593	7.9	0.0334267
14	5.482	660	30.2	0.0265766	9	0.424882	0.2	0.2	5.482	658.5	9.1	0.0262521
15	5.568	699	32	0.0140661	3	0.0972173	0.1	0	5.568	697.3	3.4	0.0161353
16	5.605	716	32.8	0.012495	6	0.204308	0.1	0.1	5.605	712	5.7	0.0203976
17	5.681	751	34.4	0.118381	7	1.3466	0.7	0.6	5.681	751.6	7.4	0.102501
18	5.706	762	34.9	0.147759	7	1.81887	0.9	0.9	5.706	762.3	7.4	0.138341
19	5.76	787	36	0.0682311	5	0.601609	0.3	0.3	5.76	786.1	5.4	0.0629677
20	5.782	797	36.5	0.0695539	6	0.795054	0.4	0.4	5.782	796.6	6.5	0.0691112
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	5.85	828	37.9	2.20496	6	26.2555	13.6	12.7	5.85	830.9	6.1	2.4122
22	5.956	877	40.1	0.265994	9	4.60647	2.4	2.2	5.956	878.6	9.4	0.276577
23	6.014	904	41.4	0.24525	7	2.77084	1.4	1.3	6.014	904.3	6.9	0.225481
24	6.068	929	42.5	0.162631	16	4.63963	2.4	2.2	6.068	927	15.8	0.165814
25	6.118	952	43.6	0.0304014	1	0	0	0	6.118	946	1	0
26	6.146	965	44.2	0.104203	4	0.637472	0.3	0.3	6.146	964.6	3.6	0.0995625
27	6.189	985	45.1	1.00166	5	9.87452	5.1	4.8	6.189	982.7	5.1	1.08972
28	6.258	1017	46.5	0.0925495	12	1.88621	1	0.9	6.258	1017.5	12.1	0.0879153
29	6.412	1088	49.8	0.0467209	13	1.11291	0.6	0.5	6.412	1088.9	12.9	0.0486493
30	6.472	1116	51.1	0.0231629	8	0.31821	0.2	0.2	6.472	1113.1	8	0.0223763
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	6.55	1152	52.7	0.175346	13	4.37218	2.3	2.1	6.55	1153.5	13.5	0.182973
32	6.85	1249	57.2	0.230947	13	4.97129	2.6	2.4	6.85	1245.8	12.6	0.222884
33	7.35	1422	65.1	0.427399	11	8.48596	4.4	4.1	7.35	1422.7	11.2	0.428546
34	7.69	1554	71.1	0.0356564	14	0.914719	0.5	0.4	7.69	1554.1	14	0.0369169
35	8.15	1733	79.3	0.0976984	18	3.02098	1.6	1.5	8.15	1730.4	17.8	0.0959505
36	8.45	1863	85.3	0.17625	11	3.24651	1.7	1.6	8.45	1862.6	10.6	0.172904
37	8.65	1939	88.7	0.161743	9	2.44876	1.3	1.2	8.65	1939.1	8.7	0.158586
38	9.3	2168	99.2	2.24469	4	17.508	9.1	8.4	9.3	2167.3	4.4	2.26242

PeakCounter: 38

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 20
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 21
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.07	212	9.7	2.37708	7	30.8465	24.6	22.9	4.07	212.8	7	2.49693
2	4.307	282	12.9	0.077128	11	1.45461	1.2	1.1	4.307	282.3	11	0.0744698
3	4.385	305	14	0.0974906	8	1.31769	1.1	1	4.385	305.7	7.8	0.0956887
4	4.624	373	17.1	2.21234	8	30.6155	24.4	22.8	4.624	371.8	8.5	2.0395
5	4.748	406	18.6	0.0353949	6	0.379332	0.3	0.3	4.748	407.6	6.3	0.0339903
6	4.903	447	20.5	0.144691	8	1.64213	1.3	1.2	4.903	446.7	7.9	0.117346
7	5.009	475	21.7	0.0306507	15	1.572	1.3	1.2	5.009	461.7	15.3	0.0581328
8	5.066	490	22.4	0.0242335	7	0.289702	0.2	0.2	5.066	491	7.1	0.0230756
9	5.164	516	23.6	0.0155775	11	0.29957	0.2	0.2	5.164	517.4	10.6	0.0159855
10	5.283	564	25.8	0.0304563	14	0.745717	0.6	0.6	5.283	564.3	14.4	0.0292476
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.645	733	33.5	0.126174	7	1.44822	1.2	1.1	5.645	732.1	6.7	0.121457
12	5.669	744	34.1	0.173559	6	1.44352	1.2	1.1	5.669	744.1	6.2	0.13079
13	5.712	764	35	0.0580504	10	0.976288	0.8	0.7	5.712	762.9	10.3	0.0537369
14	5.733	774	35.4	0.0585635	4	0.346533	0.3	0.3	5.733	775.4	4.2	0.0460098
15	5.812	811	37.1	2.23933	6	24.8268	19.8	18.5	5.812	812.2	5.8	2.40568
16	5.851	829	37.9	0.110183	7	1.39028	1.1	1	5.851	829.1	7.1	0.110656
17	5.919	861	39.4	0.28154	10	4.67279	3.7	3.5	5.919	861.8	9.8	0.269989
18	5.977	888	40.6	0.270256	6	2.32577	1.9	1.7	5.977	889.3	5.8	0.224949
19	6.037	916	41.9	0.199337	19	6.9237	5.5	5.1	6.037	913.7	18.8	0.207949
20	6.082	937	42.9	0.00613317	1	0	0	0	6.082	878	1	0
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.131	960	43.9	0.096744	10	1.41672	1.1	1.1	6.131	962.4	9.9	0.0809247
22	6.163	975	44.6	1.88209	2	7.56385	6	5.6	6.163	974.4	2.4	1.80584
23	6.231	1007	46.1	0.0852012	10	1.51142	1.2	1.1	6.231	1006.1	10.1	0.0841222
24	6.365	1070	49	0.0354053	10	0.687869	0.5	0.5	6.365	1071.5	10.4	0.0374152
25	6.474	1121	51.3	0.0291048	10	0.484761	0.4	0.4	6.474	1124.4	9.8	0.0279993
26	7.665	1550	70.9	0.0298993	6	0.259655	0.2	0.2	7.665	1547.4	6.2	0.0235247

PeakCounter: 26

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 21
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 22
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	40	1.8	0.414872	13	8.83562	8.7	6.2	3.5	42.7	12.7	0.392692
2	4.55	345	15.8	0.471013	6	4.98876	4.9	3.5	4.55	345.1	6.4	0.44072
3	5.2	519	23.8	1.88731	4	10.9358	10.7	7.6	5.2	519.8	3.5	1.73961
4	5.85	828	37.9	2.30962	5	18.0939	17.8	12.6	5.85	827.7	4.6	2.19755
5	6.55	1160	53.1	0.842303	5	7.63291	7.5	5.3	6.55	1159.2	5.3	0.80575
6	6.85	1248	57.1	0.472888	9	7.18878	7.1	5	6.85	1247.5	9.4	0.430377
7	7.35	1437	65.8	1.71516	5	15.0341	14.8	10.5	7.35	1435.7	5.3	1.58881
8	8.15	1735	79.4	0.187504	15	4.77847	4.7	3.3	8.15	1734.4	15.1	0.178818
9	8.45	1858	85.1	0.267703	10	4.80392	4.7	3.3	8.45	1856.6	10.3	0.263493
10	8.65	1928	88.3	0.20499	10	3.46632	3.4	2.4	8.65	1926.9	9.8	0.199627
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	2153	98.6	2.27416	4	16.0303	15.7	11.2	9.3	2152.4	4.2	2.14874

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 121

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 9.500 4.550 4.500 5.200 10.400 5.850 16.300 6.550 6.400 6.850 6.600 7.350 11.000 8.150 3.900 8.450 4.400 8.650 3.300 9.300 23.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 17 PeakCount: 11 Return to Lane Navigator
4.139 26.100 4.406 1.200 4.489 1.000 4.750 23.700 4.897 0.300 5.055 1.600 5.123 0.400 5.203 0.400 5.235 0.200 5.337 0.500 5.695 1.500 5.716 1.800 5.781 0.400 5.848 20.200 5.949 2.700 6.005 1.800 6.061 4.500 6.105 0 6.130 0.500 6.167 7.800 6.233 1.400 6.366 0.500 6.488 0.500 7.643 0.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 17 LANE TYPE: Specimen ACTUAL GEL LANE: 18 PeakCount: 24 Return to Lane Navigator
3.500 9.200 4.550 5.400 5.200 10.300 5.850 19.800 6.550 8.000 6.850 7.300 7.350 11.000 8.150 4.300 8.450 5.100 8.650 4.100 9.300 15.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 18 LANE TYPE: pI Markers ACTUAL GEL LANE: 19 PeakCount: 11 Return to Lane Navigator
3.500 4.700 4.089 17.000 4.349 0.900 4.433 0.800 4.550 1.900 4.665 15.200 4.821 0.200 4.971 1.100 5.024 0.600 5.097 0.400 5.200 3.800 5.314 0.200 5.331 0.200 5.482 0.200 5.568 0.100 5.605 0.100 5.681 0.700 5.706 0.900 5.760 0.300 5.782 0.400 5.850 13.600 5.956 2.400 6.014 1.400 6.068 2.400 6.118 0 6.146 0.300 6.189 5.100 6.258 1.000 6.412 0.600 6.472 0.200 6.550 2.300 6.850 2.600 7.350 4.400 7.690 0.500 8.150 1.600 8.450 1.700 8.650 1.300 9.300 9.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 19 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 20 PeakCount: 38 Return to Lane Navigator
4.070 24.600 4.307 1.200 4.385 1.100 4.624 24.400 4.748 0.300 4.903 1.300 5.009 1.300 5.066 0.200 5.164 0.200 5.283 0.600 5.645 1.200 5.669 1.200 5.712 0.800 5.733 0.300 5.812 19.800 5.851 1.100 5.919 3.700 5.977 1.900 6.037 5.500 6.082 0 6.131 1.100 6.163 6.000 6.231 1.200 6.365 0.500 6.474 0.400 7.665 0.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 20 LANE TYPE: Specimen ACTUAL GEL LANE: 21 PeakCount: 26 Return to Lane Navigator
3.500 8.700 4.550 4.900 5.200 10.700 5.850 17.800 6.550 7.500 6.850 7.100 7.350 14.800 8.150 4.700 8.450 4.700 8.650 3.400 9.300 15.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 21 LANE TYPE: pI Markers ACTUAL GEL LANE: 22 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Pacific Ocean Perch ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Pacific Ocean Perch ---- Go to Display Gel Lane Navigator.