RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Oilfish(*) ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Ruvettus(*) pretiosus(*)
FDA Seafood List Common Name: Oilfish(*)
FDA Seafood List Market Name: Oilfish(*)
Unique IEF Gel Code: 19970424gel04
Unique Web Code: oih04Srr
Tissue Aliquot Code: ruvepret H95-002 #06m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	26	3.3	0.0713882	4	0.474582	5.7	4.3	4.55	25.9	3.9	0.0689548
2	5.2	119	15.3	0.0793268	5	0.674005	8.1	6.2	5.2	119.2	5	0.0765226
3	5.85	236	30.3	0.103647	4	0.709247	8.6	6.5	5.85	235.7	3.9	0.102191
4	6.55	379	48.7	0.121427	7	1.3665	16.5	12.5	6.55	378.8	6.9	0.111761
5	6.85	414	53.2	0.0476646	7	0.531373	6.4	4.9	6.85	415	6.8	0.0437745
6	7.35	477	61.3	0.0602989	5	0.534712	6.5	4.9	7.35	477.2	5.2	0.0574965
7	8.15	615	79	0.0728381	11	1.38125	16.7	12.6	8.15	613.6	10.7	0.0729879
8	8.45	679	87.3	0.131701	7	1.65539	20	15.1	8.45	678.7	7.4	0.126106
9	8.65	724	93.1	0.109021	5	0.881591	10.6	8.1	8.65	724	4.8	0.104216
10	9.3	765	98.3	0.0155222	3	0.0693891	0.8	0.6	9.3	764.7	2.6	0.0148234

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.475	17	2.2	0.0705521	4	0.449194	2.3	2.1	4.475	17.6	3.5	0.0715757
2	5.636	197	25.4	0.00889737	3	0.048307	0.2	0.2	5.636	196.9	3	0.00905979
3	5.792	225	29	0.0338149	4	0.254001	1.3	1.2	5.792	225.7	4.4	0.0323659
4	5.847	235	30.3	0.0186961	4	0.12858	0.7	0.6	5.847	234.8	4	0.0180191
5	5.923	250	32.2	0.396593	4	2.5322	13	11.7	5.923	250.4	3.5	0.405038
6	5.999	265	34.1	0.199452	5	1.71183	8.8	7.9	5.999	265.2	5.3	0.181395
7	6.094	284	36.6	0.130803	5	1.13996	5.9	5.3	6.094	283.7	5.2	0.123394
8	6.145	294	37.9	0.1044	4	0.717019	3.7	3.3	6.145	294.7	3.9	0.104072
9	6.22	309	39.8	0.0180264	3	0.0973266	0.5	0.5	6.22	308.8	3	0.0182047
10	6.316	328	42.3	0.0888272	4	0.664053	3.4	3.1	6.316	328.4	4.4	0.085873
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.422	349	45	0.0307547	3	0.152119	0.8	0.7	6.422	348.8	2.9	0.0298905
12	6.462	357	46	0.0191755	2	0.0715776	0.4	0.3	6.462	357.5	2	0.0199941
13	6.532	371	47.8	0.0100332	6	0.103555	0.5	0.5	6.532	368.9	6.5	0.00905683
14	6.638	385	49.6	0.0940143	3	0.477096	2.5	2.2	6.638	384	3	0.09039
15	6.688	391	50.4	0.119427	4	0.784462	4	3.6	6.688	390.9	3.7	0.118322
16	6.754	399	51.4	0.299278	3	1.39133	7.2	6.4	6.754	398.7	2.6	0.302439
17	6.862	412	53.1	0.390924	3	2.11032	10.8	9.8	6.862	412.1	2.9	0.40362
18	6.98	427	55	0.302947	3	1.80793	9.3	8.4	6.98	427.1	3.4	0.299076
19	7.185	453	58.4	0.0937714	4	0.640055	3.3	3	7.185	452.9	4	0.0906549
20	7.827	556	71.6	0.202871	13	4.17317	21.5	19.3	7.827	558.1	13.1	0.1804

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	29	3.7	0.0694189	4	0.524315	6.4	4.7	4.55	28.7	4.4	0.0668055
2	5.2	118	15.2	0.074548	6	0.733512	9	6.6	5.2	116.9	6.1	0.0681736
3	5.85	235	30.3	0.0878668	5	0.698031	8.6	6.2	5.85	234.1	4.6	0.085104
4	6.55	370	47.7	0.122813	8	1.59708	19.6	14.3	6.55	368.8	7.9	0.114333
5	6.85	407	52.5	0.0374336	5	0.294951	3.6	2.6	6.85	407.4	5	0.0335674
6	7.35	471	60.8	0.0795103	5	0.601295	7.4	5.4	7.35	471	4.6	0.0738303
7	8.15	608	78.5	0.0965158	8	1.32018	16.2	11.8	8.15	607.1	8.2	0.0905831
8	8.45	671	86.6	0.127377	7	1.48428	18.2	13.3	8.45	670.6	6.8	0.12282
9	8.65	717	92.5	0.11427	4	0.828083	10.1	7.4	8.65	716.3	4.3	0.108129
10	9.3	761	98.2	0.0158319	3	0.0783721	1	0.7	9.3	760.4	2.7	0.016272

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.437	13	1.7	0.0321456	4	0.20451	1.4	1.3	4.437	13.5	3.7	0.0312074
2	4.55	28	3.6	0.043865	3	0.265553	1.8	1.7	4.55	28.1	3.4	0.0443946
3	5.2	114	14.7	0.046351	5	0.406948	2.8	2.6	5.2	114.9	4.9	0.0464257
4	5.85	235	30.3	0.0262628	6	0.248008	1.7	1.6	5.85	233.5	5.6	0.0251824
5	5.935	251	32.4	0.266534	4	1.73217	11.8	11	5.935	250.5	3.7	0.265606
6	6.014	266	34.3	0.135082	5	1.19165	8.1	7.6	6.014	265.5	5.4	0.124458
7	6.115	285	36.8	0.0855122	5	0.725155	4.9	4.6	6.115	284.6	5.3	0.0773294
8	6.173	296	38.2	0.0682471	3	0.436329	3	2.8	6.173	295.8	3.5	0.0703748
9	6.242	309	39.9	0.0122153	3	0.0634011	0.4	0.4	6.242	308.9	2.9	0.0124834
10	6.343	328	42.3	0.0504463	4	0.314213	2.1	2	6.343	327.1	3.5	0.0504989
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.444	347	44.8	0.0235388	2	0.106183	0.7	0.7	6.444	346.2	2.4	0.0254406
12	6.476	353	45.5	0.00943752	1	0.0280074	0.2	0.2	6.476	353.6	1.5	0.0107865
13	6.55	367	47.4	0.0239422	5	0.212518	1.4	1.3	6.55	366.5	5.1	0.0233706
14	6.64	380	49	0.049203	2	0.17859	1.2	1.1	6.64	379.6	2.4	0.0421937
15	6.682	386	49.8	0.0836803	5	0.640753	4.4	4.1	6.682	387	4.5	0.0795841
16	6.737	394	50.8	0.241887	2	0.884028	6	5.6	6.737	393.9	2.2	0.231963
17	6.827	407	52.5	0.364665	3	1.62328	11	10.3	6.827	406.7	2.5	0.365661
18	6.956	423	54.6	0.222725	3	1.11463	7.6	7.1	6.956	422.5	2.8	0.226999
19	7.182	450	58.1	0.0622925	3	0.320259	2.2	2	7.182	449.7	3	0.0598172
20	7.35	470	60.6	0.0263933	5	0.199014	1.4	1.3	7.35	469.1	4.7	0.0239034
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.858	557	71.9	0.128309	12	2.14609	14.6	13.6	7.858	557.3	11.9	0.10213
22	8.15	607	78.3	0.0355883	11	0.620594	4.2	3.9	8.15	606.8	10.8	0.0325524
23	8.45	674	87	0.0539151	5	0.468264	3.2	3	8.45	674.5	4.9	0.0544559
24	8.65	718	92.6	0.0516434	4	0.343859	2.3	2.2	8.65	717.7	3.9	0.0495775
25	9.3	761	98.2	0.018077	8	0.218008	1.5	1.4	9.3	760	8.3	0.0148591

PeakCounter: 25

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.493	17	2.2	0.0556312	5	0.440857	2.4	2	4.493	17.4	4.5	0.0549174
2	5.651	199	25.6	0.0248591	12	0.487612	2.7	2.2	5.651	200.8	11.6	0.0236693
3	5.842	234	30.1	0.0116273	6	0.111551	0.6	0.5	5.842	232.1	5.6	0.0113163
4	5.933	252	32.4	0.295522	4	2.26975	12.5	10.2	5.933	252.2	4.2	0.304438
5	5.999	265	34.1	0.180003	4	1.3542	7.5	6.1	5.999	265.1	4.4	0.174861
6	6.1	285	36.7	0.13972	6	1.36223	7.5	6.1	6.1	284.9	5.9	0.130563
7	6.161	297	38.2	0.106675	4	0.687513	3.8	3.1	6.161	297.4	3.7	0.105813
8	6.237	312	40.2	0.0178458	3	0.0966532	0.5	0.4	6.237	311.7	3	0.0179958
9	6.328	330	42.5	0.0979214	4	0.610712	3.4	2.7	6.328	329.7	3.5	0.0972763
10	6.424	349	44.9	0.0266717	3	0.135031	0.7	0.6	6.424	348.4	3.1	0.0246713
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.459	356	45.8	0.0166397	2	0.0510071	0.3	0.2	6.459	356.1	1.7	0.0168836
12	6.494	363	46.7	0.0118223	4	0.0812174	0.4	0.4	6.494	364.5	4.4	0.0103934
13	6.603	381	49	0.103889	3	0.475924	2.6	2.1	6.603	380.2	3.1	0.0879661
14	6.641	386	49.7	0.131578	4	0.938589	5.2	4.2	6.641	386.9	4.1	0.127621
15	6.701	394	50.7	0.319709	2	1.26986	7	5.7	6.701	393.9	2.3	0.312213
16	6.8	407	52.4	0.411493	3	1.92551	10.6	8.6	6.8	406.9	2.6	0.410842
17	6.932	424	54.6	0.305925	3	1.65911	9.2	7.4	6.932	423.8	3.2	0.296166
18	7.155	452	58.2	0.0939288	3	0.486604	2.7	2.2	7.155	451.8	3	0.0909207
19	7.822	555	71.4	0.177144	13	3.66883	20.3	16.4	7.822	557.4	13.3	0.15553

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	20	2.6	0.07436	4	0.463448	5.6	4.3	4.55	20.7	3.7	0.0713777
2	5.2	116	14.9	0.0688854	6	0.712396	8.6	6.6	5.2	116.9	6.1	0.0660197
3	5.85	234	30.2	0.0819984	5	0.685171	8.3	6.4	5.85	233.2	5	0.0778675
4	6.55	379	48.8	0.132721	6	1.23967	15	11.6	6.55	378	5.7	0.123042
5	6.85	415	53.5	0.0227871	6	0.236397	2.9	2.2	6.85	415.9	6.5	0.0206241
6	7.35	481	62	0.0466101	5	0.402842	4.9	3.8	7.35	480.8	5.2	0.0438038
7	8.15	610	78.6	0.096229	10	1.72995	20.9	16.1	8.15	609.6	10.4	0.0940351
8	8.45	683	88	0.107986	8	1.54066	18.6	14.4	8.45	682.1	8.3	0.105196
9	8.65	723	93.2	0.0944825	5	0.802091	9.7	7.5	8.65	723.6	5.2	0.0877439
10	9.3	758	97.7	0.0252903	11	0.460286	5.6	4.3	9.3	754.9	11.2	0.0232068

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 94

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 5.700 5.200 8.100 5.850 8.600 6.550 16.500 6.850 6.400 7.350 6.500 8.150 16.700 8.450 20.000 8.650 10.600 9.300 0.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.475 2.300 5.636 0.200 5.792 1.300 5.847 0.700 5.923 13.000 5.999 8.800 6.094 5.900 6.145 3.700 6.220 0.500 6.316 3.400 6.422 0.800 6.462 0.400 6.532 0.500 6.638 2.500 6.688 4.000 6.754 7.200 6.862 10.800 6.980 9.300 7.185 3.300 7.827 21.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 20 Return to Lane Navigator
4.550 6.400 5.200 9.000 5.850 8.600 6.550 19.600 6.850 3.600 7.350 7.400 8.150 16.200 8.450 18.200 8.650 10.100 9.300 1.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.437 1.400 4.550 1.800 5.200 2.800 5.850 1.700 5.935 11.800 6.014 8.100 6.115 4.900 6.173 3.000 6.242 0.400 6.343 2.100 6.444 0.700 6.476 0.200 6.550 1.400 6.640 1.200 6.682 4.400 6.737 6.000 6.827 11.000 6.956 7.600 7.182 2.200 7.350 1.400 7.858 14.600 8.150 4.200 8.450 3.200 8.650 2.300 9.300 1.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 25 Return to Lane Navigator
4.493 2.400 5.651 2.700 5.842 0.600 5.933 12.500 5.999 7.500 6.100 7.500 6.161 3.800 6.237 0.500 6.328 3.400 6.424 0.700 6.459 0.300 6.494 0.400 6.603 2.600 6.641 5.200 6.701 7.000 6.800 10.600 6.932 9.200 7.155 2.700 7.822 20.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 19 Return to Lane Navigator
4.550 5.600 5.200 8.600 5.850 8.300 6.550 15.000 6.850 2.900 7.350 4.900 8.150 20.900 8.450 18.600 8.650 9.700 9.300 5.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

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Fred Fry E-mail: frf@vm.cfsan.fda.gov

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Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Oilfish(*) ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Oilfish(*) ---- Go to Display Gel Lane Navigator.