Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Dolphin  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Coryphaena hippurus
FDA Seafood List Common Name: Dolphin
FDA Seafood List Market Name: Mahi Mahi
Unique IEF Gel Code: 19981214gel03
Unique Web Code: mhh01Srr
Tissue Aliquot Code: coryhipp J98-001 #3 Gel Analysis Conditions
Gel ImageFillet ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.554050.11331130.618073.22.54.55403.20.109284
25.212916.30.098491850.8559654.43.55.2128.85.10.0952691
35.8524030.30.18070441.238546.45.15.85239.43.80.184596
46.5537847.70.24511162.2756911.89.36.553775.60.227813
56.85412520.33285642.11709118.66.85411.73.70.32208
67.3548160.71.6497226.2862832.625.67.35480.92.11.65411
78.1560075.70.0685708101.251996.55.18.15599.19.60.0737687
88.4566083.20.23337572.6328313.710.78.45659.86.60.224634
98.6571389.90.18502941.258926.55.18.6571340.177705
109.376696.60.0514617100.7343523.839.3763.310.10.0409862
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
15.33614418.20.044740630.2459750.20.25.336143.83.50.0401333
25.63819624.70.031084540.2091860.20.25.638195.73.90.0304827
35.80622528.40.17042841.041520.90.85.806225.43.60.162261
45.9825932.70.048461640.279150.20.25.98258.33.60.0441833
56.23631139.30.054543190.6953410.60.56.236311.78.90.0439433
66.344333422.31491313.747711.610.76.344334.72.92.65724
76.43235144.32.2557210.50258.98.26.432349.92.32.55154
86.47135945.30.35961631.580481.31.26.471359.52.60.33719
96.50136546.12.1649315.183114.446.501365.11.42.14898
106.55937647.50.083660520.2669160.20.26.559376.61.50.0975675
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.65638748.90.50421521.799351.51.46.6563872.40.417954
126.75339850.32.0969414.6811143.66.753397.91.32.06197
136.85741051.82.2964629.773758.37.66.857409.22.12.61664
146.92441952.90.30583531.528091.31.26.924418.62.90.301405
157.07243955.40.024431430.1132430.10.17.072439.42.70.0237007
167.16145156.90.050335210007.16144410
177.22459582.2831627.951936.76.27.224591.72.68593
187.38748260.92.30128416.862114.213.17.387480.53.72.56227
198.00857472.52.35574418.507515.614.48.0085723.82.73926
208.06958373.60.5616843.978943.43.18.069583.54.10.554226
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.39464581.40.02549230.1378040.10.18.394645.92.80.0277863
228.45965983.20.045044740.3040740.30.28.459658.23.70.0460589
238.56568786.70.249167188.2545976.48.565697.6180.258735
248.71771590.31.0152569.799038.37.68.717714.26.20.889804
258.98473793.10.044049930.1112630.10.18.984738.930.021251
269.21575695.50.12546340.7821710.70.69.215755.63.60.121987
PeakCounter: 26
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55303.80.098292630.5754123.62.74.5530.13.40.0944349
25.211314.20.1123240.7821334.93.75.2113.14.30.103537
35.8522628.50.23104831.368878.76.55.85225.63.40.228947
46.55373470.20756361.9663812.49.36.55371.860.186194
56.8540751.30.34833831.8275711.68.66.85407.53.20.324404
67.3547359.61.285623.5705922.616.97.35472.71.91.08428
78.1559174.50.10103991.517579.67.28.15591.69.10.0939858
88.4565482.50.22535162.3378814.8118.45653.360.219665
98.6570789.20.18927541.219157.75.88.65706.63.90.177046
109.3761960.072262560.6457784.139.3760.85.60.0651839
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55415.20.042511740.2917120.40.44.5540.63.80.0429653
25.212816.10.051586360.5586120.80.75.2127.96.30.0498751
35.7521627.20.087624930.5008830.70.75.752163.30.085221
45.8523229.30.085975140.5526470.80.75.85231.73.60.0866494
56.22730738.70.0296322100.4902580.70.66.227308.910.50.0263536
66.34733141.72.33878211.72616.715.46.347332.62.52.68973
76.438349441.3774324.2218665.56.438349.21.81.3003
86.49336045.40.1855430.9979081.41.36.493359.93.30.17178
96.518365460.32903931.351161.91.86.518365.92.50.302984
106.68438748.80.055759530.2783830.40.46.684387.63.10.0511282
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.78739950.30.20372441.272831.81.76.787400.63.70.193324
126.88341151.82.2963229.624813.712.66.883410.12.12.61455
137.20345757.60.32656431.448562.11.97.203457.92.50.322559
147.3547860.32.32508415.831522.520.87.35477.63.72.39711
158.00557071.92.34315210.57521513.98.005569.62.22.68728
168.07658073.10.41344442.554433.63.48.076580.44.40.330603
178.4565582.60.074264160.7914211.118.45654.75.90.0760234
188.54267985.60.0829761181.990942.82.68.542687180.0624049
198.6570789.20.36078951.946162.82.68.65707.54.60.236767
208.98973292.30.0774198182.673743.83.58.989718.7180.083807
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
219.375595.20.099096960.72108110.99.3753.95.60.0722962
PeakCounter: 21
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
15.23113316.80.028874140.2092080.20.25.231132.74.30.0271492
25.54218323.10.029064940.201640.20.25.5421823.90.0291757
35.71621126.60.14254730.6955390.60.65.716210.82.80.13839
45.919246310.052930630.2735970.20.25.919246.13.10.0490901
56.2130338.20.05752750.4766390.40.46.21303.15.30.0510575
66.31732440.92.30355313.44412.110.86.317325.32.92.642
76.404341432.2569210.37769.48.36.404340.82.42.47092
86.45535144.30.34996731.672131.51.36.455351.12.90.320967
96.485357451.744314.3832143.56.485356.91.51.65193
106.54736946.50.10084120.3163810.30.36.547369.41.70.105321
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.644381480.45348842.350122.11.96.644381.43.60.367963
126.73639249.42.2950615.448824.94.46.736392.31.22.47873
136.83640450.92.3034528.664777.86.96.836403.81.82.65287
146.91141452.20.34465631.618451.51.36.911413.52.70.333313
157.0543354.60.041561540.2323510.20.27.05433.83.50.0371611
167.19645357.12.3036126.943786.35.67.196453.31.52.57698
177.32847159.42.28992314.80713.411.97.328473.33.12.66958
187.98656671.42.33813416.734415.113.47.986564.93.62.59117
198.06257772.80.7467844.959274.548.062577.840.696266
208.44165382.30.055684330.3080710.30.28.441652.33.10.0557325
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.54868185.90.232054187.666236.96.18.548703.6180.240294
228.6570889.31.1254757.292446.65.88.65708.64.70.873754
238.97273392.40.14510430.4799470.40.48.972733.53.40.0786611
249.24275495.10.23218941.336781.21.19.242752.93.80.200265
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55374.70.11198430.6025624.73.54.5536.730.112256
25.213016.40.12786640.8709316.85.15.2129.640.122975
35.8523529.60.24721231.244999.77.35.85234.72.90.240666
46.5537046.60.14039291.9056714.911.16.55367.88.50.126192
56.8540751.30.23187131.217119.57.16.854073.10.219668
67.3547259.40.51055732.5105419.614.77.35472.22.80.503119
78.1559174.40.12311161.2988410.27.68.15590.46.50.113572
88.4565782.70.14969561.6311112.89.58.45656.86.30.146465
98.6571189.50.13807140.8501226.658.65710.43.70.130745
109.376496.20.073172950.6589025.23.99.3763.65.50.0677377
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 8.65 label for gel schematic
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pI 9.30 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 101
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 26 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 21 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln