RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Lane Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lutjanus synagris
FDA Seafood List Common Name: Lane Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19940817gel02
Unique Web Code: lsh02Srr
Tissue Aliquot Code: lutjsyna 001 #5 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	105	12.4	0.0405855	7	0.452926	9.3	7.5	4.55	105.2	6.9	0.0371235
2	5.2	203	24	0.0360554	5	0.295231	6.1	4.9	5.2	203.2	4.9	0.0337378
3	5.85	323	38.2	0.0849837	4	0.637421	13.1	10.5	5.85	323.2	4.3	0.084573
4	6.55	450	53.2	0.036457	10	0.598867	12.3	9.9	6.55	448	10	0.0338203
5	7.35	543	64.2	0.0195474	6	0.203112	4.2	3.3	7.35	543	6.1	0.0186671
6	8.15	660	78	0.053307	7	0.613771	12.6	10.1	8.15	660.3	6.8	0.0511967
7	8.45	723	85.5	0.0611684	7	0.785848	16.1	13	8.45	723.1	7.4	0.0598714
8	8.65	769	90.9	0.0536689	5	0.452649	9.3	7.5	8.65	768.7	4.9	0.051878
9	9.3	819	96.8	0.0711697	7	0.839788	17.2	13.8	9.3	818.9	7	0.0678076

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.073	34	4	0.0670608	8	0.914065	3.5	3.1	4.073	34.5	7.7	0.0669608
2	4.804	142	16.8	0.240798	7	2.89116	11.1	9.7	4.804	143.2	7.1	0.230696
3	4.953	164	19.4	0.169459	9	2.56425	9.9	8.6	4.953	162.3	8.8	0.163918
4	5.318	222	26.2	0.016884	4	0.119228	0.5	0.4	5.318	220.6	4.3	0.0155188
5	5.401	237	28	0.417709	3	2.21941	8.5	7.4	5.401	236.8	3.3	0.378776
6	5.506	256	30.3	0.0472493	17	1.53608	5.9	5.1	5.506	245	17	0.0509655
7	5.643	281	33.2	0.0425903	4	0.280141	1.1	0.9	5.643	281.4	4.1	0.038114
8	5.928	333	39.4	0.0189511	5	0.165031	0.6	0.6	5.928	334.5	4.9	0.0188487
9	6.046	355	42	0.534509	3	3.10273	11.9	10.4	6.046	354.9	3.3	0.524433
10	6.132	371	43.9	0.262491	4	1.74724	6.7	5.8	6.132	370.9	4	0.248337
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.228	389	46	0.137314	6	1.52624	5.9	5.1	6.228	387.2	6	0.144553
12	6.496	439	51.9	0.0567924	8	0.84459	3.3	2.8	6.496	441.8	8.4	0.056523
13	6.714	468	55.3	0.362047	3	2.03955	7.9	6.8	6.714	468.2	3.5	0.332885
14	6.887	488	57.7	0.0355863	8	0.462886	1.8	1.5	6.887	489.5	7.9	0.0331747
15	7	501	59.2	0.209917	4	1.37711	5.3	4.6	7	501.1	4	0.195641
16	7.129	516	61	0.414084	4	2.85317	11	9.6	7.129	516.1	3.9	0.412721
17	7.276	533	63	0.0759968	3	0.420804	1.6	1.4	7.276	533.2	3	0.0781737
18	7.448	556	65.7	0.0530191	9	0.912948	3.5	3.1	7.448	554.7	9.4	0.0545697

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	108	12.8	0.0552624	7	0.629959	9.9	7.9	4.55	108.2	6.7	0.0527783
2	5.2	202	23.9	0.0703863	4	0.502848	7.9	6.3	5.2	201.2	4.2	0.0681609
3	5.85	318	37.6	0.119397	4	0.923573	14.6	11.6	5.85	317.9	4.4	0.118976
4	6.55	452	53.4	0.058476	8	0.777906	12.3	9.7	6.55	450.6	7.7	0.0567618
5	7.35	544	64.3	0.026153	6	0.26821	4.2	3.4	7.35	543.9	6.2	0.0244592
6	8.15	663	78.4	0.0617076	6	0.668194	10.5	8.4	8.15	662.2	6.4	0.0584531
7	8.45	720	85.1	0.0640588	7	0.776309	12.2	9.7	8.45	720.4	7	0.0627497
8	8.65	768	90.8	0.0554606	6	0.537908	8.5	6.7	8.65	767.4	5.7	0.0529127
9	9.3	820	96.9	0.115713	6	1.2525	19.8	15.7	9.3	819.8	6.4	0.111157

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.087	45	5.3	0.0191955	7	0.247067	1.6	1.5	4.087	45.9	7.4	0.0188279
2	4.55	112	13.2	0.0224421	5	0.185806	1.2	1.1	4.55	111.6	5	0.0208605
3	4.778	145	17.1	0.133655	6	1.42234	9.3	8.4	4.778	145.2	6.5	0.124134
4	4.903	163	19.3	0.0647431	11	1.30815	8.5	7.7	4.903	161.1	11	0.067266
5	5.2	206	24.3	0.0192042	4	0.141802	0.9	0.8	5.2	206	4.2	0.0189146
6	5.285	221	26.1	0.0100201	3	0.0490858	0.3	0.3	5.285	220.7	2.8	0.0097515
7	5.37	236	27.9	0.24471	3	1.39834	9.1	8.2	5.37	236	3.5	0.228371
8	5.471	254	30	0.0247206	13	0.586763	3.8	3.5	5.471	247.3	13.1	0.0253324
9	5.601	277	32.7	0.0195597	6	0.192794	1.3	1.1	5.601	275.6	5.9	0.0183362
10	5.85	321	37.9	0.0533605	4	0.374467	2.4	2.2	5.85	321.1	4	0.0531957
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.104	368	43.5	0.276411	3	1.62433	10.6	9.6	6.104	367.9	3.4	0.265884
12	6.169	380	44.9	0.150409	4	1.12336	7.3	6.6	6.169	379.5	4.4	0.143767
13	6.233	392	46.3	0.10301	5	0.87134	5.7	5.1	6.233	392	4.7	0.10395
14	6.492	440	52	0.0410764	8	0.567502	3.7	3.3	6.492	440.7	8	0.0401428
15	6.68	466	55.1	0.178784	3	0.745178	4.9	4.4	6.68	465.7	2.7	0.155618
16	6.833	484	57.2	0.0248934	18	0.710396	4.6	4.2	6.833	474.8	18	0.022267
17	6.943	497	58.7	0.0710477	3	0.323254	2.1	1.9	6.943	497.4	2.8	0.0641271
18	7.087	514	60.8	0.263593	3	1.58001	10.3	9.3	7.087	513.7	3.4	0.263223
19	7.257	534	63.1	0.0343266	3	0.195212	1.3	1.1	7.257	534.3	3.3	0.0335188
20	7.35	545	64.4	0.0092179	4	0.0609975	0.4	0.4	7.35	544.1	4	0.0086647
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.482	564	66.7	0.0221673	7	0.267521	1.7	1.6	7.482	563.3	7.2	0.0208684
22	8.15	660	78	0.0235168	6	0.206215	1.3	1.2	8.15	660.7	5.9	0.0198458
23	8.45	725	85.7	0.0318207	7	0.372661	2.4	2.2	8.45	723.7	7	0.030218
24	8.65	769	90.9	0.0255287	5	0.225053	1.5	1.3	8.65	768.1	5.1	0.0246814
25	9.3	817	96.6	0.0425352	7	0.52385	3.4	3.1	9.3	817.1	7.4	0.0397204

PeakCounter: 25

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.021	31	3.7	0.0892438	8	1.17163	4.4	3.9	4.021	31.6	7.7	0.0860321
2	4.758	141	16.6	0.265086	6	2.77625	10.5	9.2	4.758	140.9	6.2	0.250754
3	4.898	162	19.1	0.121678	11	2.35111	8.9	7.8	4.898	159.7	11.1	0.119938
4	5.29	223	26.3	0.0099817	4	0.0718471	0.3	0.2	5.29	222.7	4.2	0.0096304
5	5.379	239	28.2	0.415468	4	2.69329	10.2	8.9	5.379	238.7	3.9	0.390958
6	5.469	255	30.1	0.0734699	13	1.52564	5.8	5	5.469	251.8	12.7	0.0675948
7	5.587	276	32.5	0.065795	6	0.614072	2.3	2	5.587	276.3	5.8	0.0598474
8	5.986	348	41	0.0212616	3	0.127962	0.5	0.4	5.986	347.6	3.4	0.0215088
9	6.063	362	42.7	0.446317	3	2.72744	10.4	9	6.063	361.9	3.5	0.44016
10	6.139	376	44.3	0.280067	4	1.91522	7.3	6.3	6.139	375.4	4	0.270985
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.21	389	45.9	0.175373	5	1.56337	5.9	5.2	6.21	388.3	4.8	0.183268
12	6.275	401	47.3	0.0060515	1	0.004832	0	0	6.275	402	1.4	0.0019998
13	6.466	436	51.4	0.0595059	6	0.671394	2.5	2.2	6.466	436.3	6.4	0.0589331
14	6.599	457	53.9	0.0842163	3	0.338596	1.3	1.1	6.599	456.9	2.7	0.0708809
15	6.652	463	54.6	0.402161	2	1.6089	6.1	5.3	6.652	463	2.4	0.375171
16	6.835	484	57.1	0.0423218	20	1.33242	5.1	4.4	6.835	478	19.5	0.0385379
17	6.949	497	58.6	0.197815	3	1.05488	4	3.5	6.949	496.7	3.3	0.179667
18	7.097	514	60.6	0.399606	4	2.51463	9.5	8.3	7.097	514.5	3.6	0.399049
19	7.271	534	63	0.0797307	3	0.432199	1.6	1.4	7.271	533.5	3	0.0804854
20	7.459	559	65.9	0.0441017	11	0.84756	3.2	2.8	7.459	556.5	10.8	0.0442254

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	106	12.5	0.0659392	7	0.776319	9.1	7.6	4.55	106.5	7.2	0.0604681
2	5.2	206	24.3	0.0913815	4	0.653058	7.7	6.4	5.2	205.7	4.2	0.088341
3	5.85	323	38.2	0.156959	4	1.18696	14	11.6	5.85	322.8	4.3	0.154434
4	6.55	450	53.2	0.0638595	8	0.923877	10.9	9	6.55	448.5	8.1	0.064229
5	7.35	539	63.7	0.0446071	6	0.430948	5.1	4.2	7.35	539.2	5.5	0.0439266
6	8.15	659	77.9	0.0732513	7	0.89954	10.6	8.8	8.15	659.4	7.2	0.0700352
7	8.45	719	85	0.0790386	9	1.28211	15.1	12.5	8.45	717.9	9.4	0.0769278
8	8.65	761	90	0.0830607	5	0.73926	8.7	7.2	8.65	760.4	5.2	0.0807518
9	9.3	814	96.2	0.138214	7	1.59737	18.8	15.6	9.3	813.4	6.8	0.131591

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 90

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 9.300 5.200 6.100 5.850 13.100 6.550 12.300 7.350 4.200 8.150 12.600 8.450 16.100 8.650 9.300 9.300 17.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.073 3.500 4.804 11.100 4.953 9.900 5.318 0.500 5.401 8.500 5.506 5.900 5.643 1.100 5.928 0.600 6.046 11.900 6.132 6.700 6.228 5.900 6.496 3.300 6.714 7.900 6.887 1.800 7.000 5.300 7.129 11.000 7.276 1.600 7.448 3.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 18 Return to Lane Navigator
4.550 9.900 5.200 7.900 5.850 14.600 6.550 12.300 7.350 4.200 8.150 10.500 8.450 12.200 8.650 8.500 9.300 19.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.087 1.600 4.550 1.200 4.778 9.300 4.903 8.500 5.200 0.900 5.285 0.300 5.370 9.100 5.471 3.800 5.601 1.300 5.850 2.400 6.104 10.600 6.169 7.300 6.233 5.700 6.492 3.700 6.680 4.900 6.833 4.600 6.943 2.100 7.087 10.300 7.257 1.300 7.350 0.400 7.482 1.700 8.150 1.300 8.450 2.400 8.650 1.500 9.300 3.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 25 Return to Lane Navigator
4.021 4.400 4.758 10.500 4.898 8.900 5.290 0.300 5.379 10.200 5.469 5.800 5.587 2.300 5.986 0.500 6.063 10.400 6.139 7.300 6.210 5.900 6.275 0 6.466 2.500 6.599 1.300 6.652 6.100 6.835 5.100 6.949 4.000 7.097 9.500 7.271 1.600 7.459 3.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 20 Return to Lane Navigator
4.550 9.100 5.200 7.700 5.850 14.000 6.550 10.900 7.350 5.100 8.150 10.600 8.450 15.100 8.650 8.700 9.300 18.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Lane Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Lane Snapper ---- Go to Display Gel Lane Navigator.