RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Lane Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lutjanus synagris
FDA Seafood List Common Name: Lane Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19940817gel01
Unique Web Code: lsg02Srr
Tissue Aliquot Code: lutjsyna 001 #5 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	36	3.2	0.264244	10	4.35595	14.5	11.1	3.5	37.8	9.6	0.255315
2	4.55	188	16.8	0.26127	7	2.77144	9.2	7	4.55	188.2	6.5	0.239782
3	5.2	270	24.1	0.359507	6	3.47649	11.6	8.8	5.2	269.9	5.6	0.347529
4	5.85	416	37.2	0.408492	4	3.10557	10.3	7.9	5.85	415.8	4.3	0.408276
5	6.55	576	51.5	0.263367	6	2.68413	8.9	6.8	6.55	575.5	5.9	0.25455
6	6.85	615	55	0.102311	11	1.7998	6	4.6	6.85	617.1	10.7	0.0949615
7	7.35	707	63.2	0.265258	6	2.81729	9.4	7.2	7.35	706	6.1	0.259528
8	8.15	866	77.4	0.0894456	11	1.62898	5.4	4.1	8.15	866.1	10.6	0.0867341
9	8.45	930	83.1	0.137435	8	1.81912	6.1	4.6	8.45	929.5	7.8	0.131083
10	8.65	973	87	0.126204	7	1.49316	5	3.8	8.65	972.2	7	0.120008
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1103	98.6	0.326936	8	4.07965	13.6	10.4	9.3	1100.9	7.8	0.294902

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.791	77	6.9	0.0681144	8	0.919163	2.8	2.2	3.791	75.9	7.9	0.0655405
2	3.878	89	7.9	0.346863	6	3.4265	10.3	8.1	3.878	89.4	5.5	0.351276
3	4.611	190	17	0.0383001	6	0.386807	1.2	0.9	4.611	188.5	5.8	0.037777
4	4.691	200	17.9	0.0469076	5	0.431636	1.3	1	4.691	199	5.3	0.0461831
5	4.845	219	19.6	0.0152103	4	0.0932901	0.3	0.2	4.845	219.3	3.5	0.014916
6	4.966	234	20.9	0.0237168	4	0.121235	0.4	0.3	4.966	233.2	3.7	0.0184615
7	5.095	250	22.3	0.388822	5	3.64014	11	8.6	5.095	250.2	5.1	0.401111
8	5.252	275	24.6	0.0803685	4	0.510977	1.5	1.2	5.252	274.7	3.7	0.0770151
9	5.295	285	25.4	0.0993434	3	0.501822	1.5	1.2	5.295	284.3	2.7	0.106486
10	5.339	295	26.3	0.482622	4	3.02549	9.1	7.2	5.339	295	3.6	0.471614
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.482	328	29.3	0.05813	4	0.424701	1.3	1	5.482	329.1	4.2	0.0567889
12	5.581	351	31.3	0.0804675	4	0.566382	1.7	1.3	5.581	350.2	4.1	0.0782591
13	5.685	375	33.5	0.0245763	3	0.145411	0.4	0.3	5.685	374.5	3.4	0.0243227
14	5.755	391	34.9	0.0594142	4	0.362719	1.1	0.9	5.755	391.2	3.6	0.0562793
15	5.841	411	36.7	0.168663	4	1.13415	3.4	2.7	5.841	411.2	4.2	0.153415
16	5.971	441	39.4	0.541837	4	3.53099	10.6	8.3	5.971	440.8	3.7	0.544355
17	6.054	460	41.1	0.412565	4	2.5538	7.7	6	6.054	459.7	3.7	0.390943
18	6.114	474	42.3	0.136222	4	0.757511	2.3	1.8	6.114	473.2	3.8	0.111368
19	6.149	482	43	0.168225	5	1.21204	3.7	2.9	6.149	480.9	5	0.136862
20	6.439	549	49	0.117735	8	1.46553	4.4	3.5	6.439	546.9	7.9	0.104667
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.478	558	49.8	0.068026	2	0.207003	0.6	0.5	6.478	558.9	1.9	0.0614286
22	6.554	575	51.3	0.112804	2	0.358447	1.1	0.8	6.554	575	1.9	0.109262
23	6.623	584	52.1	0.379762	2	1.58204	4.8	3.7	6.623	583.9	2.3	0.392235
24	6.731	598	53.4	0.0405695	4	0.275192	0.8	0.7	6.731	597.3	4.1	0.0379762
25	6.896	622	55.5	0.0373599	2	0.145738	0.4	0.3	6.896	622.2	2.1	0.0383001
26	7.005	642	57.3	0.394829	3	2.09409	6.3	5	7.005	642.1	3	0.389968
27	7.119	663	59.2	0.336895	4	2.53086	7.6	6	7.119	663.6	4.3	0.330467
28	7.255	688	61.4	0.115751	4	0.76797	2.3	1.8	7.255	688.2	3.8	0.113803

PeakCounter: 28

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	37	3.3	0.322816	13	6.87139	19.9	14.8	3.5	38.6	12.9	0.299935
2	4.55	177	15.8	0.204855	9	3.02594	8.8	6.5	4.55	177.8	8.5	0.200313
3	5.2	256	22.8	0.247456	9	3.70279	10.7	8	5.2	257.1	8.7	0.24151
4	5.85	410	36.6	0.29279	6	3.14264	9.1	6.8	5.85	410.2	5.9	0.301901
5	6.55	573	51.1	0.295809	6	2.90243	8.4	6.2	6.55	572.3	5.8	0.281689
6	6.85	612	54.6	0.112328	10	1.80192	5.2	3.9	6.85	612.8	10	0.101307
7	7.35	704	62.8	0.283487	6	2.83993	8.2	6.1	7.35	703.7	5.8	0.274581
8	8.15	864	77.1	0.0915942	11	1.78352	5.2	3.8	8.15	864.6	11.4	0.0883129
9	8.45	928	82.8	0.171614	8	2.26937	6.6	4.9	8.45	927.6	7.7	0.165654
10	8.65	970	86.5	0.162246	7	1.85217	5.4	4	8.65	968.9	6.7	0.156508
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1097	97.9	0.350755	8	4.35723	12.6	9.4	9.3	1095	7.8	0.316139

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	40	3.6	0.22265	10	3.82621	7.3	6.4	3.5	41.9	10	0.21683
2	3.768	77	6.9	0.0614767	6	0.617871	1.2	1	3.768	76.4	5.9	0.0588235
3	3.862	90	8.1	0.34613	5	3.34656	6.4	5.6	3.862	89.8	5.3	0.355078
4	4.42	167	15	0.0352779	6	0.387979	0.7	0.6	4.42	168.6	6.5	0.033689
5	4.55	185	16.6	0.229706	5	2.02055	3.9	3.4	4.55	185.2	5.2	0.217907
6	4.672	200	17.9	0.0813969	7	0.992174	1.9	1.7	4.672	197.4	6.8	0.082001
7	4.826	219	19.6	0.0194712	2	0.0675034	0.1	0.1	4.826	218.4	2	0.0185864
8	4.891	227	20.3	0.0291469	6	0.253532	0.5	0.4	4.891	229.1	6.2	0.0232129
9	5.062	248	22.2	0.369776	5	3.30738	6.3	5.5	5.062	248	4.8	0.387839
10	5.2	265	23.7	0.195142	5	1.47024	2.8	2.5	5.2	264.5	4.6	0.181613
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.257	278	24.9	0.0542923	8	0.887641	1.7	1.5	5.257	274.8	7.8	0.0638683
12	5.314	291	26.1	0.512536	3	2.6754	5.1	4.5	5.314	291.7	3	0.505583
13	5.464	325	29.1	0.0745273	3	0.450165	0.9	0.8	5.464	325.3	3.4	0.0738389
14	5.56	347	31.1	0.0562332	5	0.4546	0.9	0.8	5.56	347	5	0.0513157
15	5.696	378	33.9	0.0646259	13	1.1629	2.2	1.9	5.696	377.8	13.3	0.0492847
16	5.74	388	34.8	0.0325145	1	0.0160518	0	0	5.74	389	1.3	0.00717543
17	5.793	400	35.8	0.0467856	3	0.264456	0.5	0.4	5.793	399.2	3.3	0.0452021
18	5.85	413	37	0.276983	4	1.87631	3.6	3.1	5.85	413.6	3.7	0.28324
19	5.981	441	39.5	0.508171	4	4.02036	7.7	6.7	5.981	441.3	4.3	0.523389
20	6.083	463	41.5	0.340712	4	2.47365	4.7	4.1	6.083	462.1	4.3	0.328
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.163	480	43	0.120634	7	1.5345	2.9	2.6	6.163	479	6.7	0.129836
22	6.256	500	44.8	0.0109115	2	0.0354116	0.1	0.1	6.256	500.9	2.1	0.00942416
23	6.499	552	49.5	0.113592	9	1.75661	3.4	2.9	6.499	550.6	9	0.110151
24	6.55	563	50.4	0.147392	5	1.03435	2	1.7	6.55	563.4	4.8	0.122648
25	6.67	579	51.9	0.106724	2	0.350448	0.7	0.6	6.67	579.1	1.9	0.106577
26	6.738	588	52.7	0.386602	2	1.38927	2.7	2.3	6.738	588.2	2	0.38717
27	6.85	603	54	0.0579313	7	0.684082	1.3	1.1	6.85	605.5	7	0.055288
28	6.923	617	55.3	0.0285742	4	0.189832	0.4	0.3	6.923	617.9	4.5	0.0238054
29	6.975	627	56.2	0.0166832	2	0.0436112	0.1	0.1	6.975	627.5	2	0.0122155
30	7.069	645	57.8	0.359135	3	1.91181	3.7	3.2	7.069	645.7	3	0.362622
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.209	672	60.2	0.320254	5	2.69724	5.2	4.5	7.209	671.5	4.8	0.31519
32	7.35	699	62.6	0.244274	6	2.43883	4.7	4.1	7.35	698.5	5.6	0.245006
33	8.15	861	77.2	0.0324316	18	1.43359	2.7	2.4	8.15	871.9	18	0.0449351
34	8.45	920	82.4	0.0946654	8	1.21203	2.3	2	8.45	920.7	7.7	0.089167
35	8.65	965	86.5	0.11915	7	1.39454	2.7	2.3	8.65	964.7	6.8	0.115719
36	9.3	1096	98.2	0.298633	7	3.53377	6.8	5.9	9.3	1094.2	7.4	0.268459

PeakCounter: 36

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.771	80	7.1	0.074371	7	0.826506	2.6	2.1	3.771	79.2	6.6	0.070526
2	3.859	92	8.1	0.344373	5	3.30133	10.5	8.6	3.859	91.8	5.4	0.346605
3	4.579	190	16.8	0.0407442	6	0.390396	1.2	1	4.579	190.5	5.8	0.0382826
4	4.678	202	17.9	0.047838	5	0.411557	1.3	1.1	4.678	201	4.9	0.0472821
5	4.842	222	19.7	0.0159945	3	0.0790694	0.3	0.2	4.842	221.2	2.8	0.0157864
6	4.908	230	20.4	0.0263387	3	0.115064	0.4	0.3	4.908	230.3	2.7	0.0242543
7	5.072	250	22.1	0.393292	5	3.68932	11.8	9.6	5.072	250.4	5	0.41348
8	5.262	280	24.8	0.0973763	7	1.18823	3.8	3.1	5.262	277.7	7	0.0954195
9	5.327	295	26.1	0.530943	3	2.8179	9	7.3	5.327	295.2	3.1	0.520467
10	5.477	330	29.2	0.084598	3	0.497029	1.6	1.3	5.477	329.2	3.2	0.0869229
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.567	351	31.1	0.0549385	4	0.392815	1.3	1	5.567	351	4.1	0.0536862
12	5.794	404	35.8	0.0684016	4	0.446983	1.4	1.2	5.794	404.1	3.6	0.0703563
13	5.846	416	36.8	0.0953052	4	0.49853	1.6	1.3	5.846	416.1	3.5	0.0795319
14	5.905	429	38	0.0249048	9	0.358701	1.1	0.9	5.905	424.3	8.7	0.0232708
15	5.973	444	39.3	0.526875	4	3.92317	12.5	10.2	5.973	444.7	4.2	0.526484
16	6.068	465	41.2	0.347454	4	2.47716	7.9	6.4	6.068	464.7	4.2	0.331081
17	6.145	482	42.7	0.119077	7	1.60724	5.1	4.2	6.145	480.9	7.2	0.126033
18	6.464	552	48.9	0.0785057	8	0.95668	3	2.5	6.464	551.6	7.6	0.0708843
19	6.514	563	49.9	0.0617943	2	0.219045	0.7	0.6	6.514	562.8	2.2	0.0556261
20	6.604	578	51.2	0.0567696	2	0.18068	0.6	0.5	6.604	577.7	1.8	0.0564749
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.673	587	52	0.30998	2	1.31631	4.2	3.4	6.673	587	2.4	0.311086
22	6.781	601	53.2	0.0334522	7	0.38926	1.2	1	6.781	599.6	7.3	0.0302632
23	6.929	625	55.4	0.0216286	3	0.119457	0.4	0.3	6.929	625.1	3	0.0221739
24	7.03	644	57	0.371781	3	1.94455	6.2	5.1	7.03	644.5	3	0.368988
25	7.162	669	59.3	0.315135	5	2.66502	8.5	6.9	7.162	668.9	4.7	0.323252
26	7.289	693	61.4	0.0717506	5	0.573965	1.8	1.5	7.289	692.8	4.5	0.0718904

PeakCounter: 26

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	37	3.3	0.323834	14	7.36607	21.8	16.3	3.5	39.1	14.2	0.293487
2	4.55	179	16	0.263691	6	2.61799	7.8	5.8	4.55	179.4	6.2	0.238848
3	5.2	257	23	0.349	5	3.11497	9.2	6.9	5.2	257.3	5.1	0.34143
4	5.85	412	36.8	0.375767	5	3.30912	9.8	7.3	5.85	412.4	4.9	0.381584
5	6.55	570	50.9	0.293836	5	2.69454	8	6	6.55	568.6	5.4	0.27937
6	6.85	608	54.3	0.104235	10	1.70375	5	3.8	6.85	609.5	9.7	0.0989248
7	7.35	701	62.6	0.253935	6	2.70801	8	6	7.35	700.4	6.2	0.244586
8	8.15	863	77.1	0.0932733	11	1.72011	5.1	3.8	8.15	863	10.6	0.0912248
9	8.45	924	82.6	0.181732	8	2.36139	7	5.2	8.45	924.4	7.6	0.175155
10	8.65	966	86.3	0.169167	6	1.84894	5.5	4.1	8.65	965.2	6.5	0.160518
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1095	97.9	0.33642	8	4.30744	12.8	9.6	9.3	1092.8	8	0.304371

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 123

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 14.500 4.550 9.200 5.200 11.600 5.850 10.300 6.550 8.900 6.850 6.000 7.350 9.400 8.150 5.400 8.450 6.100 8.650 5.000 9.300 13.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator
3.791 2.800 3.878 10.300 4.611 1.200 4.691 1.300 4.845 0.300 4.966 0.400 5.095 11.000 5.252 1.500 5.295 1.500 5.339 9.100 5.482 1.300 5.581 1.700 5.685 0.400 5.755 1.100 5.841 3.400 5.971 10.600 6.054 7.700 6.114 2.300 6.149 3.700 6.439 4.400 6.478 0.600 6.554 1.100 6.623 4.800 6.731 0.800 6.896 0.400 7.005 6.300 7.119 7.600 7.255 2.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 28 Return to Lane Navigator
3.500 19.900 4.550 8.800 5.200 10.700 5.850 9.100 6.550 8.400 6.850 5.200 7.350 8.200 8.150 5.200 8.450 6.600 8.650 5.400 9.300 12.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator
3.500 7.300 3.768 1.200 3.862 6.400 4.420 0.700 4.550 3.900 4.672 1.900 4.826 0.100 4.891 0.500 5.062 6.300 5.200 2.800 5.257 1.700 5.314 5.100 5.464 0.900 5.560 0.900 5.696 2.200 5.740 0 5.793 0.500 5.850 3.600 5.981 7.700 6.083 4.700 6.163 2.900 6.256 0.100 6.499 3.400 6.550 2.000 6.670 0.700 6.738 2.700 6.850 1.300 6.923 0.400 6.975 0.100 7.069 3.700 7.209 5.200 7.350 4.700 8.150 2.700 8.450 2.300 8.650 2.700 9.300 6.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 36 Return to Lane Navigator
3.771 2.600 3.859 10.500 4.579 1.200 4.678 1.300 4.842 0.300 4.908 0.400 5.072 11.800 5.262 3.800 5.327 9.000 5.477 1.600 5.567 1.300 5.794 1.400 5.846 1.600 5.905 1.100 5.973 12.500 6.068 7.900 6.145 5.100 6.464 3.000 6.514 0.700 6.604 0.600 6.673 4.200 6.781 1.200 6.929 0.400 7.030 6.200 7.162 8.500 7.289 1.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 26 Return to Lane Navigator
3.500 21.800 4.550 7.800 5.200 9.200 5.850 9.800 6.550 8.000 6.850 5.000 7.350 8.000 8.150 5.100 8.450 7.000 8.650 5.500 9.300 12.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Lane Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Lane Snapper ---- Go to Display Gel Lane Navigator.