RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Lingcod ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Ophiodon elongatus
FDA Seafood List Common Name: Lingcod
FDA Seafood List Market Name: Lingcod
Unique IEF Gel Code: 19950831gel01
Unique Web Code: lcg01Srr
Tissue Aliquot Code: ophielon I94-001 #02a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 8
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 10
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	28	2.5	0.172442	12	3.49976	16.7	12.1	3.5	32.6	12	0.164848
2	4.55	175	15.5	0.302027	3	1.62074	7.7	5.6	4.55	175.3	3.4	0.270241
3	5.2	258	22.9	0.24655	4	1.59716	7.6	5.5	5.2	258.4	3.8	0.234858
4	5.85	414	36.8	0.447242	4	2.83435	13.5	9.8	5.85	413.9	3.6	0.448322
5	6.55	579	51.4	0.236499	5	1.97454	9.4	6.9	6.55	577.3	5.3	0.208623
6	6.85	618	54.9	0.142823	7	1.48916	7.1	5.2	6.85	618.4	6.9	0.120923
7	7.35	708	62.9	0.284542	6	2.71315	12.9	9.4	7.35	706.9	5.9	0.258879
8	8.15	864	76.7	0.0276665	10	0.429322	2	1.5	8.15	863.8	9.7	0.0250653
9	8.45	924	82.1	0.0405544	7	0.513472	2.4	1.8	8.45	923.8	7.1	0.0405973
10	8.65	962	85.4	0.0429586	6	0.48455	2.3	1.7	8.65	962.6	6.2	0.043911
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1092	97	0.382766	7	3.84225	18.3	13.3	9.3	1089.6	6.5	0.333127

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 9
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 11
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	34	3	0.13691	11	2.41065	4.6	3.8	3.5	36.9	10.6	0.12833
2	4.068	119	10.5	0.693326	5	5.91139	11.3	9.4	4.068	119.8	4.6	0.720061
3	4.55	191	16.9	0.201688	6	1.72905	3.3	2.7	4.55	191.7	6	0.162763
4	4.811	226	20	0.0251103	3	0.173134	0.3	0.3	4.811	224.7	3.3	0.0294252
5	4.931	242	21.4	0.0727191	5	0.640642	1.2	1	4.931	243.8	4.9	0.073465
6	5.11	266	23.5	0.458389	3	2.8234	5.4	4.5	5.11	266.5	3.4	0.466636
7	5.2	278	24.6	0.0646567	4	0.740577	1.4	1.2	5.2	275.6	4.4	0.0952135
8	5.573	363	32.1	0.171445	3	0.815635	1.6	1.3	5.573	363	3	0.154511
9	5.674	386	34.2	0.675635	4	4.33823	8.3	6.9	5.674	387	3.6	0.682732
10	5.767	407	36	0.367006	9	4.46561	8.5	7.1	5.767	404.7	8.7	0.289301
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.85	426	37.7	0.296266	3	1.37547	2.6	2.2	5.85	426.7	2.6	0.296439
12	5.994	458	40.5	0.477166	4	2.88327	5.5	4.6	5.994	458.8	3.6	0.452664
13	6.133	489	43.3	0.138303	8	1.41156	2.7	2.2	6.133	490.5	7.7	0.103357
14	6.353	538	47.6	0.242893	9	3.53269	6.7	5.6	6.353	537.2	9.4	0.212713
15	6.406	550	48.7	0.130935	3	0.518009	1	0.8	6.406	551.3	3.3	0.0897484
16	6.55	582	51.5	0.0688281	8	0.880583	1.7	1.4	6.55	580	7.6	0.0656972
17	6.85	623	55.1	0.41772	3	1.91928	3.7	3	6.85	623	2.6	0.414107
18	7.35	714	63.2	0.328455	4	2.42759	4.6	3.8	7.35	713.3	4.3	0.31538
19	8.15	868	76.8	0.0739173	6	0.578898	1.1	0.9	8.15	866	5.6	0.0584339
20	8.45	927	82	0.0350159	10	0.63451	1.2	1	8.45	929.2	10.2	0.0350041
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.65	975	86.3	0.308438	17	8.86427	16.9	14	8.65	975.4	16.9	0.295927
22	9.3	1097	97.1	0.350194	6	3.27425	6.3	5.2	9.3	1095.7	5.9	0.311335

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 10
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 12
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.03	110	9.7	0.672457	5	5.63239	14.4	11.6	4.03	110.5	4.6	0.696574
2	4.558	184	16.3	0.0605061	8	0.80628	2.1	1.7	4.558	184.6	7.9	0.0573918
3	4.749	209	18.5	0.0381726	4	0.238001	0.6	0.5	4.749	209	3.5	0.0381885
4	4.886	227	20.1	0.048553	7	0.549353	1.4	1.1	4.886	229	7.1	0.0437197
5	5.055	249	22.1	0.532443	4	3.46668	8.9	7.1	5.055	249.4	3.7	0.525197
6	5.546	349	30.9	0.162201	3	0.681469	1.7	1.4	5.546	349.2	2.5	0.15343
7	5.636	370	32.8	0.74017	3	3.92268	10.1	8.1	5.636	370.8	3	0.737313
8	5.722	390	34.5	0.406247	8	4.12865	10.6	8.5	5.722	388.1	8.3	0.2803
9	5.82	413	36.6	0.104791	3	0.501433	1.3	1	5.82	412.7	3	0.0942174
10	5.967	447	39.6	0.497878	4	2.85889	7.3	5.9	5.967	447.8	3.6	0.452422
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.097	477	42.2	0.133973	8	1.32131	3.4	2.7	6.097	479.1	7.8	0.0950607
12	6.322	529	46.9	0.254816	6	2.26422	5.8	4.7	6.322	529	5.6	0.226497
13	6.37	540	47.8	0.147279	5	1.13071	2.9	2.3	6.37	540.7	5	0.128394
14	6.791	614	54.4	0.367258	3	1.90839	4.9	3.9	6.791	614	2.9	0.373929
15	8.66	975	86.4	0.315884	18	9.59379	24.6	19.7	8.66	977.9	17.7	0.306425

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	37	3.3	0.140431	17	4.36188	21	15.6	3.5	39.9	17.5	0.140801
2	4.55	175	15.5	0.264566	4	1.61935	7.8	5.8	4.55	175.1	3.8	0.242569
3	5.2	258	22.9	0.227614	4	1.47751	7.1	5.3	5.2	258.1	3.9	0.215603
4	5.85	414	36.7	0.478084	3	2.573	12.4	9.2	5.85	414.6	2.9	0.492698
5	6.55	581	51.5	0.227181	5	1.70815	8.2	6.1	6.55	580.1	4.8	0.200614
6	6.85	621	55	0.125161	7	1.25013	6	4.5	6.85	621.4	6.7	0.105981
7	7.35	714	63.2	0.275162	6	2.48348	12	8.9	7.35	712.8	5.7	0.246787
8	8.15	874	77.4	0.0327041	10	0.503113	2.4	1.8	8.15	873.1	9.7	0.0293184
9	8.45	933	82.6	0.0579167	7	0.662448	3.2	2.4	8.45	932.1	6.8	0.0547943
10	8.65	971	86	0.0527656	6	0.549142	2.6	2	8.65	970.1	6.3	0.0490368
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1100	97.4	0.372072	6	3.54753	17.1	12.7	9.3	1097.7	6.1	0.329175

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.12	118	10.5	0.688656	5	5.80133	15.3	12.8	4.12	118.1	4.6	0.715967
2	4.759	199	17.6	0.0596569	9	0.872091	2.3	1.9	4.759	198.3	9.5	0.0518198
3	4.987	228	20.2	0.039483	4	0.273052	0.7	0.6	4.987	226.8	4.1	0.0376265
4	5.121	245	21.7	0.0494284	24	2.06115	5.4	4.6	5.121	264.3	24	0.0484542
5	5.253	268	23.8	0.547965	3	2.87777	7.6	6.4	5.253	268	3.2	0.507409
6	5.646	364	32.3	0.169568	2	0.686754	1.8	1.5	5.646	364.1	2.5	0.155776
7	5.731	385	34.1	0.782966	3	4.06595	10.7	9	5.731	385.9	3	0.762649
8	5.809	404	35.8	0.434595	4	2.893	7.6	6.4	5.809	403.5	4.4	0.374512
9	5.892	424	37.6	0.0913098	3	0.488141	1.3	1.1	5.892	425	3.4	0.0817336
10	6.027	456	40.4	0.480249	3	2.39324	6.3	5.3	6.027	456.7	3.3	0.407475
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.14	483	42.8	0.127969	21	2.76174	7.3	6.1	6.14	474	20.9	0.0744671
12	6.35	533	47.3	0.222595	3	1.22519	3.2	2.7	6.35	532.8	3.1	0.22515
13	6.388	542	48	0.138974	5	1.00823	2.7	2.2	6.388	542.3	4.7	0.120256
14	6.798	614	54.4	0.353826	3	1.75501	4.6	3.9	6.798	614.3	2.8	0.354772
15	8.67	977	86.6	0.297858	17	8.87831	23.3	19.7	8.67	977.4	16.9	0.2965

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	42	3.7	0.13892	18	4.72736	21.4	16.3	3.5	40.5	18.3	0.145455
2	4.55	170	15.1	0.233797	4	1.60372	7.3	5.5	4.55	169.9	4	0.225836
3	5.2	252	22.3	0.198516	4	1.45191	6.6	5	5.2	252.3	4.3	0.192702
4	5.85	414	36.7	0.450424	3	2.70848	12.3	9.4	5.85	414.1	3.3	0.465605
5	6.55	580	51.4	0.21125	6	1.84364	8.3	6.4	6.55	578.5	5.7	0.182153
6	6.85	621	55.1	0.119518	7	1.25569	5.7	4.3	6.85	621.6	6.8	0.103635
7	7.35	715	63.4	0.286193	6	2.53371	11.5	8.8	7.35	714.1	5.5	0.259195
8	8.15	876	77.7	0.0332513	10	0.588464	2.7	2	8.15	875.3	10.5	0.0317574
9	8.45	937	83.1	0.0614346	7	0.732583	3.3	2.5	8.45	935.8	7.1	0.0583683
10	8.65	975	86.4	0.0560645	6	0.595394	2.7	2.1	8.65	974.8	6.2	0.0541491
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1102	97.7	0.462104	6	4.06592	18.4	14.1	9.3	1100.7	5.6	0.406905

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK CO SP MK SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 85

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 16.700 4.550 7.700 5.200 7.600 5.850 13.500 6.550 9.400 6.850 7.100 7.350 12.900 8.150 2.000 8.450 2.400 8.650 2.300 9.300 18.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 8 LANE TYPE: pI Markers ACTUAL GEL LANE: 10 PeakCount: 11 Return to Lane Navigator
3.500 4.600 4.068 11.300 4.550 3.300 4.811 0.300 4.931 1.200 5.110 5.400 5.200 1.400 5.573 1.600 5.674 8.300 5.767 8.500 5.850 2.600 5.994 5.500 6.133 2.700 6.353 6.700 6.406 1.000 6.550 1.700 6.850 3.700 7.350 4.600 8.150 1.100 8.450 1.200 8.650 16.900 9.300 6.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 9 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 11 PeakCount: 22 Return to Lane Navigator
4.030 14.400 4.558 2.100 4.749 0.600 4.886 1.400 5.055 8.900 5.546 1.700 5.636 10.100 5.722 10.600 5.820 1.300 5.967 7.300 6.097 3.400 6.322 5.800 6.370 2.900 6.791 4.900 8.660 24.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 10 LANE TYPE: Specimen ACTUAL GEL LANE: 12 PeakCount: 15 Return to Lane Navigator
3.500 21.000 4.550 7.800 5.200 7.100 5.850 12.400 6.550 8.200 6.850 6.000 7.350 12.000 8.150 2.400 8.450 3.200 8.650 2.600 9.300 17.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator
4.120 15.300 4.759 2.300 4.987 0.700 5.121 5.400 5.253 7.600 5.646 1.800 5.731 10.700 5.809 7.600 5.892 1.300 6.027 6.300 6.140 7.300 6.350 3.200 6.388 2.700 6.798 4.600 8.670 23.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 15 Return to Lane Navigator
3.500 21.400 4.550 7.300 5.200 6.600 5.850 12.300 6.550 8.300 6.850 5.700 7.350 11.500 8.150 2.700 8.450 3.300 8.650 2.700 9.300 18.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator

RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Lingcod ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Lingcod ---- Go to Display Gel Lane Navigator.