RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Gray Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lutjanus griseus
FDA Seafood List Common Name: Gray Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19940830gel01
Unique Web Code: gsg01Srr
Tissue Aliquot Code: lutjgris H94-002 #02a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	43	3.8	0.145457	11	2.72716	17.4	11.9	3.5	44.8	11.5	0.133873
2	4.55	187	16.3	0.121434	7	1.31055	8.4	5.7	4.55	187.5	7	0.1057
3	5.2	267	23.3	0.172657	5	1.49207	9.5	6.5	5.2	267.6	5.2	0.16322
4	5.85	418	36.5	0.307161	3	1.59209	10.2	6.9	5.85	418.1	2.9	0.307505
5	6.55	586	51.1	0.15666	6	1.34764	8.6	5.9	6.55	584.8	5.6	0.136866
6	6.85	624	54.5	0.0772554	7	0.82345	5.3	3.6	6.85	624.1	7	0.0660342
7	7.35	718	62.7	0.180164	5	1.41166	9	6.2	7.35	717	4.6	0.171582
8	8.15	883	77.1	0.0658637	10	1.06787	6.8	4.7	8.15	882.3	9.7	0.0618466
9	8.45	943	82.3	0.0876679	7	1.04294	6.7	4.5	8.45	943.1	6.8	0.0866294
10	8.65	984	85.9	0.066204	6	0.628466	4	2.7	8.65	983.5	5.6	0.0629704
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1114	97.2	0.242938	6	2.22264	14.2	9.7	9.3	1112.8	5.6	0.22408

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.493	42	3.7	0.408932	4	3.05935	12.4	10.1	3.493	42.3	4.1	0.41644
2	3.596	56	4.9	0.134937	7	1.72665	7	5.7	3.596	56.6	7.3	0.133542
3	4.202	138	12.1	0.0300513	9	0.449176	1.8	1.5	4.202	139	9	0.0281763
4	4.328	155	13.5	0.0719431	5	0.542344	2.2	1.8	4.328	155.5	4.6	0.0659154
5	4.639	196	17.1	0.391066	4	2.79212	11.3	9.2	4.639	196.4	4	0.394351
6	5.26	279	24.4	0.0989824	5	0.890898	3.6	2.9	5.26	278.5	5.2	0.0959943
7	5.315	292	25.5	0.431521	3	2.54184	10.3	8.4	5.315	292.2	3.3	0.429317
8	5.452	324	28.3	0.0310053	5	0.226824	0.9	0.7	5.452	324.2	4.6	0.0280168
9	5.653	371	32.4	0.0807688	5	0.64491	2.6	2.1	5.653	371.3	5.1	0.0718777
10	5.692	380	33.2	0.0736137	3	0.380163	1.5	1.3	5.692	380.8	2.9	0.0728166
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.773	399	34.8	0.363999	4	2.43804	9.9	8	5.773	400.1	3.8	0.358584
12	5.896	428	37.4	0.236467	4	1.72283	7	5.7	5.896	428	4.4	0.220469
13	5.97	446	39	0.103105	3	0.522474	2.1	1.7	5.97	446	3.1	0.095561
14	6.012	456	39.8	0.0351386	8	0.519058	2.1	1.7	6.012	454.5	8.2	0.0356147
15	6.27	518	45.2	0.121255	4	0.796564	3.2	2.6	6.27	517.9	4.1	0.109548
16	6.365	541	47.2	0.140346	2	0.35673	1.4	1.2	6.365	540.9	1.6	0.123502
17	6.398	549	47.9	0.0844831	7	1.08332	4.4	3.6	6.398	550.2	7.3	0.0841174
18	6.44	559	48.8	0.30334	2	1.00506	4.1	3.3	6.44	559.5	1.9	0.301524
19	6.498	573	50	0.0370597	6	0.294741	1.2	1	6.498	575.1	5.9	0.0279882
20	6.586	590	51.5	0.0703864	2	0.230624	0.9	0.8	6.586	590.2	1.9	0.0701278
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.672	601	52.5	0.201439	2	0.657384	2.7	2.2	6.672	601.5	1.8	0.211591
22	7.034	658	57.5	0.145739	2	0.504543	2	1.7	7.034	657.9	2	0.144425
23	7.74	797	69.6	0.137627	5	1.24874	5.1	4.1	7.74	797.2	5.3	0.132501

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	43	3.8	0.155262	13	3.20231	18.6	13.4	3.5	45.9	12.8	0.141168
2	4.55	183	16	0.184849	5	1.56437	9.1	6.6	4.55	183.1	5.5	0.161617
3	5.2	263	23	0.238779	5	1.87069	10.9	7.8	5.2	262.9	4.7	0.225929
4	5.85	416	36.4	0.290024	3	1.73292	10.1	7.3	5.85	416.6	3.3	0.29307
5	6.55	585	51.1	0.156372	6	1.36578	7.9	5.7	6.55	584.3	5.6	0.138671
6	6.85	623	54.5	0.0702305	6	0.64142	3.7	2.7	6.85	622.7	5.8	0.0624094
7	7.35	716	62.6	0.187504	4	1.40843	8.2	5.9	7.35	715.6	4.4	0.180387
8	8.15	879	76.8	0.0566518	9	0.919581	5.3	3.9	8.15	878.8	9.5	0.0546808
9	8.45	939	82.1	0.0947806	6	1.03815	6	4.3	8.45	939.7	6.3	0.0931465
10	8.65	980	85.7	0.086964	6	0.822384	4.8	3.4	8.65	980	5.6	0.0833114
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1103	96.4	0.231509	7	2.6517	15.4	11.1	9.3	1102.3	7	0.214521

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	42	3.7	0.437135	4	3.50926	9.7	8.4	3.5	42.7	4.5	0.444332
2	3.584	55	4.8	0.184854	7	2.32831	6.5	5.6	3.584	56	7.5	0.176261
3	4.157	144	12.6	0.0424228	10	0.706846	2	1.7	4.157	142.8	10.3	0.0388526
4	4.26	160	14	0.0996747	4	0.701368	1.9	1.7	4.26	160.3	4.4	0.0897004
5	4.55	205	17.9	0.410106	4	3.06285	8.5	7.3	4.55	205.1	4.3	0.401504
6	4.786	233	20.4	0.0180467	9	0.272926	0.8	0.7	4.786	234.3	8.7	0.0176564
7	4.955	253	22.1	0.0164891	2	0.071807	0.2	0.2	4.955	252.1	2.5	0.0162353
8	5.2	282	24.7	0.105001	4	0.573222	1.6	1.4	5.2	281.7	3.6	0.0886487
9	5.245	292	25.5	0.0649701	6	0.791648	2.2	1.9	5.245	290.2	6.3	0.0706688
10	5.303	305	26.7	0.424284	3	2.515	7	6	5.303	304.5	3.2	0.443477
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.442	336	29.4	0.0330887	6	0.309301	0.9	0.7	5.442	335	5.9	0.0296452
12	5.541	358	31.3	0.0339419	8	0.40492	1.1	1	5.541	357.8	8.2	0.0277659
13	5.639	380	33.2	0.0571312	7	0.648279	1.8	1.6	5.639	380.4	6.7	0.0542715
14	5.684	390	34.1	0.0685179	3	0.360743	1	0.9	5.684	390.7	3.3	0.0623042
15	5.774	410	35.8	0.335201	4	2.27305	6.3	5.4	5.774	410.9	4	0.324611
16	5.85	427	37.3	0.149398	4	0.906618	2.5	2.2	5.85	426.5	3.5	0.145721
17	5.899	438	38.3	0.182452	5	1.5086	4.2	3.6	5.899	438.2	4.5	0.188167
18	5.98	456	39.9	0.0864206	4	0.538679	1.5	1.3	5.98	456.2	3.6	0.084905
19	6.303	528	46.2	0.117328	4	0.755029	2.1	1.8	6.303	528.2	4.2	0.101985
20	6.406	551	48.2	0.0976262	2	0.271378	0.8	0.7	6.406	551	1.8	0.087247
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.447	560	49	0.070197	6	0.74556	2.1	1.8	6.447	558.8	5.8	0.0725948
22	6.487	569	49.7	0.274436	2	0.900054	2.5	2.2	6.487	569.3	1.8	0.276355
23	6.55	583	51	0.0738669	7	0.739381	2	1.8	6.55	583.9	6.6	0.0633485
24	6.679	601	52.5	0.107438	2	0.346776	1	0.8	6.679	601.1	1.8	0.107249
25	6.757	612	53.5	0.28214	2	0.80701	2.2	1.9	6.757	612.1	1.6	0.275998
26	6.85	625	54.6	0.0564393	5	0.441083	1.2	1.1	6.85	625	4.7	0.0527081
27	7.092	668	58.4	0.150786	2	0.522786	1.4	1.3	7.092	667.9	2	0.149521
28	7.35	714	62.4	0.150535	4	1.13788	3.2	2.7	7.35	713.9	4.4	0.146827
29	7.804	807	70.5	0.104962	6	1.12641	3.1	2.7	7.804	806.2	6.2	0.101895
30	8.15	878	76.7	0.177695	4	1.67902	4.7	4	8.15	876.5	4.1	0.230619
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.45	933	81.6	0.0762942	16	1.89362	5.2	4.5	8.45	934.5	16.1	0.066516
32	8.65	978	85.5	0.0525646	5	0.471969	1.3	1.1	8.65	977.6	5.3	0.0504412
33	9.3	1104	96.5	0.237977	7	2.75725	7.6	6.6	9.3	1102.6	7	0.222026

PeakCounter: 33

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.487	41	3.6	0.444637	4	3.44628	14.1	10.5	3.487	41.2	4.3	0.454437
2	3.574	54	4.7	0.17891	7	2.03176	8.3	6.2	3.574	54.4	6.6	0.173375
3	4.151	140	12.2	0.0301577	8	0.402282	1.6	1.2	4.151	139.7	7.7	0.0294425
4	4.251	155	13.6	0.0807479	4	0.509367	2.1	1.6	4.251	155.1	3.6	0.0789259
5	4.527	196	17.1	0.413335	4	2.79572	11.4	8.5	4.527	196.5	3.7	0.420796
6	5.218	281	24.6	0.0923192	6	0.924177	3.8	2.8	5.218	280.2	5.6	0.0931074
7	5.28	295	25.8	0.399761	4	2.55184	10.4	7.8	5.28	294.5	3.5	0.408263
8	5.426	328	28.7	0.0267094	5	0.26689	1.1	0.8	5.426	328.7	5.1	0.0297853
9	5.536	353	30.9	0.0202494	8	0.272667	1.1	0.8	5.536	351.5	8.1	0.0188778
10	5.638	376	32.9	0.085702	4	0.602455	2.5	1.8	5.638	376.3	4.1	0.0820373
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.673	384	33.6	0.0466946	3	0.222972	0.9	0.7	5.673	383.3	2.6	0.0490195
12	5.753	402	35.2	0.366141	4	2.19926	9	6.7	5.753	402.7	3.5	0.35277
13	5.881	431	37.7	0.220644	4	1.64684	6.7	5	5.881	430.2	4.5	0.20868
14	5.955	448	39.2	0.0981381	3	0.534864	2.2	1.6	5.955	447.4	3	0.100793
15	5.986	455	39.8	0.0369374	2	0.149725	0.6	0.5	5.986	455	2.3	0.0366799
16	6.029	465	40.7	0.016332	3	0.0928725	0.4	0.3	6.029	465.3	3	0.0175006
17	6.27	520	45.5	0.113882	4	0.705203	2.9	2.2	6.27	519.8	4	0.100131
18	6.371	543	47.5	0.114813	2	0.265562	1.1	0.8	6.371	542.7	1.6	0.0921421
19	6.406	551	48.2	0.0708458	8	0.997801	4.1	3	6.406	551.3	8.2	0.0685132
20	6.454	562	49.2	0.279475	2	0.890325	3.6	2.7	6.454	561.6	1.8	0.28245
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.506	574	50.2	0.0327189	6	0.351471	1.4	1.1	6.506	577.7	6.2	0.0317638
22	6.602	591	51.7	0.0895303	2	0.298707	1.2	0.9	6.602	590.9	1.9	0.0887795
23	6.685	602	52.7	0.246608	2	0.724028	3	2.2	6.685	601.7	1.6	0.254665
24	7.03	657	57.5	0.143679	2	0.493614	2	1.5	7.03	657.2	1.9	0.144323
25	7.745	796	69.6	0.12547	5	1.07124	4.4	3.3	7.745	796.1	5	0.120135

PeakCounter: 25

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	44	3.8	0.165299	11	2.97728	18.8	13.6	3.5	45.4	11	0.152509
2	4.55	194	17	0.148578	5	1.31299	8.3	6	4.55	193.6	5.4	0.135969
3	5.2	272	23.8	0.215015	5	1.70208	10.8	7.8	5.2	272.2	4.6	0.207356
4	5.85	421	36.8	0.311926	3	1.69316	10.7	7.7	5.85	420.8	3	0.316385
5	6.55	585	51.2	0.137608	5	1.12174	7.1	5.1	6.55	583.9	5.3	0.119676
6	6.85	623	54.5	0.0581271	8	0.662514	4.2	3	6.85	623.7	7.5	0.0498239
7	7.35	717	62.7	0.163366	4	1.23274	7.8	5.6	7.35	716	4.4	0.157732
8	8.15	879	76.9	0.0539184	10	0.934754	5.9	4.3	8.15	879.6	9.8	0.0538395
9	8.45	941	82.3	0.0915519	6	1.00023	6.3	4.6	8.45	940.8	6.3	0.0899125
10	8.65	981	85.8	0.0727234	5	0.676772	4.3	3.1	8.65	980.6	5.4	0.0704357
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1102	96.4	0.218433	7	2.50331	15.8	11.5	9.3	1101	6.9	0.205741

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 114

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 17.400 4.550 8.400 5.200 9.500 5.850 10.200 6.550 8.600 6.850 5.300 7.350 9.000 8.150 6.800 8.450 6.700 8.650 4.000 9.300 14.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator
3.493 12.400 3.596 7.000 4.202 1.800 4.328 2.200 4.639 11.300 5.260 3.600 5.315 10.300 5.452 0.900 5.653 2.600 5.692 1.500 5.773 9.900 5.896 7.000 5.970 2.100 6.012 2.100 6.270 3.200 6.365 1.400 6.398 4.400 6.440 4.100 6.498 1.200 6.586 0.900 6.672 2.700 7.034 2.000 7.740 5.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 23 Return to Lane Navigator
3.500 18.600 4.550 9.100 5.200 10.900 5.850 10.100 6.550 7.900 6.850 3.700 7.350 8.200 8.150 5.300 8.450 6.000 8.650 4.800 9.300 15.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 11 Return to Lane Navigator
3.500 9.700 3.584 6.500 4.157 2.000 4.260 1.900 4.550 8.500 4.786 0.800 4.955 0.200 5.200 1.600 5.245 2.200 5.303 7.000 5.442 0.900 5.541 1.100 5.639 1.800 5.684 1.000 5.774 6.300 5.850 2.500 5.899 4.200 5.980 1.500 6.303 2.100 6.406 0.800 6.447 2.100 6.487 2.500 6.550 2.000 6.679 1.000 6.757 2.200 6.850 1.200 7.092 1.400 7.350 3.200 7.804 3.100 8.150 4.700 8.450 5.200 8.650 1.300 9.300 7.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 33 Return to Lane Navigator
3.487 14.100 3.574 8.300 4.151 1.600 4.251 2.100 4.527 11.400 5.218 3.800 5.280 10.400 5.426 1.100 5.536 1.100 5.638 2.500 5.673 0.900 5.753 9.000 5.881 6.700 5.955 2.200 5.986 0.600 6.029 0.400 6.270 2.900 6.371 1.100 6.406 4.100 6.454 3.600 6.506 1.400 6.602 1.200 6.685 3.000 7.030 2.000 7.745 4.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 25 Return to Lane Navigator
3.500 18.800 4.550 8.300 5.200 10.800 5.850 10.700 6.550 7.100 6.850 4.200 7.350 7.800 8.150 5.900 8.450 6.300 8.650 4.300 9.300 15.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Gray Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Gray Snapper ---- Go to Display Gel Lane Navigator.