RFE Fish Species Identification Isoelectric Focusing Tools - Yelloweye Rockfish PAGPlate IEF Gel 01

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Yelloweye Rockfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sebastes ruberrimus
FDA Seafood List Common Name: Yelloweye Rockfish
FDA Seafood List Market Name: Rockfish
Unique IEF Gel Code: 19930817gel01
Unique Web Code: eyg01Srr
Tissue Aliquot Code: sebarube 002 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 1
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	32	2.9	0.045794	16	1.37931	30.6	22.7	3.5	26	16.5	0.0472729
2	4.55	178	15.9	0.0281989	4	0.168853	3.7	2.8	4.55	178.1	3.8	0.0247505
3	5.2	255	22.8	0.0336856	4	0.215802	4.8	3.6	5.2	254.2	3.8	0.0324564
4	5.85	398	35.5	0.0729997	4	0.481154	10.7	7.9	5.85	397.7	3.9	0.0698383
5	6.55	573	51.2	0.0322101	4	0.204923	4.5	3.4	6.55	573	3.7	0.030904
6	7.315	704	62.9	0.00633538	4	0.0388505	0.9	0.6	7.315	702.4	4.1	0.00537879
7	8.15	866	77.3	0.00596594	7	0.0681258	1.5	1.1	8.15	865.2	6.7	0.00577059
8	8.45	924	82.5	0.0112314	7	0.143855	3.2	2.4	8.45	924.7	7.3	0.0111299
9	8.65	967	86.3	0.0089931	6	0.108078	2.4	1.8	8.65	966.1	6.4	0.00953372
10	9.3	1099	98.1	0.209569	5	1.70325	37.7	28.1	9.3	1097.8	4.8	0.198271

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	34	3	0.0530254	22	2.58556	16.8	16.3	3.5	21.8	22.2	0.065623
2	4.149	128	11.4	0.283939	4	2.07915	13.5	13.1	4.149	127.9	4.2	0.281983
3	4.55	186	16.6	0.0260655	5	0.234555	1.5	1.5	4.55	184.2	5.1	0.0261637
4	4.643	196	17.5	0.112308	5	1.12774	7.3	7.1	4.643	196.7	5.4	0.117696
5	5.089	244	21.7	0.03742	14	0.880136	5.7	5.5	5.089	245.7	14	0.0353723
6	5.2	256	22.8	0.0412738	6	0.218046	1.4	1.4	5.2	258	5.5	0.0223391
7	5.741	370	33	0.0143987	8	0.203981	1.3	1.3	5.741	372.7	8.1	0.0142538
8	5.85	393	35	0.0480583	3	0.210733	1.4	1.3	5.85	393	2.8	0.0423726
9	5.882	401	35.7	0.0644111	5	0.611962	4	3.8	5.882	401.7	5.4	0.0636958
10	6.005	432	38.5	0.0580618	7	0.673348	4.4	4.2	6.005	429.8	7.3	0.0521203
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.093	454	40.5	0.0154748	15	0.507246	3.3	3.2	6.093	444.5	14.8	0.0193415
12	6.168	473	42.2	0.0992271	3	0.424682	2.8	2.7	6.168	472.8	2.6	0.0938679
13	6.22	486	43.3	0.0327286	8	0.433206	2.8	2.7	6.22	485.6	7.8	0.0313936
14	6.55	569	50.7	0.0320003	6	0.312037	2	2	6.55	569.7	6	0.0294546
15	7.35	700	62.4	0.0119992	5	0.095981	0.6	0.6	7.35	699.3	5.2	0.0104842
16	8.15	856	76.3	0.00549094	30	0.422789	2.8	2.7	8.15	869.8	30	0.00795127
17	8.45	925	82.4	0.0203663	3	0.042764	0.3	0.3	8.45	921.8	2.7	0.0089771
18	8.599	954	85	0.083376	22	3.25983	21.2	20.5	8.599	952.2	21.6	0.0852483
19	9.3	1101	98.1	0.0980868	6	1.02243	6.7	6.4	9.3	1100.5	6.1	0.0952456

PeakCounter: 19

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.109	121	10.8	0.215753	4	1.39649	23.6	17.5	4.109	121.2	3.6	0.217721
2	4.582	186	16.6	0.144534	3	0.850803	14.4	10.7	4.582	185.5	3.4	0.142231
3	4.93	224	20	0.024681	14	0.562966	9.5	7.1	4.93	228.4	13.7	0.0232563
4	5.744	367	32.8	0.00720963	8	0.0806684	1.4	1	5.744	365.2	7.8	0.00586756
5	5.84	387	34.6	0.0867727	3	0.420427	7.1	5.3	5.84	387.7	2.8	0.0856408
6	5.948	413	36.9	0.0699471	6	0.617998	10.4	7.8	5.948	413.4	5.6	0.0625629
7	6.112	453	40.4	0.0539339	3	0.271639	4.6	3.4	6.112	453.8	2.8	0.0541622
8	6.177	469	41.9	0.021318	4	0.167945	2.8	2.1	6.177	469.5	4.3	0.0219356
9	8.494	929	82.9	0.0452117	22	1.54724	26.2	19.4	8.494	929.3	22.1	0.039565

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	40	3.6	0.0696326	15	1.46144	25.1	17.7	3.5	36.9	14.5	0.0568625
2	4.55	181	16.1	0.0410886	4	0.241042	4.1	2.9	4.55	180.9	4	0.0339789
3	5.2	253	22.5	0.0360249	4	0.24128	4.1	2.9	5.2	252.9	4	0.0341764
4	5.85	387	34.5	0.107456	3	0.519932	8.9	6.3	5.85	386.8	2.8	0.105002
5	6.55	553	49.2	0.0608414	4	0.370992	6.4	4.5	6.55	552.8	3.6	0.0573518
6	7.35	682	60.7	0.0149715	6	0.154543	2.6	1.9	7.35	679.5	6.5	0.0135131
7	8.15	842	75	0.012474	10	0.223372	3.8	2.7	8.15	846.1	10.1	0.0124858
8	8.45	917	81.7	0.0103666	6	0.14499	2.5	1.8	8.45	915.8	6.2	0.0132217
9	8.65	960	85.5	0.00977113	5	0.0862131	1.5	1	8.65	959.3	5.1	0.00948033
10	9.3	1100	98	0.211237	7	2.38984	41	28.9	9.3	1098.9	6.8	0.198829

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	45	4	0.0574896	14	1.13793	8.4	6.9	3.5	42.7	13.9	0.0462549
2	4.127	131	11.7	0.316075	4	2.0678	15.2	12.6	4.127	131.1	3.6	0.321839
3	4.55	189	16.8	0.0581514	5	0.434582	3.2	2.7	4.55	189.5	5.2	0.0475188
4	4.663	202	18	0.221776	3	1.22595	9	7.5	4.663	201.9	3.2	0.213339
5	5.044	246	21.9	0.0454714	13	1.03644	7.6	6.3	5.044	248.8	12.9	0.0454096
6	5.2	264	23.5	0.0530035	3	0.255753	1.9	1.6	5.2	264.6	3.4	0.0430242
7	5.654	359	32	0.0386907	8	0.44908	3.3	2.7	5.654	360.1	7.7	0.0330478
8	5.85	400	35.7	0.272219	3	1.34738	9.9	8.2	5.85	399.3	2.8	0.271422
9	5.934	420	37.4	0.0253806	4	0.133015	1	0.8	5.934	420.9	3.8	0.0196513
10	6.166	475	42.3	0.122083	2	0.61341	4.5	3.7	6.166	473.8	2.3	0.150847
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.234	491	43.8	0.0171613	7	0.188024	1.4	1.1	6.234	492	6.6	0.0161107
12	6.508	556	49.6	0.0297796	11	0.422933	3.1	2.6	6.508	559.4	10.6	0.0224513
13	7.35	691	61.6	0.0120193	4	0.0616471	0.5	0.4	7.35	690.7	3.7	0.00939494
14	7.72	765	68.2	0.00981523	7	0.0996073	0.7	0.6	7.72	766.2	6.5	0.00860143
15	8.15	851	75.8	0.0071532	7	0.11208	0.8	0.7	8.15	852	7.2	0.00873946
16	8.45	913	81.4	0.0162234	4	0.0526795	0.4	0.3	8.45	912.2	4.1	0.00723941
17	8.571	942	84	0.076479	22	2.94646	21.6	18	8.571	945	22	0.0755656
18	9.3	1097	97.8	0.107884	6	1.03599	7.6	6.3	9.3	1095.7	5.8	0.100121

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 8
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.131	134	11.9	0.284462	4	1.88925	17.6	14.6	4.131	133.8	3.7	0.287324
2	4.662	204	18.1	0.166775	5	1.38858	12.9	10.7	4.662	204.9	4.7	0.165896
3	5.015	245	21.8	0.0473519	6	0.339607	3.2	2.6	5.015	244.4	5.6	0.0340064
4	5.144	260	23.1	0.0372136	10	0.724981	6.7	5.6	5.144	256.4	10.4	0.0392115
5	5.663	367	32.7	0.0263528	11	0.459022	4.3	3.5	5.663	369.1	10.6	0.0245186
6	5.834	404	35.9	0.198355	4	1.32138	12.3	10.2	5.834	404.6	3.6	0.206097
7	5.934	427	38	0.0674395	12	1.33247	12.4	10.3	5.934	428.6	12.3	0.0612548
8	6.137	474	42.2	0.167611	2	0.632799	5.9	4.9	6.137	474.1	2.1	0.167688
9	8.557	945	84.1	0.0684372	22	2.65924	24.7	20.5	8.557	942.6	22.2	0.0674596

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 9
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	48	4.3	0.0385624	7	0.459897	15.6	9	3.5	49.6	7.1	0.0366222
2	4.55	190	16.9	0.0241389	6	0.212029	7.2	4.1	4.55	191.8	5.6	0.0213913
3	5.2	266	23.7	0.025557	8	0.302061	10.3	5.9	5.2	268.5	7.7	0.0220335
4	5.85	412	36.7	0.0668936	5	0.548127	18.6	10.7	5.85	412.3	4.6	0.0678199
5	6.55	570	50.7	0.0308833	3	0.177748	6	3.5	6.55	569.7	3.4	0.0291646
6	7.35	699	62.2	0.00423106	8	0.0592637	2	1.2	7.35	699.5	8	0.00420144
7	8.15	867	77.1	0.0077027	11	0.0968026	3.3	1.9	8.15	867.8	10.7	0.00509774
8	8.45	928	82.6	0.0119349	7	0.134576	4.6	2.6	8.45	927.2	7.1	0.0107513
9	8.65	966	85.9	0.00992898	6	0.0958791	3.3	1.9	8.65	967.6	5.7	0.0094406
10	9.3	1101	98	0.0948423	6	0.859086	29.2	16.8	9.3	1099.7	5.5	0.0877997

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 85

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 30.600 4.550 3.700 5.200 4.800 5.850 10.700 6.550 4.500 7.315 0.900 8.150 1.500 8.450 3.200 8.650 2.400 9.300 37.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 1 LANE TYPE: pI Markers ACTUAL GEL LANE: 3 PeakCount: 10 Return to Lane Navigator
3.500 16.800 4.149 13.500 4.550 1.500 4.643 7.300 5.089 5.700 5.200 1.400 5.741 1.300 5.850 1.400 5.882 4.000 6.005 4.400 6.093 3.300 6.168 2.800 6.220 2.800 6.550 2.000 7.350 0.600 8.150 2.800 8.450 0.300 8.599 21.200 9.300 6.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 2 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 4 PeakCount: 19 Return to Lane Navigator
4.109 23.600 4.582 14.400 4.930 9.500 5.744 1.400 5.840 7.100 5.948 10.400 6.112 4.600 6.177 2.800 8.494 26.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 5 PeakCount: 9 Return to Lane Navigator
3.500 25.100 4.550 4.100 5.200 4.100 5.850 8.900 6.550 6.400 7.350 2.600 8.150 3.800 8.450 2.500 8.650 1.500 9.300 41.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 6 PeakCount: 10 Return to Lane Navigator
3.500 8.400 4.127 15.200 4.550 3.200 4.663 9.000 5.044 7.600 5.200 1.900 5.654 3.300 5.850 9.900 5.934 1.000 6.166 4.500 6.234 1.400 6.508 3.100 7.350 0.500 7.720 0.700 8.150 0.800 8.450 0.400 8.571 21.600 9.300 7.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 7 PeakCount: 18 Return to Lane Navigator
4.131 17.600 4.662 12.900 5.015 3.200 5.144 6.700 5.663 4.300 5.834 12.300 5.934 12.400 6.137 5.900 8.557 24.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 8 PeakCount: 9 Return to Lane Navigator
3.500 15.600 4.550 7.200 5.200 10.300 5.850 18.600 6.550 6.000 7.350 2.000 8.150 3.300 8.450 4.600 8.650 3.300 9.300 29.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 9 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

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Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Yelloweye Rockfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Yelloweye Rockfish ---- Go to Display Gel Lane Navigator.