RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
English Sole ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Pleuronectes vetulus
FDA Seafood List Common Name: English Sole
FDA Seafood List Market Name: Sole
Unique IEF Gel Code: 19971126gel02
Unique Web Code: esh06Srr
Tissue Aliquot Code: pleuvetu 001 #2 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	76	8.9	0.0742418	5	0.596164	11.7	7.1	4.55	76.9	4.9	0.0692051
2	5.2	151	17.6	0.0545362	5	0.481855	9.5	5.7	5.2	152	5.4	0.0500205
3	5.85	259	30.3	0.0379015	5	0.345286	6.8	4.1	5.85	258.3	5.2	0.037647
4	6.55	401	46.8	0.0922619	6	0.959966	18.9	11.4	6.55	399.5	6.3	0.0864659
5	6.85	431	50.4	0.0269448	6	0.235621	4.6	2.8	6.85	431.8	6.1	0.0217768
6	7.35	508	59.3	0.0530433	5	0.43538	8.6	5.2	7.35	507.1	5	0.0493917
7	8.15	646	75.5	0.0692673	7	0.872619	17.1	10.4	8.15	645.8	7.5	0.0659713
8	8.45	712	83.2	0.055369	7	0.732626	14.4	8.7	8.45	710.9	7.5	0.0553456
9	8.65	771	90.1	0.0608771	4	0.430632	8.5	5.1	8.65	770.9	4.2	0.057825

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.001	17	2	0.0238634	4	0.162939	0.6	0.5	4.001	17.2	4.4	0.0211234
2	4.331	56	6.5	0.0660683	10	1.15096	4.1	3.3	4.331	56.2	10.3	0.0632747
3	4.44	69	8	0.200212	5	1.53331	5.5	4.5	4.44	68.9	5.1	0.169694
4	4.525	79	9.2	0.375107	5	2.08191	7.4	6	4.525	79.6	4.8	0.24515
5	4.677	97	11.3	0.160616	12	4.75037	16.9	13.8	4.677	89	12.3	0.218145
6	4.854	118	13.7	0.0296113	2	0.073812	0.3	0.2	4.854	119.1	2.3	0.0182797
7	5.04	140	16.3	0.0188269	3	0.106005	0.4	0.3	5.04	139.8	3.2	0.0186163
8	5.124	150	17.5	0.0281478	2	0.119996	0.4	0.3	5.124	150.3	2.3	0.029086
9	5.253	168	19.6	0.0342079	2	0.122227	0.4	0.4	5.253	168.2	2	0.0337535
10	5.307	177	20.6	0.0105071	2	0.0349652	0.1	0.1	5.307	176.6	2	0.00972987
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.396	192	22.4	0.325722	5	2.57453	9.2	7.5	5.396	193.3	4.7	0.305871
12	5.485	207	24.1	0.202945	4	1.41978	5.1	4.1	5.485	207	4.2	0.190705
13	5.562	220	25.6	0.0345715	5	0.301018	1.1	0.9	5.562	219.2	5.1	0.0332297
14	5.651	235	27.4	0.415455	4	2.95177	10.5	8.6	5.651	235.4	4.1	0.406415
15	5.746	251	29.2	0.14693	8	2.09881	7.5	6.1	5.746	249.5	8.5	0.140002
16	5.841	267	31.1	0.0524628	3	0.249413	0.9	0.7	5.841	266.6	2.8	0.0500187
17	5.896	278	32.4	0.202309	3	1.02486	3.6	3	5.896	278	3	0.195923
18	5.987	297	34.6	0.0122503	6	0.108991	0.4	0.3	5.987	299.1	5.9	0.010399
19	6.16	333	38.8	0.0485277	14	1.59903	5.7	4.6	6.16	340.5	14.5	0.0622259
20	6.324	367	42.7	0.221029	15	4.93003	17.5	14.3	6.324	366.8	14.7	0.189794
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.43	389	45.3	0.0999823	3	0.358421	1.3	1	6.43	389.3	2.7	0.0752225
22	6.483	400	46.6	0.0288142	7	0.343804	1.2	1	6.483	397.8	6.5	0.0297392

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	85	9.9	0.0727327	6	0.684601	12.8	8.8	4.55	86.3	5.9	0.0659792
2	5.2	164	19.1	0.0583523	6	0.540546	10.1	6.9	5.2	164.5	5.6	0.0540762
3	5.85	275	32.1	0.0385202	6	0.378705	7.1	4.9	5.85	274.4	5.9	0.0360293
4	6.55	424	49.4	0.124725	6	1.20608	22.6	15.5	6.55	423.5	5.9	0.115702
5	6.85	456	53.1	0.0354655	7	0.361759	6.8	4.6	6.85	456.5	6.5	0.0313141
6	7.35	523	61	0.051105	5	0.39098	7.3	5	7.35	522.3	4.6	0.0478481
7	8.15	649	75.6	0.0592792	9	0.88428	16.6	11.4	8.15	649.8	8.9	0.0560874
8	8.45	720	83.9	0.0482634	7	0.531026	10	6.8	8.45	719.4	6.6	0.0451006
9	8.65	777	90.6	0.0427234	5	0.350472	6.6	4.5	8.65	776.1	4.9	0.0402545

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.038	20	2.3	0.0229388	6	0.220432	1	0.9	4.038	21.6	6	0.0206114
2	4.314	54	6.3	0.0239314	4	0.118048	0.6	0.5	4.314	52.8	3.6	0.0186496
3	4.396	64	7.4	0.0570823	7	0.678877	3.2	2.7	4.396	64.1	7.2	0.0532316
4	4.493	76	8.8	0.089391	2	0.141747	0.7	0.6	4.493	75.1	1.9	0.0414267
5	4.591	88	10.2	0.318859	10	5.26771	25.1	21.1	4.591	89.4	10.1	0.294129
6	4.859	121	14.1	0.023583	4	0.169538	0.8	0.7	4.859	121.4	4.5	0.0213363
7	5.029	142	16.5	0.0115353	4	0.0800232	0.4	0.3	5.029	140.9	4.2	0.010744
8	5.127	154	17.9	0.0126249	4	0.0747136	0.4	0.3	5.127	153.6	3.5	0.0120125
9	5.2	163	19	0.0368218	5	0.297987	1.4	1.2	5.2	163.3	4.7	0.0356836
10	5.243	170	19.8	0.0212836	5	0.168288	0.8	0.7	5.243	171.7	4.9	0.0192474
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.415	198	23	0.131151	6	1.41838	6.8	5.7	5.415	197.1	6.1	0.131793
12	5.507	213	24.8	0.061946	7	0.905828	4.3	3.6	5.507	210.4	7.2	0.0710765
13	5.678	241	28	0.269881	6	2.91248	13.9	11.6	5.678	240.2	6.3	0.262809
14	5.758	254	29.5	0.113353	6	1.20209	5.7	4.8	5.758	254.2	6.1	0.111766
15	5.85	269	31.3	0.0297789	9	0.453506	2.2	1.8	5.85	269.2	9.3	0.027451
16	5.929	286	33.3	0.1356	3	0.718118	3.4	2.9	5.929	285.5	3.1	0.131162
17	6.36	379	44.1	0.152205	15	3.86394	18.4	15.5	6.36	377.8	15.4	0.142013
18	6.471	403	46.9	0.0580744	9	0.369131	1.8	1.5	6.471	410.2	8.6	0.0243463
19	6.55	420	48.8	0.0428872	5	0.295404	1.4	1.2	6.55	420.8	5.1	0.0329785
20	6.85	456	53	0.013512	11	0.234004	1.1	0.9	6.85	455.4	10.9	0.0121545
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.35	520	60.5	0.0286247	5	0.237872	1.1	1	7.35	520.4	4.7	0.0284545
22	8.15	653	75.9	0.049447	11	0.792305	3.8	3.2	8.15	647.2	10.7	0.0417478
23	8.45	730	84.9	0.0200333	8	0.262838	1.3	1.1	8.45	729.1	8	0.0185564
24	8.65	781	90.8	0.0132109	5	0.125955	0.6	0.5	8.65	781.1	5.3	0.0134509

PeakCounter: 24

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.037	20	2.3	0.0237961	5	0.200225	0.7	0.6	4.037	21.4	5.2	0.0216166
2	4.393	63	7.3	0.0701319	9	0.943926	3.3	2.7	4.393	61.8	9.4	0.0568725
3	4.492	75	8.7	0.18191	2	0.300632	1.1	0.9	4.492	74.5	2.3	0.072903
4	4.583	86	10	0.436624	12	8.34134	29.6	23.8	4.583	88.2	12.1	0.389125
5	4.898	124	14.4	0.0331903	3	0.148783	0.5	0.4	4.898	124	3	0.0283197
6	5.08	146	16.9	0.0175988	4	0.121219	0.4	0.3	5.08	145	4.3	0.0158797
7	5.163	156	18.1	0.0252671	3	0.130619	0.5	0.4	5.163	155.4	3	0.0247138
8	5.29	175	20.3	0.0244621	5	0.184116	0.7	0.5	5.29	175.3	4.5	0.0228753
9	5.451	201	23.3	0.229526	8	3.05029	10.8	8.7	5.451	201.8	7.8	0.219304
10	5.544	216	25.1	0.115294	5	0.95202	3.4	2.7	5.544	216.2	5	0.107364
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.717	244	28.3	0.321518	7	3.72248	13.2	10.6	5.717	244	7	0.301318
12	5.81	259	30	0.134331	5	1.23256	4.4	3.5	5.81	257.6	5.4	0.128513
13	5.885	273	31.7	0.0817257	13	1.75062	6.2	5	5.885	273.5	12.5	0.0788647
14	5.94	285	33.1	0.175487	4	1.05669	3.7	3	5.94	285.7	4.4	0.136334
15	6.359	376	43.6	0.197271	18	5.82388	20.7	16.6	6.359	373.5	18	0.182953
16	6.456	397	46.1	0.0680577	3	0.239278	0.8	0.7	6.456	398.4	3.3	0.0403313

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	79	9.2	0.0754263	6	0.670814	10	6.4	4.55	80.1	5.7	0.0668613
2	5.2	156	18.1	0.0825535	5	0.69543	10.4	6.6	5.2	157.1	5.1	0.0772816
3	5.85	260	30.2	0.0340296	5	0.293783	4.4	2.8	5.85	260.6	5	0.0331766
4	6.55	413	47.9	0.119324	7	1.29373	19.4	12.4	6.55	411.5	6.5	0.111975
5	6.85	447	51.9	0.0405729	7	0.416853	6.2	4	6.85	447.7	6.8	0.0347015
6	7.35	514	59.6	0.0776033	4	0.5757	8.6	5.5	7.35	513.9	4.5	0.0727423
7	8.15	652	75.6	0.0771332	8	1.03275	15.5	9.9	8.15	651.3	7.7	0.0759171
8	8.45	721	83.6	0.0781614	8	1.06534	16	10.2	8.45	720	8	0.0754429
9	8.65	775	89.9	0.0918091	4	0.632272	9.5	6	8.65	774.9	4.2	0.0852279

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 89

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 11.700 5.200 9.500 5.850 6.800 6.550 18.900 6.850 4.600 7.350 8.600 8.150 17.100 8.450 14.400 8.650 8.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.001 0.600 4.331 4.100 4.440 5.500 4.525 7.400 4.677 16.900 4.854 0.300 5.040 0.400 5.124 0.400 5.253 0.400 5.307 0.100 5.396 9.200 5.485 5.100 5.562 1.100 5.651 10.500 5.746 7.500 5.841 0.900 5.896 3.600 5.987 0.400 6.160 5.700 6.324 17.500 6.430 1.300 6.483 1.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 22 Return to Lane Navigator
4.550 12.800 5.200 10.100 5.850 7.100 6.550 22.600 6.850 6.800 7.350 7.300 8.150 16.600 8.450 10.000 8.650 6.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.038 1.000 4.314 0.600 4.396 3.200 4.493 0.700 4.591 25.100 4.859 0.800 5.029 0.400 5.127 0.400 5.200 1.400 5.243 0.800 5.415 6.800 5.507 4.300 5.678 13.900 5.758 5.700 5.850 2.200 5.929 3.400 6.360 18.400 6.471 1.800 6.550 1.400 6.850 1.100 7.350 1.100 8.150 3.800 8.450 1.300 8.650 0.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 24 Return to Lane Navigator
4.037 0.700 4.393 3.300 4.492 1.100 4.583 29.600 4.898 0.500 5.080 0.400 5.163 0.500 5.290 0.700 5.451 10.800 5.544 3.400 5.717 13.200 5.810 4.400 5.885 6.200 5.940 3.700 6.359 20.700 6.456 0.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 16 Return to Lane Navigator
4.550 10.000 5.200 10.400 5.850 4.400 6.550 19.400 6.850 6.200 7.350 8.600 8.150 15.500 8.450 16.000 8.650 9.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: English Sole ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
English Sole ---- Go to Display Gel Lane Navigator.