RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
English Sole ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Pleuronectes vetulus
FDA Seafood List Common Name: English Sole
FDA Seafood List Market Name: Sole
Unique IEF Gel Code: 19970213gel05
Unique Web Code: esh04Srr
Tissue Aliquot Code: pleuvetu I94-003 #02a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	93	11.1	0.0491665	4	0.319661	5.1	3.7	4.55	93	3.8	0.047706
2	5.2	181	21.5	0.0713713	5	0.555265	8.9	6.4	5.2	180.9	4.7	0.0671409
3	5.85	295	35.1	0.0835897	4	0.607635	9.7	7	5.85	295.4	4.2	0.0813078
4	6.55	434	51.7	0.127468	6	1.3069	20.9	15.1	6.55	433.4	6.4	0.116054
5	6.85	465	55.4	0.0266422	5	0.245145	3.9	2.8	6.85	466.4	5.3	0.0259948
6	7.35	533	63.5	0.0435932	6	0.41625	6.7	4.8	7.35	532.2	5.8	0.0404541
7	8.15	659	78.5	0.0727214	8	0.928544	14.8	10.7	8.15	658.4	7.5	0.0696661
8	8.45	722	86	0.0929107	7	1.11419	17.8	12.9	8.45	722.1	6.8	0.0923031
9	8.65	775	92.3	0.0828213	5	0.642816	10.3	7.4	8.65	774.3	4.6	0.0790741
10	9.3	820	97.6	0.0196981	3	0.11834	1.9	1.4	9.3	820.4	3.2	0.0208787

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.951	12	1.4	0.0244796	4	0.170789	0.4	0.3	3.951	12.6	4.1	0.0235071
2	4.423	77	9.1	0.265065	5	1.84334	4.4	3.5	4.423	73	5.2	0.198779
3	4.532	92	10.9	0.403931	17	11.8575	28.2	22.6	4.532	96.4	17.2	0.389322
4	4.837	134	15.9	0.0775662	11	1.02216	2.4	1.9	4.837	137.9	11.1	0.0521882
5	5.277	197	23.4	0.0297042	3	0.15405	0.4	0.3	5.277	196.7	3	0.029166
6	5.348	209	24.8	0.074114	3	0.355701	0.8	0.7	5.348	209	2.7	0.0753909
7	5.448	226	26.8	0.503489	4	3.71275	8.8	7.1	5.448	226.8	4.4	0.471465
8	5.531	240	28.5	0.278864	4	2.00752	4.8	3.8	5.531	239.3	4.4	0.259426
9	5.608	253	30	0.0880114	5	0.75834	1.8	1.4	5.608	251.4	4.9	0.0878893
10	5.69	267	31.7	0.582117	4	3.59686	8.5	6.9	5.69	267.6	3.7	0.55388
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.785	283	33.6	0.240181	10	3.76278	8.9	7.2	5.785	282.2	10	0.212649
12	5.865	297	35.3	0.11466	4	0.593262	1.4	1.1	5.865	297.6	3.6	0.0921054
13	5.926	309	36.7	0.308197	4	1.86782	4.4	3.6	5.926	308.7	3.5	0.297766
14	6.016	327	38.8	0.0395256	3	0.189921	0.5	0.4	6.016	326.5	2.7	0.0398206
15	6.157	355	42.2	0.0469327	22	1.46336	3.5	2.8	6.157	354.5	22.4	0.0368855
16	6.313	386	45.8	0.201641	9	2.86006	6.8	5.5	6.313	387.4	9.1	0.177362
17	6.414	406	48.2	0.176742	2	0.587564	1.4	1.1	6.414	406.6	2	0.166466
18	7.216	514	61	0.158326	22	5.2975	12.6	10.1	7.216	511.9	21.5	0.138763

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	94	11.2	0.0324345	7	0.38213	4.5	3.3	4.55	92.3	6.6	0.0324356
2	5.2	185	22	0.0558064	8	0.771991	9.1	6.6	5.2	183.2	8.2	0.0529202
3	5.85	291	34.6	0.0767302	6	0.801629	9.5	6.9	5.85	290.2	6	0.0754991
4	6.55	430	51.1	0.135147	7	1.58119	18.7	13.6	6.55	429.8	7.2	0.123861
5	6.85	463	55	0.0333574	6	0.355945	4.2	3.1	6.85	463.1	6.3	0.0319951
6	7.35	529	62.8	0.0556801	6	0.551055	6.5	4.7	7.35	528.5	6	0.0517819
7	8.15	659	78.3	0.0853695	8	1.1995	14.2	10.3	8.15	659.2	8.2	0.0827661
8	8.45	722	85.7	0.123057	7	1.49168	17.6	12.8	8.45	721.8	6.9	0.122716
9	8.65	773	91.8	0.106603	5	0.835899	9.9	7.2	8.65	772.5	4.7	0.101063
10	9.3	822	97.6	0.0587374	5	0.498742	5.9	4.3	9.3	821.2	5.1	0.0548529

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.953	13	1.5	0.0208867	5	0.168095	0.8	0.6	3.953	13.7	4.8	0.0197723
2	4.468	84	9.9	0.0592595	6	0.613853	2.8	2.3	4.468	83.1	6.1	0.0564198
3	4.606	103	12.2	0.27038	10	4.46189	20.4	17.1	4.606	104.5	9.6	0.260971
4	5.2	185	21.9	0.0397461	5	0.331465	1.5	1.3	5.2	184.8	5	0.0372291
5	5.317	204	24.1	0.0217853	3	0.124599	0.6	0.5	5.317	203.2	3.1	0.0224895
6	5.421	221	26.2	0.25721	5	2.04451	9.3	7.8	5.421	221.3	4.7	0.246462
7	5.507	235	27.8	0.107555	5	0.980595	4.5	3.8	5.507	233.5	5.3	0.10464
8	5.574	246	29.1	0.0236964	5	0.192121	0.9	0.7	5.574	246.2	4.6	0.0234516
9	5.672	262	31	0.392807	4	2.7594	12.6	10.6	5.672	261.8	3.9	0.39601
10	5.752	275	32.5	0.187968	6	1.96581	9	7.5	5.752	274.7	6.2	0.178578
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.85	291	34.4	0.0930996	6	0.987718	4.5	3.8	5.85	291.1	6.2	0.0901169
12	5.905	302	35.7	0.18235	3	1.05169	4.8	4	5.905	301.7	3.3	0.177307
13	5.985	318	37.6	0.0196914	3	0.0907184	0.4	0.3	5.985	318.1	2.6	0.0197836
14	6.145	350	41.4	0.015005	13	0.506398	2.3	1.9	6.145	360.9	13.2	0.0215749
15	6.305	382	45.2	0.162597	12	2.94438	13.5	11.3	6.305	381.1	12	0.138427
16	6.42	405	47.9	0.0741178	2	0.289181	1.3	1.1	6.42	404.9	2.2	0.0725985
17	6.55	431	51	0.0198572	5	0.216987	1	0.8	6.55	428.3	5.1	0.0240585
18	7.35	533	63.1	0.042711	10	0.704818	3.2	2.7	7.35	530.8	10.4	0.0382416
19	8.15	661	78.2	0.0293193	6	0.334271	1.5	1.3	8.15	659.6	6.4	0.0294421
20	8.45	728	86.2	0.0472111	7	0.52371	2.4	2	8.45	727	6.6	0.0450066
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.65	777	92	0.0416294	4	0.28292	1.3	1.1	8.65	776.2	4	0.0401815
22	9.3	824	97.5	0.0175053	11	0.298491	1.4	1.1	9.3	822	11.2	0.0150777

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.016	18	2.1	0.0273624	6	0.258155	0.7	0.6	4.016	19.4	5.7	0.0256615
2	4.462	82	9.7	0.217274	6	1.83098	4.9	4	4.462	80.6	6.2	0.167277
3	4.594	101	11.9	0.390202	16	10.6103	28.3	23	4.594	103.8	15.6	0.384957
4	4.824	134	15.8	0.0702732	9	1.00578	2.7	2.2	4.824	137.4	8.7	0.0653675
5	5.256	197	23.3	0.014571	4	0.108063	0.3	0.2	5.256	196.7	4.2	0.0145817
6	5.337	210	24.8	0.0383374	4	0.256251	0.7	0.6	5.337	210.5	3.7	0.0394256
7	5.455	229	27.1	0.412059	5	3.48634	9.3	7.6	5.455	229.7	4.8	0.407648
8	5.536	242	28.6	0.242337	4	1.67197	4.5	3.6	5.536	241.8	4.2	0.227118
9	5.611	254	30	0.0642926	5	0.589402	1.6	1.3	5.611	253.5	5	0.0660065
10	5.698	268	31.7	0.582336	3	3.3253	8.9	7.2	5.698	267.8	3.3	0.572407
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.772	280	33.1	0.251672	7	3.00492	8	6.5	5.772	279.7	7.2	0.234369
12	5.862	295	34.9	0.0789827	6	0.789094	2.1	1.7	5.862	296.2	6	0.0745477
13	5.922	307	36.3	0.284841	3	1.68533	4.5	3.7	5.922	306.6	3.4	0.279223
14	6.001	323	38.2	0.0245728	4	0.153701	0.4	0.3	6.001	322.7	3.5	0.0245047
15	6.154	354	41.8	0.0537921	24	2.34323	6.2	5.1	6.154	366.6	24	0.0550854
16	6.313	386	45.6	0.186275	9	1.9419	5.2	4.2	6.313	386.9	8.7	0.125851
17	6.421	408	48.2	0.148902	2	0.41376	1.1	0.9	6.421	408.3	1.8	0.132388
18	7.272	526	62.2	0.122527	21	4.0315	10.7	8.8	7.272	524.8	21	0.108437

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	94	11.1	0.0632699	4	0.43303	6.4	3.6	4.55	93.9	4.1	0.0601804
2	5.2	191	22.6	0.0898146	5	0.705638	10.5	5.9	5.2	191	4.8	0.0835165
3	5.85	294	34.8	0.0727165	5	0.597292	8.9	5	5.85	294.1	4.6	0.0734487
4	6.55	437	51.8	0.102172	7	1.19114	17.7	10	6.55	435.8	7.2	0.0935796
5	6.85	471	55.8	0.0219925	5	0.193649	2.9	1.6	6.85	471.9	5.3	0.0204888
6	7.35	540	64	0.0414876	6	0.443	6.6	3.7	7.35	539.2	6.5	0.0387183
7	8.15	662	78.4	0.0699226	7	0.859664	12.8	7.2	8.15	661.4	7.2	0.0678103
8	8.45	726	86	0.0713358	8	0.968054	14.4	8.1	8.45	725.8	7.8	0.0698596
9	8.65	779	92.3	0.0786698	5	0.607441	9	5.1	8.65	778.8	4.6	0.0741959
10	9.3	828	98.1	0.0450974	10	0.729523	10.8	6.1	9.3	825.3	10.2	0.0404011

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 88

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 5.100 5.200 8.900 5.850 9.700 6.550 20.900 6.850 3.900 7.350 6.700 8.150 14.800 8.450 17.800 8.650 10.300 9.300 1.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
3.951 0.400 4.423 4.400 4.532 28.200 4.837 2.400 5.277 0.400 5.348 0.800 5.448 8.800 5.531 4.800 5.608 1.800 5.690 8.500 5.785 8.900 5.865 1.400 5.926 4.400 6.016 0.500 6.157 3.500 6.313 6.800 6.414 1.400 7.216 12.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 18 Return to Lane Navigator
4.550 4.500 5.200 9.100 5.850 9.500 6.550 18.700 6.850 4.200 7.350 6.500 8.150 14.200 8.450 17.600 8.650 9.900 9.300 5.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
3.953 0.800 4.468 2.800 4.606 20.400 5.200 1.500 5.317 0.600 5.421 9.300 5.507 4.500 5.574 0.900 5.672 12.600 5.752 9.000 5.850 4.500 5.905 4.800 5.985 0.400 6.145 2.300 6.305 13.500 6.420 1.300 6.550 1.000 7.350 3.200 8.150 1.500 8.450 2.400 8.650 1.300 9.300 1.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 22 Return to Lane Navigator
4.016 0.700 4.462 4.900 4.594 28.300 4.824 2.700 5.256 0.300 5.337 0.700 5.455 9.300 5.536 4.500 5.611 1.600 5.698 8.900 5.772 8.000 5.862 2.100 5.922 4.500 6.001 0.400 6.154 6.200 6.313 5.200 6.421 1.100 7.272 10.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 18 Return to Lane Navigator
4.550 6.400 5.200 10.500 5.850 8.900 6.550 17.700 6.850 2.900 7.350 6.600 8.150 12.800 8.450 14.400 8.650 9.000 9.300 10.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: English Sole ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
English Sole ---- Go to Display Gel Lane Navigator.