RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Dover Sole ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Microstomus pacificus
FDA Seafood List Common Name: Dover Sole
FDA Seafood List Market Name: Sole
Unique IEF Gel Code: 19970213gel01
Unique Web Code: dvh01Srr
Tissue Aliquot Code: micrpaci I94-003 #04m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	70	8.5	0.050464	4	0.338333	7.2	5	4.55	70.3	3.9	0.0495363
2	5.2	163	19.7	0.0510207	4	0.401548	8.5	5.9	5.2	162.9	4.5	0.0503592
3	5.85	272	32.9	0.0613853	5	0.489549	10.4	7.2	5.85	272.4	4.6	0.0602778
4	6.55	412	49.9	0.0776817	7	0.900765	19.1	13.3	6.55	410.8	7.1	0.071142
5	6.85	446	54	0.0185439	6	0.185991	3.9	2.7	6.85	445.3	5.9	0.0177839
6	7.35	509	61.6	0.037748	5	0.326676	6.9	4.8	7.35	509.5	5.1	0.0361448
7	8.15	638	77.2	0.0703381	7	0.815787	17.3	12	8.15	638.4	6.6	0.0693113
8	8.45	710	86	0.0701025	7	0.848824	18	12.5	8.45	709	6.9	0.0694264
9	8.65	760	92	0.0493397	5	0.410239	8.7	6.1	8.65	759.9	4.8	0.0484622

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.235	22	2.7	0.0122217	6	0.137845	1.1	0.8	4.235	21.5	6.3	0.0124104
2	4.595	74	9	0.31283	4	2.42612	19	14.6	4.595	74.7	4.4	0.314294
3	5.072	143	17.4	0.00889488	2	0.0242299	0.2	0.1	5.072	143.3	1.5	0.00911034
4	5.389	193	23.5	0.0344118	4	0.227332	1.8	1.4	5.389	192.3	3.8	0.0339823
5	5.592	227	27.6	0.0628655	2	0.221014	1.7	1.3	5.592	226.3	2	0.0633182
6	5.628	233	28.3	0.307102	3	1.57832	12.3	9.5	5.628	232.9	3	0.299566
7	5.706	246	29.9	0.080778	4	0.492609	3.9	3	5.706	246.4	3.5	0.07858
8	5.766	256	31.1	0.0463244	3	0.27625	2.2	1.7	5.766	255.5	3.3	0.0469599
9	5.814	264	32.1	0.057205	6	0.87954	6.9	5.3	5.814	269	6.3	0.0792518
10	5.88	276	33.5	0.506145	4	3.01703	23.6	18.2	5.88	275.9	4	0.420675
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.011	302	36.7	0.0279941	8	0.311141	2.4	1.9	6.011	301.3	7.7	0.0228687
12	6.177	335	40.7	0.0426542	8	0.527658	4.1	3.2	6.177	335.6	7.8	0.0380623
13	6.293	358	43.5	0.0841837	8	1.12021	8.8	6.8	6.293	357	8	0.0792593
14	6.424	384	46.7	0.133004	6	1.31606	10.3	7.9	6.424	384	5.7	0.130612
15	6.612	416	50.5	0.0523285	3	0.235878	1.8	1.4	6.612	416.4	2.5	0.053038

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	69	8.4	0.0604271	4	0.422358	8	5.5	4.55	69.6	4.1	0.0581362
2	5.2	164	19.9	0.062298	4	0.445201	8.4	5.8	5.2	163.8	4.2	0.0593021
3	5.85	272	33	0.0757593	4	0.597959	11.3	7.7	5.85	271.5	4.5	0.0756636
4	6.55	410	49.7	0.0930462	7	1.01814	19.3	13.2	6.55	410.1	6.8	0.0844123
5	6.85	444	53.8	0.0157175	7	0.19238	3.6	2.5	6.85	446.3	7.1	0.0153795
6	7.35	509	61.7	0.0419964	5	0.33022	6.2	4.3	7.35	508.7	4.6	0.0401879
7	8.15	638	77.3	0.0807216	7	1.01678	19.2	13.1	8.15	637.5	7.3	0.0785903
8	8.45	708	85.8	0.0723062	6	0.785066	14.9	10.1	8.45	708	6.1	0.072311
9	8.65	760	92.1	0.0620033	4	0.476563	9	6.2	8.65	760	4.5	0.0599975

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.238	20	2.4	0.0128818	5	0.103371	0.7	0.6	4.238	19.7	4.8	0.0121377
2	4.594	77	9.3	0.288596	5	2.44297	17.4	14.1	4.594	76.6	4.8	0.287563
3	5.037	148	17.9	0.00711933	2	0.0239859	0.2	0.1	5.037	147.8	1.8	0.00735229
4	5.2	174	21.1	0.0301127	3	0.181586	1.3	1	5.2	173.2	3.4	0.0298077
5	5.362	199	24.1	0.0298033	3	0.161319	1.2	0.9	5.362	198.9	3.1	0.0295062
6	5.576	232	28.1	0.065112	4	0.380291	2.7	2.2	5.576	232.6	4.2	0.0507058
7	5.615	238	28.8	0.210717	3	0.942857	6.7	5.4	5.615	238.6	2.7	0.199066
8	5.699	251	30.4	0.0485116	4	0.302105	2.2	1.7	5.699	251	3.6	0.0477289
9	5.764	261	31.6	0.0303957	3	0.178965	1.3	1	5.764	260.7	3.4	0.0301366
10	5.822	270	32.7	0.0502036	7	0.629863	4.5	3.6	5.822	273.7	6.5	0.0544401
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.889	282	34.2	0.423031	5	3.09721	22.1	17.9	5.889	281.6	4.6	0.382174
12	6.033	310	37.6	0.0151035	8	0.156721	1.1	0.9	6.033	312.7	7.9	0.0111457
13	6.207	344	41.7	0.0507043	10	0.795976	5.7	4.6	6.207	344.9	9.9	0.0454968
14	6.314	365	44.2	0.105566	9	1.53892	11	8.9	6.314	365.7	8.9	0.0972914
15	6.448	391	47.4	0.125958	6	1.28116	9.1	7.4	6.448	390.4	5.8	0.12425
16	6.55	411	49.8	0.0118688	3	0.067054	0.5	0.4	6.55	411	3.3	0.0115215
17	6.659	423	51.3	0.0363188	2	0.133655	1	0.8	6.659	423.1	2.1	0.0360892
18	6.85	444	53.8	0.0086774	8	0.103601	0.7	0.6	6.85	445.6	8.2	0.00708834
19	7.35	509	61.7	0.0237696	5	0.211544	1.5	1.2	7.35	508.5	5.4	0.0222708
20	8.15	642	77.8	0.0165152	18	0.694815	5	4	8.15	629.6	18	0.0217786
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	714	86.5	0.0351532	6	0.365018	2.6	2.1	8.45	714.4	6.2	0.0333946
22	8.65	764	92.6	0.0226612	6	0.212809	1.5	1.2	8.65	764.2	5.6	0.0212512

PeakCounter: 22

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.23	18	2.2	0.0134173	6	0.139347	0.8	0.7	4.23	17.4	5.9	0.0134313
2	4.576	71	8.6	0.302262	5	2.72412	16.5	13.1	4.576	71	5	0.305229
3	4.994	135	16.4	0.0185524	16	0.683246	4.1	3.3	4.994	124.6	16	0.0241493
4	5.301	183	22.2	0.0289889	5	0.257331	1.6	1.2	5.301	183.8	5.1	0.0286198
5	5.509	217	26.3	0.0110005	3	0.0588947	0.4	0.3	5.509	216.8	3.5	0.00950006
6	5.54	222	26.9	0.0583994	3	0.277887	1.7	1.3	5.54	221.8	2.7	0.0577333
7	5.583	229	27.8	0.249935	4	1.59248	9.6	7.6	5.583	229.5	3.6	0.252005
8	5.681	245	29.7	0.0586442	4	0.437398	2.6	2.1	5.681	245.4	4.2	0.0586574
9	5.748	256	31.1	0.0541331	4	0.361337	2.2	1.7	5.748	255.4	4.1	0.0499694
10	5.809	266	32.3	0.0734729	8	1.2049	7.3	5.8	5.809	270.3	8.4	0.0810017
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.877	278	33.7	0.392582	5	3.2228	19.5	15.5	5.877	278.1	4.9	0.368175
12	6.02	306	37.1	0.0220404	8	0.30387	1.8	1.5	6.02	307.8	8.1	0.0210564
13	6.183	338	41	0.0625825	8	0.889151	5.4	4.3	6.183	338.6	8.4	0.0595954
14	6.295	360	43.7	0.134303	11	2.37848	14.4	11.4	6.295	360	10.7	0.124911
15	6.423	385	46.7	0.166022	6	1.86198	11.3	8.9	6.423	385.4	6.4	0.164768
16	6.595	415	50.4	0.0338565	2	0.147852	0.9	0.7	6.595	415	2.4	0.0340491

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	68	8.3	0.0575142	4	0.404431	9.2	6.4	4.55	68.7	4.1	0.0562604
2	5.2	163	19.8	0.0637493	4	0.4672	10.6	7.4	5.2	162.6	4.3	0.0615581
3	5.85	275	33.4	0.0708262	5	0.567747	12.9	9	5.85	275.3	4.6	0.0698789
4	6.55	413	50.1	0.0693497	8	0.888449	20.2	14	6.55	412	7.7	0.0646944
5	6.85	446	54.1	0.012516	5	0.111195	2.5	1.8	6.85	447.1	5.3	0.0117985
6	7.35	512	62.1	0.027454	5	0.233502	5.3	3.7	7.35	511.3	5.1	0.0260273
7	8.15	636	77.2	0.0713532	6	0.760441	17.3	12	8.15	636.6	6.3	0.0683706
8	8.45	709	86	0.0463172	7	0.595547	13.5	9.4	8.45	709.4	7.3	0.0457826
9	8.65	762	92.5	0.0477731	5	0.378448	8.6	6	8.65	761.2	4.8	0.0449233

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 80

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 7.200 5.200 8.500 5.850 10.400 6.550 19.100 6.850 3.900 7.350 6.900 8.150 17.300 8.450 18.000 8.650 8.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.235 1.100 4.595 19.000 5.072 0.200 5.389 1.800 5.592 1.700 5.628 12.300 5.706 3.900 5.766 2.200 5.814 6.900 5.880 23.600 6.011 2.400 6.177 4.100 6.293 8.800 6.424 10.300 6.612 1.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 15 Return to Lane Navigator
4.550 8.000 5.200 8.400 5.850 11.300 6.550 19.300 6.850 3.600 7.350 6.200 8.150 19.200 8.450 14.900 8.650 9.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.238 0.700 4.594 17.400 5.037 0.200 5.200 1.300 5.362 1.200 5.576 2.700 5.615 6.700 5.699 2.200 5.764 1.300 5.822 4.500 5.889 22.100 6.033 1.100 6.207 5.700 6.314 11.000 6.448 9.100 6.550 0.500 6.659 1.000 6.850 0.700 7.350 1.500 8.150 5.000 8.450 2.600 8.650 1.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 22 Return to Lane Navigator
4.230 0.800 4.576 16.500 4.994 4.100 5.301 1.600 5.509 0.400 5.540 1.700 5.583 9.600 5.681 2.600 5.748 2.200 5.809 7.300 5.877 19.500 6.020 1.800 6.183 5.400 6.295 14.400 6.423 11.300 6.595 0.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 16 Return to Lane Navigator
4.550 9.200 5.200 10.600 5.850 12.900 6.550 20.200 6.850 2.500 7.350 5.300 8.150 17.300 8.450 13.500 8.650 8.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Dover Sole ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Dover Sole ---- Go to Display Gel Lane Navigator.