Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Coney(*)  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Cephalopholis(*) fulva(*)
FDA Seafood List Common Name: Coney(*)
FDA Seafood List Market Name: Grouper(*)
Unique IEF Gel Code: 19971029gel08
Unique Web Code: cyh01Srr
Tissue Aliquot Code: cephfulv I96-002 #03m Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
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Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.557690.11191750.8816611.78.74.5577.150.0986919
25.216419.30.088264140.67549796.65.2164.64.50.0856138
35.8528233.30.087487640.5459827.35.45.85282.13.70.0824629
46.5542950.60.13276461.3552818.113.36.55427.66.10.125192
56.8546354.60.037980360.3351924.53.36.85462.66.20.0305205
67.3553663.20.060029740.3973665.33.97.35535.53.80.0590453
78.1565877.60.054344780.78192510.47.78.15658.78.50.0521989
88.4572285.10.10546381.3993518.613.78.45721.97.60.104106
98.6577391.20.096036940.683559.16.78.65772.14.20.0920147
109.381996.60.067097340.44864.49.3818.83.90.0654573
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.05131.50.004861810.01311470.10.14.05141.50.0050931
24.16273.20.40716543.0872317.714.54.1627.54.20.412567
34.562789.20.051179930.3145221.81.54.56277.93.40.0525213
44.76710412.30.013166440.08927590.50.44.767103.74.10.0122247
54.97913115.50.01532380.1969651.10.94.979133.37.80.0141727
65.082144170.070080730.396152.31.95.0821443.40.0655665
75.22716419.40.49350222.1406312.310.15.227163.62.40.495873
85.3819222.70.03360130.1536190.90.75.38191.72.60.0329396
95.50621525.40.10454320.4418342.52.15.5062152.40.103013
105.65324228.60.035530130.2116111.215.653241.93.40.0351274
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.76826331.10.58159333.2514618.715.35.768263.13.30.564261
125.84527732.70.29968342.0592111.89.75.845277.24.10.280361
135.90629034.30.017977420.07780250.40.45.906288.820.0215859
145.99430936.50.17705641.278197.365.994308.74.20.170011
156.03131737.50.21759741.207986.95.76.031317.53.80.179948
166.15234340.50.042311260.4205142.426.152343.25.70.0418388
176.33438245.20.039208850.2988271.71.46.334382.74.50.0373211
186.38539346.50.056911130.35657721.76.3853933.50.0582206
196.41740047.30.2004231.119966.45.36.417400.43.40.186426
206.47841348.80.069278220.3016721.71.46.478413.32.40.0701843
PeakCounter: 20
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55799.30.080188360.7748989.77.14.5580.15.80.0758276
25.215618.40.057176160.5502996.955.2156.95.70.0545273
35.8527632.60.093497540.6371385.85.85275.840.0903548
46.5543050.80.14671161.517471913.96.55429.56.20.137875
56.8546254.60.032614550.2902143.62.76.854635.30.0309293
67.3553262.90.051793730.2803713.52.67.35532.43.10.0505852
78.15660780.0599028111.112613.910.28.15659.610.90.0575024
88.4572185.20.11645571.4714818.413.58.45721.17.30.11437
98.65770910.10324240.7872729.87.28.65769.94.50.099552
109.381696.50.081951140.581047.35.39.3815.74.20.0786459
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.035222.60.036704450.3458431.61.44.035235.20.0377589
24.153364.30.44416343.390861613.34.15336.44.30.445437
34.55839.80.078584350.6812443.22.74.5584.150.0773423
44.78611113.10.011707930.06402080.30.34.786110.830.0119564
55.02313916.40.024847950.1963780.90.85.023138.24.70.0236257
65.10714917.60.069999240.494562.31.95.107148.84.40.0634575
75.23916719.70.48502232.4461911.69.65.239166.82.90.479676
85.38319322.80.02375330.121880.60.55.38319330.0228766
95.5121625.50.091678230.4119321.91.65.51215.62.50.0918708
105.65424228.60.040661260.3633631.71.45.654242.25.70.0356551
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.77626431.20.5208443.1942415.112.55.776264.53.60.499992
125.858279330.25611352.064619.88.15.858278.65.10.226278
136.012313370.12776451.124175.34.46.012312.35.20.122682
146.05332238.10.15083820.5900952.82.36.053321.72.20.149516
156.07232638.50.071410330.3200921.51.36.072326.72.50.0717091
166.19935441.80.025127880.3124031.51.26.199352.47.80.0225918
176.39139646.80.030661550.2543871.216.391395.44.80.0301218
186.43240547.90.061987130.3063411.41.26.432404.33.10.0553971
196.46341248.70.19028541.250455.94.96.463411.63.90.179099
206.51842450.10.10797650.8831114.23.56.518424.65.40.0925337
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.5543150.90.05602840.20699810.86.55431.73.80.0309225
226.8546254.60.025164150.2226251.10.96.85461.65.20.0240147
237.3552962.50.0429575100.6190652.92.47.35529.2100.0350923
248.4572685.80.038528190.6006192.82.48.45724.390.0377235
258.6577291.30.048601640.3638261.71.48.65771.14.40.0465878
269.381796.60.033543760.3234131.51.39.3816.76.40.0283607
PeakCounter: 26
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.039141.70.014246210004.039810
24.14273.20.36930453.190991814.94.1427.54.80.375217
34.55809.50.039869240.3067771.71.44.5579.44.40.0396021
44.75910712.60.011194370.1259410.70.64.759106.47.20.0098504
54.99913816.30.009817430.05052740.30.24.999136.530.0095647
65.07614817.50.05146430.2915711.61.45.076147.53.10.053022
75.20616519.50.4450132.12131129.95.206165.42.70.439512
85.34719022.50.022161330.1165160.70.55.347190.72.80.0234976
95.47221225.10.081985630.4554722.62.15.4722123.10.0825025
105.63124028.40.032815260.3383081.91.65.631239.76.30.030465
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.76726431.20.50759343.5511520.116.65.767264.540.495918
125.86128133.20.27928742.0107611.49.45.861281.24.40.260768
136.007313370.13568351.187346.75.56.007312.35.10.130313
146.03932037.80.20903220.6731793.83.16.039320.31.90.197676
156.06632638.50.079426130.3787422.11.86.066325.42.50.0840595
166.18935341.70.034099380.4759242.72.26.189352.68.50.0316195
176.37739446.60.042815950.35771721.76.377394.450.040696
186.42740547.90.078989830.3063941.71.46.427403.22.70.0632775
196.4541048.50.20584841.320627.56.26.45410.43.80.193633
206.509423500.081214730.4521362.62.16.509422.53.50.0738346
PeakCounter: 20
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55779.10.10312950.75953210.87.84.5577.44.50.0943602
25.216819.90.079689750.6788479.675.2168.35.10.0744615
35.8528033.10.078581740.4866846.955.85279.73.70.0743252
46.5543351.20.12517361.2339817.512.76.55431.960.116705
56.8546755.20.022533880.270953.82.86.85468.37.70.0199064
67.3553463.10.051525140.3391594.83.57.35534.13.90.0495755
78.1566178.10.0567664100.97065813.7108.15661.610.30.0531658
88.4572585.70.086003181.1476516.211.88.4572580.0805562
98.6577191.10.077878550.619248.86.48.65770.34.80.0734989
109.381896.70.078666640.5559477.95.79.3817.24.20.0752036
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 6.85 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 96
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 20 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 26 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 20 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln