RFE Fish Species Identification Isoelectric Focusing Tools - Caribbean Red Snapper Phast IEF Gel 02

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Caribbean Red Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lutjanus purpureus
FDA Seafood List Common Name: Caribbean Red Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19970207gel04
Unique Web Code: csh02Srr
Tissue Aliquot Code: lutjpurp H94-002 #03a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	95	11.2	0.0869849	4	0.554304	6	4.4	4.55	95	3.7	0.0844067
2	5.2	191	22.6	0.139629	4	0.896883	9.7	7	5.2	190.7	3.8	0.132748
3	5.85	303	35.8	0.107282	4	0.789179	8.5	6.2	5.85	303.2	4.3	0.102539
4	6.55	425	50.2	0.145442	7	1.62458	17.5	12.8	6.55	424.6	6.9	0.133721
5	6.85	458	54.1	0.0353939	5	0.263148	2.8	2.1	6.85	458	4.5	0.0327051
6	7.35	523	61.7	0.0782389	5	0.640979	6.9	5	7.35	522.5	4.9	0.0737015
7	8.15	645	76.2	0.118046	8	1.54606	16.7	12.1	8.15	643.8	7.7	0.113617
8	8.45	714	84.3	0.115345	8	1.65133	17.8	13	8.45	713.4	8.3	0.112661
9	8.65	771	91	0.0948144	5	0.874402	9.4	6.9	8.65	770	5.4	0.0921341
10	9.3	830	98	0.0467125	6	0.438745	4.7	3.4	9.3	828.8	5.6	0.0439647

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.088	24	2.9	0.221011	7	2.79234	6.1	5.4	4.088	24.6	7.1	0.221201
2	4.326	60	7.1	0.464582	5	3.9869	8.7	7.7	4.326	60.5	4.9	0.45703
3	4.464	81	9.6	0.0302441	18	0.612606	1.3	1.2	4.464	76.2	18	0.0192018
4	4.755	125	14.8	0.0186112	5	0.13884	0.3	0.3	4.755	123.7	4.6	0.017134
5	4.847	139	16.5	0.0135083	9	0.182802	0.4	0.4	4.847	142.1	8.6	0.0120274
6	5.065	172	20.4	0.292973	6	2.72495	5.9	5.3	5.065	172.8	6.2	0.246201
7	5.26	203	24.1	0.0595688	6	0.593395	1.3	1.1	5.26	201.1	6.1	0.0547706
8	5.346	218	25.9	0.537018	3	2.43291	5.3	4.7	5.346	218.3	3	0.456128
9	5.461	238	28.3	0.0454772	8	1.99963	4.3	3.9	5.461	224.9	7.9	0.142085
10	5.592	261	31	0.0568079	6	0.557416	1.2	1.1	5.592	260.4	5.7	0.0549943
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.678	276	32.8	0.0543728	6	0.630853	1.4	1.2	5.678	276.1	6.4	0.0553528
12	5.902	315	37.4	0.0288967	4	0.226659	0.5	0.4	5.902	315.2	4.4	0.0292939
13	6.023	336	39.9	0.527849	4	3.17819	6.9	6.2	6.023	336.5	3.6	0.504297
14	6.115	352	41.8	0.316459	4	1.88192	4.1	3.6	6.115	352.1	3.6	0.29581
15	6.201	367	43.6	0.301823	4	2.17937	4.7	4.2	6.201	366.1	4.1	0.30269
16	6.271	379	45	0.0403613	3	0.191123	0.4	0.4	6.271	379.4	2.6	0.0407185
17	6.345	392	46.6	0.101401	3	0.524346	1.1	1	6.345	392.3	3.1	0.0951405
18	6.472	414	49.2	0.0847647	4	0.678343	1.5	1.3	6.472	413.8	4.4	0.0864653
19	6.501	419	49.8	0.131769	3	0.535171	1.2	1	6.501	419.4	2.6	0.116738
20	6.619	435	51.7	0.460319	3	2.74628	6	5.3	6.619	434.9	3.4	0.455494
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.702	444	52.7	0.364939	3	1.70815	3.7	3.3	6.702	444.3	2.6	0.369013
22	6.832	458	54.4	0.133951	5	1.14901	2.5	2.2	6.832	457	5	0.129599
23	6.944	472	56.1	0.0361159	4	0.26491	0.6	0.5	6.944	473.4	4.1	0.036515
24	7.053	486	57.7	0.0244538	2	0.0838935	0.2	0.2	7.053	486.6	1.8	0.0257611
25	7.147	498	59.1	0.0386902	9	0.612498	1.3	1.2	7.147	503.4	9.1	0.0377849
26	7.248	511	60.7	0.322841	3	1.37169	3	2.7	7.248	510.6	2.5	0.304322
27	7.643	567	67.3	0.0152988	3	0.0723733	0.2	0.1	7.643	566.8	2.8	0.0143726
28	7.731	580	68.9	0.0465091	8	0.623234	1.4	1.2	7.731	580.6	7.6	0.0460605
29	7.799	590	70.1	0.339713	3	1.44584	3.1	2.8	7.799	589.7	2.7	0.302278
30	7.915	607	72.1	0.223545	11	5.72493	12.4	11.1	7.915	613.7	10.7	0.302402
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.565	743	88.2	0.131568	18	4.13472	9	8	8.565	743.3	18	0.1296

PeakCounter: 31

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	93	11.1	0.0466961	5	0.448385	6.9	4.9	4.55	92.6	5.4	0.0470387
2	5.2	194	23.1	0.0457352	10	0.782636	12	8.6	5.2	191.3	9.6	0.0458204
3	5.85	309	36.8	0.0777467	6	0.828923	12.8	9.1	5.85	309.3	6.1	0.0769901
4	6.55	430	51.2	0.134866	6	1.35122	20.8	14.8	6.55	429.4	6.3	0.121405
5	6.85	462	55	0.0285301	4	0.211866	3.3	2.3	6.85	462.6	4.4	0.0270841
6	7.35	525	62.5	0.048523	5	0.414567	6.4	4.5	7.35	524.7	5.2	0.0446359
7	8.15	638	76	0.0865824	7	1.0296	15.8	11.3	8.15	637.6	7	0.0835507
8	8.45	705	83.9	0.0621847	8	0.852384	13.1	9.4	8.45	703.9	7.8	0.0613462
9	8.65	765	91.1	0.0491805	6	0.490464	7.5	5.4	8.65	764.3	5.8	0.0481156
10	9.3	824	98.1	0.0143038	3	0.0865821	1.3	0.9	9.3	824.3	3.3	0.0146425

PeakCounter: 10

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.082	27	3.2	0.119695	7	1.50471	4.7	4.6	4.082	27.2	7.2	0.118337
2	4.305	59	7	0.339433	5	2.87417	8.9	8.7	4.305	59	4.9	0.330155
3	4.403	73	8.7	0.0068472	14	0.300326	0.9	0.9	4.403	65.8	14.2	0.0119301
4	4.55	94	11.2	0.0366461	4	0.238796	0.7	0.7	4.55	94.3	3.9	0.0349469
5	4.725	119	14.1	0.0114098	5	0.0961708	0.3	0.3	4.725	119.1	4.8	0.0111911
6	4.865	139	16.5	0.0173869	6	0.163944	0.5	0.5	4.865	138.8	5.6	0.0164042
7	5.039	164	19.5	0.143127	6	1.35579	4.2	4.1	5.039	164.7	5.6	0.137482
8	5.2	187	22.2	0.0478796	12	0.935994	2.9	2.8	5.2	186.7	12.1	0.043651
9	5.349	214	25.4	0.24022	6	2.49074	7.7	7.6	5.349	212.8	5.9	0.239066
10	5.464	235	27.9	0.0205671	12	0.578125	1.8	1.8	5.464	225.8	11.8	0.0276998
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.602	260	30.9	0.0322536	7	0.389541	1.2	1.2	5.602	259	7	0.0311993
12	5.674	273	32.4	0.0473397	6	0.509706	1.6	1.6	5.674	272.9	6.2	0.0466643
13	5.85	305	36.2	0.0616226	5	0.553962	1.7	1.7	5.85	305.4	5.3	0.0590841
14	6.013	334	39.7	0.46235	3	2.65628	8.3	8.1	6.013	334.7	3.3	0.455267
15	6.115	352	41.8	0.211583	4	1.49028	4.6	4.5	6.115	351.5	4.2	0.202544
16	6.205	368	43.7	0.191439	5	1.6889	5.3	5.1	6.205	368	5	0.192407
17	6.295	384	45.6	0.0181128	3	0.112401	0.3	0.3	6.295	384.9	3.4	0.0187871
18	6.385	400	47.5	0.041756	3	0.241735	0.8	0.7	6.385	399.8	3.5	0.0393896
19	6.514	423	50.2	0.052925	5	0.438258	1.4	1.3	6.514	423.1	4.6	0.0538107
20	6.542	428	50.8	0.100558	3	0.428728	1.3	1.3	6.542	428.6	2.8	0.087183
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.677	443	52.6	0.462801	3	2.42075	7.5	7.4	6.677	443.3	3	0.462523
22	6.76	452	53.7	0.374689	2	1.51558	4.7	4.6	6.76	451.7	2.3	0.379323
23	6.86	463	55	0.0538294	5	0.455811	1.4	1.4	6.86	462.3	4.9	0.0524155
24	6.985	479	56.9	0.0162089	4	0.108627	0.3	0.3	6.985	479.2	3.8	0.0160917
25	7.071	490	58.2	0.0125408	2	0.0438403	0.1	0.1	7.071	490.1	1.9	0.0129862
26	7.243	512	60.8	0.189156	3	0.963812	3	2.9	7.243	512.2	2.9	0.186712
27	7.786	589	70	0.197132	5	1.67734	5.2	5.1	7.786	588.4	5.1	0.187375
28	7.944	612	72.7	0.122684	15	4.2685	13.3	13	7.944	619	15.2	0.158627
29	8.45	707	84	0.025592	6	0.282749	0.9	0.9	8.45	706.9	6.1	0.026059
30	8.555	738	87.6	0.0257951	13	0.546868	1.7	1.7	8.555	736.6	13.5	0.0228588
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.65	766	91	0.047615	11	0.82897	2.6	2.5	8.65	764.5	11.1	0.0419705

PeakCounter: 31

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.078	28	3.3	0.151297	7	1.9866	5.1	4.9	4.078	27.7	7.3	0.153111
2	4.319	62	7.3	0.4024	5	3.30827	8.4	8.2	4.319	62.2	4.8	0.390337
3	4.433	78	9.2	0.0165975	10	0.50113	1.3	1.2	4.433	73.3	10.2	0.0278526
4	4.752	123	14.6	0.0091715	5	0.0754365	0.2	0.2	4.752	122	5	0.0085298
5	4.895	143	16.9	0.0112522	7	0.140226	0.4	0.3	4.895	140.8	6.8	0.0116193
6	5.058	166	19.7	0.186177	7	2.30116	5.9	5.7	5.058	166.5	7	0.186723
7	5.245	194	23	0.0163474	24	0.270793	0.7	0.7	5.245	188.3	24	0.0063659
8	5.37	216	25.6	0.26671	7	3.1501	8	7.8	5.37	213.7	7.4	0.240901
9	5.478	235	27.8	0.0414626	16	1.13799	2.9	2.8	5.478	231.9	15.7	0.0409996
10	5.603	257	30.5	0.0693039	8	0.884208	2.3	2.2	5.603	256.4	7.6	0.0656606
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.683	271	32.1	0.0603822	7	0.691764	1.8	1.7	5.683	270.8	6.6	0.0595562
12	5.886	307	36.4	0.0175769	4	0.138092	0.4	0.3	5.886	307.8	4.5	0.0174564
13	6.014	330	39.1	0.345674	5	3.22624	8.2	8	6.014	329.8	5.1	0.358685
14	6.108	347	41.1	0.205143	5	1.83496	4.7	4.6	6.108	347	5.1	0.204177
15	6.208	365	43.2	0.217188	5	1.96378	5	4.9	6.208	364.6	5.1	0.215815
16	6.297	381	45.1	0.0275449	3	0.146466	0.4	0.4	6.297	381.4	3	0.0274362
17	6.38	396	46.9	0.0538245	4	0.368362	0.9	0.9	6.38	396.1	3.9	0.0527572
18	6.519	421	49.9	0.0869512	6	0.724089	1.8	1.8	6.519	420.5	6	0.0675553
19	6.636	436	51.7	0.355966	4	2.24059	5.7	5.6	6.636	435.5	3.7	0.343104
20	6.728	446	52.8	0.207145	5	2.2053	5.6	5.5	6.728	443.5	5	0.249169
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.81	455	53.9	0.09573	5	0.829578	2.1	2.1	6.81	456	4.8	0.0973765
22	6.94	471	55.8	0.0141986	3	0.0700385	0.2	0.2	6.94	471.4	2.8	0.0142082
23	7.032	483	57.2	0.0207416	2	0.0859063	0.2	0.2	7.032	483.5	2.2	0.0218109
24	7.124	495	58.6	0.0222837	11	0.568563	1.4	1.4	7.124	502.7	10.9	0.0293472
25	7.224	508	60.2	0.283401	2	1.16628	3	2.9	7.224	507.7	2.5	0.263746
26	7.655	570	67.5	0.015523	3	0.075923	0.2	0.2	7.655	570.4	2.7	0.0160866
27	7.762	586	69.4	0.0738323	6	0.762684	1.9	1.9	7.762	585.4	6	0.0716949
28	7.816	594	70.4	0.340891	3	1.95967	5	4.9	7.816	594	3.4	0.32302
29	7.949	614	72.7	0.40026	9	5.61321	14.3	14	7.949	615.7	8.7	0.362647
30	8.55	740	87.7	0.0406192	12	0.795613	2	2	8.55	738	11.6	0.0385673

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	95	11.3	0.0739029	4	0.464057	6	4.4	4.55	95.1	3.8	0.0686618
2	5.2	185	22	0.075505	5	0.741034	9.6	7.1	5.2	185.9	5.5	0.0764234
3	5.85	296	35.2	0.1166	4	0.824734	10.7	7.9	5.85	295.9	4.1	0.114342
4	6.55	424	50.4	0.122612	7	1.40817	18.3	13.5	6.55	422.9	7.2	0.110799
5	6.85	457	54.3	0.0370719	5	0.289209	3.8	2.8	6.85	457.4	4.9	0.032966
6	7.35	523	62.1	0.0720623	5	0.574137	7.5	5.5	7.35	522.4	4.7	0.0691703
7	8.15	646	76.7	0.0917857	7	1.13218	14.7	10.9	8.15	645.6	7.1	0.0901901
8	8.45	715	84.9	0.0892947	8	1.24436	16.2	11.9	8.45	713.8	8.3	0.0849577
9	8.65	772	91.7	0.0760407	5	0.620137	8.1	5.9	8.65	771.3	4.8	0.0733899
10	9.3	828	98.3	0.025304	11	0.404336	5.2	3.9	9.3	823.8	10.7	0.0212362

PeakCounter: 10

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 122

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 6.000 5.200 9.700 5.850 8.500 6.550 17.500 6.850 2.800 7.350 6.900 8.150 16.700 8.450 17.800 8.650 9.400 9.300 4.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 10 Return to Lane Navigator
4.088 6.100 4.326 8.700 4.464 1.300 4.755 0.300 4.847 0.400 5.065 5.900 5.260 1.300 5.346 5.300 5.461 4.300 5.592 1.200 5.678 1.400 5.902 0.500 6.023 6.900 6.115 4.100 6.201 4.700 6.271 0.400 6.345 1.100 6.472 1.500 6.501 1.200 6.619 6.000 6.702 3.700 6.832 2.500 6.944 0.600 7.053 0.200 7.147 1.300 7.248 3.000 7.643 0.200 7.731 1.400 7.799 3.100 7.915 12.400 8.565 9.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 31 Return to Lane Navigator
4.550 6.900 5.200 12.000 5.850 12.800 6.550 20.800 6.850 3.300 7.350 6.400 8.150 15.800 8.450 13.100 8.650 7.500 9.300 1.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 10 Return to Lane Navigator
4.082 4.700 4.305 8.900 4.403 0.900 4.550 0.700 4.725 0.300 4.865 0.500 5.039 4.200 5.200 2.900 5.349 7.700 5.464 1.800 5.602 1.200 5.674 1.600 5.850 1.700 6.013 8.300 6.115 4.600 6.205 5.300 6.295 0.300 6.385 0.800 6.514 1.400 6.542 1.300 6.677 7.500 6.760 4.700 6.860 1.400 6.985 0.300 7.071 0.100 7.243 3.000 7.786 5.200 7.944 13.300 8.450 0.900 8.555 1.700 8.650 2.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 31 Return to Lane Navigator
4.078 5.100 4.319 8.400 4.433 1.300 4.752 0.200 4.895 0.400 5.058 5.900 5.245 0.700 5.370 8.000 5.478 2.900 5.603 2.300 5.683 1.800 5.886 0.400 6.014 8.200 6.108 4.700 6.208 5.000 6.297 0.400 6.380 0.900 6.519 1.800 6.636 5.700 6.728 5.600 6.810 2.100 6.940 0.200 7.032 0.200 7.124 1.400 7.224 3.000 7.655 0.200 7.762 1.900 7.816 5.000 7.949 14.300 8.550 2.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 30 Return to Lane Navigator
4.550 6.000 5.200 9.600 5.850 10.700 6.550 18.300 6.850 3.800 7.350 7.500 8.150 14.700 8.450 16.200 8.650 8.100 9.300 5.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 10 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Caribbean Red Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Caribbean Red Snapper ---- Go to Display Gel Lane Navigator.