Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Caribbean Red Snapper  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Lutjanus purpureus
FDA Seafood List Common Name: Caribbean Red Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19940825gel02
Unique Web Code: csh01Srr
Tissue Aliquot Code: lutjpurp H94-002 #07p Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55809.60.075556260.6881048.66.94.5580.45.60.0696804
25.218221.90.12386640.88721411.28.95.2181.34.10.120682
35.8528534.30.16417441.2555415.812.65.85284.94.50.159141
46.5541950.50.10328471.3227816.613.26.55418.37.40.101402
57.3552062.70.041486460.4226975.34.27.35520.86.10.0388152
68.15647780.051308790.7830539.87.88.15646.98.70.0505461
78.4571285.80.080236260.88701911.18.98.45712.76.20.0808939
88.6575891.30.058128540.4409045.54.48.65757.74.50.0555521
99.380096.40.14179151.269251612.79.3799.35.30.136101
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.123161.90.016619960.1789440.70.54.12316.96.40.01566
24.357516.10.21266662.160338.75.44.35750.95.80.21136
34.7310712.90.009256350.0863160.30.24.73106.75.10.0094618
44.8712815.40.014956160.1487460.60.44.87127.360.0140961
55.04315418.60.20628582.9115711.77.35.0431558.50.193715
65.38120724.90.17049441.074174.32.75.381206.73.70.163608
75.725931.20.13939381.709816.94.35.7258.27.50.128492
86.058324390.56450633.2042412.98.16.058324.13.20.556542
96.14340410.39418742.5133210.16.36.14339.93.70.379299
106.227357430.26055252.209828.95.66.227355.54.80.258859
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.30437244.80.034329840.25177410.66.304371.94.10.0349785
126.38138746.60.059274430.2976781.20.76.381386.72.80.0604779
136.483407490.076101950.6124082.51.56.483405.35.40.063921
146.51441349.80.10831330.5577332.21.46.514413.23.50.0909284
156.58242451.10.026707420.1055040.40.36.582423.72.10.0277225
166.66843552.40.5391933.1554212.77.96.668435.13.30.536352
176.73144353.40.39700221.564146.33.96.731442.92.30.385643
186.88946355.80.053231360.5344082.11.36.889462.26.20.0485598
197.00747857.60.036278740.25158710.67.007477.240.0352408
207.09448958.90.017378120.07274450.30.27.094489.52.30.0175424
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.299515620.19632341.26035.13.27.299514.53.80.185596
PeakCounter: 21
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55809.60.086486650.7163688.96.94.5580.54.80.0834284
25.217320.80.11704940.82887410.385.2173.340.115701
35.85282340.1585251.2450515.5125.85282.34.50.155301
46.5542150.70.11492861.2109615.111.76.55421.36.20.109438
57.35523630.040836660.4229715.34.17.35523.46.10.0388121
68.1564477.60.065422380.87257210.98.48.15643.17.70.0635262
78.4571085.50.082097460.90664211.38.88.45709.96.30.081076
88.6575691.10.062094340.4608515.84.58.65755.74.40.0592321
99.380096.40.14396461.3444316.8139.3799.75.60.136482
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.34576.90.073369460.7439953.52.84.3457.65.80.0722509
24.558910.70.048157440.3613621.71.44.5589.24.40.0463214
34.71411413.70.006656370.07324420.30.34.714113.47.10.0057983
44.83213215.90.009170670.08943290.40.34.832131.86.80.0074285
55.0115919.10.13102271.302946.24.95.011606.70.110449
65.218822.60.0407671181.315926.355.2177.317.80.041609
75.33921025.30.057378940.4447432.11.75.339210.14.30.0580811
85.66126131.40.0668780.8759914.23.35.661259.480.0620743
95.85291350.067832940.4834392.31.85.85290.84.10.0672598
106.04532739.40.39599142.5850712.39.86.045326.33.70.391164
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.13134341.30.25802541.982919.47.56.131343.34.50.251152
126.22336043.30.16898251.501547.15.76.223359.34.90.173588
136.30537545.10.025345430.1488120.70.66.305375.33.30.0252525
146.39139147.10.018415430.1070570.50.46.391389.62.60.0232149
156.48940949.20.042297530.2336611.10.96.489408.33.30.0400831
166.52741650.10.082629940.5637542.72.16.527415.940.079959
176.67343652.50.47987332.9316513.911.16.673436.43.40.489825
186.73644453.40.30857531.416546.75.46.736443.72.60.306926
196.89246455.80.029872970.42824321.66.8924617.30.0332556
206.99447757.40.014477230.06863860.30.36.994478.43.30.0115801
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.096490590.012258820.05542680.30.27.096489.62.40.0130406
227.28551461.90.11844730.63355832.47.285513.830.11747
237.95761774.20.068843450.7084283.42.77.957616.65.30.0749509
248.1564777.90.022643680.3059461.51.28.15648.28.10.0212644
258.4571385.80.062301580.7962533.838.45713.27.50.0598504
268.6575390.60.036433250.3139521.51.28.65753.15.20.0341862
279.379996.10.065903150.5774332.72.29.3798.850.0648814
PeakCounter: 27
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.086161.90.01209660.1153530.40.34.086175.60.011613
24.315516.10.21575162.286388.66.44.3155160.213916
34.71311213.50.010219750.0923620.30.34.713111.55.10.0101199
44.851133160.014943360.150590.60.44.851132.860.0140541
55.066166200.14376271.296384.93.65.066165.96.70.109052
65.18418422.10.111194132.8132910.67.95.184180.613.20.119854
75.41722126.60.16862741.086534.135.417221.43.80.159538
85.70626732.10.16632782.055287.85.85.706265.27.70.149706
96.07133139.80.58063533.0026711.38.46.071331.530.560608
106.15234641.60.42254942.578439.77.26.1523463.60.408739
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.23336143.40.28327742.284598.66.46.233360.24.50.286798
126.303374450.043765430.25615710.76.303374.73.30.043925
136.38438946.80.065725330.3247261.20.96.384389.12.70.0669398
146.482407490.074713750.6642762.51.96.482407.74.80.078254
156.51941449.80.11357220.3827521.41.16.519414.520.108012
166.584424510.012298610.03288920.10.16.584423.11.40.0134161
176.66243452.20.55200433.2006212.196.662433.83.20.559121
186.71744153.10.39137121.65276.24.66.717441.32.30.399439
196.86646055.40.040144540.3167621.20.96.866459.84.20.0430053
206.976474570.055975840.442841.71.26.9764744.20.0590867
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.07848758.60.018970220.07039880.30.27.078486.21.80.0216216
227.26651161.50.25340431.362135.13.87.266510.73.20.243611
PeakCounter: 22
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.558510.20.099945660.9280729.37.44.5584.55.60.0937723
25.218522.30.13637240.9880929.97.95.2185.24.10.134581
35.8528934.80.18678451.4761314.811.85.85288.74.60.181386
46.5541950.40.1463261.5516315.612.46.55418.56.30.139427
57.3552162.70.053856850.4930514.93.97.35520.95.30.0524213
68.1564277.30.073166670.9422479.47.58.156417.50.0711415
78.4570584.80.10783371.2318512.49.88.45705.66.60.105976
88.6575390.60.066097840.5039685.148.657534.40.0653288
99.3798960.18876661.8567218.614.89.3797.45.70.183436
PeakCounter: 9
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 97
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 21 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 27 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 22 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 9 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln