Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Coho Salmon  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Oncorhynchus kisutch
FDA Seafood List Common Name: Coho Salmon
FDA Seafood List Market Name: Salmon, Coho (Silver, Medium Red)
Unique IEF Gel Code: 19940906gel02
Unique Web Code: coh01Srr
Tissue Aliquot Code: oncokisu H94-002 #01m Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.555770.042298340.2737843.61.94.5557.73.90.0399716
25.215218.80.040211740.2873773.825.2151.640.0400814
35.8526132.30.06257150.5199796.93.65.85260.64.70.0622233
46.5538647.70.049505750.4228675.636.55385.95.30.0447394
57.3549060.60.11767371.3266517.79.37.35491.17.20.103352
68.1561676.10.041032480.5767627.748.15613.67.60.0428349
78.4568084.10.081678981.12421157.98.45679.88.30.0765756
88.6572689.70.0618153241.8301124.412.88.65737.5240.0430229
99.377495.70.095895391.1470815.38.19.3770.88.90.0727668
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.6687290.026024740.169610.40.44.6687240.024168
25.02112415.50.0520404110.9201662.32.25.021121.311.10.0466745
35.43318823.40.058641630.2885650.70.75.433187.42.70.0602041
45.64522227.70.087409550.77015321.85.645220.45.30.0818376
56.15531238.90.20826630.9433752.42.36.155312.32.60.202476
66.21932440.40.16301840.9636562.42.36.219324.33.60.151412
76.288337420.070338150.6657451.71.66.288336.45.50.0684629
86.38435544.30.52712732.602666.66.26.384355.42.80.529019
96.49137546.80.2724631.646914.23.96.491374.63.50.26733
106.58239048.60.19537251.632384.13.96.582389.15.20.176002
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.70140550.50.28251341.799794.64.36.701405.63.50.288017
126.76541351.50.3961331.9758654.76.765412.72.80.396425
136.86425530.063071630.3003260.80.76.864252.70.0624589
147.19546758.20.22456531.080252.72.67.195467.42.70.226899
157.366489610.17849451.40633.63.47.366488.74.60.172115
167.43950062.30.30809531.5796843.87.439499.72.90.302538
177.5351464.10.34316842.529236.46.17.53514.34.30.333672
187.7654968.50.21144651.339053.43.27.76547.65.20.144366
197.79355469.10.27364962.485756.35.97.793554.860.233087
208.20262077.30.088377192.865087.36.98.20262619.10.0847168
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.73872990.90.19626154.722081211.38.738729.414.70.181766
228.94174492.80.24340741.113932.82.78.941743.64.20.14827
239.10475694.30.22348793.425238.78.29.104757.48.90.216578
249.22676595.40.25220462.120585.45.19.226768.26.40.186907
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55546.80.026496860.2534616.14.44.555460.0239058
25.215118.90.050940140.33335785.75.2150.83.90.0480529
35.8525131.50.086103830.51731212.48.95.85251.53.50.0842958
46.5538848.60.039995590.55350113.39.56.55387.58.50.0366182
57.3548560.80.025623360.2533646.14.47.35484.55.90.0244002
68.1560375.60.040056170.49627611.98.58.15601.67.10.0395804
78.4567284.20.064013850.58172113.9108.45671.25.40.0613278
88.6572190.40.039304740.2542216.14.48.65720.53.90.0367493
99.376996.40.092903960.93006522.3169.3768.56.30.0838949
PeakCounter: 9
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55577.20.0189750.1443340.50.54.5556.94.50.017928
24.9711914.90.0261466100.4319861.61.54.97116.710.40.0234016
35.215319.20.023876150.1800750.70.65.2152.74.70.0217088
45.35517922.50.032489850.26514810.95.355179.84.90.0302361
55.522207260.03845190.595862.32.15.522205.290.0374214
65.8526232.90.04129650.3765291.41.35.85260.35.40.0396516
76.07830338.10.085339340.6170732.32.26.078302.64.50.0779265
86.17832140.30.071732160.6749122.62.46.178319.85.80.0654143
96.26133642.20.033126870.4065031.51.46.261335.66.90.0332375
106.37835744.80.30884831.772496.76.36.378356.73.40.296198
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.472374470.17645741.163914.44.16.472374.13.80.172561
126.5538848.70.13519551.0555343.76.55386.94.70.127204
136.6540050.30.18265931.183014.54.26.65401.13.30.20269
146.70840751.10.27679431.290454.94.66.708407.42.60.28021
156.80841952.60.035864930.1825010.70.66.808419.32.90.0355818
167.167462580.13319930.646782.42.37.167462.12.80.130743
177.3548460.80.15004851.205084.64.37.35484.44.70.145947
187.41549462.10.21810731.021783.93.67.415493.82.70.212097
197.49250663.60.24488951.988467.57.17.4925074.70.239604
207.70553967.70.19892872.1269687.57.705541.36.70.179815
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.1560876.40.0547101131.284264.94.68.1561313.40.0538793
228.4567684.90.023649750.2082240.80.78.45674.45.30.0219733
238.6572390.80.138406102.287388.78.18.65722.610.20.12706
248.87873992.80.16899661.50695.75.38.878738.95.80.146364
259.09175494.70.16009561.012363.83.69.091750.85.60.101746
269.21976395.90.17380592.8103910.6109.219762.18.90.179063
PeakCounter: 26
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.656729.10.021115550.1651920.50.54.65672.24.70.0199782
24.98812215.30.0478918100.7166812.124.988120.59.60.0419704
35.39918723.50.062259440.4343521.31.25.399186.94.10.059892
45.567215270.094968171.214333.63.45.567213.87.40.0926719
56.09230638.50.21173531.093113.33.16.09230630.20609
66.1932440.80.13282340.8997742.72.56.19323.54.40.11609
76.27333942.60.057951370.67475421.96.273338.86.90.0551117
86.38335945.20.5229632.592577.77.36.383359.42.90.51133
96.48137747.40.30706741.880125.65.36.481376.63.60.291773
106.55439049.10.20673341.439694.346.554389.64.20.191699
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.66140350.70.32421731.626064.84.66.661403.52.80.324595
126.71841051.60.42736432.095126.25.96.718409.72.70.43055
136.808421530.063580630.2958290.90.86.808421.32.60.0644697
147.15346358.20.25337631.10543.33.17.153463.22.50.24514
157.34248661.10.16925251.351643.87.342485.94.70.162798
167.4149662.40.35318231.734475.24.97.41495.92.80.350206
177.51651264.40.24441273.0135898.47.516513.46.80.248917
187.72254368.30.17385930.7869492.32.27.722542.92.50.174176
197.85556370.80.109376112.001265.67.855561.411.10.101497
208.17612770.0889023172.806428.47.98.17617.917.40.0912009
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.65772491.10.046882570.5494831.61.58.657722.36.60.0466577
228.90774293.30.1432951.125073.43.28.907741.44.80.13363
239.10175695.10.1694950.9476732.82.79.1017535.10.105405
249.21276496.10.20844582.983758.98.49.212763.47.90.211923
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55496.20.042778450.3454036.85.14.5549.14.80.0404085
25.214918.90.080856830.4884129.77.35.21493.50.079078
35.8525632.50.12184340.73815114.6115.85255.63.60.116578
46.5538548.90.061442460.62321912.39.36.5538560.0590743
57.3548461.40.039534330.2284564.53.47.35484.43.40.0379932
68.1560176.30.040110380.55912711.18.38.15601.48.20.0385089
78.4566884.80.063508470.78308115.511.68.45667.37.20.0613511
88.6571891.10.046110440.3098216.14.68.65717.440.0442402
99.376697.20.10250360.9736219.314.59.3765.15.70.0970683
PeakCounter: 9
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.45 label for gel schematic
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pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
white spacer for gel schematic
pI 8.45 label for gel schematic
white spacer for gel schematic
pI 8.65 label for gel schematic
white spacer for gel schematic
pI 9.30 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 101
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 9 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 26 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 9 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln