RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Coho Salmon ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Oncorhynchus kisutch
FDA Seafood List Common Name: Coho Salmon
FDA Seafood List Market Name: Salmon, Coho (Silver, Medium Red)
Unique IEF Gel Code: 19940830gel04
Unique Web Code: cog02Srr
Tissue Aliquot Code: oncokisu H94-003 #04a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 11
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 13
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	47	4.2	0.182731	17	5.39062	18.1	14.6	3.5	51.4	17.3	0.1754
2	4.55	185	16.4	0.333559	4	2.23928	7.5	6.1	4.55	184.8	4.3	0.290733
3	5.2	262	23.2	0.448729	4	3.10814	10.4	8.4	5.2	262	4	0.435366
4	5.85	410	36.3	0.595228	3	3.13629	10.5	8.5	5.85	409.9	2.9	0.606465
5	6.55	577	51.1	0.394773	4	2.49673	8.4	6.8	6.55	576.3	3.8	0.3715
6	6.85	614	54.4	0.122686	6	1.16415	3.9	3.1	6.85	614.2	6.3	0.105054
7	7.35	708	62.7	0.39132	4	3.00079	10.1	8.1	7.35	707.5	4.5	0.377724
8	8.15	875	77.5	0.125783	9	1.95103	6.5	5.3	8.15	873.3	9.1	0.12062
9	8.45	937	83	0.164015	7	1.86823	6.3	5.1	8.45	937	6.5	0.162038
10	8.65	978	86.6	0.114565	6	1.21955	4.1	3.3	8.65	978.3	6.2	0.11098
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1101	97.5	0.541617	5	4.24252	14.2	11.5	9.3	1100.5	4.9	0.492048

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 12
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 14
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.959	109	9.6	0.0958464	4	0.677578	1.4	1.2	3.959	108.4	4.1	0.0933003
2	4.67	202	17.9	0.133932	4	0.872042	1.8	1.6	4.67	201.5	4	0.123394
3	4.869	226	20	0.0439401	3	0.273664	0.6	0.5	4.869	224.7	3	0.0517955
4	5.026	245	21.7	0.120705	3	0.588119	1.2	1.1	5.026	244.2	2.9	0.113921
5	5.101	254	22.5	0.224532	6	2.22033	4.6	4.1	5.101	254.9	6.3	0.199026
6	5.311	291	25.7	0.0468	6	0.424004	0.9	0.8	5.311	292	6	0.0396779
7	5.386	308	27.2	0.0866434	7	0.994623	2	1.8	5.386	305.9	7	0.0801707
8	5.483	330	29.2	0.322183	3	1.73171	3.6	3.2	5.483	330	3	0.320935
9	5.518	338	29.9	0.375694	3	2.15909	4.4	4	5.518	338.2	3.4	0.356006
10	5.642	366	32.4	0.225762	3	1.29045	2.7	2.4	5.642	366.2	3.2	0.229787
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.695	378	33.4	0.0349478	2	0.303348	0.6	0.6	5.695	376.2	1.7	0.101644
12	6.002	449	39.7	0.591546	2	2.49441	5.1	4.6	6.002	448.9	2.4	0.578165
13	6.095	471	41.6	0.285347	3	1.29871	2.7	2.4	6.095	471.3	2.9	0.252821
14	6.343	530	46.9	0.743531	3	3.69749	7.6	6.8	6.343	530.3	2.8	0.745617
15	6.44	553	48.9	0.436581	2	1.85543	3.8	3.4	6.44	552.7	2.4	0.436089
16	6.483	563	49.8	0.12414	2	0.409293	0.8	0.8	6.483	562.8	1.9	0.12385
17	6.516	571	50.5	0.10067	3	0.541295	1.1	1	6.516	572	2.8	0.110086
18	6.558	580	51.3	0.268808	3	1.20511	2.5	2.2	6.558	579.5	2.5	0.271816
19	6.615	587	51.9	0.395713	3	2.38702	4.9	4.4	6.615	587.5	3.3	0.404159
20	6.745	603	53.3	0.254675	2	0.861372	1.8	1.6	6.745	602.9	1.9	0.254372
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.139	670	59.2	0.167375	2	0.964862	2	1.8	7.139	668.7	1.9	0.29011
22	7.321	704	62.2	0.155842	6	1.46516	3	2.7	7.321	704.3	5.6	0.147986
23	7.396	719	63.6	0.233331	2	0.970725	2	1.8	7.396	719.4	2.3	0.233217
24	7.544	750	66.3	0.50626	5	4.25288	8.8	7.8	7.544	749.9	4.9	0.494116
25	7.697	782	69.1	0.0945577	3	0.568347	1.2	1	7.697	781.3	3.5	0.0919453
26	7.812	806	71.3	0.478976	7	5.68185	11.7	10.4	7.812	806.1	6.8	0.472274
27	8.479	945	83.6	0.063193	9	0.993649	2	1.8	8.479	943.1	8.9	0.0632658
28	8.671	984	87	0.234335	16	6.17291	12.7	11.3	8.671	984.9	15.7	0.221239
29	8.809	1010	89.3	0.0304039	6	0.219275	0.5	0.4	8.809	1012.9	6.2	0.0199464
30	8.937	1034	91.4	0.0495299	5	0.47817	1	0.9	8.937	1032.5	5.3	0.050712
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	9.027	1051	92.9	0.0739916	4	0.51777	1.1	1	9.027	1050.9	3.9	0.0757021

PeakCounter: 31

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 13
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 15
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	47	4.2	0.186923	12	3.82238	12.6	10.2	3.5	49.5	12.1	0.177726
2	4.55	188	16.6	0.305727	5	2.41483	8	6.5	4.55	188.2	4.9	0.278601
3	5.2	268	23.7	0.404231	5	3.4626	11.4	9.3	5.2	268.2	4.9	0.400506
4	5.69	378	33.5	0.0488655	4	0.331083	1.1	0.9	5.69	377.7	3.8	0.0486258
5	5.85	414	36.6	0.547733	3	3.12768	10.3	8.4	5.85	414.3	3.2	0.549315
6	6.55	579	51.2	0.428557	4	2.54924	8.4	6.8	6.55	579	3.5	0.409184
7	6.85	616	54.5	0.128831	7	1.29336	4.3	3.5	6.85	616.2	6.5	0.112225
8	7.35	709	62.7	0.442478	4	3.41065	11.2	9.1	7.35	708.5	4.5	0.428002
9	8.15	876	77.5	0.120731	9	1.81661	6	4.9	8.15	875.5	8.7	0.117583
10	8.45	939	83.1	0.190209	6	2.02817	6.7	5.4	8.45	939.1	6.1	0.188342
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	8.65	980	86.7	0.134936	6	1.35062	4.5	3.6	8.65	979.9	5.9	0.128076
12	9.3	1102	97.5	0.632629	5	4.7184	15.6	12.6	9.3	1102	4.7	0.56202

PeakCounter: 12

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 14
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 16
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	44	3.9	0.234904	14	4.83911	5.7	5.4	3.5	46	13.9	0.197112
2	3.766	81	7.2	0.050537	17	1.6148	1.9	1.8	3.766	72.4	17.3	0.0525344
3	4.55	190	16.8	0.285374	4	1.29916	1.5	1.4	4.55	189	3.7	0.199347
4	4.609	197	17.4	0.168215	7	1.88898	2.2	2.1	4.609	195.1	6.6	0.16205
5	4.786	218	19.3	0.041896	3	0.242893	0.3	0.3	4.786	218.5	3.1	0.0435057
6	4.964	239	21.2	0.136202	3	0.59016	0.7	0.7	4.964	238	2.6	0.127258
7	5.04	248	21.9	0.200354	8	2.35618	2.8	2.6	5.04	249.9	7.7	0.171863
8	5.2	267	23.6	0.372532	5	2.83616	3.4	3.2	5.2	267	4.5	0.354525
9	5.365	304	26.9	0.0740544	9	1.0238	1.2	1.1	5.365	302.1	9.1	0.0633724
10	5.472	328	29	0.263021	3	1.52971	1.8	1.7	5.472	328.1	3.3	0.265361
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.507	336	29.7	0.349335	4	2.26723	2.7	2.5	5.507	336.6	3.8	0.336924
12	5.583	353	31.2	0.135726	6	1.15343	1.4	1.3	5.583	352.1	5.6	0.116539
13	5.645	367	32.5	0.22016	3	1.21547	1.4	1.4	5.645	366.7	3.1	0.224541
14	5.685	376	33.3	0.0982895	2	0.404895	0.5	0.4	5.685	376.8	2.3	0.100048
15	5.85	413	36.5	0.538806	3	2.82131	3.3	3.1	5.85	413.2	2.9	0.541928
16	6.008	450	39.8	0.550892	3	2.53693	3	2.8	6.008	449.9	2.6	0.540644
17	6.106	473	41.9	0.271322	3	1.44643	1.7	1.6	6.106	472.8	3.2	0.256123
18	6.366	534	47.3	0.699175	3	3.68427	4.4	4.1	6.366	533.9	3	0.699856
19	6.465	557	49.3	0.468924	2	1.93267	2.3	2.1	6.465	556.8	2.4	0.462498
20	6.512	568	50.3	0.10552	2	0.349605	0.4	0.4	6.512	568.2	1.8	0.106688
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.55	577	51.1	0.28522	3	1.31044	1.6	1.5	6.55	576.9	2.6	0.284311
22	6.625	585	51.8	0.347183	2	1.35396	1.6	1.5	6.625	584.8	2.2	0.345899
23	6.7	593	52.5	0.473737	3	2.77899	3.3	3.1	6.7	593.1	3.2	0.484278
24	6.85	609	53.9	0.250109	2	0.994724	1.2	1.1	6.85	608.6	2.2	0.254424
25	7.185	674	59.6	0.310164	2	1.21633	1.4	1.4	7.185	674.7	2	0.34813
26	7.35	706	62.5	0.453149	4	3.35211	4	3.7	7.35	705.7	4.4	0.432535
27	7.446	725	64.2	0.421972	3	2.38218	2.8	2.6	7.446	724.9	3.5	0.389044
28	7.735	782	69.2	0.250974	9	3.75663	4.4	4.2	7.735	780.7	8.9	0.238855
29	7.775	790	69.9	0.132963	4	0.565008	0.7	0.6	7.775	787.4	3.8	0.0834373
30	7.993	833	73.7	0.495892	4	3.77197	4.5	4.2	7.993	832.8	4.3	0.498804
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	8.15	864	76.5	0.0893444	16	3.205	3.8	3.6	8.15	873.3	16.1	0.112427
32	8.45	936	82.8	0.202954	6	1.63218	1.9	1.8	8.45	934.3	6.4	0.143398
33	8.509	951	84.2	0.157519	17	4.43326	5.2	4.9	8.509	951.3	16.9	0.148166
34	8.65	987	87.3	0.36053	18	11.1444	13.2	12.4	8.65	986.9	18	0.349315
35	8.797	1013	89.6	0.106809	7	0.755366	0.9	0.8	8.797	1011.2	6.7	0.0632427
36	8.916	1034	91.5	0.0755013	5	0.554067	0.7	0.6	8.916	1033.9	4.7	0.0664725
37	9.023	1053	93.2	0.022406	3	0.207636	0.2	0.2	9.023	1050.6	3.3	0.0358636
38	9.3	1102	97.5	0.618859	5	5.09624	6	5.7	9.3	1101	5.4	0.536632

PeakCounter: 38

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 15
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 17
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.797	83	7.3	0.0346897	7	0.474951	1	0.9	3.797	83.8	6.9	0.0388271
2	4.658	200	17.7	0.0975325	5	0.765649	1.6	1.5	4.658	199.8	4.8	0.0900174
3	4.85	223	19.7	0.0348276	3	0.221283	0.5	0.4	4.85	221.9	3.3	0.0380901
4	5.017	243	21.5	0.0914769	2	0.307331	0.7	0.6	5.017	241.4	2.5	0.0693989
5	5.092	252	22.3	0.214444	8	2.62757	5.6	5	5.092	252.7	7.6	0.195019
6	5.393	308	27.2	0.0556201	7	0.708958	1.5	1.3	5.393	306.3	7.4	0.0542225
7	5.491	330	29.2	0.256748	3	1.58878	3.4	3	5.491	330.3	3.5	0.259382
8	5.527	338	29.9	0.366765	4	2.3202	4.9	4.4	5.527	338.5	3.7	0.356803
9	5.599	354	31.3	0.139405	6	1.26804	2.7	2.4	5.599	353.3	6	0.119681
10	5.662	368	32.5	0.245016	3	1.32302	2.8	2.5	5.662	368.3	3	0.247499
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.707	378	33.4	0.0704869	2	0.270487	0.6	0.5	5.707	378.8	1.9	0.0796293
12	6.023	452	40	0.546051	3	2.36741	5	4.5	6.023	452.2	2.6	0.522418
13	6.114	474	41.9	0.315108	3	1.47048	3.1	2.8	6.114	473.9	2.9	0.286825
14	6.354	532	47	0.694516	3	3.87394	8.2	7.3	6.354	532.2	3.2	0.691505
15	6.445	554	49	0.487699	2	2.02011	4.3	3.8	6.445	553.7	2.4	0.479884
16	6.49	565	50	0.111547	2	0.364569	0.8	0.7	6.49	565.1	1.8	0.11197
17	6.523	573	50.7	0.0824876	3	0.53454	1.1	1	6.523	574.6	2.8	0.107955
18	6.572	582	51.5	0.302676	2	1.32776	2.8	2.5	6.572	581.4	2.5	0.305473
19	6.633	589	52.1	0.427811	3	2.37484	5	4.5	6.633	589.5	3.1	0.432865
20	6.772	605	53.5	0.226571	2	0.781721	1.7	1.5	6.772	605.3	1.9	0.231901
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.145	670	59.2	0.299134	2	0.912897	1.9	1.7	7.145	670	1.8	0.291379
22	7.4	719	63.6	0.2429	4	1.57852	3.4	3	7.4	717.3	4	0.223113
23	7.633	765	67.6	0.311631	6	3.16125	6.7	6	7.633	765	5.8	0.306398
24	7.698	778	68.8	0.0725366	4	0.440707	0.9	0.8	7.698	778.2	3.7	0.0680991
25	7.915	821	72.6	0.552019	4	3.68962	7.8	7	7.915	820.5	3.7	0.55657
26	8.467	940	83.1	0.0693531	9	1.07993	2.3	2	8.467	938.7	8.7	0.0700302
27	8.624	976	86.3	0.306789	14	6.89596	14.7	13.1	8.624	976.4	13.9	0.278928
28	8.781	1006	88.9	0.0825604	7	0.723371	1.5	1.4	8.781	1010.1	6.9	0.0587675
29	8.889	1026	90.7	0.0975411	8	1.36574	2.9	2.6	8.889	1024.5	8.3	0.0930204
30	9.004	1047	92.6	0.0389468	3	0.195644	0.4	0.4	9.004	1045.9	2.8	0.0400051

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 16
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 18
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	40	3.5	0.269035	12	4.78842	13.8	10.9	3.5	41.2	11.8	0.229575
2	4.55	184	16.3	0.437935	4	2.90652	8.4	6.6	4.55	184.5	4.1	0.397418
3	5.2	263	23.3	0.580567	4	4.00523	11.5	9.1	5.2	262.4	4	0.566383
4	5.85	407	36	0.745022	2	3.35093	9.6	7.6	5.85	407.4	2.5	0.758901
5	6.55	582	51.5	0.509916	4	3.42078	9.8	7.8	6.55	581.9	4	0.488455
6	6.85	619	54.7	0.146444	6	1.25443	3.6	2.8	6.85	619.1	5.5	0.127836
7	7.35	712	63	0.506983	5	4.0384	11.6	9.2	7.35	711.8	4.6	0.49939
8	8.15	871	77	0.113385	10	1.8612	5.4	4.2	8.15	870.8	9.7	0.108175
9	8.45	936	82.8	0.179173	7	2.02247	5.8	4.6	8.45	936	6.6	0.172622
10	8.65	977	86.4	0.150446	6	1.49461	4.3	3.4	8.65	976	5.8	0.146138
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1101	97.3	0.580204	6	5.61229	16.1	12.7	9.3	1099.9	5.9	0.537102

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 133

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 18.100 4.550 7.500 5.200 10.400 5.850 10.500 6.550 8.400 6.850 3.900 7.350 10.100 8.150 6.500 8.450 6.300 8.650 4.100 9.300 14.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 11 LANE TYPE: pI Markers ACTUAL GEL LANE: 13 PeakCount: 11 Return to Lane Navigator
3.959 1.400 4.670 1.800 4.869 0.600 5.026 1.200 5.101 4.600 5.311 0.900 5.386 2.000 5.483 3.600 5.518 4.400 5.642 2.700 5.695 0.600 6.002 5.100 6.095 2.700 6.343 7.600 6.440 3.800 6.483 0.800 6.516 1.100 6.558 2.500 6.615 4.900 6.745 1.800 7.139 2.000 7.321 3.000 7.396 2.000 7.544 8.800 7.697 1.200 7.812 11.700 8.479 2.000 8.671 12.700 8.809 0.500 8.937 1.000 9.027 1.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 12 LANE TYPE: Specimen ACTUAL GEL LANE: 14 PeakCount: 31 Return to Lane Navigator
3.500 12.600 4.550 8.000 5.200 11.400 5.690 1.100 5.850 10.300 6.550 8.400 6.850 4.300 7.350 11.200 8.150 6.000 8.450 6.700 8.650 4.500 9.300 15.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 13 LANE TYPE: pI Markers ACTUAL GEL LANE: 15 PeakCount: 12 Return to Lane Navigator
3.500 5.700 3.766 1.900 4.550 1.500 4.609 2.200 4.786 0.300 4.964 0.700 5.040 2.800 5.200 3.400 5.365 1.200 5.472 1.800 5.507 2.700 5.583 1.400 5.645 1.400 5.685 0.500 5.850 3.300 6.008 3.000 6.106 1.700 6.366 4.400 6.465 2.300 6.512 0.400 6.550 1.600 6.625 1.600 6.700 3.300 6.850 1.200 7.185 1.400 7.350 4.000 7.446 2.800 7.735 4.400 7.775 0.700 7.993 4.500 8.150 3.800 8.450 1.900 8.509 5.200 8.650 13.200 8.797 0.900 8.916 0.700 9.023 0.200 9.300 6.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 14 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 16 PeakCount: 38 Return to Lane Navigator
3.797 1.000 4.658 1.600 4.850 0.500 5.017 0.700 5.092 5.600 5.393 1.500 5.491 3.400 5.527 4.900 5.599 2.700 5.662 2.800 5.707 0.600 6.023 5.000 6.114 3.100 6.354 8.200 6.445 4.300 6.490 0.800 6.523 1.100 6.572 2.800 6.633 5.000 6.772 1.700 7.145 1.900 7.400 3.400 7.633 6.700 7.698 0.900 7.915 7.800 8.467 2.300 8.624 14.700 8.781 1.500 8.889 2.900 9.004 0.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 15 LANE TYPE: Specimen ACTUAL GEL LANE: 17 PeakCount: 30 Return to Lane Navigator
3.500 13.800 4.550 8.400 5.200 11.500 5.850 9.600 6.550 9.800 6.850 3.600 7.350 11.600 8.150 5.400 8.450 5.800 8.650 4.300 9.300 16.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 16 LANE TYPE: pI Markers ACTUAL GEL LANE: 18 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Coho Salmon ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Coho Salmon ---- Go to Display Gel Lane Navigator.