RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Canary Rockfish ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Sebastes pinniger
FDA Seafood List Common Name: Canary Rockfish
FDA Seafood List Market Name: Rockfish
Unique IEF Gel Code: 19940908gel05
Unique Web Code: cah01Srr
Tissue Aliquot Code: sebapinn I94-002 #09a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	75	9.2	0.0676467	6	0.676184	11.3	8.8	4.55	75.2	6	0.0639122
2	5.2	172	21	0.074614	5	0.670138	11.2	8.7	5.2	171.8	5.1	0.0741046
3	5.85	278	34	0.119575	4	0.775766	13	10.1	5.85	278.4	3.7	0.11834
4	6.55	415	50.7	0.0396276	7	0.472173	7.9	6.2	6.55	413.5	7.2	0.0369045
5	7.35	512	62.6	0.0396134	5	0.336947	5.6	4.4	7.35	511.7	5.1	0.0374431
6	8.15	634	77.5	0.0426134	9	0.709322	11.9	9.2	8.15	634	9.5	0.0423109
7	8.45	699	85.5	0.0803841	6	0.875815	14.7	11.4	8.45	698.2	6.3	0.0782778
8	8.65	746	91.2	0.0525816	4	0.395235	6.6	5.2	8.65	745.2	4.4	0.0511529
9	9.3	795	97.2	0.0970181	7	1.06406	17.8	13.9	9.3	793.9	6.7	0.0896914

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.139	18	2.2	0.0721237	5	0.395264	1.9	1.1	4.139	18.4	4.7	0.0474127
2	4.228	31	3.8	0.130609	10	2.30109	11.1	6.5	4.228	31	10.4	0.125331
3	4.671	95	11.6	0.415701	5	3.94023	19	11.2	4.671	95.6	5.5	0.406795
4	5.936	295	36	0.544441	3	3.09123	14.9	8.8	5.936	295.4	3.2	0.543176
5	5.982	304	37.1	0.38129	3	2.04607	9.9	5.8	5.982	304	3.1	0.37511
6	6.034	314	38.3	0.221724	5	1.71339	8.3	4.9	6.034	314.5	4.7	0.205787
7	6.153	337	41.1	0.0724441	2	0.186338	0.9	0.5	6.153	336.9	2	0.0521653
8	6.2	346	42.2	0.051348	13	1.17775	5.7	3.3	6.2	349.2	13	0.0512161
9	6.226	351	42.8	0.384341	2	1.17905	5.7	3.3	6.226	350.6	1.9	0.35017
10	6.34	373	45.5	0.113119	9	1.47726	7.1	4.2	6.34	371.5	8.5	0.0974995
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.418	388	47.3	0.0161344	1	0	0	0	6.418	382	1	0
12	6.459	396	48.3	0.0615235	3	0.245294	1.2	0.7	6.459	396.2	2.5	0.0551282
13	6.496	403	49.1	0.0687545	5	0.516988	2.5	1.5	6.496	402.8	4.5	0.0644055
14	6.627	423	51.6	0.0160544	8	0.194759	0.9	0.6	6.627	424.2	7.8	0.0141234
15	7.389	518	63.2	0.0756068	8	0.870955	4.2	2.5	7.389	517.6	7.5	0.0654429
16	7.526	539	65.7	0.0319264	9	0.453265	2.2	1.3	7.526	535.6	8.9	0.0287131
17	7.65	558	68	0.174449	3	0.936559	4.5	2.7	7.65	557.8	3	0.175074

PeakCounter: 17

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	79	9.6	0.0604221	4	0.44287	7.4	5.8	4.55	78.9	4.3	0.0587481
2	5.2	170	20.7	0.0894944	4	0.593601	10	7.8	5.2	170	3.8	0.0871372
3	5.85	278	33.9	0.103722	4	0.690613	11.6	9	5.85	277.6	3.9	0.100015
4	6.55	411	50.1	0.0481146	8	0.661493	11.1	8.6	6.55	412.2	8	0.0467633
5	7.35	511	62.2	0.0451353	6	0.466593	7.8	6.1	7.35	511.6	6.1	0.0434707
6	8.15	634	77.2	0.0625612	7	0.781066	13.1	10.2	8.15	633.9	7.4	0.0592219
7	8.45	697	84.9	0.0839341	7	1.01145	17	13.2	8.45	696	6.8	0.0839198
8	8.65	745	90.7	0.0493277	4	0.375242	6.3	4.9	8.65	745.2	4.4	0.0480872
9	9.3	796	97	0.0852445	7	0.924921	15.6	12.1	9.3	794.7	6.5	0.0797429

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.286	40	4.9	0.0569668	11	1.078	6.9	4.8	4.286	41.3	11.4	0.0533571
2	4.55	79	9.6	0.0217011	4	0.143494	0.9	0.6	4.55	79.1	3.8	0.0213439
3	4.672	97	11.8	0.215207	8	2.6834	17.1	11.9	4.672	98.7	7.6	0.198551
4	5.2	175	21.3	0.0232583	5	0.192543	1.2	0.9	5.2	173.8	4.6	0.0235575
5	5.85	282	34.3	0.0341127	3	0.197806	1.3	0.9	5.85	282	3.3	0.0339084
6	5.926	296	36.1	0.329805	4	2.43138	15.5	10.7	5.926	295.6	4.3	0.318908
7	5.98	306	37.3	0.231945	4	1.7591	11.2	7.8	5.98	305.6	4.5	0.222018
8	6.023	314	38.2	0.121767	4	0.864253	5.5	3.8	6.023	313.8	4.3	0.114164
9	6.153	338	41.2	0.0309509	5	0.239286	1.5	1.1	6.153	337.9	4.6	0.0293268
10	6.196	346	42.1	0.053055	3	0.257868	1.6	1.1	6.196	346.9	2.6	0.0561597
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.229	352	42.9	0.200655	3	0.927811	5.9	4.1	6.229	351.4	2.7	0.194154
12	6.348	374	45.6	0.077774	6	0.778161	5	3.4	6.348	375.1	5.6	0.0778846
13	6.429	389	47.4	0.0214503	3	0.11001	0.7	0.5	6.429	386.8	3.2	0.0195742
14	6.472	397	48.4	0.0591339	7	0.626677	4	2.8	6.472	398.6	6.6	0.0536623
15	6.51	404	49.2	0.0449826	5	0.221113	1.4	1	6.51	408.1	5.1	0.0242505
16	7.37	514	62.6	0.0750664	7	0.861155	5.5	3.8	7.37	514.5	7.2	0.06703
17	7.545	541	65.9	0.0150717	18	0.527545	3.4	2.3	7.545	534	18	0.0165356
18	7.662	559	68.1	0.0897869	4	0.56976	3.6	2.5	7.662	558.3	3.8	0.0856128
19	8.45	700	85.3	0.0326771	7	0.383993	2.5	1.7	8.45	700.2	6.7	0.0323247
20	8.65	746	90.9	0.0244778	5	0.185325	1.2	0.8	8.65	746.4	4.5	0.0230178
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	9.3	794	96.7	0.0516626	8	0.608257	3.9	2.7	9.3	793.9	7.6	0.0453083

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.276	39	4.7	0.130244	10	2.19116	12.4	8.3	4.276	39.3	9.9	0.124272
2	4.672	99	12	0.241396	7	2.86601	16.2	10.9	4.672	100.6	7.1	0.227122
3	5.941	303	36.8	0.500412	3	2.86517	16.2	10.9	5.941	302.7	3.2	0.505909
4	5.99	312	37.9	0.349778	4	2.16732	12.2	8.2	5.99	311.5	3.9	0.312937
5	6.029	319	38.8	0.237264	4	1.57236	8.9	6	6.029	319.2	4.1	0.217047
6	6.144	340	41.3	0.0525863	3	0.250528	1.4	0.9	6.144	339.9	2.5	0.055863
7	6.183	347	42.2	0.0477842	3	0.265072	1.5	1	6.183	347	3.3	0.0453706
8	6.216	353	42.9	0.379775	2	1.25028	7.1	4.7	6.216	353.2	1.9	0.376495
9	6.343	376	45.7	0.0972098	6	0.989153	5.6	3.7	6.343	376	6.2	0.0897312
10	6.425	391	47.5	0.0114074	1	0.0121154	0.1	0	6.425	390.9	1	0.0065444
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.469	399	48.5	0.0647532	3	0.279139	1.6	1.1	6.469	399	2.7	0.0586303
12	6.513	407	49.5	0.044537	4	0.390573	2.2	1.5	6.513	405.1	4	0.0554445
13	7.396	520	63.2	0.0665717	7	0.718114	4.1	2.7	7.396	519.8	7.4	0.0549859
14	7.545	543	66	0.041429	15	0.899452	5.1	3.4	7.545	540.3	14.6	0.0348747
15	7.669	562	68.3	0.171114	3	1.00753	5.7	3.8	7.669	562	3.5	0.16305

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	81	9.8	0.0677347	6	0.612162	9.8	7.7	4.55	80.6	5.6	0.0621037
2	5.2	182	22.1	0.0797464	5	0.636024	10.2	8	5.2	181.7	4.7	0.0765543
3	5.85	290	35.2	0.118228	4	0.778068	12.5	9.8	5.85	290	3.7	0.117407
4	6.55	415	50.4	0.0542862	10	0.913294	14.6	11.5	6.55	414.3	9.8	0.0528275
5	7.35	514	62.4	0.042829	5	0.370903	5.9	4.7	7.35	514.3	5.1	0.0410144
6	8.15	637	77.3	0.0499611	8	0.718613	11.5	9	8.15	637.6	8.2	0.0492648
7	8.45	701	85.1	0.0821124	7	0.968961	15.5	12.2	8.45	700.4	6.8	0.0802417
8	8.65	749	90.9	0.051446	5	0.407008	6.5	5.1	8.65	747.7	4.5	0.0507161
9	9.3	797	96.7	0.0825293	6	0.829649	13.3	10.4	9.3	796.6	6	0.0774726

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 80

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 11.300 5.200 11.200 5.850 13.000 6.550 7.900 7.350 5.600 8.150 11.900 8.450 14.700 8.650 6.600 9.300 17.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.139 1.900 4.228 11.100 4.671 19.000 5.936 14.900 5.982 9.900 6.034 8.300 6.153 0.900 6.200 5.700 6.226 5.700 6.340 7.100 6.418 0 6.459 1.200 6.496 2.500 6.627 0.900 7.389 4.200 7.526 2.200 7.650 4.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 17 Return to Lane Navigator
4.550 7.400 5.200 10.000 5.850 11.600 6.550 11.100 7.350 7.800 8.150 13.100 8.450 17.000 8.650 6.300 9.300 15.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.286 6.900 4.550 0.900 4.672 17.100 5.200 1.200 5.850 1.300 5.926 15.500 5.980 11.200 6.023 5.500 6.153 1.500 6.196 1.600 6.229 5.900 6.348 5.000 6.429 0.700 6.472 4.000 6.510 1.400 7.370 5.500 7.545 3.400 7.662 3.600 8.450 2.500 8.650 1.200 9.300 3.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 21 Return to Lane Navigator
4.276 12.400 4.672 16.200 5.941 16.200 5.990 12.200 6.029 8.900 6.144 1.400 6.183 1.500 6.216 7.100 6.343 5.600 6.425 0.100 6.469 1.600 6.513 2.200 7.396 4.100 7.545 5.100 7.669 5.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 15 Return to Lane Navigator
4.550 9.800 5.200 10.200 5.850 12.500 6.550 14.600 7.350 5.900 8.150 11.500 8.450 15.500 8.650 6.500 9.300 13.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Canary Rockfish ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Canary Rockfish ---- Go to Display Gel Lane Navigator.