Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Black Drum  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Pogonias cromis
FDA Seafood List Common Name: Black Drum
FDA Seafood List Market Name: Drum
Unique IEF Gel Code: 19970205gel03
Unique Web Code: bdh01Srr
Tissue Aliquot Code: pogocrom K96-002 #05p Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55151.90.086201150.6552145.94.74.5515.54.60.0808011
25.211314.60.1336140.8581947.86.25.21133.80.128217
35.8522829.50.099156950.77593475.65.85227.84.50.0968693
46.5536547.30.14198881.9221617.413.86.55364.180.135092
56.8540252.10.0287228120.5321984.83.86.85400.311.70.0256899
67.3546660.40.041566350.3387443.12.47.35465.74.70.0408752
78.1559076.40.0980541101.770931612.78.15590.510.40.0956977
88.4564883.90.1544671.8199516.4138.45647.37.10.14556
98.6569890.40.11299240.8601217.86.28.65697.94.40.111114
109.374796.80.10699491.5316813.8119.3744.19.30.0927539
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.645324.10.582155.2222414.110.44.64531.850.592078
24.808557.10.036017350.2875020.80.64.808554.50.0356904
35.27712516.20.19580772.213964.45.277124.46.50.192144
45.32513417.40.17481941.156823.12.35.3251344.20.15407
55.37314318.50.085801140.6199611.71.25.373142.94.10.0844938
65.597185240.086067670.9767032.61.95.597184.76.80.0811618
75.70920626.70.45175443.425829.36.85.709205.94.30.451908
85.76721728.10.24235952.24066.14.55.767217.45.10.247436
95.85323330.20.19181331.141473.12.35.853233.23.30.197888
105.90824431.60.21451441.565384.23.15.908244.44.20.210807
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.96925633.20.26234141.685764.63.45.9692563.70.259104
126.0527235.20.087613450.75219621.56.05271.64.50.0940316
136.29232041.50.021103730.1087960.30.26.292319.93.10.0200153
146.32332642.20.028234940.1726140.50.36.323326.23.50.0275835
156.41934544.70.141209113.168958.66.36.419347.910.60.169274
166.47935746.20.28248553.353829.16.76.479362.94.90.384929
176.5236547.30.4589251.8513753.76.52366.84.90.213953
186.64638349.60.083184730.3976641.10.86.646383.12.80.0811251
196.72639350.90.057477320.235830.60.56.726393.22.30.0573196
206.81440452.30.001779610006.81439810
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.941553.80.054750830.2933940.80.66.9415.530.0552699
226.98542655.20.20153631.050742.82.16.985425.72.90.206022
237.144157.10.38634732.363536.44.77.1440.43.40.397958
247.27746460.10.113731132.702957.35.47.27746212.90.118473
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55222.80.052563950.4410896.14.74.5522.44.70.0530702
25.210813.90.037756390.6318688.86.85.2109.99.30.0381992
35.8523730.60.066629850.5841968.16.35.85236.74.90.0676472
46.5537748.60.13408581.6634923.217.86.55376.77.90.118355
56.8541553.50.02070150.1788072.51.96.85415.15.10.019753
67.3548162.10.040481140.3064124.33.37.35481.64.30.0399083
78.1559376.50.0620492101.0269414.3118.15592.19.60.0602941
88.4565484.40.066725970.858159129.28.45653.37.30.0665008
98.6570390.70.062387950.4922336.95.38.65702.44.60.0607887
109.375096.80.0641888100.99509713.910.79.3746.3100.0563769
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55253.20.057495630.1937770.80.74.5522.73.50.031602
24.607334.30.2856752.758211.910.34.60732.75.40.288638
34.748536.80.008970150.08063790.30.34.74855.35.30.0085261
45.211715.10.0741233141.670227.26.25.2116.713.60.0694281
55.28613317.20.056164830.1934030.80.75.286132.72.80.0384772
65.3314118.20.024547440.1644880.70.65.33140.84.20.0222491
75.70221027.10.21135672.300289.98.65.702209.36.80.190548
85.78322529.10.076519650.6697452.92.55.783224.24.80.078864
95.86924131.10.12210751.080444.745.869240.84.90.123693
105.9425532.90.11388940.8792463.83.35.942554.40.113563
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.99726634.40.16987341.050284.53.95.997266.23.50.168263
126.08928436.70.042743450.4226211.81.66.0892825.20.0460069
136.299325420.029652650.2446971.10.96.299323.65.40.0256187
146.37634043.90.06557760.6566752.82.56.376341.55.80.0638618
156.46835846.30.40140964.1009217.715.36.468359.66.50.357515
166.5537448.30.019739710006.5537010
176.67539050.40.011291930.1970410.80.76.675393.43.30.034137
186.76940251.90.032296630.1718850.70.66.769400.63.10.0317721
196.8441153.10.032746270.3711161.61.46.84414.46.90.0304454
206.96742855.30.087919420.3379181.51.36.967428.42.20.088069
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.08844457.40.29649831.537456.65.77.088443.52.90.29729
227.3547961.90.120393101.985678.67.47.35480.910.20.10953
238.1559476.70.03743270.47543621.88.15596.270.0380943
248.4565584.60.049650170.6391982.82.48.45654.87.50.0481113
258.6570290.70.052118950.46614821.78.65702.35.30.049704
269.375297.20.048245180.5565542.42.19.3749.87.90.0397983
PeakCounter: 26
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.653364.70.40045264.2057513.710.44.65335.35.90.40519
24.823607.80.015000260.1635380.50.44.82360.26.30.0145851
35.26412516.20.13594671.558045.13.95.264123.670.126101
45.32213617.60.14206130.7018432.31.75.322135.72.90.135936
55.37514618.90.056965840.4230711.415.375144.54.30.0559621
65.624193250.046667660.4331731.41.15.624192.25.80.042338
75.7321327.60.46102343.025429.97.55.73213.23.70.458342
85.80422729.40.21720841.651285.44.15.804225.94.50.209235
95.87724131.20.15737330.92072432.35.877240.83.30.158944
105.92925132.50.17010441.252074.13.15.9292514.20.166645
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
115.98626233.90.19894541.272684.23.25.986261.73.60.198679
126.05827635.70.08108350.7130182.31.86.058277.14.70.0864012
136.269317410.030930430.1827870.60.56.269317.43.20.0324756
146.30532441.90.038938620.1519860.50.46.305323.82.30.0380402
156.38333943.90.039870120.1365450.40.36.383338.42.10.0366034
166.4835846.30.42011174.8825215.912.16.48359.16.50.422543
176.62138049.20.053042430.2636090.90.76.621380.42.80.053374
186.71239150.60.05573830.2835240.90.76.712391.330.0541161
196.78640051.70.04728530.2732640.90.76.786399.23.40.0457807
206.89841453.60.047475630.2742020.90.76.898414.43.40.0451526
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
216.981425550.2150931.146473.72.86.981425.23.20.203359
227.09444056.90.41784242.500078.26.27.094439.83.50.402995
237.39848162.20.186456144.2239613.810.57.398481.813.70.174459
PeakCounter: 23
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55202.60.043285360.46309653.64.5521.36.10.0431509
25.211114.30.054198970.6699687.25.35.2112.670.0543256
35.8523630.40.093145250.7707588.36.15.85236.54.70.0926173
46.5537147.80.12927671.5307516.512.16.55369.87.20.119754
56.8540552.20.036262640.2723232.92.16.85405.44.40.0349454
67.3547160.70.077312840.5674726.14.57.35470.64.30.0746027
78.1559176.20.0901216111.6597217.913.18.15591.411.30.082942
88.4565384.10.15089471.8066719.514.28.45651.770.145375
98.6570090.20.096766240.7481848.15.98.657004.40.0957493
109.375497.20.069684370.7765618.46.19.37526.90.0637365
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 8.65 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 103
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 26 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 23 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln