RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Pollock ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Pollachius virens
FDA Seafood List Common Name: Pollock
FDA Seafood List Market Name: Pollock
Unique IEF Gel Code: 19960118gel05
Unique Web Code: aph04Srr
Tissue Aliquot Code: pollvire A96-002 #04a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	19	2.5	0.0758736	4	0.486503	13	7.8	4.55	19.7	3.7	0.0732493
2	5.2	122	16.1	0.0505743	4	0.314893	8.4	5	5.2	122.1	3.5	0.0503555
3	5.85	226	29.9	0.0290522	4	0.228621	6.1	3.6	5.85	224.9	4.4	0.0290844
4	6.55	347	45.8	0.0230544	8	0.292715	7.8	4.7	6.55	344.3	8.5	0.0194464
5	7.35	437	57.7	0.0445144	3	0.247095	6.6	3.9	7.35	436.9	3.1	0.0448421
6	8.15	555	73.3	0.047396	6	0.466489	12.5	7.4	8.15	555.5	5.7	0.0459243
7	8.45	618	81.6	0.0722276	5	0.647296	17.3	10.3	8.45	618.4	5.2	0.06957
8	8.65	669	88.4	0.0596041	4	0.398894	10.7	6.4	8.65	668.1	3.8	0.0598629
9	9.3	735	97.1	0.0809674	5	0.658258	17.6	10.5	9.3	734.2	4.7	0.0797179

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.592	25	3.3	0.0098606	3	0.0431218	0.3	0.2	4.592	25.4	2.6	0.0093454
2	4.752	50	6.6	0.0348517	4	0.250021	1.9	1.3	4.752	50.6	4.2	0.0334686
3	4.944	80	10.6	0.012261	8	0.139533	1	0.7	4.944	82.3	7.7	0.0102786
4	5.072	100	13.2	0.0403699	4	0.286174	2.1	1.5	5.072	100	4.4	0.037035
5	5.194	119	15.7	0.0316377	5	0.276812	2.1	1.5	5.194	119.6	4.7	0.0329191
6	5.293	135	17.8	0.370733	3	2.0284	15.1	10.7	5.293	135.7	3.2	0.353215
7	5.424	156	20.6	0.173997	3	0.803748	6	4.3	5.424	155.6	2.9	0.157971
8	5.499	168	22.2	0.0185944	6	0.16872	1.3	0.9	5.499	168.2	5.5	0.0171865
9	5.623	188	24.8	0.0121235	3	0.0574012	0.4	0.3	5.623	187.3	2.9	0.0113569
10	5.698	200	26.4	0.0154735	5	0.125385	0.9	0.7	5.698	201.4	4.9	0.0143877
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.894	232	30.6	0.0202459	8	0.296377	2.2	1.6	5.894	234.5	8.2	0.0203792
12	5.975	246	32.5	0.0197873	3	0.0962477	0.7	0.5	5.975	246.3	3.4	0.0158904
13	6.04	257	33.9	0.291859	3	1.39784	10.4	7.4	6.04	256.9	2.8	0.28521
14	6.11	269	35.5	0.142402	7	1.15258	8.6	6.1	6.11	267.1	6.5	0.0996519
15	6.127	272	35.9	0.129867	7	1.03076	7.7	5.5	6.127	274.7	6.8	0.0853645
16	6.215	287	37.9	0.131543	3	0.543215	4.1	2.9	6.215	286.7	2.6	0.118519
17	6.279	298	39.4	0.0352911	3	0.167657	1.3	0.9	6.279	297.3	2.5	0.0375591
18	6.314	304	40.2	0.0717761	2	0.294888	2.2	1.6	6.314	304	2.3	0.0712786
19	6.384	316	41.7	0.0235023	4	0.133104	1	0.7	6.384	316.1	3.5	0.0214418
20	6.465	330	43.6	0.131536	3	0.600509	4.5	3.2	6.465	330.2	2.7	0.12708
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.541	343	45.3	0.0162024	3	0.100157	0.7	0.5	6.541	343.1	3.4	0.016816
22	9.075	711	93.9	0.101189	14	2.45731	18.3	13	9.075	709.6	13.8	0.100135
23	9.195	723	95.5	0.113158	6	0.955973	7.1	5.1	9.195	725.2	6.3	0.0852499

PeakCounter: 23

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	16	2.1	0.0515466	4	0.326315	10	5.6	4.55	15.8	3.6	0.0508141
2	5.2	116	15.4	0.0303427	5	0.255931	7.8	4.4	5.2	114.4	5.4	0.0269122
3	5.85	221	29.3	0.0284266	5	0.240259	7.4	4.1	5.85	222.4	5	0.0269047
4	6.55	340	45	0.0309506	7	0.36997	11.3	6.3	6.55	338.8	7.3	0.0284752
5	7.35	436	57.7	0.0310348	4	0.180049	5.5	3.1	7.35	436.3	3.7	0.0272216
6	8.15	554	73.4	0.041047	5	0.391955	12	6.7	8.15	554	5.5	0.040515
7	8.45	617	81.7	0.0455242	6	0.447753	13.7	7.7	8.45	616.1	5.6	0.0448033
8	8.65	666	88.2	0.0547505	5	0.441332	13.5	7.5	8.65	665.8	4.9	0.0508251
9	9.3	730	96.7	0.068027	5	0.615132	18.8	10.5	9.3	729.8	5.4	0.0647555

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	21	2.8	0.0291547	3	0.166005	1.3	1.2	4.55	21.3	3.3	0.0283144
2	4.671	40	5.3	0.0683187	9	1.00364	7.9	7	4.671	39.1	9.2	0.0613738
3	4.779	57	7.6	0.0450269	11	0.866852	6.9	6.1	4.779	55.6	10.9	0.0450538
4	5.085	105	13.9	0.0373938	13	0.89928	7.1	6.3	5.085	98.1	13.3	0.0381974
5	5.2	123	16.3	0.0133417	1	0.0158699	0.1	0.1	5.2	123.2	1.2	0.0074257
6	5.3	140	18.6	0.166041	4	1.06056	8.4	7.4	5.3	139.7	3.8	0.156556
7	5.417	160	21.2	0.0510997	9	0.606823	4.8	4.2	5.417	156.2	9.2	0.0371166
8	5.61	193	25.6	0.0093705	3	0.0491329	0.4	0.3	5.61	192.4	3	0.0092908
9	5.674	204	27.1	0.0142895	4	0.0803699	0.6	0.6	5.674	204.4	3.8	0.012078
10	5.85	234	31	0.0223392	2	0.0981449	0.8	0.7	5.85	233.9	2.4	0.0232969
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.912	244	32.4	0.0268854	4	0.160336	1.3	1.1	5.912	243	4	0.0226091
12	6.006	259	34.4	0.163238	3	0.931834	7.4	6.5	6.006	258.6	3.4	0.15448
13	6.088	272	36.1	0.0706625	7	0.769835	6.1	5.4	6.088	273.1	6.6	0.0654496
14	6.2	290	38.5	0.0797135	3	0.456163	3.6	3.2	6.2	289.1	3.4	0.0756877
15	6.3	306	40.6	0.0325756	2	0.119068	0.9	0.8	6.3	306.1	2	0.0330971
16	6.369	317	42	0.0220472	2	0.0951781	0.8	0.7	6.369	317.4	2.4	0.0224187
17	6.463	332	44	0.0856647	3	0.408989	3.2	2.9	6.463	331.5	2.9	0.0809381
18	6.55	346	45.9	0.0200811	4	0.122046	1	0.9	6.55	346.5	3.6	0.0190174
19	7.35	439	58.2	0.0285384	4	0.201436	1.6	1.4	7.35	439	4.3	0.0266575
20	8.15	558	74	0.0226273	16	0.741531	5.9	5.2	8.15	546.9	15.5	0.0269337
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	8.45	616	81.7	0.0454594	6	0.432532	3.4	3	8.45	615.9	5.7	0.0430476
22	8.65	667	88.5	0.0347174	4	0.20622	1.6	1.4	8.65	666.4	3.7	0.0317818
23	9.021	703	93.2	0.0857292	13	1.81672	14.4	12.7	9.021	703.3	13	0.0791368
24	9.197	720	95.5	0.0986231	9	1.32307	10.5	9.2	9.197	721.2	9	0.0829038

PeakCounter: 24

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.582	24	3.2	0.101964	9	1.40452	12.2	9.2	4.582	24.5	9.3	0.0849929
2	4.75	50	6.6	0.0563598	17	1.86227	16.2	12.2	4.75	43.2	16.7	0.062882
3	5.042	95	12.6	0.0385803	13	0.844436	7.4	5.5	5.042	94.7	13.4	0.0355827
4	5.209	121	16.1	0.0016593	4	0.0005437	0	0	5.209	121	4	7.67E-5
5	5.325	140	18.6	0.140741	3	0.695011	6.1	4.6	5.325	140	3	0.130707
6	5.458	162	21.5	0.0533358	3	0.237512	2.1	1.6	5.458	162	3	0.0446675
7	5.634	191	25.4	0.0099607	3	0.0453481	0.4	0.3	5.634	192.2	2.6	0.009834
8	5.877	231	30.7	0.0126505	8	0.173336	1.5	1.1	5.877	227.8	8.4	0.0116595
9	5.955	244	32.4	0.0235829	2	0.081177	0.7	0.5	5.955	244.6	1.9	0.0237722
10	6.021	255	33.9	0.121773	3	0.550479	4.8	3.6	6.021	255.5	2.6	0.118442
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.099	268	35.6	0.0611764	3	0.223361	1.9	1.5	6.099	267.5	2.6	0.047915
12	6.123	272	36.1	0.0361004	5	0.310605	2.7	2	6.123	273.1	5	0.0348715
13	6.208	286	38	0.0627784	5	0.373543	3.3	2.4	6.208	284.9	4.8	0.0440949
14	6.31	303	40.2	0.0416175	4	0.202306	1.8	1.3	6.31	302.6	4	0.0288798
15	6.382	315	41.8	0.0219711	15	0.375064	3.3	2.5	6.382	322.3	14.6	0.0145055
16	6.472	330	43.8	0.104609	3	0.507901	4.4	3.3	6.472	329.6	3.2	0.0897102
17	6.576	346	45.9	0.0094884	2	0.0173946	0.2	0.1	6.576	345.1	1.8	0.0053149
18	6.686	359	47.7	0.0149334	6	0.128528	1.1	0.8	6.686	360.7	6.3	0.011526
19	9.044	705	93.6	0.104007	15	2.84839	24.8	18.7	9.044	708.3	15.2	0.105564
20	9.198	720	95.6	0.112013	5	0.594504	5.2	3.9	9.198	722	5.1	0.0658028

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	19	2.5	0.0284515	5	0.298407	8.6	5.3	4.55	18.7	5.5	0.030837
2	5.2	118	15.6	0.0170063	6	0.17179	4.9	3.1	5.2	117.1	6.4	0.0151772
3	5.85	221	29.3	0.0218349	9	0.274475	7.9	4.9	5.85	221.6	8.9	0.0174412
4	6.55	342	45.4	0.0326388	10	0.661203	19	11.8	6.55	337.9	9.7	0.0384303
5	7.35	437	58	0.0275914	3	0.143945	4.1	2.6	7.35	436.8	3.2	0.0255343
6	8.15	553	73.3	0.0400856	6	0.421091	12.1	7.5	8.15	553.8	6.2	0.038185
7	8.45	616	81.7	0.0535915	6	0.591113	17	10.6	8.45	615.5	6.5	0.0514935
8	8.65	668	88.6	0.0561155	5	0.415462	12	7.4	8.65	667.5	4.6	0.0508986
9	9.3	732	97.1	0.0558838	6	0.497863	14.3	8.9	9.3	731.7	5.7	0.0492184

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 94

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 13.000 5.200 8.400 5.850 6.100 6.550 7.800 7.350 6.600 8.150 12.500 8.450 17.300 8.650 10.700 9.300 17.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.592 0.300 4.752 1.900 4.944 1.000 5.072 2.100 5.194 2.100 5.293 15.100 5.424 6.000 5.499 1.300 5.623 0.400 5.698 0.900 5.894 2.200 5.975 0.700 6.040 10.400 6.110 8.600 6.127 7.700 6.215 4.100 6.279 1.300 6.314 2.200 6.384 1.000 6.465 4.500 6.541 0.700 9.075 18.300 9.195 7.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 23 Return to Lane Navigator
4.550 10.000 5.200 7.800 5.850 7.400 6.550 11.300 7.350 5.500 8.150 12.000 8.450 13.700 8.650 13.500 9.300 18.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.550 1.300 4.671 7.900 4.779 6.900 5.085 7.100 5.200 0.100 5.300 8.400 5.417 4.800 5.610 0.400 5.674 0.600 5.850 0.800 5.912 1.300 6.006 7.400 6.088 6.100 6.200 3.600 6.300 0.900 6.369 0.800 6.463 3.200 6.550 1.000 7.350 1.600 8.150 5.900 8.450 3.400 8.650 1.600 9.021 14.400 9.197 10.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 24 Return to Lane Navigator
4.582 12.200 4.750 16.200 5.042 7.400 5.209 0 5.325 6.100 5.458 2.100 5.634 0.400 5.877 1.500 5.955 0.700 6.021 4.800 6.099 1.900 6.123 2.700 6.208 3.300 6.310 1.800 6.382 3.300 6.472 4.400 6.576 0.200 6.686 1.100 9.044 24.800 9.198 5.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 20 Return to Lane Navigator
4.550 8.600 5.200 4.900 5.850 7.900 6.550 19.000 7.350 4.100 8.150 12.100 8.450 17.000 8.650 12.000 9.300 14.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

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Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Pollock ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Pollock ---- Go to Display Gel Lane Navigator.