RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Pollock ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Pollachius virens
FDA Seafood List Common Name: Pollock
FDA Seafood List Market Name: Pollock
Unique IEF Gel Code: 19930823gel03
Unique Web Code: apg02Srr
Tissue Aliquot Code: pollvire 001 #1 Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Co-Migrated	Lane f Specimen	Lane g pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	58	4.9	0.338529	5	2.99582	20.3	16.1	3.5	58	5.3	0.318017
2	4.55	208	17.5	0.0920786	7	1.07391	7.3	5.8	4.55	208	6.7	0.0900206
3	5.2	283	23.9	0.0899221	6	0.94975	6.4	5.1	5.2	283.1	6.1	0.0872808
4	5.85	427	36	0.178191	7	1.98149	13.4	10.7	5.85	426.2	6.6	0.168281
5	6.55	624	52.6	0.182649	5	1.63606	11.1	8.8	6.55	623.9	5.2	0.176339
6	6.85	662	55.8	0.0715181	7	0.774371	5.2	4.2	6.85	662.7	6.9	0.0636636
7	7.35	761	64.2	0.170965	6	1.69841	11.5	9.1	7.35	760.8	5.7	0.168941
8	8.15	919	77.5	0.0771135	11	1.36434	9.2	7.3	8.15	919.3	10.7	0.072134
9	8.45	993	83.7	0.0703318	5	0.660513	4.5	3.6	8.45	993.5	5.4	0.0691411
10	8.65	1032	87	0.0367452	5	0.31933	2.2	1.7	8.65	1031.6	5.2	0.0347593
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1163	98.1	0.161727	5	1.31942	8.9	7.1	9.3	1162.7	4.7	0.156792

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	55	4.6	0.498091	7	5.60977	18	16.5	3.5	55.1	7.1	0.442962
2	4.168	158	13.3	0.281584	5	2.74165	8.8	8.1	4.168	158.7	5.4	0.286174
3	4.55	217	18.3	0.0898376	7	0.97302	3.1	2.9	4.55	216.9	6.8	0.0804846
4	4.762	245	20.7	0.056044	5	0.504274	1.6	1.5	4.762	244.8	5.2	0.0545799
5	4.935	268	22.6	0.128689	6	1.32306	4.3	3.9	4.935	267.6	5.7	0.131665
6	5.079	287	24.2	0.0250022	7	0.301639	1	0.9	5.079	290.4	7.5	0.0227276
7	5.2	303	25.5	0.08962	5	0.82021	2.6	2.4	5.2	303.4	5.3	0.0869105
8	5.279	320	27	0.270497	4	1.71003	5.5	5	5.279	319.7	3.6	0.265074
9	5.409	348	29.3	0.104146	5	0.857789	2.8	2.5	5.409	347.6	4.6	0.104709
10	5.511	370	31.2	0.0120891	8	0.1744	0.6	0.5	5.511	368	7.9	0.0125244
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.571	383	32.3	0.00707524	3	0.0479648	0.2	0.1	5.571	383.8	3.3	0.00823096
12	5.678	406	34.2	0.0167543	5	0.130024	0.4	0.4	5.678	406.1	5.3	0.0139069
13	5.85	443	37.4	0.0964666	10	1.86568	6	5.5	5.85	438.7	10	0.105733
14	5.988	481	40.6	0.152413	5	1.43652	4.6	4.2	5.988	480.3	5.1	0.158964
15	6.053	499	42.1	0.174235	4	1.26422	4.1	3.7	6.053	499	4.1	0.175359
16	6.122	518	43.7	0.0888692	6	0.838422	2.7	2.5	6.122	516.7	5.8	0.0820473
17	6.169	531	44.8	0.136104	4	0.969539	3.1	2.9	6.169	530.9	4	0.136498
18	6.234	549	46.3	0.00842135	18	0.247499	0.8	0.7	6.234	539.1	18	0.00775772
19	6.292	565	47.6	0.0287787	4	0.183494	0.6	0.5	6.292	564.4	4	0.0258051
20	6.387	591	49.8	0.0101807	10	0.252699	0.8	0.7	6.387	602.4	10	0.0142224
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.459	611	51.5	0.0750459	4	0.541928	1.7	1.6	6.459	611.6	4.3	0.0711223
22	6.55	636	53.6	0.048345	9	0.74831	2.4	2.2	6.55	634.5	9.2	0.0459436
23	6.7	656	55.3	0.0151011	4	0.0662821	0.2	0.2	6.7	655.3	3.9	0.00957495
24	6.85	676	57	0.0324021	4	0.233022	0.7	0.7	6.85	676	4	0.0327129
25	7.35	771	65	0.184259	5	1.70494	5.5	5	7.35	771.3	5.1	0.187001
26	8.15	928	78.2	0.0198344	9	0.288513	0.9	0.8	8.15	929.2	9.4	0.0173732
27	8.45	1003	84.6	0.0582343	5	0.55286	1.8	1.6	8.45	1002	5.4	0.0577148
28	8.65	1043	87.9	0.0575335	10	0.922536	3	2.7	8.65	1044.4	9.7	0.0534998
29	8.81	1073	90.5	0.105756	16	2.66878	8.6	7.9	8.81	1073.1	15.6	0.0962884
30	9.3	1165	98.2	0.100316	8	1.10403	3.6	3.3	9.3	1163.4	8.1	0.0769156

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.477	52	4.4	0.175177	7	2.28769	16.5	13.7	3.477	50.6	7.3	0.176314
2	4.15	154	13	0.246268	5	2.1667	15.6	13	4.15	153.7	4.9	0.250129
3	4.726	237	20	0.0396454	8	0.476065	3.4	2.9	4.726	239.4	7.8	0.0343375
4	4.898	259	21.8	0.11267	6	1.11193	8	6.7	4.898	259	5.6	0.111647
5	5.248	308	26	0.220411	4	1.36583	9.9	8.2	5.248	307.9	3.7	0.21041
6	5.377	336	28.3	0.0902728	4	0.627486	4.5	3.8	5.377	336.3	4.1	0.0866985
7	5.963	470	39.6	0.155857	4	1.13663	8.2	6.8	5.963	469.6	4.1	0.155236
8	6.028	488	41.1	0.190787	3	1.10115	7.9	6.6	6.028	488.6	3.4	0.185146
9	6.096	507	42.7	0.107275	5	0.741229	5.3	4.5	6.096	506.7	4.7	0.0890214
10	6.143	520	43.8	0.148505	4	1.07461	7.8	6.5	6.143	519.8	4.3	0.141361
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.254	551	46.5	0.0321093	4	0.210174	1.5	1.3	6.254	550	4.3	0.0272957
12	6.308	566	47.7	0.00352564	2	0.0116174	0.1	0.1	6.308	565	2	0.00325422
13	6.365	582	49.1	0.0219451	14	0.889357	6.4	5.3	6.365	593.2	14.1	0.035529
14	6.433	601	50.7	0.085761	3	0.293343	2.1	1.8	6.433	600.7	3	0.0548203
15	6.624	643	54.2	0.0267131	8	0.319883	2.3	1.9	6.624	642.7	7.6	0.0237371
16	8.791	1065	89.8	0.00703999	4	0.0447548	0.3	0.3	8.791	1065	4	0.00626774

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 8
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	58	4.9	0.581215	6	5.35195	30.9	25.1	3.5	58.5	5.9	0.515487
2	4.55	214	18	0.121817	6	1.24187	7.2	5.8	4.55	214	6.3	0.110555
3	5.2	294	24.7	0.128193	6	1.26068	7.3	5.9	5.2	294.4	5.8	0.12309
4	5.85	436	36.7	0.167752	7	2.12166	12.2	10	5.85	434.4	7.4	0.161762
5	6.55	633	53.2	0.180634	5	1.55518	9	7.3	6.55	633	4.9	0.17859
6	6.85	670	56.3	0.0656054	7	0.699069	4	3.3	6.85	670.2	6.6	0.0599419
7	7.35	765	64.3	0.17224	6	1.72458	9.9	8.1	7.35	764.6	5.7	0.169803
8	8.15	922	77.5	0.068051	9	1.03617	6	4.9	8.15	922.2	8.8	0.0662935
9	8.45	992	83.4	0.0778249	5	0.748476	4.3	3.5	8.45	991.9	5.5	0.0769395
10	8.65	1034	87	0.0639131	5	0.528172	3	2.5	8.65	1034.2	5.1	0.0585551
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1166	98.1	0.0547775	11	1.07429	6.2	5	9.3	1162.8	11.1	0.0548364

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 8
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 9
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	59	5	0.243744	7	2.87969	8.6	7.6	3.5	59.2	7	0.230598
2	4.146	155	13	0.292549	5	2.90888	8.6	7.7	4.146	156	5.5	0.299759
3	4.55	215	18.1	0.0802603	6	0.744247	2.2	2	4.55	215.4	5.6	0.0746736
4	4.767	241	20.3	0.0458382	6	0.464101	1.4	1.2	4.767	240.9	5.7	0.0462539
5	4.942	262	22	0.126662	5	1.22742	3.6	3.2	4.942	261.6	5.3	0.131048
6	5.083	279	23.5	0.0272144	3	0.0995198	0.3	0.3	5.083	278.9	2.8	0.0203628
7	5.2	293	24.6	0.0707426	11	1.31023	3.9	3.5	5.2	295.5	11.1	0.066897
8	5.268	308	25.9	0.207576	6	1.76661	5.3	4.7	5.268	307.8	5.6	0.178061
9	5.391	335	28.2	0.0790403	4	0.573113	1.7	1.5	5.391	335	4.1	0.0788421
10	5.491	357	30	0.0113895	9	0.133023	0.4	0.4	5.491	358.1	9	0.00830274
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.591	379	31.9	0.0048322	24	0.0240527	0.1	0.1	5.591	368.1	24	0.000565438
12	5.673	397	33.4	0.0156788	6	0.136059	0.4	0.4	5.673	396.6	6.2	0.0123341
13	5.764	417	35.1	0.0167764	11	0.443941	1.3	1.2	5.764	424.5	10.8	0.0231314
14	5.85	436	36.7	0.128003	4	0.925372	2.8	2.4	5.85	435.6	4.4	0.119198
15	5.94	460	38.7	0.230234	8	3.26199	9.7	8.6	5.94	459.7	7.8	0.234866
16	6.03	484	40.7	0.235968	5	2.16469	6.4	5.7	6.03	484	5.4	0.224615
17	6.116	507	42.6	0.155727	6	1.51672	4.5	4	6.116	506	6.2	0.137451
18	6.164	520	43.7	0.190323	4	1.36252	4.1	3.6	6.164	520.3	4.2	0.181956
19	6.303	557	46.8	0.0784344	4	0.482334	1.4	1.3	6.303	556.9	3.7	0.0733755
20	6.468	601	50.5	0.123816	4	0.808105	2.4	2.1	6.468	600.7	3.9	0.116517
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.55	623	52.4	0.0646122	8	0.88542	2.6	2.3	6.55	621	8	0.0621111
22	6.85	664	55.8	0.0440999	6	0.437807	1.3	1.2	6.85	664.6	6	0.0413353
23	7.35	763	64.2	0.166389	5	1.52859	4.5	4	7.35	762.4	5.1	0.167568
24	8.15	927	78	0.0230919	10	0.344559	1	0.9	8.15	927.7	10.1	0.0193236
25	8.45	999	84	0.0495792	6	0.52589	1.6	1.4	8.45	997.8	6.1	0.0489114
26	8.65	1038	87.3	0.0717425	13	1.49505	4.4	4	8.65	1041.9	13.1	0.0645096
27	8.817	1072	90.2	0.150217	16	3.72934	11.1	9.9	8.817	1071.7	16.2	0.13
28	9.3	1170	98.4	0.21771	4	1.45549	4.3	3.8	9.3	1170.1	4.3	0.189191

PeakCounter: 28

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 9
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 10
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.179	161	13.5	0.251023	5	2.3057	14.4	12.2	4.179	160.9	5.2	0.250402
2	4.8	248	20.9	0.0422164	8	0.554739	3.5	2.9	4.8	249.3	7.7	0.0407339
3	4.99	272	22.9	0.110547	6	1.15598	7.2	6.1	4.99	270.9	5.9	0.110167
4	5.156	293	24.6	0.0185589	5	0.13634	0.9	0.7	5.156	292	5.2	0.0146571
5	5.216	302	25.4	0.0149629	11	0.383877	2.4	2	5.216	308.3	11.3	0.0191337
6	5.292	319	26.8	0.177421	5	1.41897	8.9	7.5	5.292	318.6	4.8	0.167274
7	5.414	346	29.1	0.0727302	5	0.629731	3.9	3.3	5.414	345.1	4.8	0.0746029
8	5.508	367	30.9	0.00292338	1	0.00503595	0	0	5.508	367.4	0.7	0.0038086
9	5.576	382	32.1	0.00293048	7	0.0540477	0.3	0.3	5.576	382.7	6.6	0.00464049
10	5.783	428	36	0.0099325	7	0.0916828	0.6	0.5	5.783	426	6.7	0.00776951
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.861	446	37.5	0.00865246	3	0.0505304	0.3	0.3	5.861	445.1	3.5	0.00819559
12	5.942	468	39.4	0.208897	7	2.76246	17.3	14.6	5.942	467.2	7.4	0.210799
13	6.026	491	41.3	0.205606	6	1.93408	12.1	10.2	6.026	491.1	5.6	0.195849
14	6.104	512	43.1	0.133371	7	1.53326	9.6	8.1	6.104	511.4	6.9	0.124553
15	6.151	525	44.2	0.165166	4	1.22313	7.7	6.5	6.151	525	4.3	0.159357
16	6.28	560	47.1	0.0533545	4	0.328876	2.1	1.7	6.28	559.7	3.5	0.0524486
17	6.438	603	50.7	0.113053	6	1.03545	6.5	5.5	6.438	602.2	5.9	0.0991853
18	8.855	1084	91.2	0.0227442	10	0.368752	2.3	1.9	8.855	1082.5	10	0.0208677

PeakCounter: 18

**DISPLAY GEL LANE LETTER:** Lane g
**DIGITAL REPORT GEL LANE:** 10
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 11
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	60	5	0.222692	6	2.19559	17.9	14.4	3.5	60.5	5.9	0.210005
2	4.55	218	18.3	0.0807283	7	0.961366	7.8	6.3	4.55	219.1	7	0.0775302
3	5.2	304	25.5	0.0885983	7	1.02795	8.4	6.8	5.2	303.1	6.8	0.0853583
4	5.85	450	37.8	0.0980105	13	2.09499	17	13.8	5.85	447.6	12.6	0.093641
5	6.55	644	54.1	0.113679	7	1.44055	11.7	9.5	6.55	645.1	7	0.116487
6	6.85	683	57.3	0.0293861	10	0.541057	4.4	3.6	6.85	686.7	10.4	0.0293038
7	7.35	779	65.4	0.0966166	9	1.61049	13.1	10.6	7.35	779	9.3	0.0974926
8	8.15	939	78.8	0.0542788	12	1.0593	8.6	7	8.15	939	11.8	0.0505676
9	8.45	1008	84.6	0.0681778	6	0.74759	6.1	4.9	8.45	1007.7	6.3	0.066897
10	8.65	1049	88.1	0.0408099	7	0.477783	3.9	3.1	8.65	1048.8	6.7	0.0400958
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1174	98.6	0.0365204	4	0.131818	1.1	0.9	9.3	1174.4	3.6	0.0204395

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f g

MK CO SP MK CO SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 125

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 20.300 4.550 7.300 5.200 6.400 5.850 13.400 6.550 11.100 6.850 5.200 7.350 11.500 8.150 9.200 8.450 4.500 8.650 2.200 9.300 8.900 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 5 PeakCount: 11 Return to Lane Navigator
3.500 18.000 4.168 8.800 4.550 3.100 4.762 1.600 4.935 4.300 5.079 1.000 5.200 2.600 5.279 5.500 5.409 2.800 5.511 0.600 5.571 0.200 5.678 0.400 5.850 6.000 5.988 4.600 6.053 4.100 6.122 2.700 6.169 3.100 6.234 0.800 6.292 0.600 6.387 0.800 6.459 1.700 6.550 2.400 6.700 0.200 6.850 0.700 7.350 5.500 8.150 0.900 8.450 1.800 8.650 3.000 8.810 8.600 9.300 3.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 6 PeakCount: 30 Return to Lane Navigator
3.477 16.500 4.150 15.600 4.726 3.400 4.898 8.000 5.248 9.900 5.377 4.500 5.963 8.200 6.028 7.900 6.096 5.300 6.143 7.800 6.254 1.500 6.308 0.100 6.365 6.400 6.433 2.100 6.624 2.300 8.791 0.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 7 PeakCount: 16 Return to Lane Navigator
3.500 30.900 4.550 7.200 5.200 7.300 5.850 12.200 6.550 9.000 6.850 4.000 7.350 9.900 8.150 6.000 8.450 4.300 8.650 3.000 9.300 6.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 8 PeakCount: 11 Return to Lane Navigator
3.500 8.600 4.146 8.600 4.550 2.200 4.767 1.400 4.942 3.600 5.083 0.300 5.200 3.900 5.268 5.300 5.391 1.700 5.491 0.400 5.591 0.100 5.673 0.400 5.764 1.300 5.850 2.800 5.940 9.700 6.030 6.400 6.116 4.500 6.164 4.100 6.303 1.400 6.468 2.400 6.550 2.600 6.850 1.300 7.350 4.500 8.150 1.000 8.450 1.600 8.650 4.400 8.817 11.100 9.300 4.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 8 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 9 PeakCount: 28 Return to Lane Navigator
4.179 14.400 4.800 3.500 4.990 7.200 5.156 0.900 5.216 2.400 5.292 8.900 5.414 3.900 5.508 0 5.576 0.300 5.783 0.600 5.861 0.300 5.942 17.300 6.026 12.100 6.104 9.600 6.151 7.700 6.280 2.100 6.438 6.500 8.855 2.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 9 LANE TYPE: Specimen ACTUAL GEL LANE: 10 PeakCount: 18 Return to Lane Navigator
3.500 17.900 4.550 7.800 5.200 8.400 5.850 17.000 6.550 11.700 6.850 4.400 7.350 13.100 8.150 8.600 8.450 6.100 8.650 3.900 9.300 1.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 10 LANE TYPE: pI Markers ACTUAL GEL LANE: 11 PeakCount: 11 Return to Lane Navigator

RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Pollock ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Pollock ---- Go to Display Gel Lane Navigator.