RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Cod ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Gadus morhua
FDA Seafood List Common Name: Atlantic Cod
FDA Seafood List Market Name: Cod
Unique IEF Gel Code: 19960122gel06
Unique Web Code: ach03Srr
Tissue Aliquot Code: gadumorh A96-002 #01a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	44	5.6	0.0445798	7	0.529282	13.4	5.7	4.55	42.7	6.9	0.0431563
2	5.2	119	15.3	0.0310424	5	0.284048	7.2	3.1	5.2	120.1	5.4	0.029611
3	5.85	219	28.1	0.0270523	6	0.23525	6	2.5	5.85	217.3	5.8	0.022967
4	6.55	349	44.7	0.0512582	6	0.38891	9.9	4.2	6.55	347.6	5.7	0.0382407
5	7.35	455	58.3	0.0478756	4	0.311215	7.9	3.4	7.35	455.4	3.7	0.0480468
6	8.15	575	73.7	0.0376259	6	0.435062	11	4.7	8.15	573.2	6.5	0.0380016
7	8.45	639	81.9	0.0660782	8	0.832505	21.1	9	8.45	641	7.8	0.0605761
8	8.65	694	89	0.054323	5	0.42738	10.8	4.6	8.65	693	5.2	0.0466064
9	9.3	758	97.2	0.0653339	5	0.497346	12.6	5.4	9.3	757.1	4.6	0.0611997

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.352	17	2.2	0.016566	5	0.12986	1.3	0.8	4.352	17.3	4.8	0.015126
2	4.538	40	5.1	0.108853	11	1.90131	19.3	11.1	4.538	42.5	11.5	0.0936558
3	5.168	118	15.1	0.0216979	4	0.153391	1.6	0.9	5.168	117.2	4.1	0.0212074
4	5.276	133	17.1	0.207328	5	1.83847	18.6	10.7	5.276	132	5	0.208433
5	5.379	148	19	0.110401	6	1.1682	11.8	6.8	5.379	146.5	6.1	0.10858
6	5.51	167	21.4	0.0504054	3	0.269867	2.7	1.6	5.51	167.1	2.8	0.0541593
7	5.668	190	24.4	0.0508285	3	0.295976	3	1.7	5.668	190.6	3.4	0.0492578
8	5.819	212	27.2	0.116163	5	1.03909	10.5	6.1	5.819	213.1	5.2	0.11371
9	5.931	232	29.8	0.0674884	9	0.89142	9	5.2	5.931	232.1	8.6	0.0584662
10	5.998	245	31.5	0.0093984	12	0.0908083	0.9	0.5	5.998	246.9	12	0.0042698
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.066	258	33.1	0.0122405	2	0.0315392	0.3	0.2	6.066	257.2	2.3	0.0076747
12	6.097	264	33.9	0.0124235	3	0.0571522	0.6	0.3	6.097	264.7	3.2	0.0101506
13	6.207	285	36.6	0.0826027	3	0.478538	4.9	2.8	6.207	285.2	3.5	0.0773892
14	6.285	300	38.5	0.0455125	2	0.187525	1.9	1.1	6.285	300.1	2.2	0.0471911
15	6.337	310	39.8	0.0367793	5	0.237316	2.4	1.4	6.337	311.6	4.6	0.0288103
16	6.409	324	41.6	0.100391	7	1.09206	11.1	6.4	6.409	325.8	6.8	0.0908549

PeakCounter: 16

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	40	5.1	0.0626174	4	0.45781	12	5.5	4.55	39.8	4.4	0.0584274
2	5.2	126	16.2	0.0379896	4	0.252987	6.7	3	5.2	126.2	4.1	0.0347597
3	5.85	215	27.6	0.025122	3	0.116436	3.1	1.4	5.85	214.8	2.9	0.0229266
4	6.55	354	45.5	0.0305472	18	0.751053	19.7	9	6.55	360.9	18	0.0235413
5	7.35	452	58.1	0.0589277	3	0.312085	8.2	3.7	7.35	452.2	3.1	0.0574468
6	8.15	569	73.1	0.0463747	7	0.500663	13.2	6	8.15	569.8	6.9	0.0406851
7	8.45	639	82.1	0.0426889	8	0.511174	13.4	6.1	8.45	637.6	8	0.0361891
8	8.65	691	88.8	0.0453727	5	0.344999	9.1	4.1	8.65	690.2	4.6	0.0419261
9	9.3	754	96.9	0.0596452	5	0.556286	14.6	6.7	9.3	754.1	5.4	0.0580058

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.476	31	4	0.0470087	4	0.3114	4.1	3.2	4.476	30.7	4.2	0.0413538
2	4.55	41	5.2	0.0136283	3	0.0584381	0.8	0.6	4.55	41.6	2.5	0.0129845
3	4.698	61	7.8	0.0861551	12	1.74752	23	17.9	4.698	62.4	12.4	0.0793364
4	5.2	129	16.5	0.0025948	1	0	0	0	5.2	123	1	0
5	5.312	145	18.5	0.0854431	8	1.24635	16.4	12.7	5.312	141.1	8	0.0879191
6	5.438	163	20.8	0.017561	2	0.0413341	0.5	0.4	5.438	162.8	1.5	0.0150693
7	5.528	176	22.5	0.0183585	7	0.225055	3	2.3	5.528	178	6.6	0.0192034
8	5.689	199	25.4	0.020966	5	0.147576	1.9	1.5	5.689	199.9	5	0.0165841
9	5.85	222	28.4	0.0456749	6	0.46684	6.1	4.8	5.85	219.7	5.6	0.0467896
10	5.934	238	30.4	0.0104252	10	0.226741	3	2.3	5.934	242.6	10.3	0.0124697
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.043	259	33.1	0.0325069	2	0.080603	1.1	0.8	6.043	259	1.6	0.0282819
12	6.096	269	34.4	0.006241	9	0.167244	2.2	1.7	6.096	279.9	8.8	0.0106775
13	6.205	290	37.1	0.0269296	7	0.204752	2.7	2.1	6.205	293	6.6	0.017414
14	6.346	317	40.5	0.0398134	15	0.551236	7.2	5.6	6.346	316.4	14.9	0.0209098
15	6.466	340	43.5	0.0485476	4	0.253581	3.3	2.6	6.466	340.2	3.6	0.0398737
16	6.55	356	45.5	0.0248983	4	0.136922	1.8	1.4	6.55	357.1	4.1	0.0186996
17	7.35	456	58.3	0.0332478	5	0.308548	4.1	3.2	7.35	456.7	5.5	0.0317536
18	8.15	580	74.2	0.0130624	24	0.627369	8.2	6.4	8.15	572.1	24	0.0147484
19	8.45	647	82.7	0.0318407	6	0.32186	4.2	3.3	8.45	646.8	6.2	0.0292292
20	8.65	697	89.1	0.029887	4	0.220952	2.9	2.3	8.65	697.6	4.4	0.0282787
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	9.3	759	97.1	0.0284927	5	0.264013	3.5	2.7	9.3	758	5.2	0.0285273

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.493	30	3.8	0.0237263	5	0.215228	2.4	1.7	4.493	28	5.3	0.0228436
2	4.649	52	6.7	0.0838907	11	1.57362	17.4	12.2	4.649	52.1	11.4	0.0777657
3	5.211	131	16.8	0.0368042	3	0.184185	2	1.4	5.211	130.9	3	0.035141
4	5.319	146	18.7	0.237279	5	1.84149	20.3	14.2	5.319	145.9	4.7	0.219283
5	5.448	164	21	0.0893526	8	1.24254	13.7	9.6	5.448	162.2	8.4	0.0834779
6	5.577	182	23.3	0.0767369	4	0.506477	5.6	3.9	5.577	181.4	4	0.0705628
7	5.735	204	26.2	0.0345433	3	0.184893	2	1.4	5.735	203.8	3.1	0.0338956
8	5.865	223	28.6	0.142285	4	0.944055	10.4	7.3	5.865	223	4	0.133205
9	5.957	241	30.9	0.03963	8	0.550427	6.1	4.3	5.957	238.2	8.2	0.0379479
10	6.022	254	32.6	0.0064704	5	0.0311315	0.3	0.2	6.022	257.9	4.8	0.0036865
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.078	265	34	0.0287188	4	0.180276	2	1.4	6.078	263.6	3.9	0.0263503
12	6.129	275	35.3	0.0172192	4	0.0986862	1.1	0.8	6.129	274.9	3.6	0.0155299
13	6.241	297	38.1	0.050084	5	0.391678	4.3	3	6.241	295.9	4.9	0.0455322
14	6.317	312	40	0.044707	5	0.332837	3.7	2.6	6.317	310.5	5.3	0.0354788
15	6.449	338	43.3	0.0613392	8	0.777994	8.6	6	6.449	338.1	8	0.0545826

PeakCounter: 15

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	37	4.7	0.0404194	5	0.328819	9	4.7	4.55	37.7	5.1	0.0363747
2	5.2	132	16.9	0.0196461	12	0.332353	9.1	4.8	5.2	132.3	12	0.0156242
3	5.85	220	28.1	0.0220044	7	0.233596	6.4	3.3	5.85	221.3	6.6	0.0198625
4	6.55	362	46.3	0.0170828	8	0.208449	5.7	3	6.55	359.1	7.9	0.0148033
5	7.35	459	58.7	0.0651236	4	0.452925	12.3	6.5	7.35	458.1	4.2	0.0603086
6	8.15	583	74.6	0.0476131	8	0.609803	16.6	8.7	8.15	582.4	7.7	0.0447652
7	8.45	648	82.9	0.0671564	5	0.657638	17.9	9.4	8.45	649.4	5.5	0.0678578
8	8.65	702	89.8	0.0680684	4	0.480558	13.1	6.9	8.65	701.6	4.4	0.0610606
9	9.3	767	98.1	0.0486181	4	0.367821	10	5.3	9.3	766.7	4.5	0.0465138

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 79

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 13.400 5.200 7.200 5.850 6.000 6.550 9.900 7.350 7.900 8.150 11.000 8.450 21.100 8.650 10.800 9.300 12.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.352 1.300 4.538 19.300 5.168 1.600 5.276 18.600 5.379 11.800 5.510 2.700 5.668 3.000 5.819 10.500 5.931 9.000 5.998 0.900 6.066 0.300 6.097 0.600 6.207 4.900 6.285 1.900 6.337 2.400 6.409 11.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 16 Return to Lane Navigator
4.550 12.000 5.200 6.700 5.850 3.100 6.550 19.700 7.350 8.200 8.150 13.200 8.450 13.400 8.650 9.100 9.300 14.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.476 4.100 4.550 0.800 4.698 23.000 5.200 0 5.312 16.400 5.438 0.500 5.528 3.000 5.689 1.900 5.850 6.100 5.934 3.000 6.043 1.100 6.096 2.200 6.205 2.700 6.346 7.200 6.466 3.300 6.550 1.800 7.350 4.100 8.150 8.200 8.450 4.200 8.650 2.900 9.300 3.500 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 21 Return to Lane Navigator
4.493 2.400 4.649 17.400 5.211 2.000 5.319 20.300 5.448 13.700 5.577 5.600 5.735 2.000 5.865 10.400 5.957 6.100 6.022 0.300 6.078 2.000 6.129 1.100 6.241 4.300 6.317 3.700 6.449 8.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 15 Return to Lane Navigator
4.550 9.000 5.200 9.100 5.850 6.400 6.550 5.700 7.350 12.300 8.150 16.600 8.450 17.900 8.650 13.100 9.300 10.000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Atlantic Cod ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Cod ---- Go to Display Gel Lane Navigator.