RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Cod ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Gadus morhua
FDA Seafood List Common Name: Atlantic Cod
FDA Seafood List Market Name: Cod
Unique IEF Gel Code: 19940912gel04
Unique Web Code: acg01Srr
Tissue Aliquot Code: gadumorh H94-002 #04a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel	Comparison Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Co-Migrated	Lane c Specimen	Lane d pI Markers	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	29	2.5	0.159758	4	1.26228	5.5	4.4	3.5	30.4	4.4	0.161296
2	4.55	181	15.8	0.220039	5	1.92125	8.4	6.7	4.55	181	5.2	0.207548
3	5.2	261	22.9	0.289556	5	2.55349	11.1	9	5.2	260.3	5	0.28905
4	5.85	405	35.5	0.374677	4	2.6105	11.4	9.2	5.85	404.8	3.9	0.376341
5	6.55	594	52	0.285326	5	2.74208	12	9.6	6.55	593	5.5	0.28225
6	6.85	631	55.3	0.104608	8	1.29741	5.7	4.5	6.85	629.8	7.8	0.0939678
7	7.35	726	63.6	0.272925	6	2.80909	12.3	9.8	7.35	724.5	5.8	0.271499
8	8.15	885	77.5	0.0695328	13	1.54004	6.7	5.4	8.15	883.1	12.6	0.0689668
9	8.45	956	83.7	0.0970147	8	1.35978	5.9	4.8	8.45	955.7	8.3	0.0925929
10	8.65	996	87.2	0.0938559	6	0.888857	3.9	3.1	8.65	995.6	5.7	0.0875636
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1125	98.5	0.368517	6	3.93889	17.2	13.8	9.3	1124.3	6.2	0.359687

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	37	3.2	0.188727	8	2.07854	4.3	3.7	3.5	36.6	7.9	0.148943
2	3.921	94	8.2	0.0484676	6	0.515305	1.1	0.9	3.921	94.8	6.4	0.0457376
3	4.114	120	10.5	0.581696	4	4.27265	8.8	7.6	4.114	120.7	4	0.603017
4	4.306	146	12.8	0.387796	3	2.40337	5	4.3	4.306	145.7	3.5	0.391686
5	4.483	170	14.9	0.0483882	5	0.386179	0.8	0.7	4.483	168.2	4.8	0.0456773
6	4.55	179	15.7	0.217035	4	1.46605	3	2.6	4.55	179	4.3	0.191611
7	4.837	213	18.7	0.0559826	3	0.303845	0.6	0.5	4.837	211.8	2.7	0.0643621
8	5.006	233	20.4	0.0524349	8	0.586219	1.2	1	5.006	231.7	7.8	0.0424088
9	5.09	243	21.3	0.0504597	4	0.290729	0.6	0.5	5.09	243.6	4	0.040807
10	5.2	256	22.5	0.186413	3	1.08663	2.2	1.9	5.2	255.4	3.5	0.17558
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.255	268	23.5	0.443429	4	3.13715	6.5	5.6	5.255	268.4	3.7	0.475342
12	5.319	282	24.7	0.365309	2	1.46358	3	2.6	5.319	282.2	2.4	0.347883
13	5.397	299	26.2	0.141799	4	0.921274	1.9	1.6	5.397	298.7	3.8	0.13594
14	5.452	311	27.3	0.0214232	5	0.218912	0.5	0.4	5.452	308.2	4.8	0.0259652
15	5.507	323	28.3	0.0108663	6	0.0985411	0.2	0.2	5.507	324.6	5.7	0.00974211
16	5.58	339	29.7	0.0186205	3	0.0958741	0.2	0.2	5.58	338.8	3	0.0181527
17	5.69	363	31.8	0.0204721	4	0.151411	0.3	0.3	5.69	363.7	4.3	0.0199073
18	5.786	384	33.7	0.386088	4	2.79128	5.8	5	5.786	384.9	4.2	0.378962
19	5.85	398	34.9	0.212803	4	1.54939	3.2	2.7	5.85	397.8	4.3	0.204711
20	5.894	409	35.9	0.284892	5	2.43069	5	4.3	5.894	409.8	5	0.276391
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	5.985	432	37.9	0.313259	3	1.65753	3.4	2.9	5.985	432.5	3	0.314145
22	6.017	440	38.6	0.162781	3	0.647727	1.3	1.1	6.017	439.7	2.8	0.130827
23	6.053	449	39.4	0.0401022	7	0.654393	1.4	1.2	6.053	444.6	6.7	0.0550325
24	6.124	467	41	0.0803976	2	0.322028	0.7	0.6	6.124	466.8	2.2	0.0816795
25	6.172	479	42	0.285103	2	1.18829	2.5	2.1	6.172	479.4	2.3	0.297867
26	6.303	512	44.9	0.0591609	9	0.7631	1.6	1.4	6.303	514.5	8.6	0.0500708
27	6.411	539	47.3	0.0647776	13	1.3934	2.9	2.5	6.411	540.2	12.8	0.0615639
28	6.451	549	48.2	0.0921098	4	0.338093	0.7	0.6	6.451	549.1	3.6	0.0533167
29	6.55	574	50.4	0.122323	6	1.31793	2.7	2.3	6.55	573.7	6.1	0.12121
30	6.85	615	53.9	0.101408	5	0.768748	1.6	1.4	6.85	614.8	4.7	0.0924792
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.167	674	59.1	0.024744	4	0.166856	0.3	0.3	7.167	673.5	3.9	0.0242662
32	7.35	708	62.1	0.291226	6	2.7559	5.7	4.9	7.35	708.2	5.6	0.277134
33	7.524	743	65.2	0.115517	9	1.69404	3.5	3	7.524	741.3	9	0.105737
34	8.15	869	76.2	0.0368219	18	1.61293	3.3	2.9	8.15	879.7	18	0.0505563
35	8.45	937	82.2	0.0429423	9	0.666731	1.4	1.2	8.45	939	8.7	0.0429914
36	8.65	984	86.3	0.0823761	5	0.735125	1.5	1.3	8.65	983.7	5.1	0.0811352
37	9.3	1124	98.6	0.478069	7	5.37343	11.1	9.5	9.3	1122.1	6.9	0.438585

PeakCounter: 37

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.924	93	8.2	0.0528162	7	0.632612	2.1	1.8	3.924	94	6.9	0.0513766
2	4.141	123	10.8	0.574724	4	4.45967	15	12.4	4.141	123.3	4.2	0.597791
3	4.336	150	13.2	0.415415	4	2.67227	9	7.4	4.336	149.6	3.6	0.42254
4	4.51	174	15.3	0.0241037	5	0.179352	0.6	0.5	4.51	173.7	5	0.0204238
5	4.629	189	16.6	0.0356558	9	0.544235	1.8	1.5	4.629	191.7	8.6	0.0356168
6	4.887	220	19.3	0.0573502	3	0.382868	1.3	1.1	4.887	218.4	3	0.0730661
7	5.054	240	21.1	0.0290359	3	0.184842	0.6	0.5	5.054	240.3	3.4	0.030784
8	5.137	250	22	0.0342983	4	0.240159	0.8	0.7	5.137	250.7	3.8	0.0357322
9	5.224	263	23.1	0.0379841	4	0.244534	0.8	0.7	5.224	260.9	3.8	0.0367044
10	5.277	275	24.2	0.480177	4	3.20193	10.8	8.9	5.277	275.6	3.6	0.506709
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.344	290	25.5	0.405759	2	1.56052	5.2	4.3	5.344	289.9	2.2	0.394499
12	5.419	307	27	0.170344	3	0.863556	2.9	2.4	5.419	306.7	2.9	0.167759
13	5.463	317	27.9	0.0288532	4	0.219769	0.7	0.6	5.463	316.3	4.4	0.0280661
14	5.521	330	29	0.0145045	2	0.0572029	0.2	0.2	5.521	329.9	2.5	0.012992
15	5.596	347	30.5	0.0379971	3	0.171863	0.6	0.5	5.596	347.4	2.8	0.0348939
16	5.644	358	31.5	0.0222589	4	0.14495	0.5	0.4	5.644	357.4	3.8	0.021253
17	5.693	369	32.4	0.0154598	5	0.13808	0.5	0.4	5.693	367.5	5	0.0154446
18	5.773	387	34	0.61539	3	2.9555	9.9	8.2	5.773	387.6	2.9	0.575341
19	5.884	413	36.3	0.42375	4	2.46838	8.3	6.9	5.884	412.8	3.9	0.360008
20	5.94	427	37.5	0.0476421	18	1.76581	5.9	4.9	5.94	437.1	18	0.0553483
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	5.984	438	38.5	0.437611	2	1.0596	3.6	2.9	5.984	437.8	1.7	0.361531
22	6.016	446	39.2	0.107579	3	0.306124	1	0.9	6.016	444.4	2.8	0.062509
23	6.057	456	40.1	0.00387756	1	0	0	0	6.057	452	1	0
24	6.077	461	40.5	0.00851534	1	0	0	0	6.077	458	1	0
25	6.121	472	41.5	0.121974	3	0.510383	1.7	1.4	6.121	472.5	2.6	0.110666
26	6.161	482	42.4	0.364349	2	1.17221	3.9	3.3	6.161	482.1	1.8	0.362483
27	6.289	514	45.2	0.0601792	2	0.219768	0.7	0.6	6.289	513.7	2.1	0.0589038
28	6.382	537	47.2	0.0448422	7	0.539559	1.8	1.5	6.382	538.1	7	0.0432301
29	6.43	549	48.2	0.060674	8	0.717386	2.4	2	6.43	549.9	7.5	0.0536395
30	7.243	693	60.9	0.0493157	13	1.10876	3.7	3.1	7.243	692.3	13.2	0.0472362
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	7.419	727	63.9	0.0859337	7	1.06032	3.6	3	7.419	726.8	6.9	0.0868861

PeakCounter: 31

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 8
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	35	3.1	0.221311	12	4.2595	17.5	13.8	3.5	35.3	12.5	0.192772
2	4.55	183	16.1	0.296683	4	1.99261	8.2	6.5	4.55	183	4.2	0.268344
3	5.2	262	23	0.36643	4	2.62116	10.7	8.5	5.2	261.4	4.1	0.3566
4	5.85	414	36.3	0.502853	3	2.44855	10	7.9	5.85	413.6	2.7	0.517762
5	6.55	587	51.5	0.300909	5	2.34778	9.6	7.6	6.55	585.6	4.7	0.281299
6	6.85	627	55	0.109984	6	1.11447	4.6	3.6	6.85	627.1	6.4	0.0979372
7	7.35	721	63.3	0.270182	5	2.29391	9.4	7.4	7.35	720.3	4.9	0.264503
8	8.15	884	77.6	0.0679446	11	1.29706	5.3	4.2	8.15	883	11	0.0662551
9	8.45	949	83.3	0.114657	8	1.54027	6.3	5	8.45	949.3	7.8	0.111267
10	8.65	990	86.9	0.0981689	5	0.920299	3.8	3	8.65	989.9	5.5	0.0944124
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1124	98.7	0.50919	4	3.56333	14.6	11.5	9.3	1123.1	4.1	0.492893

PeakCounter: 11

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 9
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.944	102	9	0.0571614	6	0.567073	1.7	1.5	3.944	102.6	6.2	0.0516709
2	4.131	129	11.3	0.578831	4	4.47139	13.6	11.5	4.131	130	4.2	0.605449
3	4.318	156	13.7	0.421233	4	2.75418	8.4	7.1	4.318	155.9	3.6	0.426972
4	4.477	179	15.7	0.0285903	5	0.219568	0.7	0.6	4.477	179.1	4.8	0.0259781
5	4.618	198	17.4	0.0382815	9	0.629214	1.9	1.6	4.618	197.9	9.1	0.0391987
6	4.825	224	19.7	0.082903	3	0.42289	1.3	1.1	4.825	223.8	3.1	0.0771754
7	4.985	244	21.4	0.0434176	5	0.298605	0.9	0.8	4.985	243.1	4.5	0.0372386
8	5.064	254	22.3	0.0325161	2	0.128841	0.4	0.3	5.064	254.2	2.4	0.0309108
9	5.152	265	23.3	0.0392965	5	0.32299	1	0.8	5.152	264.6	5.2	0.034865
10	5.2	271	23.8	0.0275377	2	0.0563886	0.2	0.1	5.2	269.5	2.4	0.0130563
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.239	280	24.6	0.527021	3	2.96481	9	7.6	5.239	280.7	3.2	0.525712
12	5.316	298	26.2	0.427247	3	1.90203	5.8	4.9	5.316	297.8	2.7	0.400913
13	5.415	321	28.2	0.153327	3	0.936929	2.8	2.4	5.415	320.4	3.5	0.152649
14	5.457	331	29.1	0.00919127	6	0.104278	0.3	0.3	5.457	328.5	5.9	0.00998882
15	5.5	341	29.9	0.00988997	2	0.0268078	0.1	0.1	5.5	341.5	1.7	0.00903124
16	5.552	353	31	0.0156396	2	0.0526225	0.2	0.1	5.552	353	1.9	0.0153584
17	5.586	361	31.7	0.0375584	2	0.0732419	0.2	0.2	5.586	361	1.6	0.0258274
18	5.62	369	32.4	0.0373667	11	0.538316	1.6	1.4	5.62	371	11.4	0.0266492
19	5.689	385	33.8	0.0134734	1	0	0	0	5.689	356	1	0
20	5.741	397	34.9	0.538972	4	3.21725	9.8	8.3	5.741	397.8	3.6	0.511075
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	5.856	424	37.2	0.333407	4	2.32271	7.1	6	5.856	423.8	4.1	0.321946
22	5.913	438	38.5	0.0403019	4	0.250119	0.8	0.6	5.913	438.8	3.7	0.0386301
23	5.967	451	39.6	0.401736	3	1.81014	5.5	4.7	5.967	450.7	2.6	0.38675
24	6.004	460	40.4	0.0910531	3	0.385466	1.2	1	6.004	460.1	2.5	0.0865016
25	6.028	466	40.9	0.0170846	1	0.0311296	0.1	0.1	6.028	466.7	1.2	0.0141644
26	6.065	475	41.7	0.0277175	3	0.12386	0.4	0.3	6.065	474.7	2.6	0.0268217
27	6.122	489	42.9	0.147015	2	0.626446	1.9	1.6	6.122	489.3	2.4	0.147569
28	6.155	497	43.6	0.362807	2	1.37162	4.2	3.5	6.155	497.3	2.1	0.364443
29	6.286	529	46.4	0.0639981	6	0.600428	1.8	1.5	6.286	530.1	6.3	0.0539941
30	6.429	564	49.5	0.0756481	16	2.02992	6.2	5.2	6.429	559.7	16.1	0.0711369
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
31	6.951	652	57.2	0.0061891	5	0.0386387	0.1	0.1	6.951	652.7	4.6	0.00478821
32	7.041	669	58.7	0.0167227	4	0.100345	0.3	0.3	7.041	670.2	4.2	0.013536
33	7.233	705	61.9	0.0682616	16	1.86507	5.7	4.8	7.233	704	16.3	0.0644397
34	7.394	736	64.6	0.106414	9	1.70008	5.2	4.4	7.394	735.6	9.4	0.102319

PeakCounter: 34

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 8
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 10
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	3.5	38	3.3	0.216826	14	4.66329	17.8	14.3	3.5	38.6	13.9	0.189827
2	4.55	193	17	0.297419	5	2.31697	8.9	7.1	4.55	193.3	4.8	0.273021
3	5.2	277	24.4	0.393918	4	2.76337	10.6	8.5	5.2	276.9	4.1	0.381318
4	5.85	428	37.6	0.522209	3	2.66356	10.2	8.2	5.85	428.3	2.9	0.527244
5	6.55	597	52.5	0.323864	4	2.36941	9.1	7.3	6.55	596	4.4	0.303232
6	6.85	636	55.9	0.113838	6	1.12014	4.3	3.4	6.85	636.9	6.1	0.103419
7	7.35	730	64.2	0.282291	5	2.2887	8.8	7	7.35	729.4	4.7	0.275373
8	8.15	892	78.5	0.0870386	10	1.55645	6	4.8	8.15	891.7	10.3	0.085181
9	8.45	956	84.1	0.148354	7	1.83609	7	5.6	8.45	956.2	7.2	0.143704
10	8.65	996	87.6	0.113681	6	1.0806	4.1	3.3	8.65	996	5.6	0.109433
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	9.3	1120	98.5	0.480694	4	3.49735	13.4	10.7	9.3	1119.5	4.2	0.474283

PeakCounter: 11

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK CO SP MK SP MK

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 3.50 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 135

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
3.500 5.500 4.550 8.400 5.200 11.100 5.850 11.400 6.550 12.000 6.850 5.700 7.350 12.300 8.150 6.700 8.450 5.900 8.650 3.900 9.300 17.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 3 LANE TYPE: pI Markers ACTUAL GEL LANE: 5 PeakCount: 11 Return to Lane Navigator
3.500 4.300 3.921 1.100 4.114 8.800 4.306 5.000 4.483 0.800 4.550 3.000 4.837 0.600 5.006 1.200 5.090 0.600 5.200 2.200 5.255 6.500 5.319 3.000 5.397 1.900 5.452 0.500 5.507 0.200 5.580 0.200 5.690 0.300 5.786 5.800 5.850 3.200 5.894 5.000 5.985 3.400 6.017 1.300 6.053 1.400 6.124 0.700 6.172 2.500 6.303 1.600 6.411 2.900 6.451 0.700 6.550 2.700 6.850 1.600 7.167 0.300 7.350 5.700 7.524 3.500 8.150 3.300 8.450 1.400 8.650 1.500 9.300 11.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 4 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 6 PeakCount: 37 Return to Lane Navigator
3.924 2.100 4.141 15.000 4.336 9.000 4.510 0.600 4.629 1.800 4.887 1.300 5.054 0.600 5.137 0.800 5.224 0.800 5.277 10.800 5.344 5.200 5.419 2.900 5.463 0.700 5.521 0.200 5.596 0.600 5.644 0.500 5.693 0.500 5.773 9.900 5.884 8.300 5.940 5.900 5.984 3.600 6.016 1.000 6.057 0 6.077 0 6.121 1.700 6.161 3.900 6.289 0.700 6.382 1.800 6.430 2.400 7.243 3.700 7.419 3.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 5 LANE TYPE: Specimen ACTUAL GEL LANE: 7 PeakCount: 31 Return to Lane Navigator
3.500 17.500 4.550 8.200 5.200 10.700 5.850 10.000 6.550 9.600 6.850 4.600 7.350 9.400 8.150 5.300 8.450 6.300 8.650 3.800 9.300 14.600 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 6 LANE TYPE: pI Markers ACTUAL GEL LANE: 8 PeakCount: 11 Return to Lane Navigator
3.944 1.700 4.131 13.600 4.318 8.400 4.477 0.700 4.618 1.900 4.825 1.300 4.985 0.900 5.064 0.400 5.152 1.000 5.200 0.200 5.239 9.000 5.316 5.800 5.415 2.800 5.457 0.300 5.500 0.100 5.552 0.200 5.586 0.200 5.620 1.600 5.689 0 5.741 9.800 5.856 7.100 5.913 0.800 5.967 5.500 6.004 1.200 6.028 0.100 6.065 0.400 6.122 1.900 6.155 4.200 6.286 1.800 6.429 6.200 6.951 0.100 7.041 0.300 7.233 5.700 7.394 5.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 7 LANE TYPE: Specimen ACTUAL GEL LANE: 9 PeakCount: 34 Return to Lane Navigator
3.500 17.800 4.550 8.900 5.200 10.600 5.850 10.200 6.550 9.100 6.850 4.300 7.350 8.800 8.150 6.000 8.450 7.000 8.650 4.100 9.300 13.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 8 LANE TYPE: pI Markers ACTUAL GEL LANE: 10 PeakCount: 11 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Atlantic Cod ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Atlantic Cod ---- Go to Display Gel Lane Navigator.