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Germination Test Methods |
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Ornamentals: M. Widrlechner, Curator
Click here for information on the seed testing papers we use. Contact Curator Mark Widrlechner.
ENO: 493243 Actaea |
Duration |
Temperature |
Fluorescent Lights |
12 hours |
20 ºC |
ON |
12 hours |
8 ºC |
OFF |
Seeds are soaked in 1 mM GA3 overnight. Rinse seeds with tap water. Seeds are placed on top of 3 blotter papers (1 blue, 2 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator with temperatures alternating between 20 ºC with light for 12 hours and 8 ºC in darkness for 12 hours per 24 hour cycle. Leave at this regimen for many days. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts as needed. |
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ENO: 493218 Agastache
|
Duration |
Temperature |
Fluorescent Lights |
12 hours |
25 ºC |
ON |
12 hours |
15 ºC |
OFF |
Seeds are placed on 3 blotter papers (2 blue, 1 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 8 and 16 days after start of test. |
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ENO: 493226 Alcea, Althea |
Duration |
Temperature |
Fluorescent Lights |
12 hours |
20 ºC |
ON |
12 hours |
20 ºC |
OFF |
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper seeds 1 white paper (bottom) 1 blue paper
Let sit overnight at room temperature. Transfer to germinator set at constant 20 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts on 5 and 18 days after start of test. Chip remaining seed. Record final count after 5 more days. |
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ENO: 492151 Calendula |
Duration |
Temperature |
Fluorescent Lights |
12 hours |
25 ºC |
ON |
12 hours |
15 ºC |
OFF |
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"); substrate should be on drier side. Let sit overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test. |
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ENO: 493795 Centranthus |
Duration |
Temperature |
Fluorescent Lights |
12 hours |
25 ºC |
ON |
12 hours |
15 ºC |
OFF |
Seeds are placed on 3 blotter papers (1 blue, 2 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). Let seeds imbibe overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur on 7, 14, and 21 days after start of test. |
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ENO: 493796 Comarum
|
Duration |
Temperature |
Fluorescent Lights |
21 days |
4 ºC |
--- |
12 hours |
25 ºC |
ON |
12 hours |
15 ºC |
OFF |
Seeds are placed on top of 3 blue blotter (1 blue, 2 white – in contact with seeds) papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 21 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur on 7, 14, and 21 days after start of test. |
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ENO: 493228 Dasiphora, Glebionis, Hyssopus
|
Duration |
Temperature |
Fluorescent Lights |
7 days |
4 ºC |
--- |
12 hours |
25 ºC |
ON |
12 hours |
15 ºC |
OFF |
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 7 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur on 7, 14, and 21 days after start of germination test. |
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ENO: 490482 Dracocephalum, Matricaria |
Duration |
Temperature |
Fluorescent Lights |
12 hours |
20 ºC |
ON |
12 hours |
20 ºC |
OFF |
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator set at constant 20 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test. |
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ENO: 493794 Anthemis, Duchesnea, Lythrum, Rumex, Sphaeralcea |
Duration |
Temperature |
Fluorescent Lights |
12 hours |
30 ºC |
ON |
12 hours |
20 ºC |
OFF |
Seeds are placed on top of three blotter papers (1 blue, 2 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). Let seeds imbibe overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test. |
TOP
ENO: 491056 Echinacea |
Duration |
Temperature |
Fluorescent Lights |
28 days |
4 ºC |
--- |
12 hours |
25 ºC |
ON |
12 hours |
25 ºC |
OFF |
Soak seeds ovenight in 1 mM Ethephon. Transfer to germination boxes: seeds are placed on top of 2 blue blotter papers moistened with tap water. Prechill at 4 ºC for 28 days. Transfer to germinator with temperature set to a constant 25 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
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ENO: 493957 Heteranthemis |
Duration |
Temperature |
Fluorescent Lights |
12 hours |
15 ºC |
ON |
12 hours |
15 ºC |
OFF |
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator set at constant 20 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test. |
TOP
ENO: 493793 Heteropappus
|
Duration |
Temperature |
Fluorescent Lights |
28 days |
4 ºC |
--- |
12 hours |
25 ºC |
ON |
12 hours |
15 ºC |
OFF |
Seeds are placed on top of 3 blotter papers (1 blue, 2 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 28 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
ENO: 491385 Hyoscymous, Physocarpus |
Duration |
Temperature |
Fluorescent Lights |
21 days |
4 ºC |
--- |
12 hours |
25 ºC |
ON |
12 hours |
15 ºC |
OFF |
Seeds are placed between blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper seeds 1 white paper (bottom) 1 blue paper
Let sit overnight at room temperature. Prechill for 21 days. Remove top blotter paper and transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
ENO: 493229 Hypericum, Monarda |
Duration |
Temperature |
Fluorescent Lights |
12 hours |
30 ºC |
ON |
12 hours |
20 ºC |
OFF |
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test. |
TOP
ENO: 493958 Lappula |
Duration |
Temperature |
Fluorescent Lights |
14 days |
4 ºC |
--- |
12 hours |
15 ºC |
ON |
12 hours |
15 ºC |
OFF |
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 14 days. Transfer to germinator with temperature set to a constant 15 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
ENO: 490498 Lavatera, Malva |
Duration |
Temperature |
Fluorescent Lights |
Constant |
Room Temp |
DARK |
Seeds are placed between 2 moist blotter papers and allowed to imbibe water overnight. Seeds are then chipped and placed between blotter papers moistened with tap water in the following arrangement:
(top) 1 blue paper seeds 1 white paper (bottom) 1 blue paper
These are kept at room temperature in the dark. Replication and sample size: 4 reps of 50 sd for total of 200 seeds. First count occurs in 4 to 8 days after chipping seed. Germination counts occur weekly after that. |
TOP
ENO: 493959 Malope |
Duration |
Temperature |
Fluorescent Lights |
12 hours |
15 ºC |
ON |
12 hours |
15 ºC |
OFF |
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper seeds 1 white paper (bottom) 1 blue paper
Let sit overnight at room temperature. Transfer to germinator set at constant 15 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
ENO: 490496 Origanum |
Duration |
Temperature |
Fluorescent Lights |
Constant |
15 ºC |
--- |
Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:
(top) 1 blue paper seeds 1 white paper (bottom) 1 blue paper
Let sit overnight at room temperature. Transfer to germinator with temperature set to a constant 15 ºC. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
ENO: 493227 Potentilla |
Duration |
Temperature |
Fluorescent Lights |
21 days |
4 ºC |
--- |
7 days |
10 ºC |
OFF |
12 hours |
20 ºC |
ON |
12 hours |
20 ºC |
OFF |
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue – in contact with seeds) moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 21 days. Transfer to germinator with temperature set to a constant 10 ºC and darkness for 7 days. Then transfer to germinator with temperature set for 20 C with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after transfer to 20 ºC germinator. |
TOP
ENO: 493225 Prunella |
Duration |
Temperature |
Fluorescent Lights |
12 hours |
30 ºC |
OFF |
12 hours |
20 ºC |
OFF |
Seeds are placed between blotter papers moistened with tap water in the following arrangement:
(top) 1 blue paper seeds 1 white paper (bottom) 1 blue paper
Let sit overnight. Transfer to germinator with temperatures alternating between 30 ºC for 12 hours and 20 ºC for 12 hours per 24 hour cycle, no lights. Replication and sample size: 4 reps of 50 sd for total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
ENO: 490484 Sanvitalia
|
Duration |
Temperature |
Fluorescent Lights |
7 days |
4 ºC |
--- |
12 hours |
25 ºC |
ON |
12 hours |
15 ºC |
OFF |
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 7 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur on 7, 14, and 21 days after start of germination test. |
TOP
ENO: 493797 Scrophularia
|
Duration |
Temperature |
Fluorescent Lights |
21 days |
4 ºC |
--- |
12 hours |
30 ºC |
ON |
12 hours |
20 ºC |
OFF |
Seeds are placed on top of 3 blotter papers (1 blue, 2 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 21 days. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
ENO: 490493 Sibiraea, Sorbaria, Spiraea
|
Duration |
Temperature |
Fluorescent Lights |
28 days |
4 ºC |
--- |
12 hours |
30 ºC |
ON |
12 hours |
20 ºC |
OFF |
Seeds are placed on top of 3 blotter papers (1 blue, 1 white, 1 blue – in contact with seeds) moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 28 days. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
ENO: 493960 Simsia |
Duration |
Temperature |
Fluorescent Lights |
7 days |
4 ºC |
--- |
12 hours |
30 ºC |
ON |
12 hours |
20 ºC |
OFF |
Seeds are placed in folded paper towels1 moistened with tap water and placed in plastic tubs. Let sit overnight at room temperature. Prechill at 4 ºC for 7 days. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test.
1Seeds are wrapped in 2 sheets of germination toweling and replicated four times. The four replications are rubber banded together and eight accessions are placed in plastic tubs. Towels are kept moist with tap water during the germination period. |
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ENO: 490499 Spergula |
Duration |
Temperature |
Fluorescent Lights |
7 days |
4 ºC |
--- |
12 hours |
15 ºC |
ON |
12 hours |
15 ºC |
OFF |
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 7 days. Transfer to germinator with temperature set to a constant 15 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
TOP
ENO: 492545 Tanacetum
|
Duration |
Temperature |
Fluorescent Lights |
12 hours |
25 ºC |
ON |
12 hours |
15 ºC |
OFF |
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur on 7, 14, and 21 days after start of germination test. |
TOP
ENO: 490497 Vaccaria |
Duration |
Temperature |
Fluorescent Lights |
7 days |
4 ºC |
--- |
12 hours |
15 ºC |
ON |
12 hours |
15 ºC |
OFF |
Seeds are placed on top of 2 blue blotter papers moistened with with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Prechill at 4 ºC for 7 days. Transfer to germinator with temperature set to a constant 15 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. |
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