Project Number: 1275-21220-221-19
Project Type: Specific Cooperative Agreement

Start Date: Jun 01, 2007
End Date: Sep 30, 2008

Objective:
To identify and characterize proteins, gene transcripts and corresponding genes associated with soybean root infection by soybean cyst nematode (SCN). Molecular mechanisms that regulate pathogen-induced gene expression in the host plant will be identified. The goal is to reduce pathogen infectivity and increase productivity of soybean through genetic engineering of processes that will increase resistance to SCN infection.

Approach:
Several experimental approaches, including subtraction libraries, micro arrays, proteomics and screening for cell wall hydrolase have been used to identify SCN-induced gene expression. Cell-specific expression will be determined by in situ hybridization and hairy root transformation with promoter::reporter gene (e.g., GUS, GFP, etc) constructs. The promoters for selected genes will be analyzed to identify cis-acting elements functional in SCN-specific expression. In addition, the role of the plant hormone ethylene in the development of nematode feeding structure will be further elucidated.