Project Number: 1275-21000-263-20
Project Type: Reimbursable

Start Date: Apr 01, 2005
End Date: Mar 30, 2008

Objective:
The first objective is to develop at least 500 new SNP DNA markers and to map these markers in the existing Univ. of Utah Mapping Population so as to substantially increase in the density of DNA markers on the soybean genome map. The second objective is to target the development of new SNP DNA markers to regions of the genome associated with seed protein concentration.

Approach:
The development of SNP DNA markers will focus on the discovery of markers from bacterial artificial chromosome (BAC)-end DNA sequence data which is available in GenBank. Polymerase chain reaction primers will be developed using the GenBank DNA sequence data. The SNP markers will be developed via the sequence analysis of a set of six previously identified soybean genotypes which are particularly useful for the discovery of SNPs. Genetic mapping in the two of the University of Utah recombinant inbred line mapping populations will be used to position the markers on the soybean genome map along with more than 1000 SSR markers that have been previously mapped. Using this approach as well as targeted marker development we will identify additional SNP markers in close proximity to genes that provide resistance to elevated seed protein in order to select new varieties with improved resistance and seed quality characteristics.